Assessment of motor coordination in primary education of Barcelona and province

El presente estudio tiene por objetivo valorar la coordinación motora de estudiantes de primaria de Barcelona y provincia. Para la evaluación se ha utilizado el test KTK en el que participaron 1254 personas, 670 niños y 584 niñas, de edades entre 7 y 10 años. Se realizó el análisis descriptivo y la comparación entre género y edad. Los resultados indican que más del 40% de la población estudiada presenta una coordinación por debajo de la normalidad, cerca de un 57% de la muestra fue clasificado con coordinación normal y solamente el 4,6% lo ha sido por encima de esta clasificación. Los chicos han presentado resultados significativamente mejores que las chicas. Los datos del estudio no permiten generalizar los resultados, sin embargo como es una muestra representativa, nos lleva a creer que la población de alumnos en educación primaria de Barcelona y su provincia tiene un nivel coordinativo por debajo del esperado para su edadThe present study aims to assess motor coordination of primary-school students of Barcelona and its province. For evaluation we used the KTK test. 1254 people, 670 boys and 584 girls with ages between 7 and 10 years participated in the study. Descriptive analysis and the comparison between gender and age were performed. The results indicate that about 40% of the population studied presents results below normal, around 57% were classified with normal coordination and only 4.6% has been above this rating. The boys have presented significantly better results than girls. The study data do not permit to generalize the results, however as a representative sample, it leads us to believe that the student population in elementary education from Barcelona and its province has a coordinative level below expected for their ageAgrupació de Recerca en Ciències de l’Educació. U

Características de una web-App educativa de EF en CLIL para el profesorado

La presencia de las web-Apps que se denominan educativas crece a gran velocidad; en la actualidad hay disponibles más de 500.000 en iOS y Android. Sin embargo, muchas de ellas no cumplen los requisitos necesarios para ser consideradas educativas, tales como aspectos técnicos, estéticos, didácticos y pedagógicos. El objetivo del estudio es determinar las características a las que una web-App de educación física en CLIL para el profesorado debería ajustarse para considerarse educativa. La metodología utilizada para esta investigación es el análisis de contenidos de las características de diseño y educativas de 31 web-Apps dirigidas al profesorado que se encuentran entre las más utilizadas y mejor valoradas. En el procedimiento se han establecido una serie de dimensiones y categorías que nos han permitido identificar si las diferentes web-Apps seleccionadas en las que se puede crear y compartir material educativo, disponen de las características pertinentes tanto de diseño como educativas. Los resultados obtenidos, nos han permitido determinar cuáles son las características más comunes entre las aplicaciones web de este tipo y presentar una propuesta de cuáles deberían ser las de una web-App EF en CLIL para el profesorado

Diseño del prototipo de una web-App de Educación Física en Content and Language Integrated Learning (CLIL)

El presente estudio constituye la segunda fase (II) de una investigación que pretende diseñar, implementar y evaluar una web-App para la enseñanza aprendizaje de la educación física (EF) en CLIL. El estudio tiene como objetivo diseñar el prototipo de la web-App a través del análisis de web-Apps vigentes relacionadas con la creación y compartición de materiales educativos, la descripción de los indicadores para la personalización del contenido, la selección de las herramientas tecnológicas más adecuadas y lograr su construcción aplicando los aspectos pedagógicos y tecnológicos más pertinentes. Para la elaboración de la web-App y el cumplimiento de los objetivos planteados se ha utilizado la metodología de la investigación basada en el diseño (IBD), con la voluntad de introducir un elemento tecnológico transformador que dé respuesta a las necesidades del profesorado. El resultado ha sido la creación de una plataforma donde los usuarios puedan crear, compartir y explorar sesiones de EF en CLIL que disponen de los elementos clave para su éxito

Effect of monovalency on anti-contactin-1 IgG4

Altres ajuts: Agence Nationale pour le Développement de la Recherche en Santé ; Association Française contre les Myopathies ; ArgenxAutoimmune nodopathies (AN) have been diagnosed in a subset of patients fulfilling criteria for chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) who display no or poor response to intravenous immunoglobulins. Biomarkers of AN are autoantibodies, mainly IgG4, directed against the ternary paranodal complex composed by neurofascin-155, contactin-1 (CNTN1), and Contactin-associated-protein-1 (CASPR1) or against the nodal isoforms of neurofascin. IgG4 can undergo a Fab-arm exchange (FAE) which results in functionally monovalent antibody. This phenomenon differentially affects the pathogenicity of IgG4 depending on the target of autoantibodies. Here, we have evaluated this issue by examining the impact of valency on anti-CNTN1 IgG4 which induces paranodal destruction through a function blocking activity. Sera were obtained from 20 patients with AN associated with anti-CNTN1 antibodies. The proportion of monospecific/bispecific anti-CNTN1 antibodies was estimated in each patient by ELISA by examining the ability of serum antibodies to cross-link untagged CNTN1 with biotinylated CNTN1. To determine the impact of monovalency, anti-CNTN1 IgG4 were enzymatically digested into monovalent Fab and tested in vitro on cell aggregation assay. Also, intraneural injections were performed to determine whether monovalent Fab and native IgG4 may penetrate paranode, and antibody infiltration was monitored 1- and 3-days post injection. We found that the percentage of monospecific antibodies were lower than 5% in 14 out of 20 patients (70%), suggesting that IgG4 have undergone extensive FAE in situ. The levels of monospecific antibodies correlated with the titers of anti-CNTN1 antibodies. However, no correlation was found with clinical severity, and patients with low or high percentage of monospecific antibodies similarly showed a severe phenotype. Native anti-CNTN1 IgG4 were shown to inhibit the interaction between cells expressing CNTN1/CASPR1 and cells expressing neurofascin-155 using an in vitro aggregation assay. Similarly, monovalent Fab significantly inhibited the interaction between CNTN1/CASPR1 and neurofascin-155. Intraneural injections of Fab and native anti-CNTN1 IgG4 indicated that both mono- and bivalent anti-CNTN1 IgG4 potently penetrated the paranodal regions and completely invaded this region by day 3. Altogether, these data indicate anti-CNTN1 IgG4 are mostly bispecific in patients, and that functionally monovalent anti-CNTN1 antibodies have the pathogenic potency to alter paranode

