Quantifying efficient shape-shifting: Energy barrier measurement in multi-stable lattice metamaterials

This is the final version. Available on open access from Elsevier via the DOI in this recordData availability: Data are available at the University of Bristol data repository, data.bris, at https://doi.org/10.5523/bris.1n7g692ynrlht214cxm7yk77qiShape-shifting between multiple stable deformation states offers attractive pathways to design adaptive structures. Ideas have been conceptualised in diverse fields, including soft robotics and aerospace engineering. The success of shape-shifting relies on overcoming the energy barrier separating adjacent stable configurations, which necessitates efficient actuation strategies. Recently, multistable mechanical metamaterials have been designed with shape-shifting controlled by an actuator at the local scale, i.e with embedded actuation. This local, embedded actuation creates challenges for quantifying the energy barriers required for shape-shifting. Specifically, the local actuation requires a pair of forces with opposite directions and the direction of the forces must remain constant throughout the entire loading process. Moreover, the loading points must move freely in a direction perpendicular to the loading direction. We present a novel bi-axial test rig for a typical multi-stable lattice metamaterial that accurately determines the energy barrier between stable states by using an embedded actuator and inducing shape-shifting. Our experimental design features two independent actuation systems operating at different length scales: a primary one for a globally applied axial compression of the metamaterial, and a secondary local system for triggering shape-shifting between different stable configurations. Experimental data obtained using this bespoke test rig unveil the metamaterial’s response to local, embedded actuation. Excellent agreement with finite element simulations is observed, demonstrating the effectiveness of the test setup in providing measurements of the energy barrier. This work provides a valuable benchmark for measuring energy barriers in multi-stable metamaterials and paves the way for rigorous validation and verification of novel functional metamaterial and structures that leverage shape-shifting mechanisms.Leverhulme TrustRoyal Academy of Engineering (RAE)Exeter Technologies Group, University of ExeterSwansea UniversityEuropean Research Council (ERC

Offshore Code Comparison Collaboration Continuation (OC4), Phase I – Results of Coupled Simulations of an Offshore Wind Turbine with Jacket Support Structure

Heike von Waaden (REpower Systems SE) To be presented at the 22 nd International Society of Offshore and Polar Engineers Conference Rhodes, Greec

Characterization and performance of nucleic acid nanoparticles combined with protamine and gold

Macromolecular nucleic acids such as DNA vaccines, siRNA, and splice-site switching oligomers (SSO) have vast chemotherapeutic potential. Nanoparticulate biomaterials hold promise for DNA and RNA delivery when a means for binding is identified that retains structure-function and provides stabilization by the nanoparticles. In order to provide these benefits of binding, we combined DNA and RNA with protamine— demonstrating association to gold microparticles by electrophoretic, gel shot, fluorescence, and dynamic laser light spectroscopy (DLLS). A pivotal finding in these studies is that the Au-protamine-DNA conjugates greatly stabilize the DNA; and DNA structure and vaccine activity are maintained even after exposure to physical, chemical, and temperature-accelerated degradation. Specifically, protamine formed nanoparticles when complexed to RNA. These complexes could be detected by gel shift and were probed by high throughput absorbance difference spectroscopy (HTADS). Biological activity of these RNA nanoparticles (RNPs) was demonstrated also by a human tumor cell splice-site switching assay and by siRNA delivery against B-Raf—a key cancer target. Finally, RNA:protamine particles inhibited growth of cultured human tumor cells and bacteria. These data provide new insights into DNA and RNA nanoparticles and prospects for their delivery and chemotherapeutic activity

Correlation of magnetic field intensities and solar wind speeds of events observed by ACE

The relationship between the magnetic field intensity and speed of solar wind events is examined using ∼3 years of data from the ACE spacecraft. No preselection of coronal mass ejections (CMEs) or magnetic clouds is carried out. The correlation between the field intensity and maximum speed is shown to increase significantly when |B| > 18 nT for 3 hours or more. Of the 24 events satisfying this criterion, 50% are magnetic clouds, the remaining half having no ordered field structure. A weaker correlation also exists between southward magnetic field and speed. Sixteen of the events are associated with halo CMEs leaving the Sun 2 to 4 days prior to the leading edge of the events arriving at ACE. Events selected by speed thresholds show no significant correlation, suggesting different relations between field intensity and speed for fast solar wind streams and ICMEs

Disability in young adults following major trauma: 5 year follow up of survivors

BACKGROUND: Injuries are a major cause of mortality and morbidity in young people. Despite this, the long-term consequences for young survivors of severe injury are relatively unexplored. METHODS: Population based cohort study involving 5 year post injury structured interview of all cases of major trauma (Injury Severity Score > 15) identified retrospectively for 12 month period (1988 to 1989) within former Yorkshire Health Authority area of the United Kingdom. RESULTS: 125 individuals aged 11–24 years at time of injury were identified. Of these, 109 (87%) were interviewed. Only 20% (95% CI 14–29%) of those interviewed reported no disability. Mean Office of Population Census and Surveys (OPCS) disability score of the remainder was 7.5 (median 5.8, range 0.5 to 19.4). The most commonly encountered areas of disability were behaviour (54%, 95% CI 45–63%), intellectual functioning (39%, 95% CI 31–49%) and locomotion (29%, 95% CI 22–39%). Many respondents reported that their daily lives were adversely affected by their health problems for example, causing problems with work, 54% (95% CI 45–63%), or looking after the home, 28% (95% CI 21–38%). Higher OPCS scores were usually but not always associated with greater impact on daily activities. The burden of caring responsibilities fell largely on informal carers. 51% (95% CI 42–61%) of those interviewed would have liked additional help to cope with their injury and disability. CONCLUSION: The study has revealed significant disability amongst a cohort of young people 5 years post severe injury. Whilst many of these young people were coping well with the consequences of their injuries, others reported continuing problems with the activities of daily life. The factors underpinning the young people's differing experiences and social outcome should be explored

Characterization of biomolecular nanoconjugates by high-throughput delivery and spectroscopic difference

Nanoparticle conjugates have the potential for delivering siRNA, splice-shifting oligomers or nucleic acid vaccines, and can be applicable to anticancer therapeutics. This article compares tripartite conjugates with gold nanoparticles or synthetic methoxypoly(ethylene glycol)-block-polyamidoamine dendrimers