Photometric quality of Dome C for the winter 2008 from ASTEP South

ASTEP South is an Antarctic Search for Transiting Exo- Planets in the South pole field, from the Concordia station, Dome C, Antarctica. The instrument consists of a thermalized 10 cm refractor observing a fixed 3.88\degree x 3.88\degree field of view to perform photometry of several thousand stars at visible wavelengths (700-900 nm). The first winter campaign in 2008 led to the retrieval of nearly 1600 hours of data. We derive the fraction of photometric nights by measuring the number of detectable stars in the field. The method is sensitive to the presence of small cirrus clouds which are invisible to the naked eye. The fraction of night-time for which at least 50% of the stars are detected is 74% from June to September 2008. Most of the lost time (18.5% out of 26%) is due to periods of bad weather conditions lasting for a few days ("white outs"). Extended periods of clear weather exist. For example, between July 10 and August 10, 2008, the total fraction of time (day+night) for which photometric observations were possible was 60%. This confirms the very high quality of Dome C for nearly continuous photometric observations during the Antarctic winter

ASTEP South: An Antarctic Search for Transiting ExoPlanets around the celestial South pole

ASTEP South is the first phase of the ASTEP project (Antarctic Search for Transiting ExoPlanets). The instrument is a fixed 10 cm refractor with a 4kx4k CCD camera in a thermalized box, pointing continuously a 3.88 degree x 3.88 degree field of view centered on the celestial South pole. ASTEP South became fully functional in June 2008 and obtained 1592 hours of data during the 2008 Antarctic winter. The data are of good quality but the analysis has to account for changes in the point spread function due to rapid ground seeing variations and instrumental effects. The pointing direction is stable within 10 arcseconds on a daily timescale and drifts by only 34 arcseconds in 50 days. A truly continuous photometry of bright stars is possible in June (the noon sky background peaks at a magnitude R=15 arcsec-2 on June 22), but becomes challenging in July (the noon sky background magnitude is R=12.5 arcsec?2 on July 20). The weather conditions are estimated from the number of stars detected in the field. For the 2008 winter, the statistics are between 56.3 % and 68.4 % of excellent weather, 17.9 % to 30 % of veiled weather and 13.7 % of bad weather. Using these results in a probabilistic analysis of transit detection, we show that the detection efficiency of transiting exoplanets in one given field is improved at Dome C compared to a temperate site such as La Silla. For example we estimate that a year-long campaign of 10 cm refractor could reach an efficiency of 69 % at Dome C versus 45 % at La Silla for detecting 2-day period giant planets around target stars from magnitude 10 to 15. This shows the high potential of Dome C for photometry and future planet discoveries. [Short abstract

Preventing corona effects: multi-phosphonic acid poly(ethylene glycol) copolymers for stable stealth iron oxide nanoparticles

When disperse in biological fluids, engineered nanoparticles are selectively coated with proteins, resulting in the formation of a protein corona. It is suggested that the protein corona is critical in regulating the conditions of entry into the cytoplasm of living cells. Recent reports describe this phenomenon as ubiquitous and independent of the nature of the particle. For nanomedicine applications however, there is a need to design advanced and cost-effective coatings that are resistant to protein adsorption and that increase the biodistribution in vivo. In this study, phosphonic acid poly(ethylene glycol) copolymers were synthesized and used to coat iron oxide particles. The copolymer composition was optimized to provide simple and scalable protocols as well as long-term stability in culture media. It is shown that polymers with multiple phosphonic acid functionalities and PEG chains outperform other types of coating, including ligands, polyelectrolytes and carboxylic acid functionalized PEG. PEGylated particles exhibit moreover exceptional low cellular uptake, of the order of 100 femtograms of iron per cell. The present approach demonstrates that the surface chemistry of engineered particles is a key parameter in the interactions with cells. It also opens up new avenues for the efficient functionalization of inorganic surfaces.Comment: 21 page, 7 figures,Biomacromolecules 201

The secondary eclipses of WASP-19b as seen by the ASTEP 400 telescope from Antarctica

