Temperature-activity relationships in Meligethes aeneus: implications for pest management

BACKGROUNDPollen beetle (Meligethes aeneus F.) management in oilseed rape (Brassica napus L.) has become an urgent issue in the light of insecticide resistance. Risk prediction advice has relied upon flight temperature thresholds, while risk assessment uses simple economic thresholds. However, there is variation in the reported temperature of migration, and economic thresholds vary widely across Europe, probably owing to climatic factors interacting with beetle activity and plant compensation for damage. The effect of temperature on flight, feeding and oviposition activity of M. aeneus was examined in controlled conditions. RESULTSEscape from a release vial was taken as evidence of flight and was supported by video observations. The propensity to fly followed a sigmoid temperature-response curve between 6 and 23 degrees C; the 10, 25 and 50% flight temperature thresholds were 12.0-12.5 degrees C, 13.6-14.2 degrees C and 15.5-16.2 degrees C, respectively. Thresholds were slightly higher in the second of two flight bioassays, suggesting an effect of beetle age. Strong positive relationships were found between temperature (6-20 degrees C) and the rates of feeding and oviposition on flower buds of oilseed rape. CONCLUSIONThese temperature relationships could be used to improve M. aeneus migration risk assessment, refine weather-based decision support systems and modulate damage thresholds according to rates of bud damage. (c) 2014 Society of Chemical Industr

The electronic frailty index as an indicator of community healthcare service utilisation in the older population

Background: older people with frailty are particularly high users of healthcare services, however a lack of standardised recording of frailty in different healthcare electronic datasets has limited investigations into healthcare service usage and demand of the older frail population. Objectives: to investigate the community service demand of frail patients using the electronic frailty index (eFI) as a measure of frailty. Study design and setting a retrospective cohort study using anonymised linked healthcare patient data from primary care, community services and acute hospitals in Norfolk. Participants: patients aged 65 and over who had an eFI assessment score established in their primary care electronic patient record in Norwich based General Practices. Results: we include data from 22,859 patients with an eFI score. Frailty severity increased with age and was associated with increased acute hospital admission within a 6-month window. Patients with a frail eFI score were also more likely to have a community service referral within a 6-month window of frailty assessment, with a RR of 1.84 (1.76–1.93) for mild frailty, 1.96 (1.83–2.09) for moderate frailty and 2.95 (2.76–3.14) for severe frailty scores. We also found that frail patients had more community referrals per patient then those classified as fit and required more care plans per community referral. Conclusions: eFI score was an indicator of community service use, with increasing severity of frailty being associated with higher community healthcare requirements. The eFI may help planning of community services for the frail population

Supporting local diversity of habitats and species on farmland: a comparison of three wildlife-friendly schemes

Restoration and maintenance of habitat diversity have been suggested as conservation priorities in farmed landscapes, but how this should be achieved and at what scale are unclear. This study makes a novel comparison of the effectiveness of three wildlife-friendly farming schemes for supporting local habitat diversity and species richness on 12 farms in England. The schemes were: (i) Conservation Grade (Conservation Grade: a prescriptive, non-organic, biodiversity-focused scheme), (ii) organic agriculture and (iii) a baseline of Entry Level Stewardship (Entry Level Stewardship: a flexible widespread government scheme). Conservation Grade farms supported a quarter higher habitat diversity at the 100-m radius scale compared to Entry Level Stewardship farms. Conservation Grade and organic farms both supported a fifth higher habitat diversity at the 250-m radius scale compared to Entry Level Stewardship farms. Habitat diversity at the 100-m and 250-m scales significantly predicted species richness of butterflies and plants. Habitat diversity at the 100-m scale also significantly predicted species richness of birds in winter and solitary bees. There were no significant relationships between habitat diversity and species richness for bumblebees or birds in summer. Butterfly species richness was significantly higher on organic farms (50% higher) and marginally higher on Conservation Grade farms (20% higher), compared with farms in Entry Level Stewardship. Organic farms supported significantly more plant species than Entry Level Stewardship farms (70% higher) but Conservation Grade farms did not (10% higher). There were no significant differences between the three schemes for species richness of bumblebees, solitary bees or birds. Policy implications. The wildlife-friendly farming schemes which included compulsory changes in management, Conservation Grade and organic, were more effective at increasing local habitat diversity and species richness compared with the less prescriptive Entry Level Stewardship scheme. We recommend that wildlife-friendly farming schemes should aim to enhance and maintain high local habitat diversity, through mechanisms such as option packages, where farmers are required to deliver a combination of several habitats

Understanding face detection with visual arrays and real-world scenes

Face detection has been studied by presenting faces in blank displays, object arrays, and real-world scenes. This study investigated whether these display contexts differ in what they can reveal about detection, by comparing frontal-view faces with those shown in profile (Experiment 1), rotated by 90º (Experiment 2), or turned upside-down (Experiment 3). In blank displays, performance for all face conditions was equivalent, whereas upright frontal faces showed a consistent detection advantage in arrays and scenes. Experiment 4 examined which facial characteristics drive this detection advantage by rotating either the internal or external facial features by 90º while the other features remained upright. Faces with rotated internal features were detected as efficiently as their intact frontal counterparts, whereas detection was impaired when external features were rotated. Finally, Experiment 5 applied Voronoi transformations to scenes to confirm that complexity of stimulus displays modulates the detection advantage for upright faces. These experiments demonstrate that context influences what can be learned about the face detection process. In complex visual arrays and natural scenes, detection proceeds more effectively when external facial features are preserved in an upright orientation. These findings are consistent with a cognitive detection template that focuses on general face-shape information

