Implications of plant material origin, land use history and soil properties in the incidence of verticillium wilt in olive groves

8 páginas, 2 figuras, 1 tabla.The increased presence of Verticillium wilt (Verticillium dahliae Kleb.) in olive groves is often related to the use of infected propagation material and to the planting of new olive trees in contaminated soils. This study assessed the implications of plant propagation, land-use history and soil properties on disease prevalence in southern Spain, the most important olive-growing area worldwide. To this purpose, a large-scale sampling survey was carried out in this area, V. dahliae pathotypes were identified by PCR, and GIS was used to analyze soil properties and cropland-use history. Finally, multiple correspondence analysis was performed to show the statistical association between the variables taken into account. Results strongly indicated the potential risk of planting olive in valleys with irrigated cropland history, especially those that had hosted herbaceous crops, highlighted the importance of using pathogen-free certified planting material as a key component for a successful disease management, and confirmed the role played by saline, alkaline, and steep-slope soils in enhancing V. dahliae prevalence.The research was supported by Caja Rural Foundation.Peer reviewe

Scenarios of intermittent E. coli contamination from sewer overflows to shellfish growing waters: the Dart Estuary case study

Sewage overflows (SOs) and Combined Sewer Overflows (CSOs) significantly contribute to the bacterial contamination of coastal waters, which is of especial concern for aquaculture, a growing industry worldwide. Hydrodynamic and water quality models were used to investigate impacts of CSO discharge frequency and duration, river discharge and tides on Escherichia coli levels at shellfish farming sites in the Dart Estuary (UK), being the employed methodology generally applicable. High E. coli contamination occurred during neap tides and high river discharges due to higher retention and lower bacterial decay. Synchronicity of CSO spills affected the duration of the pollution episodes rather than peak concentrations, more influenced by discharges of the neighbouring CSOs. During peak discharges, E. coli concentrations could be 10 times higher than during average flows. CSO spills were more frequent when rainfall was >20 mm. Model outputs combined with rainfall forecasts can indicate microbiological contamination risk in the aquaculture sites.En prensa2,35

S wave velocity structure below central Mexico using high-resolution surface wave tomography

Shear wave velocity of the crust below central Mexico is estimated using surface wave dispersion measurements from regional earthquakes recorded on a dense, 500 km long linear seismic network. Vertical components of regional records from 90 well-located earthquakes were used to compute Rayleigh-wave group-velocity dispersion curves. A tomographic inversion, with high resolution in a zone close to the array, obtained for periods between 5 and 50 s reveals significant differences relative to a reference model, especially at larger periods (>30 s). A 2-D S wave velocity model is obtained from the inversion of local dispersion curves that were reconstructed from the tomographic solutions. The results show large differences, especially in the lower crust, among back-arc, volcanic arc, and fore-arc regions; they also show a well-resolved low-velocity zone just below the active part of the Trans Mexican Volcanic Belt (TMVB) suggesting the presence of a mantle wedge. Low densities in the back arc, inferred from the low shear wave velocities, can provide isostatic support for the TMVB

A new strategy to design SIW-fed arrays

In this work, a new full-wave strategy, with very low iteration times, is proposed for the optimization and design of antenna arrays fed by substrate integrated waveguides. The device is decomposed into fixed and modifiable (to be optimized) sections, whose generalized scattering matrices are precomputed. The response of the array is calculated by considering firstly only the interactions between the fixed sections, which are then coupled to the modifiable ones in each iteration of an optimization process. To validate the proposed strategy, a transversal 16-slot antenna array, placed on the top plate of a substrate integrated waveguide, has been designed. A speed-up factor of over 2000 times, compared to general purpose commercial software, has been obtained in this optimization process. The final design presents a 1.05 GHz bandwidth under - 10 dB in terms of |S11|, a maximum realized gain of 17.5 dBi at 17 GHz, and a 99.95% maximum efficiency (without dielectric and conductor losses). Index Terms—Addition theorems, cylindrical modes, spherical modes, optimization process, antenna array, substrate integrated waveguide (SIW)

