Reimagining the General Health Questionnaire as a measure of emotional wellbeing: A study of postpartum women in Malta

Background: Postpartum health has been subject to a focus on psychological morbidity, despite positive associations between postpartum recovery and maternal emotional wellbeing. There are currently many validated tools to measure wellbeing and related concepts, including non-psychiatric morbidity. The General Health Questionnaire, 12 items (GHQ-12) is one such instrument, widely used and validated in several languages. Its use in postpartum settings has been documented with disagreement about the instrument's utility in this population, particularly in relation to scoring method and threshold. The GHQ-12 has never been translated into Maltese. This study explored the psychometric properties of the GHQ-12 in a Maltese postpartum population to consider if the use of a different scoring method (visual analogue scale) in the GHQ-12 can determine postpartum wellbeing. Methods: One hundred and twenty-four postpartum women recruited from one hospital in Malta completed the translated and adapted GHQ-12 as a wellbeing measure (GHQ-12(WB)) at four postpartum time points. The psychometric properties of the GHQ-12(WB) were explored using confirmatory factor analysis, discriminant and divergent validity and reliability analysis. Results: The GHQ-12(WB) demonstrated good divergent and known-groups validity and internal consistency. No models offered a good fit to the data. The overall consistent best-fit to the data was an eight item, two factor model (GHQ-8). Model fit improved across all models in terms of CFI at 13 weeks. Conclusion: Findings generally support the reliability and validity of the Maltese version of the GHQ-12(WB). Model fit changes over time reflect the dynamic nature of postpartum recovery. Further evaluation of the GHQ-8(WB) is recommended. © 2013 Australian College of Midwives

How linear features alter predator movement and the functional\ud response

In areas of oil and gas exploration, seismic lines have been reported to alter the movement patterns of wolves (Canis lupus). We developed a mechanistic first passage time model, based on an anisotropic elliptic partial differential equation, and used this to explore how wolf movement responses to seismic lines influence the encounter rate of the wolves with their prey. The model was parametrized using 5 min GPS location data. These data showed that wolves travelled faster on seismic lines and had a higher probability of staying on a seismic line once they were on it. We simulated wolf movement on a range of seismic line densities and drew implications for the rate of predator–prey interactions as described by the functional response. The functional response exhibited a more than linear increase with respect to prey density (type III) as well as interactions with seismic line density. Encounter rates were significantly higher in landscapes with high seismic line density and were most pronounced at low prey densities. This suggests that prey at low population densities are at higher risk in environments with a high seismic line density unless they learn to avoid them

Relationship between pre-season testing performance and playing time among NCAA DII basketball players

Purpose: The purpose of this study was to investigate the relationships between pre-season testing performance and playing time within a men’s Division II basketball team. Methods: Archival data from pre-season athletic performance testing for ten (n=10) male NCAA Division II basketball players was collected and analyzed to determine if there was a relationship between anthropometric data (height, weight, wingspan), physical performance tests (vertical jump height, lane agility test, 5 and 20 m sprint time, National Basketball League (NBA) line drill and 20 m multi-stage fitness test (MSFT)), and playing time in the subsequent collegiate season. Results: Pearson’s product moment correlations revealed significant correlations were observed between playing time and predicted 1-RM bench press (r≥0.71) and 1-RM back squat (r≥0.74). Conclusion: These results reveal the importance of upper and lower body strength to determine playing time for Division II basketball players. Based on these results, coaches should emphasize the importance of resistance training to develop upper and lower body strength to increase playing time in Division II collegiate athletes

Fully-automated identification of imaging biomarkers for post-operative cerebellar mutism syndrome using longitudinal paediatric MRI

Post-operative cerebellar mutism syndrome (POPCMS) in children is a post- surgical complication which occurs following the resection of tumors within the brain stem and cerebellum. High resolution brain magnetic resonance (MR) images acquired at multiple time points across a patient’s treatment allow the quantification of localized changes caused by the progression of this syndrome. However, MR images are not necessarily acquired at regular intervals throughout treatment and are often not volumetric. This restricts the analysis to 2D space and causes difficulty in intra- and inter-subject comparison. To address these challenges, we have developed an automated image processing and analysis pipeline. Multi-slice 2D MR image slices are interpolated in space and time to produce a 4D volumetric MR image dataset providing a longitudinal representation of the cerebellum and brain stem at specific time points across treatment. The deformations within the brain over time are represented using a novel metric known as the Jacobian of deformations determinant. This metric, together with the changing grey-level intensity of areas within the brain over time, are analyzed using machine learning techniques in order to identify biomarkers that correspond with the development of POPCMS following tumor resection. This study makes use of a fully automated approach which is not hypothesis-driven. As a result, we were able to automatically detect six potential biomarkers that are related to the development of POPCMS following tumor resection in the posterior fossa

Culture of airway epithelial cells from neonates sampled within 48-hours of birth

Peer reviewedPublisher PD

Solution structures of human myeloma IgG3 antibody reveal extended Fab and Fc regions relative to the other IgG subclasses