The ASTEP (Antarctica Search for Transiting ExoPlanets) program was originally aimed at probing the quality of the Dome C, Antarctica for the discovery and characterization of exoplanets by photometry. In the first year of operation of the 40 cm ASTEP 400 telescope (austral winter 2010), we targeted the known transiting planet WASP-19b in order to try to detect its secondary transits in the visible. This is made possible by the excellent sub-millimagnitude precision of the binned data. The WASP-19 system was observed during 24 nights in May 2010. The photometric variability level due to starspots is about 1.8% (peak-to-peak), in line with the SuperWASP data from 2007 (1.4%) and larger than in 2008 (0.07%). We find a rotation period of WASP-19 of 10.7 +/- 0.5 days, in agreement with the SuperWASP determination of 10.5 +/- 0.2 days. Theoretical models show that this can only be explained if tidal dissipation in the star is weak, i.e. the tidal dissipation factor Q'star > 3.10^7. Separately, we find evidence for a secondary eclipse of depth 390 +/- 190 ppm with a 2.0 sigma significance, a phase consistent with a circular orbit and a 3% false positive probability. Given the wavelength range of the observations (420 to 950 nm), the secondary transit depth translates into a day side brightness temperature of 2690(-220/+150) K, in line with measurements in the z' and K bands. The day side emission observed in the visible could be due either to thermal emission of an extremely hot day side with very little redistribution of heat to the night side, or to direct reflection of stellar light with a maximum geometrical albedo Ag=0.27 +/- 0.13. We also report a low-frequency oscillation well in phase at the planet orbital period, but with a lower-limit amplitude that could not be attributed to the planet phase alone, and possibly contaminated with residual lightcurve trends.Comment: Accepted for publication in Astronomy and Astrophysics, 13 pages, 13 figure

The GTC exoplanet transit spectroscopy survey IX. Detection of haze, Na, K, and Li in the super-Neptune WASP-127b

Exoplanets with relatively clear atmospheres are prime targets for detailed studies of chemical compositions and abundances in their atmospheres. Alkali metals have long been suggested to exhibit broad wings due to pressure broadening, but most of the alkali detections only show very narrow absorption cores, probably because of the presence of clouds. We report the strong detection of the pressure-broadened spectral profiles of Na, K, and Li absorption in the atmosphere of the super-Neptune WASP-127b, at 4.1$\sigma$, 5.0$\sigma$, and 3.4$\sigma$, respectively. We performed a spectral retrieval modeling on the high-quality optical transmission spectrum newly acquired with the 10.4 m Gran Telescopio Canarias (GTC), in combination with the re-analyzed optical transmission spectrum obtained with the 2.5 m Nordic Optical Telescope (NOT). By assuming a patchy cloudy model, we retrieved the abundances of Na, K, and Li, which are super-solar at 3.7$\sigma$ for K and 5.1$\sigma$ for Li (and only 1.8$\sigma$ for Na). We constrained the presence of haze coverage to be around 52%. We also found a hint of water absorption, but cannot constrain it with the global retrieval owing to larger uncertainties in the probed wavelengths. WASP-127b will be extremely valuable for atmospheric characterization in the era of James Webb Space Telescope

Complete high-intensity focused ultrasound in prostate cancer: outcome from the @-Registry

n/

Protein crystals in adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis

Intranuclear crystalline inclusions have been observed in the nucleus of epithelial cells infected with Adenovirus serotype 5 (Ad5) at late steps of the virus life cycle. Using immuno-electron microscopy and confocal microscopy of cells infected with various Ad5 recombinants modified in their penton base or fiber domains, we found that these inclusions represented crystals of penton capsomers, the heteromeric capsid protein formed of penton base and fiber subunits. The occurrence of protein crystals within the nucleus of infected cells required the integrity of the fiber knob and part of the shaft domain. In the knob domain, the region overlapping residues 489–492 in the FG loop was found to be essential for crystal formation. In the shaft, a large deletion of repeats 4 to 16 had no detrimental effect on crystal inclusions, whereas deletion of repeats 8 to 21 abolished crystal formation without altering the level of fiber protein expression. This suggested a crucial role of the five penultimate repeats in the crystallisation process. Chimeric pentons made of Ad5 penton base and fiber domains from different serotypes were analyzed with respect to crystal formation. No crystal was found when fiber consisted of shaft (S) from Ad5 and knob (K) from Ad3 (heterotypic S5-K3 fiber), but occurred with homotypic S3K3 fiber. However, less regular crystals were observed with homotypic S35-K35 fiber. TB5, a monoclonal antibody directed against the Ad5 fiber knob was found by immunofluorescence microscopy to react with high efficiency with the intranuclear protein crystals in situ. Data obtained with Ad fiber mutants indicated that the absence of crystalline inclusions correlated with a lower infectivity and/or lower yields of virus progeny, suggesting that the protein crystals might be involved in virion assembly. Thus, we propose that TB5 staining of Ad-infected 293 cells can be used as a prognostic assay for the viability and productivity of fiber-modified Ad5 vectors