The Phoenix Deep Survey: The 1.4 GHz microJansky catalogue

The initial Phoenix Deep Survey (PDS) observations with the Australia Telescope Compact Array have been supplemented by additional 1.4 GHz observations over the past few years. Here we present details of the construction of a new mosaic image covering an area of 4.56 square degrees, an investigation of the reliability of the source measurements, and the 1.4 GHz source counts for the compiled radio catalogue. The mosaic achieves a 1-sigma rms noise of 12 microJy at its most sensitive, and a homogeneous radio-selected catalogue of over 2000 sources reaching flux densities as faint as 60 microJy has been compiled. The source parameter measurements are found to be consistent with the expected uncertainties from the image noise levels and the Gaussian source fitting procedure. A radio-selected sample avoids the complications of obscuration associated with optically-selected samples, and by utilising complementary PDS observations including multicolour optical, near-infrared and spectroscopic data, this radio catalogue will be used in a detailed investigation of the evolution in star-formation spanning the redshift range 0 < z < 1. The homogeneity of the catalogue ensures a consistent picture of galaxy evolution can be developed over the full cosmologically significant redshift range of interest. The 1.4 GHz mosaic image and the source catalogue are available on the web at http://www.atnf.csiro.au/~ahopkins/phoenix/ or from the authors by request.Comment: 16 pages, 11 figures, 4 tables. Accepted for publication by A

Smart home power management system for electric vehicle battery charger and electrical appliance control

This paper presents a power management system (PMS) designed for smart homes aiming to deal with the new challenges imposed by the proliferation of plug-in electric vehicles (EVs) and their coexistence with other residential electrical appliances. The PMS is based on a hybrid wireless network architecture composed by a local hub/gateway and several Bluetooth Low Energy (BLE) and Wi-Fi sensor/actuator devices. These wireless devices are used to transfer information inside the smart home using the MQTT (Message Queuing Telemetry Transport) protocol. Based on the proposed solution, the current consumption of the EV battery charger and other residential electrical appliances are dynamically monitored and controlled by using a configurable algorithm, ensuring that the total current consumption does not cause the tripping of the home circuit breaker. An Android client application allows the user to monitor and configure the system operation in real-time, a developed Wi Fi smart plug permits to measure the RMS values of current of the connected electrical appliance and change its state of operation remotely, and an EV battery charger may be controlled in terms of operating power according to set-points received from the Android client application. Experimental tests are used to evaluate the quality of service provided by the developed smart home platform in terms of communication delay and reliability. An experimental validation for different conditions of operation of the proposed smart home PMS concerning the power operation of the EV battery charger with the proposed control algorithm is also presented.info:eu-repo/semantics/acceptedVersio

Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo

Neutrophil recruitment from blood to extravascular sites of sterile or infectious tissue damage is a hallmark of early innate immune responses, and the molecular events leading to cell exit from the bloodstream have been well defined1,2. Once outside the vessel, individual neutrophils often show extremely coordinated chemotaxis and cluster formation reminiscent of the swarming behaviour of insects3,4,5,6,7,8,9,10,11. The molecular players that direct this response at the single-cell and population levels within the complexity of an inflamed tissue are unknown. Using two-photon intravital microscopy in mouse models of sterile injury and infection, we show a critical role for intercellular signal relay among neutrophils mediated by the lipid leukotriene B4, which acutely amplifies local cell death signals to enhance the radius of highly directed interstitial neutrophil recruitment. Integrin receptors are dispensable for long-distance migration12, but have a previously unappreciated role in maintaining dense cellular clusters when congregating neutrophils rearrange the collagenous fibre network of the dermis to form a collagen-free zone at the wound centre. In this newly formed environment, integrins, in concert with neutrophil-derived leukotriene B4 and other chemoattractants, promote local neutrophil interaction while forming a tight wound seal. This wound seal has borders that cease to grow in kinetic concert with late recruitment of monocytes and macrophages at the edge of the displaced collagen fibres. Together, these data provide an initial molecular map of the factors that contribute to neutrophil swarming in the extravascular space of a damaged tissue. They reveal how local events are propagated over large-range distances, and how auto-signalling produces coordinated, self-organized neutrophil-swarming behaviour that isolates the wound or infectious site from surrounding viable tissue

Microjansky sources at 1.4 GHz

We present a deep 1.4 GHz survey made with the Australia Telescope Compact Array (ATCA), having a background RMS of 9 microJy near the image phase centre, up to 25 microJy at the edge of a 50' field of view. Over 770 radio sources brighter than 45 microJy have been catalogued in the field. The differential source counts in the deep field provide tentative support for the growing evidence that the microjansky radio population exhibits significantly higher clustering than found at higher flux density cutoffs. The optical identification rate on CCD images is approximately 50% to R=22.5, and the optical counterparts of the faintest radio sources appear to be mainly single galaxies close to this optical magnitude limit.Comment: 6 pages, 4 figures, accepted by ApJ Letters 4 May 199

Detection and isolation of exotic Newcastle disease virus from field-collected flies.

