Developmental competence and mRNA expression of preimplantation in vitro-produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1.

Recombinant human Insulin-like growth factor-I (hIGF-1) was administered to one ovary of prepubertal and postpubertal cattle to determine its effects on (1) oocyte developmental competence, (2) the expression pattern of six developmentally important genes (GLUT3, GLUT8, AKT1, BCL-XL, BAD, and BAX), and (3) its relationship with apoptosis (female Holstein-Friesian). Oocytes were retrieved from 7- to 10-mo-old prepubertal dairy calves (preP), 11- to 18-mo-old postpubertal heifers (postP), and cows via ultrasound-guided follicular aspiration. Immature oocytes were matured in vitro then fertilized and cultured up to the blastocyst stage. Apoptosis was determined by terminal deoxynucleotidyl transferase nick-end labeling (TUNEL) in 8-d blastocysts. Similar low blastocyst yields were observed in the IGF-1-treated preP group (11.2+/-2.4%), the control preP group (10.4+/-3.0%), and in the IGF-1 postP group (10.9+/-2.3%). These were lower (P<or=0.01) compared with the control postP group (21.2+/-3.8%) and with cows (23+/-3.7%). The expression profile of the six genes was partly affected by age and IGF-1 treatment. Apoptosis was correlated with the age of the oocyte donors and was increased in blastocysts derived from prepubertal heifers. Results show that apoptosis is a critical feature of the acquisition of developmental competence of oocytes from prepubertal cattle and that IGF-1 did not beneficially affect oocyte developmental competence

A Galerkin least-square stabilisation technique for hyperelastic biphasic soft tissue

An hyperelastic biphasic model is presented. For slow-draining problems (permeability less than 1 × 10−2 mm4 N−1 s−1), numerical instabilities in the form of non-physical oscillations in the pressure field are observed in 3D problems using tetrahedral Taylor–Hood finite elements. As an alternative to considerable mesh refinement, a Galerkin least-square stabilisation framework is proposed. This technique drastically reduces the pressure discrepancies and prevents these oscillations from propagating towards the centre of the medium. The performance and robustness of this technique are demonstrated on a 3D numerical example

Consolidation and dynamics of 3D unsaturated porous seabed under rigid caisson breakwater loaded by hydrostatic pressure and wave

In this study, based on the dynamic Biot’s theory “u-p” approximation, a 3D finite element method (FEM) numerical soil model is developed, in which the Generalized Newmark-method is adopted to determine the time integration. The developed 3D FEM soil model is a part of the coupled model PORO-WSSI 3D for 3D wave-seabed-marine structures interaction problem, and is validated by the analytical solution proposed by Wang (2000) for a laterally infinite seabed loaded by a uniform force. By adopting the developed 3D soil model, the consolidation of seabed under a caisson breakwater and hydrostatic pressure is investigated. The numerical results show that the caisson breakwater built on seabed has very significant effect on the stresses/displacements fields in the seabed foundation after the transient deformation and primary consolidation are completed. The parametric study indicates that the Young’s modulus E of seabed is the most important parameter to affect the settlement of breakwater, and the displacement fields in seabed foundation. Taking the consolidation status as the initial condition, the interaction between ocean wave, caisson breakwater and seabed foundation is briefly investigated. The 3D ocean wave is determined by solving the Navier-Stokes equations with finite volume method (FVM). The numerical results indicate that there is intensive interaction between ocean wave, caisson breakwater and seabed foundation; and the breakwater indeed can effectively block the wave energy propagating to the coastline