Rapid bioconcentration of steroids in the plasma of three-spined stickleback Gasterosteus aculeatus exposed to waterborne testosterone and 17β-oestradiol

The relationship over time between the concentrations of two steroids, singly and in combination, in a static exposure system and in the blood of three-spined stickleback Gasterosteus aculeatus, held within the exposure system was investigated. Groups of three-spined stickleback were exposed (nominally) to either 1000 ng l1 17β-oestradiol (E2), testosterone (T) or E2 and T in combination at the same concentrations for 6 days. Both water and fish were sampled at intervals and steroid concentrations in both compartments were determined. The plasma steroid time profile revealed a rapid bioconcentration within the first 6 h of exposure. The plasma steroid levels attained at this time point (20–90 ng ml1) were up to 50-fold (E2) and 200-fold (T) greater than the actual levels of steroid measured in the exposure water, while levels in the blood of control fish did not exceed 4 ng ml1. The substantial elevation of plasma steroid levels relative to the concentrations of steroid to which the fish were exposed in the ambient water gives scope for delivery of the steroids to target endocrine tissues at levels far in excess of what might be predicted on the basis of passive branchial uptake alone. These results are discussed in relation to endocrine disruption, and in particular the occurrence of effects in fish exposed to levels of endocrine active substances that are seemingly physiologically irrelevant

Warming has stronger direct than indirect effects on benthic microalgae in a seaweed system in spring

Using outdoor mesocosms we investigated the relative importance of the direct and indirect (here: altered grazing) effects of seawater warming on benthic microalgae in a Baltic Sea Fucus vesiculosus (Phaeophyceae) system during the spring season. Seawater warming had a positive main effect on microalgal total biomass accrual and growth rate and on total mesograzer abundance and biomass. Moreover, under the existing resource-replete conditions in spring the direct positive effect of warming on microalgae was stronger than its indirect negative effect through enhanced grazing. The outcome of this study contrasts previous observations from the summer and winter season, where indirect effects of warming mediated by altered grazing were identified as an important driver of primary biomass in the Fucus system. In this context, the results from the spring season add mechanistic information to the overall understanding of the seasonal variability of climate change effects. They suggest that the relative importance of the underlying direct and indirect effective pathways of warming and the overall effect on the balance between production and consumption are influenced by the trophic state of the system, which in temperate regions is related to season

Convective Fingering of an Autocatalytic Reaction Front

We report experimental observations of the convection-driven fingering instability of an iodate-arsenous acid chemical reaction front. The front propagated upward in a vertical slab; the thickness of the slab was varied to control the degree of instability. We observed the onset and subsequent nonlinear evolution of the fingers, which were made visible by a {\it p}H indicator. We measured the spacing of the fingers during their initial stages and compared this to the wavelength of the fastest growing linear mode predicted by the stability analysis of Huang {\it et. al.} [{\it Phys. Rev. E}, {\bf 48}, 4378 (1993), and unpublished]. We find agreement with the thickness dependence predicted by the theory.Comment: 11 pages, RevTex with 3 eps figures. To be published in Phys Rev E, [email protected], [email protected], [email protected]

Communication in Hong Kong accident and emergency departments: The clinicians’ perspectives

© The Author(s) 2015. In this article, we report findings from the first qualitatively driven study of patient–clinician communication in Hong Kong Accident and Emergency Departments (AEDs). In light of the Hong Kong Hospital Authority’s policy emphasis on patientcentered care and communication in the public hospitals it oversees, we analyze clinicians’ perceptions of the role and relevance of patient-centered communication strategies in emergency care. Although aware of the importance of effective communication in emergency care, participants discussed how this was frequently jeopardized by chronic understaffing, patient loads, and time pressures. This was raised in relation to the absence of spoken interdisciplinary handovers, the tendency to downgrade interpersonal communication with patients, and the decline in staff attendance at communication training courses. Participants’ frequent descriptions of patient-centered communication as dispensable from, and timeburdensome in, AEDs highlight a discrepancy between the stated Hong Kong Hospital Authority policy of patient-centered care and the reality of contemporary Hong Kong emergency practice

