Intrathoracic impedance and pulmonary wedge pressure for the detection of heart failure deterioration

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Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol

Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA₃Lys:GagPol packaging complex is proposed. Keywords: tRNA₃Lys, lysyl-tRNA synthetase, HIV-1, packaging.Мета. Встановити, чи може N-кінцева послідовність, яка є специфічною для мітохондріальної форми лізил-тРНК синтетази, забезпечувати взаємодію цього ферменту з білком GagPol ВІЛ-1. Методи. Двогібридна дріжджова система, імунопреципітація. Результати. Ми показали що лізил-тРНК синтетаза взаємодіє з доменом Pol білка Gag. Висновки. Запропоновано модель утворення комплексу ЛізРС:тРНК₃Ліз:GagPol. Ключові слова: тРНК₃Ліз, лізил-тРНК синтетаза, ВІЛ-1, збирання віріона.Цель. Выяснить, может ли N-концевая последовательность, являющаяся специфичной для митохондриальной формы лизилтРНК синтетазы, обеспечивать взаимодействие этого фермента с белком GagPol ВИЧ-1. Методы. Двугибридная дрожжевая система, иммунопреципитация Результаты. Мы показали, что лизил-тРНК синтетаза взаимодействует с доменом Pol белка Gag. Выводы. Предложена модель образования комплекса ЛизРС:тРНК₃Лиз:GagPol. Ключевые слова: тРНК₃Лиз, лизил-тРНК синтетаза, ВИЧ-1, сборка вириона

Fine-tuning the electrostatic properties of an alkali-linked organic adlayer on a metal substrate

The performance of modern organic electronic devices is often determined by the electronic level alignment at a metal–organic interface. This property can be controlled by introducing an interfacial electrostatic dipole via the insertion of a stable interlayer between the metallic and the organic phases. Here, we use density functional theory to investigate the electrostatic properties of an assembled structure formed by alkali metals coadsorbed with 7,7,8,8-tetracyanoquinodimethane (TCNQ) molecules on a Ag(100) substrate. We find that the interfacial dipole buildup is regulated by the interplay of adsorption energetics, steric constraints and charge transfer effects, so that choosing chemical substitutions within TCNQ and different alkali metals provides a rich playground to control the systems’ electrostatics and in particular fine-tune its work-function shift

Does the Surface Matter? Hydrogen-Bonded Chain Formation of an Oxalic Amide Derivative in a Two- and Three-Dimensional Environment

We report on a multi-technique investigation of the supramolecular organisation of N,N-diphenyl oxalic amide under differently dimensioned environments, namely three-dimensional (3D) in the bulk crystal, and in two dimensions on the Ag(111) surface as well as on the reconstructed Au(111) surface. With the help of Xray structure analysis and scanning tunneling microscopy (STM) we find that the molecules organize in hydrogen-bonded chains with the bonding motif qualitatively changed by the surface confinement. In two dimensions, the chains exhibit enantiomorphic order even though they consist of a racemic mixture of chiral entities. By a combination of the STM data with near-edge X-ray absorption fine-structure spectroscopy, we show that the conformation of the molecule adapts such that the local registry of the functional group with the substrate is optimized while avoiding steric hindrance of the phenyl groups. In the low coverage case, the length of the chains is limited by the Au(111) reconstruction lines restricting the molecules into fcc stacked areas. A kinetic Monte Carlo simulated annealing is used to explain the selective assembly in the fcc stacked regions

Approaching the socialist factory and its workforce: considerations from fieldwork in (former) Yugoslavia

The socialist factory, as the ‘incubator’ of the new socialist (wo)man, is a productive entry point for the study of socialist modernization and its contradictions. By outlining some theoretical and methodological insights gathered through field-research in factories in former Yugoslavia, we seek to connect the state of labour history in the Balkans to recent breakthroughs made by labour historians of other socialist countries. The first part of this article sketches some of the specificities of the Yugoslav self-managed factory and its heterogeneous workforce. It presents the ambiguous relationship between workers and the factory and demonstrates the variety of life trajectories for workers in Yugoslav state-socialism (from model communists to alienated workers). The second part engages with the available sources for conducting research inside and outside the factory advocating an approach which combines factory and local archives, print media and oral history

The new MMT

ABSTRACT Originally commissioned in 1979, the Multiple Mirror Telescope was a highly innovative and successful facility that pioneered many of the technologies that are used in the new generation of 8 to 10 m class telescopes. After 19 years of operations the MMT was decommissioned in March of 1998: the enclosure was modified, the optics support structure was replaced, and a single 6.5-meter primary mirror was installed and aluminized in-situ. First light for the new MMT was celebrated on May 13, 2000. Operations began with an f/9 optical configuration compatible with existing instruments. Work has continued commissioning two new optical configurations that will serve a suite of new instruments: an f/15 deformable secondary mirror and adaptive optics facility that has obtained diffraction-limited images; and an f/5.4 secondary mirror and refractive corrector that provides a one-degree diameter field of view. The wide-field instrument suite includes two fiber-fed bench spectrographs, a robotic fiber positioner, and a wide-field imaging camera

Health tourism: an opportunity for sustainable development

In February 2017, the “Programma Mattone Internazionale Salute” (ProMis), that is the Italian Program for Internationalization of Regional Health Systems of the Ministry of Health (MoH), presented the first version of its Position Paper on Health Tourism, which embeds a first shared approach to the recommendations expressed by the European Committee of Regions (CoR) on "AgeFriendly" tourism. The CoR stresses the importance of local and regional authorities in the coordination of multi-sectoral policies such as healthcare, social assistance, transport, urban planning and rural development in relation to the promotion of mobility, security, accessibility of services, including health care and social services. "Age-friendly" tourism is an example of an innovative tourist offer that strives to meet the health needs of the entire "traveling" population, with an integrated and cross-sector approach that involves various organizations operating in sectors such as healthcare, accessibility and transport. The aim of the workshop was to explore the interest of the stakeholders to participate in a systemic action in the field of "health" tourism, and to identify priority implementation areas that offer opportunities to take advantage of validated, innovative experiences that strengthen the accessibility to health and social services in regional, national and international contexts. This effort provides the opportunity to take advantage of aligning the European Structural and Investment Funds (ESIF) to the development of tourism, coherently with the needs and resources of local and regional health authorities