A study of the action, and the mechanism of action of serotonin on pyramidal neurones in the hippocampal slice preparation

1) Transverse hippocampal slices were prepared from rats and maintained in a recording chamber. Intracellular recording techniques were used to measure the amplitudes of the resting potentials (mean -63.4mV), the action potentials (mean 72.5mV), and the input resistance (mean 16.5M?) of neurones in the CA1 region of the hippocampus. Impalements of neurones could be maintained for up to 9.5hrs. 2) Impaled neurones in the CA1 region of the hippocampus were identified as pyramidal neurones after injection of the fluorescent dye Lucifer Yellow CH and subsequent microscopical examination of the slices. The projections of the apical and basal dendrites were complete and in some cases the axon could be identified and traced toward the subiculum. Injections of pyramidal neurones in the CA3 region and of granule cells in the area dentata demonstrated the different morphologies of the three types of neurone. 3) CA1 neurones were hyperpolarised by up to 10mV by serotonin applied locally by iontophoresis. This response was slow on onset and lasted between 30s and 3min. In contrast, glutamic acid and acetylcholine depolarised these neurones. 4) The amplitude of the hyperpolarisation induced by serotonin was dependent on the amplitude and duration of the iontophoresis current. A decreased input resistance of the neurones was associated with and followed the same time course as the potential change induced by serotonin. 5) The serotonin response was reversed at membrane potentials more negative than the reversal potential which was between -81mV and -104mV. This high reversal potential indicated that the response might be mediated by efflux of potassium ions from the neurones. 6) In ion substitution experiments, the serotonin response was shown to be dependent on the extracellular potassium ion concentration, but was independent of the chloride ion concentration. 7) The serotonin response was blocked by the putative serotonergic antagonist methysergide (100µM). However, the responses of the neurones to serotonin were not affected by cyproheptadine, methergoline, mianserin, and 1-propranolol which have also been proposed to be antagonists of serotonin in the central nervous system. 8) The significance of these results is discussed with reference to previous investigations by other workers

Early Ordovician Seamounts Preserved in the Canadian Cordillera: Implications for the Rift History of Western Laurentia

The breakup of the supercontinent Rodinia and development of the western Laurentian rifted margin are in part recorded by Neoproterozoic to mid-Paleozoic igneous and sedimentary rock successions in the Canadian Cordillera. New bedrock mapping and volcanic facies analysis of Early Ordovician mafic rocks assigned to the Menzie Creek Formation in central Yukon allow reconstruction of the depositional environment during the volcanic eruptions, whole-rock geochemical data constrain the melting depth and crust-mantle source regions of the igneous rocks within the study area, and zircon U-Pb age studies provide determination of the precise timing of submarine eruptions. Menzie Creek Formation volcanic rocks are interlayered with continental slope strata and show lithofacies consistent with those of modern seamount systems. Representative seamount facies contain several kilometers of hyaloclastite breccia and pillow basalt with rare sedimentary rocks. Menzie Creek Formation seamounts form a linear array parallel to the Twopete fault, an ancient extensional or strike-slip fault that localized magmatism along the nascent western Laurentian margin. Zircon grains from two volcanic successions yielded high-precision chemical abrasion–thermal ionization mass spectrometry (CA-TIMS) dates of ca. 484 Ma (Tremadocian), which are interpreted as the age of eruption. Menzie Creek Formation rocks are alkali basalt and have oceanic-island basalt–like geochemical compositions. The whole-rock trace element and Nd-Hf isotope compositions are consistent with the partial melting of subcontinental lithospheric mantle at ~75–100 km depth. Post-rift, Early Ordovician seamounts in central Yukon record punctuated eruptive activity along a rift-related fault, the separation of a continental fragment from western Laurentia, or the oblique post-breakup kinematics from the counterclockwise rotation of Laurentia that facilitated local extension in the passive margin

Radiation belt slot-region filling events: sustained energetic precipitation into the mesosphere