A Cytochemical Scanning Electron Microscopy Study of Non-Specific Acid Esterase and Acid Phosphatase Activities in Human Peripheral Blood Lymphocytes

We analyzed the distribution patterns of nonspecific acid esterase and acid phosphatase activities with cytochemistry-scanning electron microscopy in backscattered and secondary electron imaging modes in isolated normal human peripheral blood lymphocytes . The analysis of non-specific acid esterase activity in the backscattered electron imaging mode showed, in some cells, focal distribution with a well-defined, homogenous deposit. Two patterns of acid phosphatase activity were evident with the backscattered electron imaging mode, i.e., focal and granular. Peripheral blood lymphocytes showing focal activity of both enzymes presented a smooth surface with few scattered microvilli as seen with the secondary electron imaging mode ; while lymphocytes with a granular pattern of acid phosphatase activity had abundant microvilli . The correlation between patterns of enzymatic activity as seen in backscattering electron imaging mode, and surface morphological features as seen with secondary electron imaging mode, distinguished a subpopulation of lymphocytes of T lineage in human peripheral blood

Temperature-independent quantum logic for molecular spectroscopy

We propose a fast and non-destructive spectroscopic method for single molecular ions that implements quantum logic schemes between an atomic ion and the molecular ion of interest. Our proposal relies on a hybrid coherent manipulation of the two-ion system, using optical or magnetic forces depending on the types of molecular levels to be addressed (Zeeman, rotational, vibrational or electronic degrees of freedom). The method is especially suited for the non-destructive precision spectroscopy of single molecular ions, and sets a starting point for new hybrid quantum computation schemes that combine molecular and atomic ions, covering the measurement and entangling steps.Comment: v3. Substantially enlarged manuscript with details of derivations and calculations in two appendices. To appear in PR

Noninvasive assessment of inspiratory muscle neuromechanical coupling during inspiratory threshold loading

Diaphragm neuromechanical coupling (NMC), which reflects the efficiency of conversion of neural activation to transdiaphragmatic pressure (Pdi), is increasingly recognized to be a useful clinical index of diaphragm function and respiratory mechanics in neuromuscular weakness and cardiorespiratory disease. However, the current gold standard assessment of diaphragm NMC requires invasive measurements of Pdi and crural diaphragm electromyography (oesEMGdi), which complicates the measurement of diaphragm NMC in clinical practice. This is the first study to compare invasive measurements of diaphragm NMC (iNMC) using the relationship between Pdi and oesEMGdi, with noninvasive assessment of NMC (nNMC) using surface mechanomyography (sMMGlic) and electromyography (sEMGlic) of lower chest wall inspiratory muscles. Both invasive and noninvasive measurements were recorded in twelve healthy adult subjects during an inspiratory threshold loading protocol. A linear relationship between noninvasive sMMGlic and sEMGlic measurements was found, resulting in little change in nNMC with increasing inspiratory load. By contrast, a curvilinear relationship between invasive Pdi and oesEMGdi measurements was observed, such that there was a progressive increase in iNMC with increasing inspiratory threshold load. Progressive recruitment of lower ribcage muscles, serving to enhance the mechanical advantage of the diaphragm, may explain the more linear relationship between sMMGlic and sEMGlic (both representing lower intercostal plus costal diaphragm activity) than between Pdi and crural oesEMGdi. Noninvasive indices of NMC derived from sEMGlic and sMMGlic may prove to be useful indices of lower chest wall inspiratory muscle NMC, particularly in settings that do not have access to invasive measures of diaphragm function.Peer ReviewedPostprint (published version

Influencia del ángulo de la proyección de abrasivos en la limpieza de materiales pétreos detríticos en Patrimonio Arquitectónico

