Status Persentase Tutupan Karang Scleractinia Di Pulau Bunaken (Taman Nasional Bunaken) Dan Di Pantai Malalayang, Pesisir Kota Manado

Karang scleractinia atau karang batu merupakan karang pembentuk terumbu. Sehingga penentuan status kondisi karang batu menjadi bagian penting dan sangat menentukan peran ekologis dan ekonomis ekosistim terumbu karang. Salah satu parameter untuk mengetahui kondisi karang batu yaitu persentase tutupan di alam. Penelitian dilakukan menggunakan metode deskriptif kuantitatif dengan tehnik pengambilan data dengan menggunakan LIT (Line InterceptTransect) yang selanjutnya dianalisis dengan Persentase Tutupan Karang setelah data diolah dengan ‘benthic life form\u27. Parameter lingkungan berupa salinitas dan suhu air serta kecerahan juga diukur.Hasil penelitian menunjukkan bahwa persentase tutupan di perairan pantai Malalayang Kota Manado dalam status kategori ‘poor\u27 (Buruk) khususnya di kedalaman 3 dan 5 meter yaitu pada kisaran antara 0-24,9 %, sekalipun pada kedalaman 10 meter hanya kategori ‘good\u27 (Baik), pada kisaran antara 50 – 69,9 %.. Sedangkan di stasiun penelitian Pulau Bunaken baik di bagian selatan yaitu Stasiun Fukui dan bagian utara pulau yaitu Stasiun Pangalisang hasilnya sebagian besar ‘excellent\u27 (sangat baik) yaitu dalam kisaran > 70 % persentase tutupan karang batunya. Dengan demikian diketahui bahwa karang yang berada di kawasan yang dilindungi atau konservasi masih relatif lebih baik kondisinya daripada karang yang berada di kawasan terbuka untuk umum sehingga tekanan kegiatanantropogenik intensitasnya relatif tinggi

Seagrass Community of the Coastal in Southern of Bangka Island, North Minahasa Regancy, North Sulawesi Province

Purpose of this research is to know the community of seagrass, and physical-chemical parameters in the South Bangka island, North Minahasa Regancy, North Sulawesi Province. Data retrieval by the random sampling of systematic method on three lines transect with 50 m long, and 30 quadrants. Data taken in each quadrant is number of species and number of individuals of each species. Results of the measurement of physical and chemical parameters of static waters associated with the feasibility of life for water organisms in it, generally in good condition. Ten species of seagrass have been found in the southern part of the island of Bangka. Four species are always found in any transek i.e Syringodium isoetifolium, Cymodocea rotundata, Enhallus acaroides, and Halophylla ovalis. The highest found in seagrass cover transek 1 i.e. 67.00% while the lowest in transek 3 i.e. 46.30%. Seagrass cover Enhallus acaroides in Bangka island very prominent compared to other species. Based on the current index value is important, Syringodium isoetifolium have a high importance value index only in the transek 3 which means this type of seagrass seagrass types affect other takes part in community level. Ecological index results suggest that the ecosystem of seagrass in Bangka island in the southern part of the State is stable

Site-directed mutagenesis of conserved inverted repeat sequences in the xylanase C promoter region from Streptomyces sp EC3