Human immunoglobulin IgG3 possesses a uniquely long hinge region that separates its Fab antigen-binding and Fc receptor-binding regions. Owing to this hinge length, the molecular structure of full-length IgG3 remains elusive, and the role of the two conserved glycosylation sites in the Fc region is unknown. To address these issues, we subjected glycosylated and deglycosylated human myeloma IgG3 to multidisciplinary solution structure studies. Using analytical ultracentrifugation, the elongated structure of IgG3 was determined from the reduced sedimentation coefficients s020,w of 5.82-6.29 S for both glycosylated and deglycosylated IgG3. X-ray and neutron scattering showed that the Guinier RG values were 6.95 nm for glycosylated IgG3 and were unchanged after deglycosylation, again indicating an elongated structure. The distance distribution function P(r) of both forms of IgG3 showed a maximum length of 25-28 nm and three distinct maxima. The molecular structure of IgG3 was determined using atomistic modelling based on molecular dynamics simulations of the IgG3 hinge and Monte Carlo simulations to identify physically-realistic arrangements of the Fab and Fc regions. This resulted in libraries containing 135,135 and 73,905 glycosylated and deglycosylated IgG3 structures respectively. Comparisons with the X-ray and neutron scattering curves gave 100 best-fit models for each of the two forms of IgG3 that accounted for the experimental scattering curves. These models revealed the first molecular structures for full-length IgG3. The structures exhibited relatively-restricted Fab and Fc conformations joined by an extended semi-rigid hinge, which explains the potent effector functions of IgG3 relative to the other subclasses IgG1, IgG2 and IgG4

What do older people learn from young people? : Intergenerational learning in ‘day centre’ community settings in Malta

This study analyses what motivates older people to attend ‘day centres’ in Malta and what they believe that they derive from young people who carry out their placements at these day ‘centres’ These young people, who are aged 16–17, attend a vocational college in Malta and are studying health and social care. The study is based on a qualitative approach and employs the usage of focus groups. The main findings are that the elderly see the students as helping them on an emotional level by giving them encouragement, and on a practical level, by offering them insights that help them in modern-day life

A simple, efficient, mass-conservative approach to solving Richards' equation (openRE, v1.0)

A simple numerical solution procedure – namely the method of lines combined with an off-the-shelf ordinary differential equation (ODE) solver – was shown in previous work to provide efficient, mass-conservative solutions to the pressure-head form of Richards' equation. We implement such a solution in our model openRE. We developed a novel method to quantify the boundary fluxes that reduce water balance errors without negative impacts on model runtimes – the solver flux output method (SFOM). We compare this solution with alternatives, including the classic modified Picard iteration method and the Hydrus 1D model. We reproduce a set of benchmark solutions with all models. We find that Celia's solution has the best water balance, but it can incur significant truncation errors in the simulated boundary fluxes, depending on the time steps used. Our solution has comparable runtimes to Hydrus and better water balance performance (though both models have excellent water balance closure for all the problems we considered). Our solution can be implemented in an interpreted language, such as MATLAB or Python, making use of off-the-shelf ODE solvers. We evaluated alternative SciPy ODE solvers that are available in Python and make practical recommendations about the best way to implement them for Richards' equation. There are two advantages of our approach: (i) the code is concise, making it ideal for teaching purposes; and (ii) the method can be easily extended to represent alternative properties (e.g., novel ways to parameterize the K(ψ) relationship) and processes (e.g., it is straightforward to couple heat or solute transport), making it ideal for testing alternative hypotheses

Analysis of periosteal lesions from commingled human remains at the Xagħra Circle hypogeum reveals the first case of probable scurvy from Neolithic Malta

Abstract Objectives Palaeopathological analysis is key for characterising population health at the individual level and across large assemblages but is rarely exploited to unite the remains of disarticulated individuals. This study explores the potential for individual identification through differential diagnosis of periosteal lesions in a commingled deposit, both to ascertain the number of individuals represented and provide a differential diagnosis. Materials and Methods The late Neolithic Xag?ra Circle hypogeum on Gozo contains the remains of more than 800 individuals, most of which were transformed to a collective disarticulated assemblage. Across the excavated population, pathological observations are strikingly low. In one specific 1???1-m area in a single stratigraphic context, fragmented and disarticulated cranial and post-cranial non-adult bones were identified that displayed periosteal new bone formation. To aid differential diagnosis, macroscopic analysis, taphonomic analysis and micro-computed tomography (?CT) imaging were integrated. Results This approach, when combined with osteobiographical analyses, reveals that the elements most likely derive from one individual, a young child, who presents a probable case of scurvy. The potential for micronutrient co-morbidities are explored, but without further microscopic study it cannot be determined if this individual also experienced iron-deficiency anaemia and/or rickets. Discussion In the context of the Mediterranean and Europe in later prehistory, reported cases of scurvy are currently low and often reveal periods of environmental instability and resource insufficiency. Our finding of non-adult scurvy in late 3rd millennium BC Malta contributes to a developing picture of an increasingly unstable palaeoenvironment and declining population health at this time, although it may also indicate an individual case of poor childhood health within this broader context.1 Introduction 1.1 Archaeological context of the Xagħra Circle hypogeum 2 Materials and methods 2.1 Remains presenting periosteal lesions 2.2 Macroscopic and micro-CT analysis 3 Macroscopic and radiological observations of pathology 3.1 Frontal bone (FB0039, FB0040) and zygoma (FB0041) 3.2 Mandible (FB0042) 3.3 Left rib (FB0043) and right rib (FB0044) 3.4 Ulna (FB0045) 4 Differential diagnosis 4.1 Frontal bone (FB0039, FB0040) and zygoma (FB0041) 4.2 Mandible (FB0042) 4.3 Left rib (FB0043) and right rib (FB0044) 4.4 Ulna (FB0045) 5 Discussion 5.1 Scurvy: Causes, consequences and comorbidities 5.2 Scurvy in prehistory 5.3 The Maltese context 6 Conclusio