Predicting aboveground forest biomass with topographic variables in human-impacted tropical dry forest landscapes

Topographic variables such as slope and elevation partially explain spatial variations in aboveground biomass (AGB) within landscapes. Human activities that impact vegetation, such as cattle grazing and shifting cultivation, often follow topographic features and also play a key role in determining AGB patterns, although these effects may be moderated by accessibility. In this study, we evaluated the potential to predict AGB in a rural landscape, using a set of topographical variables in combination with indicators of accessibility. We modeled linear and non-linear relationships between AGB, topographic variables within the territorial boundaries of six rural communities, and distance to roads. Linear models showed that elevation, slope, topographic wetness index, and tangential curvature could explain up to 21% of AGB. Non-linear models found threshold values for the relationship between AGB and diffuse insolation, topographic position index at 19 × 19 pixels scale and differentiated between groups of communities, improving AGB predictions to 33%. We also found a continuous and positive effect on AGB with increased distance from roads, but also a piecewise relationship that improves the understanding of intensity of human activities. These findings could enable AGB baselines to be constructed at landscape level using freely available data from topographic maps. Such baselines may be of use in national programs under the international policy Reducing Emissions from Deforestation and Forest Degradation

Logging disturbance shifts net primary productivity and its allocation in Bornean tropical forests.

Tropical forests play a major role in the carbon cycle of the terrestrial biosphere. Recent field studies have provided detailed descriptions of the carbon cycle of mature tropical forests, but logged or secondary forests have received much less attention. Here, we report the first measures of total net primary productivity (NPP) and its allocation along a disturbance gradient from old-growth forests to moderately and heavily logged forests in Malaysian Borneo. We measured the main NPP components (woody, fine root and canopy NPP) in old-growth (n = 6) and logged (n = 5) 1 ha forest plots. Overall, the total NPP did not differ between old-growth and logged forest (13.5 ± 0.5 and 15.7 ± 1.5 Mg C ha-1  year-1 respectively). However, logged forests allocated significantly higher fraction into woody NPP at the expense of the canopy NPP (42% and 48% into woody and canopy NPP, respectively, in old-growth forest vs 66% and 23% in logged forest). When controlling for local stand structure, NPP in logged forest stands was 41% higher, and woody NPP was 150% higher than in old-growth stands with similar basal area, but this was offset by structure effects (higher gap frequency and absence of large trees in logged forest). This pattern was not driven by species turnover: the average woody NPP of all species groups within logged forest (pioneers, nonpioneers, species unique to logged plots and species shared with old-growth plots) was similar. Hence, below a threshold of very heavy disturbance, logged forests can exhibit higher NPP and higher allocation to wood; such shifts in carbon cycling persist for decades after the logging event. Given that the majority of tropical forest biome has experienced some degree of logging, our results demonstrate that logging can cause substantial shifts in carbon production and allocation in tropical forests

Hydrological regime of remote catchments with extreme gradients under accelerated change: the Baker basin in Patagonia

The Baker basin (27 000 km2) is located in one of the most pristine and remote areas of the planet. Its hydrological regime is poised to undergo dramatic changes in the near future due to hydropower development and climate change. The basin contains the second-largest lake in South America, and part of a major icefield. This study documents the natural baseline of the Baker River basin, discusses the main hydrological modes and analyses the potential for sustainable management. Annual precipitation varies several-fold from the eastern Patagonian steppes to the North Patagonian Icefield. The westernmost sub-basins are strongly governed by glacier melt with a peak discharge in the austral summer (January–March). The easternmost sub-basins have a much more seasonal response governed by quicker snowmelt in spring (November–December), while they exhibit low flows typical for semi-arid regions during summer and autumn. Topography, vegetation and wetlands may also influence streamflow. The strong spatio-temporal gradients and variability highlight the need for further monitoring, particularly in the headwaters, especially given the severe changes these basins are expected to undergo. The great diversity of hydrological controls and climate change pose significant challenges for hydrological prediction and management