Streptomyces sp. EC3, a strain which was originally isolated from cattle manure compost, was shown to possess a strong xylanolytic activity. One of the genes responsible for this activity, xlnC, encodes a secreted xylanase. In the native strain, as in the heterologous host S. lividans, expression of xlnC was detectable in the presence of xylan but not in the presence of glucose. Induction by xylan was shown to take place at the transcriptional level. The transcriptional start site of xlnC was mapped and likely -35 (5'-TTGACA-3') and -10 (5'-GAGAAC-3') motifs were identified. In order to localise putative conserved regulatory sequences, the promoter regions of xylanase-encoding genes from various Streptomyces species were aligned. This alignment revealed the existence of three sets of quite well conserved palindromic AT rich sequences called boxes 1, 2 and 3. Box 3 (5'-CGAAA N TTTCG-3') is the farthest away from the promoter region (150-200 bp). A shorter version of this palindrome (5'-GAAA NN TTTC-3') or (5'-CGAAA-3') constitutes box 1, which is located just upstream of the putative -35 promoter sequence. Box 2, located 5-7 bp upstream of box 1, comprises a shorter palindrome than box 3, with inverted polarity [5'-(G/C)TTTC (N) GAAA(G/C)-3']. The putative regulatory role of the conserved inverted repeats in boxes 2 and 3 in the promoter region of the xlnC gene from Streptomyces sp. EC3, was assessed. These boxes were modified by site-directed mutagenesis, and the mutant promoter regions, as well as the wild-type promoter region, were separately fused to a beta-lactamase reporter gene. Analysis of the expression patterns of these fusions in cultures grown in the presence of glucose, xylan or both carbon sources demonstrated that these motifs were cis -acting negative regulatory elements, each playing a specific role in the regulation of xlnC expression. Box 3 was shown to be critical for the establishment of repression of xlnC expression by glucose, whereas box 2 was shown to play an important role in the induction of xlnC expression by xylan.Peer reviewe

Cumulatif valuation of physic-chemical water quality from Tondano Lake, North Sulawesi

