Ecological features of Terebellida fauna (Annelida, Polychaeta) from Ensenada de San Simón (NW Spain)

Ecological features of Terebellida (Annelida, Polychaeta) inhabiting the intertidal and subtidal soft-bottoms of Ensenada de San Simón (NW Spain) were analysed by means of quantitative sampling. A total of 4,814 specimens belonging to five families (Ampharetidae, Pectinariidae, Terebellidae, Trichobranchidae and Sabellariidae) and ten species were collected in a variety of substrata and depths. Ampharetidae was the numerically dominant family mostly due to the abundance of Ampharete finmarchica and Melinna palmata; these species accounted for up to 94% of the total Terebellida abundance. Intertidal areas colonised by the seagrasses Zostera marina L. and Z. noltii Hornem. One thousand eight hundred and thirty-two harboured low densities of Terebellida, whereas the deeper subtidal muddy bottoms showed high abundances of ampharetids. Multivariate analyses suggested that Terebellida assemblages are highly correlated with sediment composition

Evaluation of the changes in working limits in an automobile assembly line using simulation

The aim of the work presented in this paper consists of the development of a decision-making support system, based on discrete-event simulation models, of an automobile assembly line which was implemented within an Arena simulation environment and focused at a very specific class of production lines with a four closed-loop network configuration. This layout system reflects one of the most common configurations of automobile assembly and preassembly lines formed by conveyors. The sum of the number of pallets on the intermediate buffers, remains constant, except for the fourth closed-loop, which depends on the four-door car ratio (x) implemented between the door disassembly and assembly stations of the car body. Some governing equations of the four closed-loops are not compatible with the capacities of several intermediate buffers for certain values of variable x. This incompatibility shows how the assembly line cannot operate in practice for x0,97 in a stationary regime, due to the starvation phenomenon or the failure of supply to the machines on the production line. We have evaluated the impact of the pallet numbers circulating on the first closed-loop on the performance of the production line, translated into the number of cars produced/hour, in order to improve the availability of the entire manufacturing system for any value of x. Until the present date, these facts have not been presented in specialized literature. © 2012 American Institute of Physics

Variable effect of 3 different chondroitin sulfate compounds on chondrocytes secreted proteins profile revealed by silac techinique

Comunicaciones a congreso

Estudio comparativo del secretoma de condrocitos articulares humanos analizados mediante las técnicas iTRAQ e SILAC

Comunicaciones a congreso

Cuantificación por SILAC del efecto de la nicotina sobre condrocitos articulares

Comunicaciones a congreso

Quantitative proteomics analysis of chondrogenic differentiation of mesenchymal stem cells by SILAC

Comunicaciones a congreso

An atlas of the knee joint proteins and their role in osteoarthritis defined by literature mining

[Abstract] Osteoarthritis (OA) is the most prevalent rheumatic pathology. However, OA is not simply a process of wear and tear affecting articular cartilage but rather a disease of the entire joint. One of the most common locations of OA is the knee. Knee tissues have been studied using molecular strategies, generating a large amount of complex data. As one of the goals of the Rheumatic and Autoimmune Diseases initiative of the Human Proteome Project, we applied a text-mining strategy to publicly available literature to collect relevant information and generate a systematically organized overview of the proteins most closely related to the different knee components. To this end, the PubPular literature-mining software was employed to identify protein-topic relationships and extract the most frequently cited proteins associated with the different knee joint components and OA. The text-mining approach searched over eight million articles in PubMed up to November 2022. Proteins associated with the six most representative knee components (articular cartilage, subchondral bone, synovial membrane, synovial fluid, meniscus, and cruciate ligament) were retrieved and ranked by their relevance to the tissue and OA. Gene ontology analyses showed the biological functions of these proteins. This study provided a systematic and prioritized description of knee-component proteins most frequently cited as associated with OA. The study also explored the relationship of these proteins to OA and identified the processes most relevant to proper knee function and OA pathophysiology.PI19/01206; Instituto de Salud Carlos IIIPI20/00793; Instituto de Salud Carlos IIIPI20/01409; Instituto de Salud Carlos IIIPI22/01155; Instituto de Salud Carlos IIIRICORS-REIRD21/0002/0009; Instituto de Salud Carlos IIIED431E2018/03; Xunta de GaliciaIN607A2021/07; Xunta de GaliciaIN607D2020/10; Xunta de Galici

Secretome analysis of chondroitin sulfate-treated chondrocytes reveals anti-angiogenic, anti-inflammatory and anti-catabolic properties