Benthic phosphorus cycling within the Eurasian marginal sea ice zone

The Arctic Ocean region is currently undergoing dramatic changes, which will likely alter the nutrient cycles that underpin Arctic marine ecosystems. Phosphate is a key limiting nutrient for marine life but gaps in our understanding of the Arctic phosphorus (P) cycle persist. In this study, we investigate the benthic burial and recycling of phosphorus using sediments and pore waters from the Eurasian Arctic margin, including the Barents Sea slope and the Yermak Plateau. Our results highlight that P is generally lost from sediments with depth during organic matter respiration. On the Yermak Plateau, remobilization of P results in a diffusive flux of P to the seafloor of between 96 and 261 µmol m−2 yr−1. On the Barents Sea slope, diffusive fluxes of P are much larger (1736–2449 µmol m−2 yr−1), but these fluxes are into near-surface sediments rather than to the bottom waters. The difference in cycling on the Barents Sea slope is controlled by higher fluxes of fresh organic matter and active iron cycling. As changes in primary productivity, ocean circulation and glacial melt continue, benthic P cycling is likely to be altered with implications for P imported into the Arctic Ocean Basin

Expedition 302 summary

The first scientific drilling expedition to the central Arctic Ocean was completed in September 2004. Integrated Ocean Drilling Program Expedition 302, Arctic Coring Expedition (ACEX), recovered sediment cores to 428 meters below seafloor (mbsf) in water depths of ~1300 m, 250 km from the North Pole.Expedition 302's destination was the Lomonosov Ridge, hypothesized to be a sliver of continental crust that broke away from the Eurasian plate at ~56 Ma. As the ridge moved northward and subsided, marine sedimentation occurred and continues to the present, resulting in what was anticipated from seismic data to be a continuous paleoceanographic record. The elevation of the ridge above the surrounding abyssal plains (~3 km) ensured that sediments atop the ridge were free of turbidites. The primary scientific objective of Expedition 302 was to continuously recover this sediment record and to sample the underlying sedimentary bedrock by drilling and coring from a stationary drillship.The biggest challenge during Expedition 302 was maintaining the drillship's location while drilling and coring in 2–4 m thick sea ice that moved at speeds approaching 0.5 kt. Sea-ice cover over the Lomonosov Ridge moves with one of the two major Arctic sea-ice circulation systems, the Transpolar Drift, and responds locally to wind, tides, and currents. Until now, the high Arctic Ocean Basin, known as "mare incognitum" within the scientific community, had never before been deeply cored because of these challenging sea-ice conditions.Initial results reveal that biogenic carbonate is present only in the Holocene–Pleistocene interval. The upper 198 mbsf represents a relatively high sedimentation rate record of the past 18 m.y. and is composed of sediment with ice-rafted debris and dropstones, suggesting that ice-covered conditions extended at least this far back in time. Details of the ice type (e.g., iceberg versus sea ice), timing, and characteristics (e.g., perennial versus seasonal) await further study. A hiatus occurs at 193.13 mbsf, spanning a 25 m.y. interval from the early Miocene to the middle Eocene between ~18 Ma and 43 Ma. The sediment record during the middle Eocene is of dark, organic-rich biosiliceous composition. Isolated pebbles, interpreted as ice-rafted dropstones, are present down to 239 mbsf, well into this middle Eocene interval. Around the lower/middle Eocene boundary an abundance of Azolla spp. occurs, suggesting that a fresh and/or low-salinity surface water setting dominated the region during this time period. Although predrilling predictions based on geophysical data had placed the base of the sediment column at 50 Ma, drilling revealed that the uppermost Paleocene to lowermost Eocene boundary interval, well known as the Paleocene/Eocene Thermal Maximum (PETM), was recovered. During the PETM, the temperature of the Arctic Ocean surface waters exceeded 20°C.Drilling during Expedition 302 also penetrated into the underlying sedimentary bedrock, revealing a shallow-water depositional environment of Late Cretaceous age

Methods

Information assembled in this chapter will help the reader understand the basis for the preliminary conclusions of the Expedition 302 Scientists and will also enable the interested investigator to select samples for further analyses. This information concerns offshore and onshore operations and analyses described in the "Sites M0001–M0004" chapter. Methods used by various investigators for shore-based analyses of Expedition 302 samples will be described in the individual contributions published in the Expedition Research Results and in various professional journals