In this research, the influence of the angle in abrasive blasting cleaning is studied on Montjuïc sandstone with black crust. After analyzing the properties of the soiling and the material, and their possible influence on the treatment, different cleaning tests were made at four different angles, keeping the complementary parameters constant. Taking the restorer’s perspective as a starting point, and in order to fulfill the practical requirements of an intervention —time and cost reduction—, tests were evaluated with USB digital microscope, stereomicroscope with 3D visualization and measurement, and colorimeter. From the results it is established that angles close to 75° minimize surface alteration, reducing differential erosion in the binding phases of detritic materials usually caused by this treatment.En este trabajo se estudia la influencia del ángulo de la proyección de abrasivos en la limpieza de una arenisca de Montjuïc con costra negra. Tras analizar las propiedades del material, de la suciedad y su posible influencia en el tratamiento, se realizan diferentes catas de limpieza con cuatro ángulos distintos manteniendo constantes el resto de parámetros de la proyección. Partiendo de la visión del conservador-restaurador y de un carácter práctico según las necesidades reales de una intervención —reducción de tiempos y costes—, los ensayos se evalúan con microscopio digital USB, microscopio estereoscópico con visualización y medición en 3D y colorímetro. De los resultados se puede determinar que ángulos cercanos a 75° minimizan la alteración de la superficie al reducir la erosión diferencial de las fases de unión que el tratamiento normalmente provoca en los materiales detríticos

The Influence of Host Fruit and Temperature on the Body Size of Adult Ceratitis capitata (Diptera: Tephritidae) under Laboratory and Field conditions