Flies were collected by sweep net from the vicinity of two small groups of "backyard" poultry (10-20 chickens per group) that had been identified as infected with exotic Newcastle disease virus (family Paramyxoviridae, genus avulavirus, ENDV) in Los Angeles County, CA, during the 2002-2003 END outbreak. Collected flies were subdivided into pools and homogenized in brain-heart infusion broth with antibiotics. The separated supernatant was tested for the presence of ENDV by inoculation into embryonated chicken eggs. Exotic Newcastle disease virus was isolated from pools of Phaenicia cuprina (Wiedemann), Fannia canicularis (L.), and Musca domestica L., and it was identified by hemagglutination inhibition with Newcastle disease virus antiserum. Viral concentration in positive pools was low (&lt;1 egg infectious dose50 per fly). Isolated virus demonstrated identical monoclonal antibody binding profiles as well as 99% sequence homology in the 635-bp fusion gene sequence compared with ENDV recovered from infected commercial egg layer poultry during the 2002 outbreak

Hemoglobin genotype has minimal influence on the physiological response of juvenile atlantic cod (Gadus morhua) to environmental challenges

Hemoglobin (Hb) polymorphism in cod is associated with temperature‐related differences in biogeographical distribution, and several authors have suggested that functional characteristics of the various hemoglobin isoforms (HbIs) directly influence phenotypic traits such as growth rate. However, no study has directly examined whether Hb genotype translates into physiological differences at the whole animal level. Thus, we generated a family of juvenile Atlantic cod consisting of all three main Hb genotypes (HbI‐1/1, HbI‐2/2, and HbI‐1/2) by crossing a single pair of heterozygous parents, and we compared their metabolic and cortisol responses to an acute thermal challenge (10&deg;C to their critical thermal maximum [CTM] or 22&deg;C, respectively) and tolerance of graded hypoxia. There were no differences in routine metabolism (at 10&deg;C), maximum metabolic rate, metabolic scope, CTM (overall mean 22.9&deg; &plusmn; 0.2&deg;C), or resting and poststress plasma cortisol levels among Hb genotypes. Further, although the HbI‐1/1 fish grew more (by 15%&ndash;30% during the first 9 mo) when reared at 10&deg; &plusmn; 1&deg;C and had a slightly enhanced hypoxia tolerance at 10&deg;C (e.g., the critical O2 levels for HbI‐1/1, HbI‐2/2, and HbI‐1/2 cod were 35.56% &plusmn; 1.24%, and 40.20% &plusmn; 1.99% air saturation, respectively), these results are contradictory to expectations based on HbI functional properties. Thus, our findings (1) do not support previous assumptions that growth rate differences among cod Hb genotypes result from a more efficient use of the oxygen supply&mdash;that is, reduced standard metabolic rates and/or increased metabolic capacity&mdash;and (2) suggest that in juvenile cod, there is no selective advantage to having a particular Hb genotype with regards to the capacity to withstand ecologically relevant environmental challenges.<br /

Gem-induced cytoskeleton remodeling increases cellular migration of HTLV-1-infected cells, formation of infected-to-target T-cell conjugates and viral transmission

Efficient HTLV-1 viral transmission occurs through cell-to-cell contacts. The Tax viral transcriptional activator protein facilitates this process. Using a comparative transcriptomic analysis, we recently identified a series of genes up-regulated in HTLV-1 Tax expressing T-lymphocytes. We focused our attention towards genes that are important for cytoskeleton dynamic and thus may possibly modulate cell-to-cell contacts. We first demonstrate that Gem, a member of the small GTP-binding proteins within the Ras superfamily, is expressed both at the RNA and protein levels in Tax-expressing cells and in HTLV-1-infected cell lines. Using a series of ChIP assays, we show that Tax recruits CREB and CREB Binding Protein (CBP) onto a c-AMP Responsive Element (CRE) present in the gem promoter. This CRE sequence is required to drive Tax-activated gem transcription. Since Gem is involved in cytoskeleton remodeling, we investigated its role in infected cells motility. We show that Gem co-localizes with F-actin and is involved both in T-cell spontaneous cell migration as well as chemotaxis in the presence of SDF-1/CXCL12. Importantly, gem knock-down in HTLV-1-infected cells decreases cell migration and conjugate formation. Finally, we demonstrate that Gem plays an important role in cell-to-cell viral transmission

Subcutaneous Sarcoidosis. A Rare Case of Specific Cutaneous Involvement

A sarcoidose é uma doença granulomatosa multissistémica de etiologia desconhecida. O envolvimento cutâneo pode ocorrer, sendo classificado de específico ou inespecífico, dependendo da presença ou ausência de granulomas no exame histopatológico da pele. A forma subcutânea é uma forma particular e mais rara de apresentação e constitui o único subtipo que se crê estar associado a doença sistémica

Control of sleep by a network of cell cycle genes

Sleep is essential for health and cognition, but the molecular and neural mechanisms of sleep regulation are not well understood. We recently reported the identification of TARANIS (TARA) as a sleep-promoting factor that acts in a previously unknown arousal center in Drosophila. tara mutants exhibit a dose-dependent reduction in sleep amount of up to ∼60%. TARA and its mammalian homologs, the Trip-Br (Transcriptional Regulators Interacting with PHD zinc fingers and/or Bromodomains) family of proteins, are primarily known as transcriptional coregulators involved in cell cycle progression, and contain a conserved Cyclin-A (CycA) binding homology domain. We found that tara and CycA synergistically promote sleep, and CycA levels are reduced in tara mutants. Additional data demonstrated that Cyclin-dependent kinase 1 (Cdk1) antagonizes tara and CycA to promote wakefulness. Moreover, we identified a subset of CycA expressing neurons in the pars lateralis, a brain region proposed to be analogous to the mammalian hypothalamus, as an arousal center. In this Extra View article, we report further characterization of tara mutants and provide an extended discussion of our findings and future directions within the framework of a working model, in which a network of cell cycle genes, tara, CycA, and Cdk1, interact in an arousal center to regulate sleep.This work was supported by a grant from the National Institutes of Health (R01NS086887 to K.K.) and predoctoral fellowships from the Portuguese Foundation for Science and Technology (SFRH/BD/51726/2011 to D.J.S.A and SFRH/BD/52321/2013 to D.R.M)