An inner ring and the micro lensing toward the Bulge

All current Bulge-Disk models for the inner Galaxy fall short of reproducing self-consistently the observed micro-lensing optical depth by a factor of two ($> 2\sigma$). We show that the least mass-consuming way to increase the optical depth is to add density roughly half-way the observer and the highest micro-lensing-source density. We present evidence for the existence of such a density structure in the Galaxy: an inner ring, a standard feature of barred galaxies. Judging from data on similar rings in external galaxies, an inner ring can contribute more than 50% of a pure Bulge-Disk model to the micro-lensing optical depth. We may thus eliminate the need for a small viewing angle of the Bar. The influence of an inner ring on the event-duration distribution, for realistic viewing angles, would be to increase the fraction of long-duration events toward Baade's window. The longest events are expected toward the negative-longitude tangent point at $\ell\sim$ -22\degr . A properly sampled event-duration distribution toward this tangent point would provide essential information about viewing angle and elongation of the over-all density distribution in the inner Galaxy.Comment: 9 pages, 7(15) figs, LaTeX, AJ (accepted

The RNA modification database, RNAMDB: 2011 update

Since its inception in 1994, The RNA Modification Database (RNAMDB, http://rna-mdb.cas.albany.edu/RNAmods/) has served as a focal point for information pertaining to naturally occurring RNA modifications. In its current state, the database employs an easy-to-use, searchable interface for obtaining detailed data on the 109 currently known RNA modifications. Each entry provides the chemical structure, common name and symbol, elemental composition and mass, CA registry numbers and index name, phylogenetic source, type of RNA species in which it is found, and references to the first reported structure determination and synthesis. Though newly transferred in its entirety to The RNA Institute, the RNAMDB continues to grow with two notable additions, agmatidine and 8-methyladenosine, appended in the last year. The RNA Modification Database is staying up-to-date with significant improvements being prepared for inclusion within the next year and the following year. The expanded future role of The RNA Modification Database will be to serve as a primary information portal for researchers across the entire spectrum of RNA-related research

Detection of a flow induced magnetic field eigenmode in the Riga dynamo facility

In an experiment at the Riga sodium dynamo facility, a slowly growing magnetic field eigenmode has been detected over a period of about 15 seconds. For a slightly decreased propeller rotation rate, additional measurements showed a slow decay of this mode. The measured results correspond satisfactory with numerical predictions for the growth rates and frequencies

Using virtual experiences of older age: exploring pedagogical and psychological experiences of students

Fostering intergenerational empathy is vital for creating an age-friendly society and an important aim for Sport and Exercise Science (SES) degree programmes given that graduates are increasingly entering the healthcare workforce supporting older adults (British Association of Sport and Exercise Sciences; BASES, 2018). Interventions to challenge negative stereotypes of ageing, generate empathy for older adults, and help University students gain experience of ‘being’ an older person have demonstrated mixed success (e.g., Prior & Sargent-Cox, 2014). Recent studies indicate the promise of virtual reality in this context but do not present conclusive evidence for this effect (e.g., Banakou, Kishore, & Slater, 2018). Thus this study explored SES students’ responses to virtual experiences of being an older person in a workshop. Participants completed the “Become Victor” module of the FrailtySIM© application, based on real life experience of an older person in their home, and, a University-developed immersive experience of being an older person in a social situation. Fifty-two students completed questionnaires about their experience of “Become Victor” and 15 students were interviewed (12 in 2 focus groups, 3 individually) about their experiences of both simulations. Data indicated that “Become Victor” offered students insight into being an older person that was “eye-opening” and realistic but frustrating and stressful. The social situation effectively simulated the isolation felt by some older people to an extent, but needed to be more interactive. Students felt that the simulations were important for contextualising previously delivered lecture material on older adults. Future workshop iterations will integrate lecture and virtual experiences using opportunities for student reflection on their experiences

BSL-1K: Scaling up co-articulated sign language recognition using mouthing cues

Recent progress in fine-grained gesture and action classification, and machine translation, point to the possibility of automated sign language recognition becoming a reality. A key stumbling block in making progress towards this goal is a lack of appropriate training data, stemming from the high complexity of sign annotation and a limited supply of qualified annotators. In this work, we introduce a new scalable approach to data collection for sign recognition in continuous videos. We make use of weakly-aligned subtitles for broadcast footage together with a keyword spotting method to automatically localise sign-instances for a vocabulary of 1,000 signs in 1,000 hours of video. We make the following contributions: (1) We show how to use mouthing cues from signers to obtain high-quality annotations from video data - the result is the BSL-1K dataset, a collection of British Sign Language (BSL) signs of unprecedented scale; (2) We show that we can use BSL-1K to train strong sign recognition models for co-articulated signs in BSL and that these models additionally form excellent pretraining for other sign languages and benchmarks - we exceed the state of the art on both the MSASL and WLASL benchmarks. Finally, (3) we propose new large-scale evaluation sets for the tasks of sign recognition and sign spotting and provide baselines which we hope will serve to stimulate research in this area.Comment: Appears in: European Conference on Computer Vision 2020 (ECCV 2020). 28 page

