40 research outputs found
The HIV positive selection mutation database
The HIV positive selection mutation database is a large-scale database available at that provides detailed selection pressure maps of HIV protease and reverse transcriptase, both of which are molecular targets of antiretroviral therapy. This database makes available for the first time a very large HIV sequence dataset (sequences from ∼50 000 clinical AIDS samples, generously contributed by Specialty Laboratories, Inc.), which makes possible high-resolution selection pressure mapping. It provides information about not only the selection pressure on individual sites but also how selection pressure at one site is affected by mutations on other sites. It also includes datasets from other public databases, namely the Stanford HIV database [S. Y. Rhee, M. J. Gonzales, R. Kantor, B. J. Betts, J. Ravela and R. W. Shafer (2003) Nucleic Acids Res., 31, 298–303]. Comparison between these datasets in the database enables cross-validation with independent datasets and also specific evaluation of the effect of drug treatment
Real-Time Data Driven Wildland Fire Modeling
We are developing a wildland fire model based on semi-empirical relations
that estimate the rate of spread of a surface fire and post-frontal heat
release, coupled with WRF, the Weather Research and Forecasting atmospheric
model. A level set method identifies the fire front. Data are assimilated using
both amplitude and position corrections using a morphing ensemble Kalman
filter. We will use thermal images of a fire for observations that will be
compared to synthetic image based on the model state.Comment: 8 pages, 4 figures. ICCS 0
Morphing Ensemble Kalman Filters
A new type of ensemble filter is proposed, which combines an ensemble Kalman
filter (EnKF) with the ideas of morphing and registration from image
processing. This results in filters suitable for nonlinear problems whose
solutions exhibit moving coherent features, such as thin interfaces in wildfire
modeling. The ensemble members are represented as the composition of one common
state with a spatial transformation, called registration mapping, plus a
residual. A fully automatic registration method is used that requires only
gridded data, so the features in the model state do not need to be identified
by the user. The morphing EnKF operates on a transformed state consisting of
the registration mapping and the residual. Essentially, the morphing EnKF uses
intermediate states obtained by morphing instead of linear combinations of the
states.Comment: 17 pages, 7 figures. Added DDDAS references to the introductio
A realtime observatory for laboratory simulation of planetary flows
Motivated by the large-scale circulation of the atmosphere and ocean, we develop a system that uses
observations from a laboratory analog to constrain, in real time, a numerical simulation of the laboratory
flow. This system provides a tool to rapidly prototype new methods for state and parameter
estimation, and facilitates the study of prediction, predictability, and transport of geophysical fluids
where observations or numerical simulations would not independently suffice.
A computer vision system is used to extract measurements of the physical simulation. Observations
are used to constrain the model-state of the MIT General Circulation Model in a probabilistic, ensemble based assimilation approach. Using a combination of parallelism, domain decomposition and an efficient
scheme to select ensembles of model-states, we show that estimates that effectively track the fluid state
can be produced. To the best of our knowledge this is the first such observatory for laboratory
analogs of planetary circulation that functions in real time.National Science Foundation (U.S.) (CNS-0540259)National Science Foundation (U.S.) (grant CNS-0540248
Comparison of HIV-1 Genotypic Resistance Test Interpretation Systems in Predicting Virological Outcomes Over Time
Background: Several decision support systems have been developed to interpret HIV-1 drug resistance genotyping results. This study compares the ability of the most commonly used systems (ANRS, Rega, and Stanford's HIVdb) to predict virological outcome at 12, 24, and 48 weeks. Methodology/Principal Findings: Included were 3763 treatment-change episodes (TCEs) for which a HIV-1 genotype was available at the time of changing treatment with at least one follow-up viral load measurement. Genotypic susceptibility scores for the active regimens were calculated using scores defined by each interpretation system. Using logistic regression, we determined the association between the genotypic susceptibility score and proportion of TCEs having an undetectable viral load (<50 copies/ml) at 12 (8-16) weeks (2152 TCEs), 24 (16-32) weeks (2570 TCEs), and 48 (44-52) weeks (1083 TCEs). The Area under the ROC curve was calculated using a 10-fold cross-validation to compare the different interpretation systems regarding the sensitivity and specificity for predicting undetectable viral load. The mean genotypic susceptibility score of the systems was slightly smaller for HIVdb, with 1.92±1.17, compared to Rega and ANRS, with 2.22±1.09 and 2.23±1.05, respectively. However, similar odds ratio's were found for the association between each-unit increase in genotypic susceptibility score and undetectable viral load at week 12; 1.6 [95% confidence interval 1.5-1.7] for HIVdb, 1.7 [1.5-1.8] for ANRS, and 1.7 [1.9-1.6] for Rega. Odds ratio's increased over time, but remained comparable (odds ratio's ranging between 1.9-2.1 at 24 weeks and 1.9-2.
Tracking Object Motion Across Aspect Changes for Augmented Reality
Amodel registration system capable of tracking an object through distinct aspects in real-time is presented. The system integrates tracking, pose determination, and aspect graph indexing. The tracking combines steerable #lters with normalizedcrosscorrelation, compensates for rotation in 2D and is adaptive. Robust statistical methods are used in the pose estimation to detect and remove mismatches. The aspect graph is used to determine when features will disappear or become di#cult to track and to predict when and where new features will become trackable. The overall system is stable and is amenable to real-time performance.