The adult body size of the Mediterranean fruit ßy, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), varies in natural conditions. Body size is an important Þtness indicator in the Mediterranean fruit ßy;largerindividuals are more competitive at mating and have a greater dispersion capacity and fertility. Both temperature during larval development and host fruit quality have been cited as possible causes for this variation.We studied the inßuence of host fruit and temperature during larval development on adult body size (wing area) in the laboratory, and determined body size variation in Þeld populations of the Mediterannean fruit ßy in eastern Spain. Field ßies measured had two origins: 1) ßies periodically collected throughout the year in Þeld traps from 32 citrus groves, during the period 2003Ð2007; and 2) ßies evolved from different fruit species collected between June and December in 2003 and 2004. In the lab, wing area of male and female adults varied signiÞcantly with temperature during larval development, being larger at the lowest temperature. Adult size also was signiÞcantly different depending on the host fruit in which larvae developed. The size of the ßies captured at the Þeld, either from traps or from fruits, varied seasonally showing a gradual pattern of change along the year. The largest individuals were obtained during winter and early spring and the smallest during late summer. In Þeld conditions, the size of the adult Mediterannean fruit ßy seems apparently more related with air temperature than with host fruit. The implications of this adult size pattern on the biology ofC. capitata and on the application of the sterile insect technique are discussed.We thank Apostolos Pekas for his useful comments on previous versions of the manuscript. This work was supported by the project RTA03-103-C6-3 assigned to F. G. M. from the Ministerio de Educacion y Ciencia of Spain.Navarro Campos, C.; Martínez Ferrer, MT.; Campos, J.; Fibla, JM.; Alcaide, J.; Bargues Desolmes, L.; Marzal Moreno, C.... (2011). The Influence of Host Fruit and Temperature on the Body Size of Adult Ceratitis capitata (Diptera: Tephritidae) under Laboratory and Field conditions. Environmental Entomology. 90(4):931-938. https://doi.org/10.1603/EN10302S931938904Albajes R. Santiago-Alvarez C. 1980. Influencia de la temperatura en el desarrollo de Ceratitis capitata (Diptera: Trypetidae). An. INIA. 13: 183–190.Angilletta, Jr.,, M. J., & Dunham, A. E. (2003). The Temperature‐Size Rule in Ectotherms: Simple Evolutionary Explanations May Not Be General. The American Naturalist, 162(3), 332-342. doi:10.1086/377187Arita L.H. Kaneshiro K.Y. 1988. Body size and differential mating success between males of two populations of the Mediterranean fruit fly. Pac. Sci. 42: 173–177.Atkinson D. 1994. Temperature and organism size: a biological law for ectotherms?. Adv. Ecol. Res. 25: 1–58.Atkinson, D., & Sibly, R. M. (1997). Why are organisms usually bigger in colder environments? Making sense of a life history puzzle. Trends in Ecology & Evolution, 12(6), 235-239. doi:10.1016/s0169-5347(97)01058-6Back E.A. Pemberton C.E. 1918. The mediterranean fruit fly. U.S. Dep. Agric. Bull. 640: 1–43.BLAY, S., & YUVAL, B. (1997). Nutritional correlates of reproductive success of male Mediterranean fruit flies (Diptera: Tephritidae). Animal Behaviour, 54(1), 59-66. doi:10.1006/anbe.1996.0445Bodenheimer F.S. 1951. Citrus entomology in the Middle East. W. Junk. The Hague, Netherlands. 1–663.Calkins C.O. 1984. The importance of understanding fruit fly mating behavior in sterile male release programs (Diptera: Tephritidae). Folia Entomol. Mexicana. 61: 205–213.Carey J.R. 1984. Host-specific demographic studies of the Mediterranean fruit fly, Ceratitis capitata . Ecol. Entomol. 9: 261–270.Chapman R.F. 1998. The insects: structure and function. 