Compaction caused by soil tillage and seedbed preparation: effects on weed emergence in a barley crop

El objetivo principal de este trabajo fue evaluar los efectos de la compactación producida por la frecuencia de pases y el tipo de apero utilizado en la siembra del cereal, sobre la emergencia de las malas hierbas. Se utilizó un diseño en parcelas subdivididas con 4 repeticiones, con la labor primaria (fresa vs. cultivador) en parcelas principales y el pase de rulo (liso vs. pratenses) en subparcelas. Se tomaron datos de malas hierbas (densidad, biomasa) y de propiedades físicas del suelo (compactación, densidad aparente). Los resultados no han mostrado diferencias debidas a la labor primaria en ninguna de las variables analizadas, pero sí en función del tipo y frecuencia de pases de rulo, observándose menor densidad y biomasa de malas hierbas cuanto mayor era el número de pases de rulo, especialmente el liso, coincidiendo con los suelos más compactados. Sin embargo, parece existir un límite en el cual dejan de producirse efectos significativos debido a una sobrecompactación (e.g. doble pase de rulo y zona de rodada).The main objective of this work was to evaluate the effects of the compaction produced by the frequency of passes and the type of implement used in seedbed preparation, on weed emergence. A split-plot design with 4 replications was used, with the primary tillage (cultivator vs. rotary tiller) in main plots and rolling (flat roller vs. Cambridge roller) in subplots. Weeds data (density, biomass) and soil physical properties (compaction, bulk density) were assessed. The results showed no differences due to the primary tillage in any of the analyzed variables. However, the type and frequency of roller passes resulted in significant differences in weed abundance, with lower weed density and biomass when the number of roller passes was greater (especially flat roller), which coincided with the higher compaction. However, it seems to be a limit at which significant effects no longer occur due to an overcompaction (e.g. double roller pass and tractor wheel traffic)

Effect of wheel track on the density and composition of weeds in a maize crop

El objetivo de este estudio fue analizar si la compactación producida por la rodada del tractor durante las operaciones de siembra influye en la composición y densidad de malas hierbas en cultivo de maíz. Para ello se llevó a cabo el conteo e identificación de las especies presentes en 160 unidades muestrales localizadas en la interlínea de cultivo, de las cuales la mitad estaba afectada por la rodada del tractor y la otra mitad no. El contraste de medias entre los datos con rodada y sin rodada para la riqueza de especies, densidad total y densidad por especies se realizó mediante el test de Mann-Whitney. Los resultados han puesto de manifiesto que las especies principales, a excepción de “Cyperus rotundus”, fueron significativamente más abundantes en las interlíneas con rodada. En relación a la comunidad arvense, ésta fue igualmente más diversa y abundante en las interlíneas con rodada.The aim of this study was to analyze whether the compaction caused by the tractor during sowing operations affects the composition and density of the weed flora in maize crops. For that, 160 sample units were taken in the crop interline, where half of them were affected by the tractor tread and the other half not. In each sampling unit we carried out the count and identification of weed species. The Mann-Whitney test was performed to contrast differences in species richness, total and individual species densities between tractor-tread and not tractor-tread data. The results revealed that the main species, except for “Cyperus rotundus” were significantly more abundant in the interline with tractor tread. In relation to the weed community, it was also more diverse and abundant in the interline with tractor tread