The research of  cumulative valuation  of physico-chemical  from Tondano Lake was conducted  in to  dry season on 2015.  The purpose  of the study was to  analysis of water quality of the Lake  such as Total Suspeded Solid (TSS), Total Disolved Solid (TDS), and Conductivity (DHL), as physical  parameters, and also chemical parameters such as  pH, Ca, Mg, K, SO4, Nitrogen Total, NH3, NO3, Phosfat Total, PO4, and Fe.  These were done by taking water sample on 24 location.The data were analysed by multivariate analysis with soft ware statistic R versi 3.3.2, and  Water quality analysis  based on  water quality criteria for  water environment, and Decree of Government Republic Indonesian No.82 /2001 about water quality management and water pollution control.Result revealed that  average and deviation standart  of phyco-chemical  parameters    are   TDS (mg/l)  192.0471 ± 94.1827, TSS (mg/l)  13.33 ± 4.8154 , DHL ( µmhos/cm)  317.833 ± 161.984, pH (6.8875  ± 0.2291), Ca (mg/l) 16.6667  ± 7.2284, Mg (mg/l) 14.7714  ± 0.3860, K (mg/l) 2.330  ± 0.4467, SO4 (mg/l) 5.685  ± 5.3366, Total Nitrogen (mg/l) 2.993  ± 5.3364, NH3 (mg/l) 0.2348  ± 0.8543, NO3 (mg/l) 5.3567  ± 7.0824, Total Phosfat (mg/l) 0.3333  ± 0.4821, PO4 (mg/l) 0.1130  ± 0.3315, Fe (mg/l) 0.11  ± 0.1901Principle Componen Analysis (PCA)  with  Eigen value (cumulative  % of variance)  62.68 %  explaining  10  parameter of physical chemistry water and 10 research location  as  reduction data from initial data.  Cluster analysis with  reduction data formed (1) The research location between Eris village and eceng gondok area, and fish Pond Eris village, the dominant variable were TDS, DHL, Nitrogen Total, and NH3.  (2) The research location in fish Pond Toulimembet  village, in front of  eceng gondok Urongo village, in front of eceng gondok Paleloan village, and in front of eceng gondok Roong village, the dominant variable were SO4, pH, and Mg,  (3) The research location between Kakas villages and eceng gondok area , Kaweng village and eceng gondok area, Kaweng village and eceng gondok area, Toulimembet village and eceng gondok area, and also fish Pond Kaima village,  the dominant variable were TSS, Ca, dan K.Tondano Lake has level of productivity from medium to high, and it is good for fish culture and fishery activity, prossed for drinking water, agriculture, animal husbandry, industry, and water power.Keywords :  Valuation, physical chemistry water, criteria   Abstrak           Penelitian tentang kumulatif kualitas fisik kimia air Danau Tondano telah dilakukan pada  musim kemarau tahun 2015.  Tujuan penelitian adalah (1)  menganalisis kualitas air Danau Tondano  yang meliputi  TSS, TDS, DH sebagai faktor fisika perairan , dan faktor kimia  yaitu derajat keasaman (pH), parameter fisika air danau yaitu Calsium, Magnesium, Kalium, Sulfat, Total nitrogen,  Amoniak, Nitrat, Total Phosfat, Phosfat terlarut, dan Besi (Fe).Sampel air di ambil pada 24 titik pengambilan sampel di Danau Tondano.  Pengambilan sampel air  pada kedalaman 20 meter dilakukan pada 21 titik atau lokasi penelitian,  titik pengambilan sampel pada kedalaman  5 meter, 10 meter, dan 20 meter.Data hasil penelitian dianalisis dengan menggunakan  (1) Analisis multivariate dengan bantuan perangkat lunak statistic R versi R 3.3.2. (2)  Analisis kulaitas air dengan menggunakan kriteria kualitas air bagi lingkungan perairan, dan  Peraturan Pemerintah Republik Indonesia No. 81 Tahun 2001 tentang pengelolaan kualitas air dan pengendalian pencemaran air.Hasil analisis menunjukan konsentrasi  rata-rata dan stadar deviasi TDS (mg/l)  192.0471 ± 94.1827, TSS (mg/l)  13.33 ± 4.8154 , DHL ( µmhos/cm)  317.833 ± 161.984, pH (6.8875  ± 0.2291), Ca (mg/l) 16.6667  ± 7.2284, Mg (mg/l) 14.7714  ± 0.3860, K (mg/l) 2.330  ± 0.4467, SO4 (mg/l) 5.685  ± 5.3366, Total Nitrogen (mg/l) 2.993  ± 5.3364, NH3 (mg/l) 0.2348  ± 0.8543, NO3 (mg/l) 5.3567  ± 7.0824, Total Phosfat (mg/l) 0.3333  ± 0.4821, PO4 (mg/l) 0.1130  ± 0.3315, Fe (mg/l) 0.11  ± 0.1901. Melalui Analisis Komponen Utama (AKU) diperoleh nilai Eigen 62.68 %  yang mampu  menerangkan varian indicator 10 variabel lingkungan dan 10 lokasi penelitian sebagai hasil reduksi data. Analisi kluster dari data reduksi membentuk 3 kluster yaitu  Kluster I di lokasi penelitian belakang eceng gondok Eris dan budidaya ikan Eris dengan variabel  dominan TDS, DHL, Total Nitrogen, dan amoniak.  Kluster II di lokasi penelitian budidaya ikan Toulimembet, depan eceng gondok Urongo, depan gondok Paleloan, Depan eceng gondok Roong dengan variabel dominan  SO4, pH, dan Magnesium.  Kluster III di lokasi penelitian belakang eceng gondok Kakas, belakang eceng gondok Kaweng, belakang eceng gondok Toulimembet, dan budidaya ikan Kaima dengan variabel dominan TSS, Ca, dan K.  Danau Tondano memiliki tingkat kesuburan perairan yang dikategorikan sedang sampai tinggi, dan layak bagi kegiatan perikanan, air danau dapat diolah menjadi air minum, keperluan pertanian, peternakan, perikanan, industri dan listrik tenaga air.Kata kunci: Penilaian, fisika kimia air, Kriteria 

The Critical Role of N- and C-Terminal Contact in Protein Stability and Folding of a Family 10 Xylanase under Extreme Conditions