The masked demos: Associational anonymity and democratic practice

The increased use of anonymous digital platforms raises substantive concerns about accountability in digital spaces. However, contemporary evaluations of anonymity focus too narrowly on its protective function: its ability to protect a diversity of speakers and ideas. Drawing on two examples of anonymous political engagements – Publius’s writing of the Federalist Papers and college students’ use of the social media platform Yik Yak – we develop an account of anonymity’s associational function: the processes by which people generate and negotiate collective identities, discussions, and actions in wider publics. As we argue, anonymity’s associational function can (1) generate conditions under which individuals develop collective interests and identities to foster collective action, and (2) enable novel interactions between these individuals and communities and the larger publics of which they are part. We conclude with a discussion of how attention to associational anonymity can contribute to a more nuanced account of democracy in practice

Requirement of the CXXC Motif of Novel Francisella Infectivity Potentiator Protein B FipB, and FipA in Virulence of F. tularensis subsp. tularensis

The lipoprotein encoded by the Francisella tularensis subsp. tularensis locus FTT1103 is essential for virulence; an FTT1103 deletion mutant is defective in uptake and intracellular survival, and mice survive high dose challenges of greater than 108 bacteria. This protein has two conserved domains; one is found in a class of virulence proteins called macrophage infectivity potentiator (Mip) proteins, and the other in oxidoreductase Disulfide Bond formation protein A (DsbA)-related proteins. We have designated the protein encoded by FTT1103 as FipB for Francisella infectivity potentiator protein B. The locus FTT1102 (fipA), which is upstream of fipB, also has similarity to same conserved Mip domain. Deletion and site-specific mutants of fipA and fipB were constructed in the Schu S4 strain, and characterized with respect to intracellular replication and in vivo virulence. A nonpolar fipA mutant demonstrated reduced survival in host cells, but was only slightly attenuated in vivo. Although FipB protein was present in a fipA mutant, the abundance of the three isoforms of FipB was altered, suggesting that FipA has a role in post-translational modification of FipB. Similar to many DsbA homologues, FipB contains a cysteine-any amino acid-any amino acid-cysteine (CXXC) motif. This motif was found to be important for FipB's role in virulence; a deletion mutant complemented with a gene encoding a FipB protein in which the first cysteine was changed to an alanine residue (AXXC) failed to restore intracellular survival or in vivo virulence. Complementation with a gene that encoded a CXXA containing FipB protein was significantly defective in intracellular growth; however, only slightly attenuated in vivo

Host-Adaptation of Francisella tularensis Alters the Bacterium's Surface-Carbohydrates to Hinder Effectors of Innate and Adaptive Immunity

The gram-negative bacterium Francisella tularensis survives in arthropods, fresh water amoeba, and mammals with both intracellular and extracellular phases and could reasonably be expected to express distinct phenotypes in these environments. The presence of a capsule on this bacterium has been controversial with some groups finding such a structure while other groups report that no capsule could be identified. Previously we reported in vitro culture conditions for this bacterium which, in contrast to typical methods, yielded a bacterial phenotype that mimics that of the bacterium's mammalian, extracellular phase.SDS-PAGE and carbohydrate analysis of differentially-cultivated F. tularensis LVS revealed that bacteria displaying the host-adapted phenotype produce both longer polymers of LPS O-antigen (OAg) and additional HMW carbohydrates/glycoproteins that are reduced/absent in non-host-adapted bacteria. Analysis of wildtype and OAg-mutant bacteria indicated that the induced changes in surface carbohydrates involved both OAg and non-OAg species. To assess the impact of these HMW carbohydrates on the access of outer membrane constituents to antibody we used differentially-cultivated bacteria in vitro to immunoprecipitate antibodies directed against outer membrane moieties. We observed that the surface-carbohydrates induced during host-adaptation shield many outer membrane antigens from binding by antibody. Similar assays with normal mouse serum indicate that the induced HMW carbohydrates also impede complement deposition. Using an in vitro macrophage infection assay, we find that the bacterial HMW carbohydrate impedes TLR2-dependent, pro-inflammatory cytokine production by macrophages. Lastly we show that upon host-adaptation, the human-virulent strain, F. tularensis SchuS4 also induces capsule production with the effect of reducing macrophage-activation and accelerating tularemia pathogenesis in mice.F. tularensis undergoes host-adaptation which includes production of multiple capsular materials. These capsules impede recognition of bacterial outer membrane constituents by antibody, complement, and Toll-Like Receptor 2. These changes in the host-pathogen interface have profound implications for pathogenesis and vaccine development