4th ed. Cambridge University Press, Cambridge, United Kingdom.Christenson, L. D., & Foote, R. H. (1960). Biology of Fruit Flies. Annual Review of Entomology, 5(1), 171-192. doi:10.1146/annurev.en.05.010160.001131Churchill-Stanland, C., Stanland, R., Wong, T. T. Y., Tanaka, N., McInnis, D. O., & Dowell, R. V. (1986). Size as a Factor in the Mating Propensity of Mediterranean Fruit Flies, Ceratitis capitata (Diptera: Tephritidae), in the Laboratory. Journal of Economic Entomology, 79(3), 614-619. doi:10.1093/jee/79.3.614Danthanarayana W. 1976. Environmentally cued size variation in the light-brown apple moth, Epiphyas postvittana (Walk.) (Tortricidae), and its adaptive value in dispersal. Oecologia. 26: 121–132.Davidowitz G. Nijhout H.F. 2004. The physiological basis of reaction norms: the interaction among growth rate, the duration of growth and body size. Integr. Comp. Biol. 144: 443–449.Davidowitz G.L. D'Amico J. Nijhout H.F. 2004. The effects of environmental variation on a mechanism that controls insect body size. Evol. Ecol. Res. 6: 49–62.Debouzie D. 1977. Etude de la competition larvaire chez Ceratitis capitata (Dyptère, Trypetidae). Arch. Zool. Exp. Gen. 118: 315–334.Diamond, S. E., & Kingsolver, J. G. (2010). Environmental Dependence of Thermal Reaction Norms: Host Plant Quality Can Reverse the Temperature‐Size Rule. The American Naturalist, 175(1), 1-10. doi:10.1086/648602Eberhard W. 2000. Sexual behavior and sexual selection in the Mediterranean fruit fly, Ceratitis capitata (Dacinae: Ceratitidini) In . Aluja M. Norrbom A. Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC, Boca Raton, FL.Edgar, B. A. (2006). How flies get their size: genetics meets physiology. Nature Reviews Genetics, 7(12), 907-916. doi:10.1038/nrg1989Fletcher B.S. 1989a. Movements of tephritid fruit flies, pp. 209–219 In . Robinson A.S. Hooper G. World crop pests, vol. 3B. Fruits flies, their biology, natural enemies and control. Elsevier, Amsterdam.Fletcher B.S. 1989b. Life History Strategies of Tephritid fruit flies, pp. 195–208 In . Robinson A.S. Hooper G. World crop pests, vol. 3B. Fruits flies, their biology, natural enemies and control. Elsevier, Amsterdam.Gilchrist A.S. Crisafulli D.C. 2006. Using variation in wing shape to distinguish between wild and mass-reared individuals of Queensland fruit fly, Bactrocera tryoni . Entomol. Exp. Appl. 119: 175–178.Gilchrist A.S. Partridge L. 2001. The contrasting genetic architecture of wing size and shape in Drosophila melanogaster . Heredity. 86: 144–152.Gómez Clemente F. Planes S. 1952. Algunas notas sobre la ecología de Ceratitis capitata en el Levante español sobre naranjos. Bol. Patol. Veg. Entomol. Agric. 19: 37–48.Hasson O. Rossler Y. 2002. Character-specific homeostasis dominates fluctuating asymmetries in the medfly (Diptera: Tephritidae). Fla. Entomol. 85: 73–82.HOFFMANN, A. A., RATNA, E., SGRÒ, C. M., BARTON, M., BLACKET, M., HALLAS, R., … WEEKS, A. R. (2007). Antagonistic selection between adult thorax and wing size in field released Drosophila melanogaster independent of thermal conditions. Journal of Evolutionary Biology, 20(6), 2219-2227. doi:10.1111/j.1420-9101.2007.01422.xInglesfield C. 1982. Larval hosts, adult body size and population quality in Ceratitis capitata Wied.: a laboratory study. Annali della Facoltà di Agraria dell'Università di Sassari. 28: 25–39.Israely, N., Yuval, B., Kitron, U., & Nestel, D. (1997). Population Fluctuations of Adult Mediterranean Fruit Flies (Diptera: Tephritidae) in a Mediterranean Heterogeneous Agricultural Region. Environmental Entomology, 26(6), 1263-1269. doi:10.1093/ee/26.6.1263Joaquim-Bravo I.S. Guimaraes A.N. Magalhaes T.C. Nascimento A.S. 2010. Performance of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) in fruits: comparison of two laboratory populations. Neotrop. Entomol. 39: 9–14.Kaspi, R., Taylor, P. W., & Yuval, B. (2000). Diet and size influence sexual advertisement and copulatory success of males in Mediterranean fruit fly leks. Ecological Entomology, 25(3), 279-284. doi:10.1046/j.1365-2311.2000.00266.xKaspi R. Mossinson S. Drezner T. Kamensky B. Yuval B. 2002. Effects of larval diet on development rates and reproductive maturation of male and female Mediterranean fruit flies. Physiol. Entomol. 