Precipitation of energetic electrons to the atmosphere is both a loss mechanism for radiation belt particles and a means by which the geospace environment influences the Earth's atmosphere; thus it is important to fully understand the extent of this precipitation. A set of polar orbiting satellites have been used to identify periods when energetic charged particles fill the slot region between the inner and outer radiation belts. These suggest that electrons with energies >30 keV penetrate this region, even under levels of modest geomagnetic activity. Those events with sufficient fluxes of particles produce enough ionization to be detected by a ground‐based radar in Antarctica; this precipitation lasts for ~10 days on average. Analysis of these data reveal that the average precipitation penetrates to the stratopause (~55 km altitude). For some (if not all) of these events, the likely cause of the most energetic precipitation is an interaction between (relativistic) electrons and plasmaspheric hiss leading to little, if no, local time variation in precipitation. This does not preclude a longitudinal effect given that all radar measurements are fixed in longitude. During winter months the radar is under the stable southern polar atmospheric vortex. This transports atmospheric species to lower altitudes including the ozone destroying chemicals that are produced by energetic precipitation. Thus the precipitation from the slot region in the southern hemisphere will likely contribute to the destruction of ozone and changes to atmospheric heat balance and chemistry; more work is required to determine the true impact of these events

Investigating energetic electron precipitation through combining ground-based and balloon observations

A detailed comparison is undertaken of the energetic electron spectra and fluxes of two precipitation events that were observed in 18/19 January 2013. A novel but powerful technique of combining simultaneous ground-based subionospheric radio wave data and riometer absorption measurements with X-ray fluxes from a Balloon Array for Relativistic Radiation-belt Electron Losses (BARREL) balloon is used for the first time as an example of the analysis procedure. The two precipitation events are observed by all three instruments, and the relative timing is used to provide information/insight into the spatial extent and evolution of the precipitation regions. The two regions were found to be moving westward with drift periods of 5–11 h and with longitudinal dimensions of ~20° and ~70° (1.5–3.5 h of magnetic local time). The electron precipitation spectra during the events can be best represented by a peaked energy spectrum, with the peak in flux occurring at ~1–1.2 MeV. This suggests that the radiation belt loss mechanism occurring is an energy-selective process, rather than one that precipitates the ambient trapped population. The motion, size, and energy spectra of the patches are consistent with electromagnetic ion cyclotron-induced electron precipitation driven by injected 10–100 keV protons. Radio wave modeling calculations applying the balloon-based fluxes were used for the first time and successfully reproduced the ground-based subionospheric radio wave and riometer observations, thus finding strong agreement between the observations and the BARREL measurements

A synthesis of atmospheric mercury depletion event chemistry in the atmosphere and snow

It was discovered in 1995 that, during the spring time, unexpectedly low concentrations of gaseous elemental mercury (GEM) occurred in the Arctic air. This was surprising for a pollutant known to have a long residence time in the atmosphere; however conditions appeared to exist in the Arctic that promoted this depletion of mercury (Hg). This phenomenon is termed atmospheric mercury depletion events (AMDEs) and its discovery has revolutionized our understanding of the cycling of Hg in Polar Regions while stimulating a significant amount of research to understand its impact to this fragile ecosystem. Shortly after the discovery was made in Canada, AMDEs were confirmed to occur throughout the Arctic, sub-Artic and Antarctic coasts. It is now known that, through a series of photochemically initiated reactions involving halogens, GEM is converted to a more reactive species and is subsequently associated to particles in the air and/or deposited to the polar environment. AMDEs are a means by which Hg is transferred from the atmosphere to the environment that was previously unknown. In this article we review Hg research taken place in Polar Regions pertaining to AMDEs, the methods used to collect Hg in different environmental media, research results of the current understanding of AMDEs from field, laboratory and modeling work, how Hg cycles around the environment after AMDEs, gaps in our current knowledge and the future impacts that AMDEs may have on polar environments. The research presented has shown that while considerable improvements in methodology to measure Hg have been made but the main limitation remains knowing the speciation of Hg in the various media. The processes that drive AMDEs and how they occur are discussed. As well, the role that the snow pack and the sea ice play in the cycling of Hg is presented. It has been found that deposition of Hg from AMDEs occurs at marine coasts and not far inland and that a fraction of the deposited Hg does not remain in the same form in the snow. Kinetic studies undertaken have demonstrated that bromine is the major oxidant depleting Hg in the atmosphere. Modeling results demonstrate that there is a significant deposition of Hg to Polar Regions as a result of AMDEs. Models have also shown that Hg is readily transported to the Arctic from source regions, at times during springtime when this environment is actively transforming Hg from the atmosphere to the snow and ice surfaces. The presence of significant amounts of methyl Hg in snow in the Arctic surrounding AMDEs is important because this species is the link between the environment and impacts to wildlife and humans. Further, much work on methylation and demethylation processes has occurred but these processes are not yet fully understood. Recent changes in the climate and sea ice cover in Polar Regions are likely to have strong effects on the cycling of Hg in this environment; however more research is needed to understand Hg processes in order to formulate meaningful predictions of these changes