Interrelationship between Dendritic Cell Trafficking and Francisella tularensis Dissemination following Airway Infection

Francisella tularensis, the etiological agent of the inhalation tularemia, multiplies in a variety of cultured mammalian cells. Nevertheless, evidence for its in vivo intracellular residence is less conclusive. Dendritic cells (DC) that are adapted for engulfing bacteria and migration towards lymphatic organs could serve as potential targets for bacterial residence and trafficking. Here, we focus on the in vivo interactions of F. tularensis with DC following airway infection of mice. Lethal airway infection of mice with the live vaccine strain (LVS) results in trafficking of a CD11bhigh/CD11cmed/autofluorescencelow DC subset from the respiratory tract to the draining mediastinal lymph node (MdLN). Simultaneously, a rapid, massive bacterial colonization of the MdLN occurs, characterized by large bacterial foci formation. Analysis of bacteria in the MdLN revealed a major population of extracellular bacteria, which co-exists with a substantial fraction of intracellular bacteria. The intracellular bacteria are viable and reside in cells sorted for DC marker expression. Moreover, in vivo vital staining experiments indicate that most of these intracellular bacteria (∼75%) reside in cells that have migrated from the airways to the MdLN after infection. The correlation between DC and bacteria accumulation in the MdLN was further demonstrated by manipulating DC migration to the MdLN through two independent pathways. Impairment of DC migration to the MdLN, either by a sphingosine-1-phosphate receptor agonist (FTY720) or by the D prostanoid receptor 1 agonist (BW245C), resulted in reduced bacterial colonization of MdLN. Moreover, BW245C treatment delayed the onset of morbidity and the time to death of the infected mice. Taken together, these results suggest that DC can serve as an inhabitation niche for F. tularensis in the early stages of infection, and that DC trafficking plays a role in pathogen dissemination. This underscores the therapeutic potential of DC migration impairing drugs in tularemia treatment

Tipificación de los incendios forestales de Cataluña. Elaboración del mapa de incendios de diseño como herramienta para la gestión forestal

El fuego forestal es hoy en día la perturbación dominante de la mayor parte de los paisajes mediterráneos. La gestión forestal en el siglo XXI y actual contexto de cambio global debe considerar más que nunca los incendios forestales. Para orientar la gestión forestal y desarrollar modelos de gestión que integren el fuego como condicionante es imprescindible un análisis específico de su recurrencia, intensidad y patrón espacial de afectación. Se presenta el proceso para determinar las tipologías de incendios forestales de Cataluña y su relación con la determinación de zonas más vulnerables al fuego. Se han reconstruido y estudiado más de 2.000 incendios, desde el 1968 a la actualidad. Se han descrito para cada incendio, parámetros como la meteorología (situación sinóptica), el patrón de comportamiento (factor dominante, dirección principal, incendio de diseño) y el tiempo desde el último incendio o la estacionalidad. Esta información ha permitido cartografiar el régimen de incendios caracterizando para cada zona, la recurrencia entre fuegos y los incendios de diseño. La caracterización del régimen de incendios permite sentar las bases para la planificación de la gestión forestal, tanto para aspectos relacionados con la rotación y la persistencia a nivel de finca, como para aspectos relacionados con la prevención y extinción de incendios a nivel de paisaje