Stabilization strategies adopted by proteins under extreme conditions are very complex and involve various kinds of interactions. Recent studies have shown that a large proportion of proteins have their N- and C-terminal elements in close contact and suggested they play a role in protein folding and stability. However, the biological significance of this contact remains elusive.In the present study, we investigate the role of N- and C-terminal residue interaction using a family 10 xylanase (BSX) with a TIM-barrel structure that shows stability under high temperature, alkali pH, and protease and SDS treatment. Based on crystal structure, an aromatic cluster was identified that involves Phe4, Trp6 and Tyr343 holding the N- and C-terminus together; this is a unique and important feature of this protein that might be crucial for folding and stability under poly-extreme conditions. folding and activity. Alanine substitution with Phe4, Trp6 and Tyr343 drastically decreased stability under all parameters studied. Importantly, substitution of Phe4 with Trp increased stability in SDS treatment. Mass spectrometry results of limited proteolysis further demonstrated that the Arg344 residue is highly susceptible to trypsin digestion in sensitive mutants such as ΔF4, W6A and Y343A, suggesting again that disruption of the Phe4-Trp6-Tyr343 (F-W-Y) cluster destabilizes the N- and C-terminal interaction. Our results underscore the importance of N- and C-terminal contact through aromatic interactions in protein folding and stability under extreme conditions, and these results may be useful to improve the stability of other proteins under suboptimal conditions

Outcome Prediction in Pneumonia Induced ALI/ARDS by Clinical Features and Peptide Patterns of BALF Determined by Mass Spectrometry

BACKGROUND: Peptide patterns of bronchoalveolar lavage fluid (BALF) were assumed to reflect the complex pathology of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) better than clinical and inflammatory parameters and may be superior for outcome prediction. METHODOLOGY/PRINCIPAL FINDINGS: A training group of patients suffering from ALI/ARDS was compiled from equal numbers of survivors and nonsurvivors. Clinical history, ventilation parameters, Murray's lung injury severity score (Murray's LISS) and interleukins in BALF were gathered. In addition, samples of bronchoalveolar lavage fluid were analyzed by means of hydrophobic chromatography and MALDI-ToF mass spectrometry (MALDI-ToF MS). Receiver operating characteristic (ROC) analysis for each clinical and cytokine parameter revealed interleukin-6>interleukin-8>diabetes mellitus>Murray's LISS as the best outcome predictors. Outcome predicted on the basis of BALF levels of interleukin-6 resulted in 79.4% accuracy, 82.7% sensitivity and 76.1% specificity (area under the ROC curve, AUC, 0.853). Both clinical parameters and cytokines as well as peptide patterns determined by MALDI-ToF MS were analyzed by classification and regression tree (CART) analysis and support vector machine (SVM) algorithms. CART analysis including Murray's LISS, interleukin-6 and interleukin-8 in combination was correct in 78.0%. MALDI-ToF MS of BALF peptides did not reveal a single identifiable biomarker for ARDS. However, classification of patients was successfully achieved based on the entire peptide pattern analyzed using SVM. This method resulted in 90% accuracy, 93.3% sensitivity and 86.7% specificity following a 10-fold cross validation (AUC = 0.953). Subsequent validation of the optimized SVM algorithm with a test group of patients with unknown prognosis yielded 87.5% accuracy, 83.3% sensitivity and 90.0% specificity. CONCLUSIONS/SIGNIFICANCE: MALDI-ToF MS peptide patterns of BALF, evaluated by appropriate mathematical methods can be of value in predicting outcome in pneumonia induced ALI/ARDS

Base-catalysed prototropic rearrangement. Part I. Comparison of the base-catalysed and the metal carbonyl-catalysed isomerisation of allyl ethers

peer reviewedThe light-induced isomerisation of allylic ethers in the presence of pentacarbonyliron has been compared with that brought about by a basic heterogeneous catalyst, KNH2-Al2O3. The reaction on the basic catalyst proceeds through the usual cis-mechanism. In contrast, the carbonyliron reaction gives cis- and trans-isomers, in a 1:1 ratio in the case of allyl phenyl ether. A π-complex was detected (by n.m.r. spectroscopy) as an intermediate in the reaction. The formation of a catalyst which was particularly active for the thermal isomerisation of olefinic double bonds was detected during the irradiation of pentacarbonyliron in allyl phenyl ether. 3-Propoxycyclohexene was readily isomerised under these conditions, whereas it remained unchanged in the presence of very strong bases. Conformational effects of the ring were observed