27: 29–38.Kingsolver J.G. Shlichta J.G. Ragland G.J. Massie K.R. 2006. Thermal reaction norms for caterpillar growth depend on diet. Evol. Ecol. Res. 8: 703–715.Krainacker, D. A., Carey, J. R., & Vargas, R. I. (1987). Effect of larval host on life history traits of the mediterranean fruit fly, Ceratitis capitata. Oecologia, 73(4), 583-590. doi:10.1007/bf00379420Krainacker, D. A., Carey, J. R., & Vargas, R. I. (1989). Size-Specific Survival and Fecundity for Laboratory Strains of Two Tephritid (Diptera: Tephritidae) Species: Implications for Mass Rearing. Journal of Economic Entomology, 82(1), 104-108. doi:10.1093/jee/82.1.104Liquido N.J. Shinoda L.A. Cunningham R.T. 1991. Host plants of Mediterranean fruit fly: an annotated world review. Ann. Entomol. Soc. Am. 77: 1–52.Martínez-Ferrer M.T. Campos J.M. Fibla J.M. 2006. Population dynamics of Ceratitis capitata on citrus in northeastern Spain: influence of adjacent host fruit trees. IOBC-WPRS Bull. 29: 77–84.Martínez-Ferrer M.T. Navarro C. Campos J.M. Marzal C. Fibla J.M. Bargues L. Garcia-Mari F. 2010. Seasonal and annual trends in field populations of Mediterranean fruit fly, Ceratitis capitata, in Mediterranean citrus groves: comparison of two geographic areas in eastern Spain. Spanish. J. Agric. Res. 8: 757–765.Weitzman, J. (2006). Journal of Biology, 5(1), 1. doi:10.1186/jbiol33PAPADOPOULOS, N. T., CAREY, J. R., LIEDO, P., MÜLLER, H.-G., & SENTÜRK, D. (2009). Virgin females compete for mates in the male lekking speciesCeratitis capitata. Physiological Entomology, 34(3), 238-245. doi:10.1111/j.1365-3032.2009.00680.xProkopy, R. J., & Hendrichs, J. (1979). Mating Behavior of Ceratitis capitata1 on a Field-Caged Host Tree. Annals of the Entomological Society of America, 72(5), 642-648. doi:10.1093/aesa/72.5.642Ray, C. (1960). The application of Bergmann’s and Allen’s rules to the poikilotherms. Journal of Morphology, 106(1), 85-108. doi:10.1002/jmor.1051060104Rivnay E. 1950. The Mediterranean fruit fly in Israel. Bull. Entomol. Res. 41: 321–341.Sankarperumal, G., & Pandian, T. J. (1991). Effect of temperature andChlorelladensity on growth and metamorphosis ofChironomus circumdatus(Kieffer) (Diptera). Aquatic Insects, 13(3), 167-177. doi:10.1080/01650429109361438Santaballa E. Laborda R. Bargues L. 2001. Tratamientos de cuarentena: evolución y supervivencia de la mosca de las frutas Ceratitis capitata (Wiedemann) sobre cítricos. Levante Agric. 358: 405–412.Sharp, J. L., Boller, E. F., & Chambers, D. L. (1983). Selection for Flight Propensity of Laboratory and Wild Strains of Anastrepha suspensa and Ceratitis capitata (Diptera: Tephritidae)1. Journal of Economic Entomology, 76(2), 302-305. doi:10.1093/jee/76.2.302Sigurjonsdottir H. 1984. Food competition among Scatophaga stercoraria larvae with emphasis on its effects on reproductive success. Ecol. Entomol. 9: 81–90.Sivinski J. Aluja M. Dodson G.N. Freidberg A. Headrick D.H. Kaneshiro K.Y. Landolt P. 2000. Topics in the evolution of sexual behavior in the Tephritidae, pp. 751–792 In . Aluja M. Norrbom A. Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC, Boca Raton, FL.Stamp, N. E. (1990). Growth versus molting time of caterpillars as a function of temperature, nutrient concentration and the phenolic rutin. Oecologia, 82(1), 107-113. doi:10.1007/bf00318541Statgraphics. 1994. Version 5.1 Plus. Statistical graphics system by Statistical Graphics Corporation, Manugistics, Rockville, MD.Torres-Vila L.M. Sanchez Á. Ponce F. Delgado E. Aza M.C. Barrena F. Ferrero J.J. Cruces E. Rodriguez F. 2006. Dinámica poblacional de Bractocera oleae Gmelin en Extremadura: fluctuación estacional en el estado reproductivo y en el tamaño inaginal. Bol. Sanid. Veg., Plagas. 32: 57–69.Whittier, T. S., Nam, F. Y., Shelly, T. E., & Kaneshiro, K. Y. (1994). Male courtship success and female discrimination in the mediterranean fruit fly (Diptera: Tephritidae). Journal of Insect Behavior, 7(2), 159-170. doi:10.1007/bf01990078Yuval, B., Wekesa, J. W., Lemenager, D., Kauffman, E. E., & Washino, R. K. (1993). Seasonal Variation in Body Size of Mosquitoes (Diptera: Culicidae) in a Rice Culture Agroecosystem. Environmental Entomology, 22(2), 459-463. doi:10.1093/ee/22.2.459Zucoloto F.S. 1987. Feeding habits of Ceratitis capitata (Diptera: Tephritidae): can larvae recognize a nutritionally effective diet?. J. Insect Physiol. 33: 349–353