High-throughput monitoring of wild bee diversity and abundance via mitogenomics

1. Bee populations and other pollinators face multiple, synergistically acting threats, which have led to population declines, loss of local species richness and pollination services, and extinctions. However, our understanding of the degree, distribution and causes of declines is patchy, in part due to inadequate monitoring systems, with the challenge of taxonomic identification posing a major logistical barrier. Pollinator conservation would benefit from a high-throughput identification pipeline. 2. We show that the metagenomic mining and resequencing of mitochondrial genomes (mitogenomics) can be applied successfully to bulk samples of wild bees. We assembled the mitogenomes of 48 UK bee species and then shotgun-sequenced total DNA extracted from 204 whole bees that had been collected in 10 pan-trap samples from farms in England and been identified morphologically to 33 species. Each sample data set was mapped against the 48 reference mitogenomes. 3. The morphological and mitogenomic data sets were highly congruent. Out of 63 total species detections in the morphological data set, the mitogenomic data set made 59 correct detections (93�7% detection rate) and detected six more species (putative false positives). Direct inspection and an analysis with species-specific primers suggested that these putative false positives were most likely due to incorrect morphological IDs. Read frequency significantly predicted species biomass frequency (R2 = 24�9%). Species lists, biomass frequencies, extrapolated species richness and community structure were recovered with less error than in a metabarcoding pipeline. 4. Mitogenomics automates the onerous task of taxonomic identification, even for cryptic species, allowing the tracking of changes in species richness and istributions. A mitogenomic pipeline should thus be able to contain costs, maintain consistently high-quality data over long time series, incorporate retrospective taxonomic revisions and provide an auditable evidence trail. Mitogenomic data sets also provide estimates of species counts within samples and thus have potential for tracking population trajectories

Asymptotics of Eigenvalues and Eigenfunctions for the Laplace Operator in a Domain with Oscillating Boundary. Multiple Eigenvalue Case

We study the asymptotic behavior of the solutions of a spectral problem for the Laplacian in a domain with rapidly oscillating boundary. We consider the case where the eigenvalue of the limit problem is multiple. We construct the leading terms of the asymptotic expansions for the eigenelements and verify the asymptotics

Shiga Toxin and Lipopolysaccharide Induce Platelet-Leukocyte Aggregates and Tissue Factor Release, a Thrombotic Mechanism in Hemolytic Uremic Syndrome

BACKGROUND: Aggregates formed between leukocytes and platelets in the circulation lead to release of tissue factor (TF)-bearing microparticles contributing to a prothrombotic state. As enterohemorrhagic Escherichia coli (EHEC) may cause hemolytic uremic syndrome (HUS), in which microthrombi cause tissue damage, this study investigated whether the interaction between blood cells and EHEC virulence factors Shiga toxin (Stx) and lipopolysaccharide (LPS) led to release of TF. METHODOLOGY/PRINCIPAL FINDINGS: The interaction between Stx or LPS and blood cells induced platelet-leukocyte aggregate formation and tissue factor (TF) release, as detected by flow cytometry in whole blood. O157LPS was more potent than other LPS serotypes. Aggregates formed mainly between monocytes and platelets and less so between neutrophils and platelets. Stimulated blood cells in complex expressed activation markers, and microparticles were released. Microparticles originated mainly from platelets and monocytes and expressed TF. TF-expressing microparticles, and functional TF in plasma, increased when blood cells were simultaneously exposed to the EHEC virulence factors and high shear stress. Stx and LPS in combination had a more pronounced effect on platelet-monocyte aggregate formation, and TF expression on these aggregates, than each virulence factor alone. Whole blood and plasma from HUS patients (n = 4) were analyzed. All patients had an increase in leukocyte-platelet aggregates, mainly between monocytes and platelets, on which TF was expressed during the acute phase of disease. Patients also exhibited an increase in microparticles, mainly originating from platelets and monocytes, bearing surface-bound TF, and functional TF was detected in their plasma. Blood cell aggregates, microparticles, and TF decreased upon recovery. CONCLUSIONS/SIGNIFICANCE: By triggering TF release in the circulation, Stx and LPS can induce a prothrombotic state contributing to the pathogenesis of HUS

The Subtle Politics of Self-esteem Programs for Girls

Our purpose in this chapter is critique, not as an end in itself, but as a possible stimulus towards new directions in our thinking on the issue of girls and schooling. We offer this study in the belief that as feminist educators we must be constantly and restlessly critical, not only of the implications of the sex/gender system for members of our sex, but also of our own discourses. For if feminism cannot criticize itself, it cannot ‘serve as the bearer of emancipatory possibilities that can never be fixed and defined once and for all’ (Elshtain, 1982, p. 136). Our focus is upon the selfesteem discourse as it manifests itself in discussions of girls’ education. We believe it is important that a form of ‘ideology critique’ is conducted on this literature for the following reasons. Discourses often direct and affect our behaviour in subtle ways which we are not particularly conscious of and have wider social implications which, on quick inspection, are not apparent. Dredging such ‘subtexts’ to the surface provides us with a better sense of what we are meaning to others and to ourselves. It also allows us to ask whether this is what we intended to mean and, if not, what of our language, imagery, style, logic, etc. we need to change so that the effects of our politics may more closely resemble our intentions