Dynamics of the Galactic Bulge using Planetary Nebulae

Evidence for a bar at the center of the Milky Way triggered a renewed enthusiasm for dynamical modelling of the Galactic bar-bulge. Our goal is to compare the kinematics of a sample of tracers, planetary nebulae, widely distributed over the bulge with the corresponding kinematics for a range of models of the inner Galaxy. Three of these models are N-body barred systems arising from the instabilities of a stellar disk (Sellwood, Fux and Kalnajs), and one is a Schwarzschild system constructed to represent the 3D distribution of the COBE/DIRBE near-IR light and then evolved as an N-body system for a few dynamical times (Zhao). For the comparison of our data with the models, we use a new technique developed by Saha (1998). The procedure finds the parameters of each model, i.e. the solar galactocentric distance R_o in model units, the orientation angle phi, the velocity scale (in km/s per model unit), and the solar tangential velocity which best fit the data.Comment: 48 pages (Latex), 30 figures (PS), accepted for pub. in A

THUMP from archaeal tRNA:m(2)(2)G10 methyltransferase, a genuine autonomously folding domain

The tRNA:m(2)(2)G10 methyltransferase of Pyrococus abyssi (PAB1283, a member of COG1041) catalyzes the N(2),N(2)-dimethylation of guanosine at position 10 in tRNA. Boundaries of its THUMP (THioUridine synthases, RNA Methyltransferases and Pseudo-uridine synthases)—containing N-terminal domain [1–152] and C-terminal catalytic domain [157–329] were assessed by trypsin limited proteolysis. An inter-domain flexible region of at least six residues was revealed. The N-terminal domain was then produced as a standalone protein (THUMPα) and further characterized. This autonomously folded unit exhibits very low affinity for tRNA. Using protein fold-recognition (FR) methods, we identified the similarity between THUMPα and a putative RNA-recognition module observed in the crystal structure of another THUMP-containing protein (ThiI thiolase of Bacillus anthracis). A comparative model of THUMPα structure was generated, which fulfills experimentally defined restraints, i.e. chemical modification of surface exposed residues assessed by mass spectrometry, and identification of an intramolecular disulfide bridge. A model of the whole PAB1283 enzyme docked onto its tRNA(Asp) substrate suggests that the THUMP module specifically takes support on the co-axially stacked helices of T-arm and acceptor stem of tRNA and, together with the catalytic domain, screw-clamp structured tRNA. We propose that this mode of interactions may be common to other THUMP-containing enzymes that specifically modify nucleotides in the 3D-core of tRNA

tRNA structural and functional changes induced by oxidative stress

Oxidatively damaged biomolecules impair cellular functions and contribute to the pathology of a variety of diseases. RNA is also attacked by reactive oxygen species, and oxidized RNA is increasingly recognized as an important contributor to neurodegenerative complications in humans. Recently, evidence has accumulated supporting the notion that tRNA is involved in cellular responses to various stress conditions. This review focuses on the intriguing consequences of oxidative modification of tRNA at the structural and functional level

The Human Mitochondrial tRNAMet: Structure/Function Relationship of a Unique Modification in the Decoding of Unconventional Codons

Human mitochondrial mRNAs utilize the universal AUG and the unconventional isoleucine AUA codons for methionine. In contrast to translation in the cytoplasm, human mitochondria use one tRNA, hmtRNAMetCAU, to read AUG and AUA codons at both the peptidyl- (P-), and aminoacyl-(A-) sites of the ribosome. The hmtRNAMetCAU has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position 34 (f5C34), and a cytidine substituting for the invariant uridine at position 33 of the canonical “U-turn” in tRNAs. The structure of the tRNA's anticodon stem and loop domain (hmtASLMetCAU), determined by NMR restrained molecular modeling, revealed how the f5C34 modification facilitates the decoding of AUA at the P- and A-sites. The f5C34 defined a reduced conformational space for the nucleoside, in what appears to have restricted the conformational dynamics of the anticodon bases of the modified hmtASLMetCAU. The hmtASLMetCAU exhibited a “C-turn” conformation that has some characteristics of the U-turn motif. Codon binding studies with both E. coli and bovine mitochondrial ribosomes revealed that the f5C34 facilitates AUA binding in the A-site and suggested that the modification favorably alters the ASL's binding kinetics. Mitochondrial translation by many organisms including humans sometimes initiates with the universal isoleucine codons AUU and AUC. The f5C34 enabled P-site codon binding to these normally isoleucine codons. Thus, the physicochemical properties of this one modification, f5C34, expand codon recognition from the traditional AUG to the non-traditional, synonymous codons AUU and AUC as well as AUA, in the reassignment of universal codons in the mitochondria