2,678 research outputs found
automRm: An R Package for Fully Automatic LC-QQQ-MS Data Preprocessing Powered by Machine Learning
Preprocessing of liquid chromatography-mass spectrometry (LC-MS) raw data facilitates downstream statistical and biological data analyses. In the case of targeted LC-MS data, consistent recognition of chromatographic peaks is a main challenge, in particular, for low abundant signals. Fully automatic preprocessing is faster than manual peak review and does not depend on the individual operator. Here, we present the R package automRm for fully automatic preprocessing of LC-MS data recorded in MRM mode. Using machine learning (ML) for detection of chromatographic peaks and quality control of reported results enables the automatic recognition of complex patterns in raw data. In addition, this approach renders automRm generally applicable to a wide range of analytical methods including hydrophilic interaction liquid chromatography (HILIC), which is known for sample-to-sample variations in peak shape and retention time. We demonstrate the impact of the choice of training data set, of the applied ML algorithm, and of individual peak characteristics on automRm’s ability to correctly report chromatographic peaks. Next, we show that automRm can replicate results obtained by manual peak review on published data. Moreover, automRm outperforms alternative software solutions regarding the variation in peak integration among replicate measurements and the number of correctly reported peaks when applied to a HILIC-MS data set. The R package is freely available from gitlab (https://gitlab.gwdg.de/joerg.buescher/automrm)
Targeted LC-MS/MS-based metabolomics and lipidomics on limited hematopoietic stem cell numbers
Metabolism is important for the regulation of hematopoietic stem cells (HSCs) and drives cellular fate. Due to the scarcity of HSCs, it has been technically challenging to perform metabolome analyses gaining insight into HSC metabolic regulatory networks. Here, we present two targeted liquid chromatography–mass spectrometry approaches that enable the detection of metabolites after fluorescence-activated cell sorting when sample amounts are limited. One protocol covers signaling lipids and retinoids, while the second detects tricarboxylic acid cycle metabolites and amino acids. For complete details on the use and execution of this protocol, please refer to Schönberger et al. (2022)
Polarized Proton Beams from Laser-induced Plasmas
We report on the concept of an innovative source to produce polarized
proton/deuteron beams of a kinetic energy up to several GeV from a laser-driven
plasma accelerator. Spin effects have been implemented into the PIC simulation
code VLPL to make theoretical predictions about the behavior of proton spins in
laser-induced plasmas. Simulations of spin-polarized targets show that the
polarization is conserved during the acceleration process. For the experimental
realization, a polarized HCl gas-jet target is under construction using the
fundamental wavelength of a Nd:YAG laser system to align the HCl bonds and
simultaneously circular polarized light of the fifth harmonic to
photo-dissociate, yielding nuclear polarized H atoms. Subsequently, their
degree of polarization is measured with a Lamb-shift polarimeter. The final
experiments, aiming at the first observation of a polarized particle beam from
laser-generated plasmas, will be carried out at the 10 PW laser system SULF at
SIOM/Shanghai.Comment: 7 pages, 7 figure
The ABC Effect in Double-Pionic Nuclear Fusion and a pn Resonance as its Possible Origin
The ABC effect -- a long-standing puzzle in double-pionic fusion -- has been
reexamined by the first exclusive and kinematically complete measurements of
solid statistics for the fusion reactions , He and He using the WASA detector, first at
CELSIUS and recently at COSY -- the latter with a statistics increased by
another two orders of magnitude. In all cases we observe a huge low-mass
enhancement in the -invariant mass accompanied by a pronounced
excitation. For the most basic fusion reaction, the reaction, we observe in addition a very pronounced resonance-like
energy dependence in the total cross section with a maximum 90 MeV below the
mass and a width of only 50 MeV, which is five times smaller
than expected from a conventional -channel excitation. This
reveals the ABC effect to be the consequence of a s-channel resonance with the
formfactor of this dibaryonic state being reflected in the low-mass enhancement
of the -invariant mass. From the fusion reactions to He and He
we learn that this resonance is robust enough to survive even in nuclei.Comment: conference proceedings PANIC 0
Phenomenological analysis of K+ meson production in proton-nucleus collisions
Total and differential cross sections from literature, on the production of
K+ mesons in pA interactions at projectile energies between T=0.8 and 2.9 GeV,
covering the transition across the free nucleon-nucleon threshold at 1.58 GeV,
have been investigated. From the target-mass dependence of the production cross
sections no evidence for the expected change of the dominant reaction mechanism
from two-step to direct kaon production was found. At T=1.0 GeV the A
dependences of the total cross sections and of the most recent data from
COSY-Juelich, differential cross sections measured under forward angles, are
strongly different. The invariant K+ production cross sections show an overall
exponential scaling behavior with the squared four-momentum transfer between
the beam proton and the produced K+ meson for t< -0.05 GeV^2 independent of the
beam energy and emission angle. The data from COSY-Juelich reveal a strongly
different t dependence in the region of t>0 GeV^2. Further data at forward
angles and different beam energies should be taken in order to explore this
region of kinematically extreme conditions.Comment: 9 Pages, 11 Figure
Shape Isomerism at N = 40: Discovery of a Proton Intruder in 67Co
The nuclear structure of 67Co has been investigated through 67Fe beta-decay.
