12 research outputs found
Erratum to: Burkholderia pseudomallei induces IL-23 production in primary human monocytes.
The original version of this article unfortunately contained a mistake. The labelling of Fig. 1 was incorrect
RNA-seq reveals infection-induced gene expression changes in the snail intermediate host of the carcinogenic liver fluke, Opisthorchis viverrini
Background: Bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, Opisthorchis viverrini, the leading cause of cholangiocarcinoma (CCA) in the Greater Mekong sub-region of Thailand. Despite the severe public health impact of Opisthorchis-induced CCA, knowledge of the molecular interactions occurring between the parasite and its snail intermediate host is scant. The examination of differences in gene expression profiling between uninfected and O. viverrini-infected B. siamensis goniomphalos could provide clues on fundamental pathways involved in the regulation of snail-parasite interplay.\ud
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Methodology/Principal Findings:Using high-throughput (Illumina) sequencing and extensive bioinformatic analyses, we characterized the transcriptomes of uninfected and O. viverrini-infected B. siamensis goniomphalos. Comparative analyses of gene expression profiling allowed the identification of 7,655 differentially expressed genes (DEGs), associated to 43 distinct biological pathways, including pathways associated with immune defense mechanisms against parasites. Amongst the DEGs with immune functions, transcripts encoding distinct proteases displayed the highest down-regulation in Bithynia specimens infected by O. viverrini; conversely, transcription of genes encoding heat-shock proteins and actins was significantly up-regulated in parasite-infected snails when compared to the uninfected counterparts.\ud
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Conclusions/Significance:The present study lays the foundation for functional studies of genes and gene products potentially involved in immune-molecular mechanisms implicated in the ability of the parasite to successfully colonize its snail intermediate host. The annotated dataset provided herein represents a ready-to-use molecular resource for the discovery of molecular pathways underlying susceptibility and resistance mechanisms of B. siamensis goniomphalos to O. viverrini and for comparative analyses with pulmonate snail intermediate hosts of other platyhelminths including schistosomes
Erratum to: Burkholderia pseudomallei induces IL-23 production in primary human monocytes
Genetic variation of a freshwater snail Hydrobioides nassa (Gastropoda: Bithyniidae) in Thailand examined by mitochondrial DNA sequences
The top ten up-regulated genes linked to biological pathways associated to immune responses [based on orthology to molecules in the Kyoto Encyclopedia of Genes and Genomes (KEGG)] in each uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively).
<p>The top ten up-regulated genes linked to biological pathways associated to immune responses [based on orthology to molecules in the Kyoto Encyclopedia of Genes and Genomes (KEGG)] in each uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively).</p
(a) Enriched Gene Ontology (GO) terms assigned to differentially expressed genes (DEGs) in uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively), according to the categories ‘biological process’, ‘cellular component’ and molecular function; (b) enriched KEGG biological pathways assigned to DEGs in UB and IB, respectively.
<p>(a) Enriched Gene Ontology (GO) terms assigned to differentially expressed genes (DEGs) in uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively), according to the categories ‘biological process’, ‘cellular component’ and molecular function; (b) enriched KEGG biological pathways assigned to DEGs in UB and IB, respectively.</p
Scatter plot comparing log2 ratios of FPKM expression values for uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively).
<p>Scatter plot comparing log2 ratios of FPKM expression values for uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively).</p
The top-ten up-regulated genes in uninfected and <i>Opisthorchis viverrini</i>-infected <i>Bithynia siamensis goniomphalos</i> snails (UB and IB, respectively).
<p>*non-redundant database.</p><p>**KEGG-Orthology based annotation definition.</p><p>***Based on comparisons with the preliminary <i>Biomphalaria glabrata</i> genome assembly v4.3 (<a href="http://biology.unm.edu/biomphalaria-genome/" target="_blank">http://biology.unm.edu/biomphalaria-genome/</a>).</p