Accompanying weeds of a poplar energy crop under different management strategies

Se han evaluado los efectos de distintas estrategias de manejo sobre las poblaciones de malas hierbas y la productividad (i.e. biomasa) de un cultivo energético de chopo en la zona centro de España. Para ello, se han realizado dos estudios: 1) análisis durante el primer año de cultivo, el más sensible a la competencia con arvenses, repetido tres veces; y 2) valoración al cabo de los tres años de duración del turno de corte. Los resultados han puesto de manifiesto una evolución de la flora arvense con el tiempo, encontrando cambios significativos según la estrategia de manejo. Al finalizar el turno de tres años, las estrategias más productivas fueron la cubierta vegetal sembrada con “Lolium multiflorum” y la basada en herbicidas, coincidiendo con una menor competencia de malas hierbas respecto a la estrategia estándar basada en labores.We have evaluated the effects of different management strategies on weed populations and productivity (i.e. biomass) of a poplar energy crop in central Spain. To do this, two studies have been performed: 1) analysis performed during the first year of production, the most sensitive to competition with weeds, repeated three times; and 2) an assessment after the three-year harvest cycle. The results have revealed a weed flora evolution over time, finding significant changes as a function of management strategies. At the end of the three-year harvest cycle, the most productive strategies were cover crop sown with “Lolium multiflorum” and that based on herbicides, coinciding with less competition with weeds compared to the standard strategy based on tillage

3D reconstruction of weeds using depth cameras

El objetivo de este estudio fue optimizar el ángulo de posicionamiento del sensor Kinect para la reconstrucción de la estructura tridimensional de Xanthium strumarium L., Datura stramonium L. y Chenopodium album L., utilizando para ello algoritmos que permiten la captura y combinación de imágenes de profundidad y RGB. Se han comparado diferentes ángulos, fijando en cada uno de ellos el sensor Kinect de forma estática respecto de la planta objetivo. Los resultados han confirmado la correlación entre la biomasa de malas hierbas y el área estimada con el sensor. La estimación de la altura de las plantas también fue adecuada, con una media de 2cm de error dependiendo de la posición del sensor. Sin embargo, aunque el sensor ha mostrado su capacidad para la creación de modelos tridimensionales, el adecuado posicionamiento del sensor es fundamental para la correcta reconstrucción de plantas. La posición ideal del sensor debe ser elegida de acuerdo a la especie a medir y su estado fenológico. Estos resultados sugieren que Kinect es una herramienta útil para caracterizar de forma rápida y fiable las malas hierbas, con importantes ventajas sobre otros sensores debido a su bajo coste, bajo requerimiento energético y alta frecuencia de transmisión de imágenes.The objective of this study was to optimize the positioning angle of a Kinect sensor for reconstructing the three dimensional structure of weeds, using Kinect fusion algorithms to generate a 3D point cloud from the depth video stream. The sensor was mounted in different positions facing the plant in order to obtain depth (RGB-D) images from different angles. The results confirmed the correlation between ground truth (e. g. weed biomass) and the measured area with Kinect. In addition, plant height was accurately estimated with a few centimeters error. However, although the Kinect sensor has shown its ability for plant reconstruction, proper positioning of the sensor is critical for correct reconstruction of plants. The best position of the sensor must be chosen according to the species to be measured and their growth stage. These results suggest that Kinect is a promising tool for a rapid and reliable weed characterization, with several important advantages such as low cost, low power requirement and a high frame rate

Enzyme-Mediated Quenching of the Pseudomonas Quinolone Signal (PQS):A Comparison between Naturally Occurring and Engineered PQS-Cleaving Dioxygenases

The opportunistic pathogen Pseudomonas aeruginosa employs quorum sensing to govern the production of many virulence factors. Interference with quorum sensing signaling has therefore been put forward as an attractive approach to disarm this pathogen. Here, we analyzed the quorum quenching properties of natural and engineered (2-alkyl-)3-hydroxy-4(1H)-quinolone 2,4-dioxygenases (HQDs) that inactivate the P. aeruginosa signal molecule PQS (Pseudomonas quinolone signal; 2-heptyl-3-hydroxy-4(1H)-quinolone). When added exogenously to P. aeruginosa cultures, all HQDs tested significantly reduced the levels of PQS and other alkylquinolone-type secondary metabolites deriving from the biosynthetic pathway, such as the respiratory inhibitor 2-heptyl-4-hydroxyquinoline N-oxide. HQDs from Nocardia farcinica and Streptomyces bingchenggensis, which combine low K(M) values for PQS with thermal stability and resilience in the presence of P. aeruginosa exoproducts, respectively, attenuated production of the virulence factors pyocyanin and pyoverdine. A delay in mortality was observed when Galleria mellonella larvae were infected with P. aeruginosa suspensions treated with the S. bingchenggensis HQD or with inhibitors of alkylquinolone biosynthesis. Our data indicate that quenching of PQS signaling has potential as an anti-virulence strategy; however, an efficient anti-virulence therapy against P. aeruginosa likely requires a combination of agents addressing multiple targets