Hacia una educación física comprometida con la sociedad

La educación física dirigida al desarrollo de la persona y su socialización, así como a la promoción de una vida saludable debería asegurar una relación y compromiso directo con el contexto social. Con este objetivo, se presentan diferentes propuestas de ApS que han sido realizadas con éxito en diferentes etapas educativas

Serum Contactin-1 in CIDP

To investigate whether serum levels of contactin-1, a paranodal protein, correlate with paranodal injury as seen in patients with CIDP with antibodies targeting the paranodal region. Serum contactin-1 levels were measured in 187 patients with CIDP and 222 healthy controls. Paranodal antibodies were investigated in all patients. Serum contactin-1 levels were lower in patients (N = 41) with paranodal antibodies compared with patients (N = 146) without paranodal antibodies (p < 0.01) and showed good discrimination between these groups (area under the curve 0.84; 95% CI: 0.76-0.93). These findings suggest that serum contactin-1 levels have the potential to serve as a possible diagnostic biomarker of paranodal injury in CIDP. This study provides class II evidence that serum contactin-1 levels can discriminate between patients with CIDP with or without paranodal antibodies with a sensitivity of 71% (95% CI: 56%-85%) and a specificity of 97% (95% CI: 83%-100%)

Isolation of human fibroadipogenic progenitors and satellite cells from frozen muscle biopsies

Altres ajuts: Association Française contre les Myopathies (22525)Altres ajuts: Fundación Isabel GemioSkeletal muscle contains multiple cell types that work together to maintain tissue homeostasis. Among these, satellite cells (SC) and fibroadipogenic progenitors cells (FAPs) are the two main stem cell pools. Studies of these cells using animal models have shown the importance of interactions between these cells in repair of healthy muscle, and degeneration of dystrophic muscle. Due to the unavailability of fresh patient muscle biopsies, similar analysis of interactions between human FAPs and SCs is limited especially among the muscular dystrophy patients. To address this issue here we describe a method that allows the use of frozen human skeletal muscle biopsies to simultaneously isolate and grow SCs and FAPs from healthy or dystrophic patients. We show that while the purified SCs differentiate into mature myotubes, purified FAPs can differentiate into adipocytes or fibroblasts demonstrating their multipotency. We find that these FAPs can be immortalized and the immortalized FAPs (iFAPs) retain their multipotency. These approaches open the door for carrying out personalized analysis of patient FAPs and interactions with the SCs that lead to muscle loss

Autoantibody screening in Guillain-Barré syndrome

Background: Guillain-Barré syndrome (GBS) is an acute inflammatory neuropathy with a heterogeneous presentation. Although some evidences support the role of autoantibodies in its pathogenesis, the target antigens remain unknown in a substantial proportion of GBS patients. The objective of this study is to screen for autoantibodies targeting peripheral nerve components in Guillain-Barré syndrome. Methods: Autoantibody screening was performed in serum samples from all GBS patients included in the International GBS Outcome study by 11 different Spanish centres. The screening included testing for anti-ganglioside antibodies, anti-nodo/paranodal antibodies, immunocytochemistry on neuroblastoma-derived human motor neurons and murine dorsal root ganglia (DRG) neurons, and immunohistochemistry on monkey peripheral nerve sections. We analysed the staining patterns of patients and controls. The prognostic value of anti-ganglioside antibodies was also analysed. Results: None of the GBS patients (n = 100) reacted against the nodo/paranodal proteins tested, and 61 (61%) were positive for, at least, one anti-ganglioside antibody. GBS sera reacted strongly against DRG neurons more frequently than controls both with IgG (6% vs 0%; p = 0.03) and IgM (11% vs 2.2%; p = 0.02) immunodetection. No differences were observed in the proportion of patients reacting against neuroblastoma-derived human motor neurons. Reactivity against monkey nerve tissue was frequently detected both in patients and controls, but specific patterns were only detected in GBS patients: IgG from 13 (13%) patients reacted strongly against Schwann cells. Finally, we confirmed that IgG anti-GM1 antibodies are associated with poorer outcomes independently of other known prognostic factors. Conclusion: Our study confirms that (1) GBS patients display a heterogeneous repertoire of autoantibodies targeting nerve cells and structures; (2) gangliosides are the most frequent antigens in GBS patients and have a prognostic value; (3) further antigen-discovery experiments may elucidate other potential antigens in GBS