88 research outputs found
Biological characteristics of a cold-adapted influenza A virus mutation residing on a polymerase gene
The biological function of a cold-adapted (ca) mutation residing on the PB2 gene of an influenza A/Ann Arbor/6/60 (A/AA/6/60) ca variant virus in the viral replication cycle at 25° C was studied. The viral polypeptide synthesis of A/AA/6/60 ca variant at 25° C was evident approximately 6 hours earlier than the wild type (wt) virus and yielded twice as many products. The quantitative analysis of viral complementary RNA (cRNA), synthesized in the presence of cycloheximide, revealed that A/AA/6/60 ca variant and a single gene reassortant that contains only the PB2 gene of the ca variant with remaining genes of the wt virus produced equal amount of cRNA at 25° and 33° C, which was an amount approximately four fold greater than the wt virus' cRNA synthesized at 25° C. These results strongly suggest that the ca mutation residing on the PB2 gene of A/AA/6/60 ca variant affects the messenger RNA synthesis at 25° C in the primary transcription.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41692/1/705_2005_Article_BF01310893.pd
Uukuniemi Phlebovirus Assembly and Secretion Leave a Functional Imprint on the Virion Glycome
Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome
Ethnic differences in cathecol-O-methyltransferase pharmacogenetics: frequency of the codon 108 low activity allele is lower in Kenyan than Caucasian or Southwest Asian subjects
The autoimmune regulator: a key toward understanding the molecular pathogenesis of autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy
Paid informal care within Swedish eldercare: prevalence, change and variation among Swedish municipalities 2006–2016
Evidence for the presence of the minor capsid protein of Western equine encephalitis virus
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