The 67Fe isotopes were produced at the LISOL facility in proton-induced fission
of 238U and selected using resonant laser ionization combined with mass
separation. The application of a new correlation technique unambiguously
revealed a 496(33) ms isomeric state in 67Co at an unexpected low energy of 492
keV. A 67Co level scheme has been deduced. Proposed spin and parities suggest a
spherical (7/2-) 67Co ground state and a deformed first excited (1/2-) state at
492 keV, interpreted as a proton 1p-2h prolate intruder state.Comment: 4 pages, 5 figures, preprint submitted to Physical Review Letter
Eta and eta-prime meson production in the reaction pn -> dM near threshold
The two-step model with pi-, rho- and omega- exchanges taken into account is
applied to investigate the reactions pn -> d eta and pn -> d eta-prime. The
existing experimental data on the reaction pn -> d eta are analysed and
predictions for the cross section of the reaction pn -> d eta-prime are
presented. It is found that pi- as well as rho exchange yield significant
contributions in both reactions. The effect of the final state interaction is
also studied.Comment: 15 pages, 4 figure
The tactical mimicry of social enterprise strategies: acting ‘as if’ in the everyday life of third sector organizations
Using England as a paradigmatic case of the „enterprising up ‟ of the third sector through social enterprise policies and programs, this article sheds light on resistance as enacted through dramaturgical identification with government strategies. Drawing from a longitudinal qualitative research study, which is interpreted via Michel de Certeau‟s theory of the everyday, we present the case study of Teak, a charitable regeneration company, to illustrate how its Chief Executive Liam „acted as ‟ a social entrepreneur in order to gain access to important resources. We establish „tactical mimicry ‟ as a sensitizing concept to suggest that third sector practitioners ‟ identification with the normative premises of „social enterprise ‟ is part of a parasitical prosaics geared toward appropriating public money. While tactical mimicry conforms to strategies only in order to exploit them, its ultimate aim is to increase potentials of collective agency outside the direct influence of power. The contribution we make is threefold: first, we extend the recent debate on productive resistance by highlighting how „playing the game ‟ without changing existing relations of power can nevertheless produce largely favorable outcomes. Second, we suggest that recognition of the productive potential of tactical mimicry requires methodologies which pay attention to the spatial and temporal dynamics of resistance. And third, we argue that explaining „social enterprise‟ without consideration of the non-discursive, mainly financial resources made available to those who identify with it, necessarily risks overlooking a crucial element of the dramaturgical dynamic of discourse
Subthreshold antiproton production in proton-carbon reactions
Data from KEK on subthreshold antiproton as well as on pi(+-) and K(+-)
production in proton-nucleus reactions are described at projectile energies
between 3.5 and 12.0 GeV. We use a model which considers a hadron-nucleus
reaction as an incoherent sum over collisions of the projectile with a varying
number of target nucleons. It samples complete events and allows thus for the
simultaneous consideration of all particle species measured. The overall
reproduction of the data is quite satisfactory. It is shown that the
contributions from the interaction of the projectile with groups of several
target nucleons are decisive for the description of subthreshold production.
Since the collective features of subthreshold production become especially
significant far below the threshold, the results are extrapolated down to COSY
energies. It is concluded that an antiproton measurement at ANKE-COSY should be
feasible, if the high background of other particles can be efficiently
suppressed.Comment: 15 pages, 5 figures, gzipped tar file, submitted to J. Phys. G v2:
Modification of text due to demands of referee
- …