Monitoring dynamics of human adenovirus disassembly induced by mechanical fatigue

The standard pathway for virus infection of eukaryotic cells requires disassembly of the viral shell to facilitate release of the viral genome into the host cell. Here we use mechanical fatigue, well below rupture strength, to induce stepwise disruption of individual human adenovirus particles under physiological conditions, and simultaneously monitor disassembly in real time. Our data show the sequence of dismantling events in individual mature (infectious) and immature (noninfectious) virions, starting with consecutive release of vertex structures followed by capsid cracking and core exposure. Further, our experiments demonstrate that vertex resilience depends inextricably on maturation, and establish the relevance of penton vacancies as seeding loci for virus shell disruption. The mechanical fatigue disruption route recapitulates the adenovirus disassembly pathway in vivo, as well as the stability differences between mature and immature virionsWe acknowledge funding by grants from the Ministry of Science and Innovation of Spain, PIB2010US-00233, FIS2011-29493, Consolider CSD2010-00024 and CAM project and the Comunidad de Madrid No. S2009/MAT-1467 to P. J. P.; BFU2010-16382/BMC to C.S.M.; and FIS2011-16090-E to C.S.M. and P.J.P. S.J.F acknowledges funding from the National Institutes of Health, USA (GM037705 and AI1058172). A.J.P.-B. holds a Juan de la Cierva postdoctoral contract from the Ministry of Science and Innovation of Spain; A.O.-E. and R.M.-C. are recipients of predoctoral fellowships from the Ministry of Education and the Instituto de Salud Carlos III of Spain, respectivel

Resonant radiation pressure on neutral particles in a waveguide

A theoretical analysis of electromagnetic forces on neutral particles in an hollow waveguide is presented. We show that the effective scattering cross section of a very small (Rayleigh) particle can be strongly modified inside a waveguide. The coupling of the scattered dipolar field with the waveguide modes induce a resonant enhanced backscattering state of the scatterer-guide system close to the onset of new modes. The particle effective cross section can then be as large as the wavelength even far from any transition resonance. As we will show, a small particle can be strongly accelerated along the guide axis while being highly confined in a narrow zone of the cross section of the guide.Comment: RevTeX,4 pages,3 PS figure

Magnetic phase evolution in the spinel compounds Zn1−x_{1-x}Cox_xCr2_2O4_4

We present the magnetic properties of complete solid solutions of ZnCr$_2$O$_4$ and CoCr$_2$O$_4$: two well-studied oxide spinels with very different magnetic ground states. ZnCr$_2$O$_4$, with non-magnetic $d^{10}$ cations occupying the A site and magnetic $d^3$ cations on the B site, is a highly frustrated antiferromagnet. CoCr$_2$O$_4$, with magnetic $d^7$ cations (three unpaired electrons) on the A site as well, exhibits both N\'eel ferrimagnetism as well as commensurate and incommensurate non-collinear magnetic order. More recently, CoCr$_2$O$_4$ has been studied extensively for its polar behavior which arises from conical magnetic ordering. Gradually introducing magnetism on the A site of ZnCr$_2$O$_4$ results in a transition from frustrated antiferromagnetism to glassy magnetism at low concentrations of Co, and eventually to ferrimagnetic and conical ground states at higher concentrations. Real-space Monte-Carlo simulations of the magnetic susceptibility suggest that the first magnetic ordering transition and features of the susceptibility across $x$ are captured by near-neighbor self- and cross-couplings between the magnetic A and B atoms. We present as a part of this study, a method for displaying the temperature dependence of magnetic susceptibility in a manner which helps distinguish between compounds possessing purely antiferromagnetic interactions from compounds where other kinds of ordering are present.Comment: 10 pages, 5 figures, 1 tabl