Introduction: Chondroitin sulfate (CS) is a symptomatic slow-acting drug for osteoarthritis (OA) widely used in the clinic. The aim of this work is to find proteins whose secretion from cartilage cells under proinflammatory stimuli (IL-1β) is regulated by CS, employing a novel quantitative proteomic approach. Methods: Human articular chondrocytes released from three normal cartilages were grown in SILAC medium. When complete incorporation of the heavy isotope was achieved, chondrocytes were stimulated with IL-1β 5 ng/ml with or without CS pretreatment (200 µg/ml). Forty-eight hours later, chondrocyte secretomes were analyzed by nano-scale liquid chromatography-mass spectrometry. Real-time PCR, western blot and immunohistochemistry analyses were employed to confirm some of the results. Results: We could identify 75 different proteins in the secretome of human articular chondrocytes. Eighteen of these were modulated by CS with statistical significance (six increased and 12 decreased). In normal chondrocytes stimulated with IL-1β, CS reduces inflammation directly by decreasing the presence of several complement components (CFAB, C1S, CO3, and C1R) and also indirectly by increasing proteins such as TNFα-induced protein (TSG6). TSG6 overexpression correlates with a decrease in pro-matrix metalloproteinase activation (observed in MMP1 and MMP3 levels). Finally, we observed a strong CS-dependent increase of an angiogenesis inhibitor, thrombospondin-1. Conclusion: We have generated a quantitative profile of chondrocyte extracellular protein changes driven by CS in the presence of IL-1β. We have also provided novel evidences of its anti-angiogenic, anti-inflammatory, and anti-catabolic properties. Demonstration of the anti-angiogenic action of CS might provide a novel therapeutic approach for OA targeting

Infaunal macrobenthos in the Porcupine Bank (NE Atlantic)

The results of the macroinfaunal communities distributions from the annual series surveys PORCUPINE are shown. Those surveys were realized during the months of September and October in the years 2006, 2008, 2009, 2010, 2011, 2012 and 2013 by the Instituto Español de Oceanografía aboard the R /V Vizconde de Eza, within the research project ERDEM. This survey series has as main objective the obtainment of abundance indexes of the benthonic and demersal fauna from Porcupine Bank, west of Ireland, with special attention on the commercial species exploited by the Spanish fleet (hake, angler fish, megrim and norwegian lobster). For the study of the infaunal macrobenthos a total of 50 samples were analyzed from selected places of the Bank, where the bottom characteristics, the climatology or the survey schedule allowed. Samples were taken with an ULSNER box corer with 0.09 m2 of sampling area. Samples for the faunal study were sieved aboard with a 0.5 mm mesh size sieve. The material collected in the sieve was preserved with 8 % formaldehyde neutralized with borax, and stained with Rose Bengal solution for the posterior sorting and identification of fauna. Additional samples were taken to estimate the organic content of the sediment and to perform the granulometric analyses. Once in the laboratory, the granulometry was analyzed following the method recommended by Buchanan (1984); which consists in a combination of dry sieving of the coarse fraction (> 62 µm) and the sedimentation at 20 ºC of the fine fraction (< 62 µm) in a distilled water column. Sediment organic matter content was calculated as the percentage of weight loss by means of 24 hours sample calcination at 500 ºC, after dried it at 100 ºC during the same time. Complementary, in order to obtain a better coverage of the sedimentary types from the study area, more than 350 samples were collected with a net collector coupled to the trawling fishing gear (Baca-GAV 39/52). Globally, the sediments of the majority of the sampled stations are mainly formed by sand, between fine and very fine, except in deep areas of the southeast, where there are large muddy areas with elevated organic content and high selection coefficient. In the north, around the shallowest area, we found thicker sandy sediments, with lower organic content and moderate selection. The macroinfaunal communities from the Porcupine Bank are characterized by the dominance of the faunistic group of polychaetes (68.8 %), mainly represented by the families Owenidae, Spionidae, Paraonidae and Sabellidae. The next in number are the group of molluscs (17.6 %), dominated by the family Kelliellidae, which reaches maximum abundances up to 7520 ind•m-2. The poorest represented groups are the crustaceans (5.0 %), echinoderms (4.3 %) and the “others” (sipunculids, nemertine, etc.), being the latter the least abundant (3.7 %). In relation with the spatial distribution four macroinfaunal groups were indentified in the Porcupine Bank: - Group 1: located in intermediate depth areas, over sandy sediments with low organic content. The infaunal community is dominated by polychaetes from the Owenidae family, mainly represented by the specie Galathowenia oculata. - Group 2: occupies the sandy and low organic content sediments from the shallowest area of the Bank. Polychaetes are the most representative faunistic group, highlighting the dominance of the families Spionidae and Paraonidae. Prionospio fallax and Aricidea wassi are the most representative species of the group. - Group 3: situated in two deep areas in the south area of the Bank with muddy and high organic matter content sediments. It has the lowest infaunal abundance of the study, being dominated by the bivalve molluscs from the family Kelliellidae and by the annelids polychaetes from the family Paraonidae. - Group 4: Is the cluster that includes more stations in throughout the study. It is distributed spatially in the south area of the Bank, in different bathymetries, with the most heterogeneous sediments and moderate organic content. The bivalve molluscs from the Kelliellidae family again become dominant, with Kelliella miliaris reaching abundance over 7500 ind•m-2.IE