Characterization of the Phytochelatin Synthase of Schistosoma mansoni

Treatment for schistosomiasis, which is responsible for more than 280,000 deaths annually, depends exclusively on the use of praziquantel. Millions of people are treated annually with praziquantel and drug resistant parasites are likely to evolve. In order to identify novel drug targets the Schistosoma mansoni sequence databases were queried for proteins involved in glutathione metabolism. One potential target identified was phytochelatin synthase (PCS). Phytochelatins are oligopeptides synthesized enzymatically from glutathione by PCS that sequester toxic heavy metals in many organisms. However, humans do not have a PCS gene and do not synthesize phytochelatins. In this study we have characterized the PCS of S. mansoni (SmPCS). The conserved catalytic triad of cysteine-histidine-aspartate found in PCS proteins and cysteine proteases is also found in SmPCS, as are several cysteine residues thought to be involved in heavy metal binding and enzyme activation. The SmPCS open reading frame is considerably extended at both the N- and C-termini compared to PCS from other organisms. Multiple PCS transcripts are produced from the single encoded gene by alternative splicing, resulting in both mitochondrial and cytoplasmic protein variants. Expression of SmPCS in yeast increased cadmium tolerance from less than 50 µM to more than 1,000 µM. We confirmed the function of SmPCS by identifying PCs in yeast cell extracts using HPLC-mass spectrometry. SmPCS was found to be expressed in all mammalian stages of worm development investigated. Increases in SmPCS expression were seen in ex vivo worms cultured in the presence of iron, copper, cadmium, or zinc. Collectively, these results indicate that SmPCS plays an important role in schistosome response to heavy metals and that PCS is a potential drug target for schistosomiasis treatment. This is the first characterization of a PCS from a parasitic organism

Radar observations of winds, waves and tides in the mesosphere and lower thermosphere over South Georgia island (54◦S, 36◦W) and comparison to WACCM simulations [in review]

The mesosphere and lower thermosphere (MLT) is a dynamic layer of the earth’s atmosphere. This region marks the interface at which neutral atmosphere dynamics begin to influence the ionosphere and space weather. However, our understanding of this region and our ability to accurately simulate it in global circulation models (GCMs) is limited by a lack of observations, especially in remote locations. To this end, a meteor radar was deployed on the remote mountainous island of South Georgia (54° S, 36° W) in the Southern Ocean from 2016 to 2020. The goal of this study is to use these new measurements to characterise the fundamental dynamics of the MLT above South Georgia including large-scale winds, solar tides, planetary waves (PWs) and mesoscale gravity waves (GWs). We first present an improved method for time-height localisation of radar wind measurements and characterise the large-scale MLT winds. We then explore the amplitudes and phases of the diurnal (24 h), semidiurnal (12 h) and terdiurnal (8 h) solar tides at this latitude. We also explore PW activity and find very large amplitudes up to 30 ms−1 for the quasi-2 day wave in summer and show that the dominant modes of the quasi-5, 10 and 16 day waves are westward W1 and W2. We investigate wind variance due to GWs in the MLT and use a new method to show an east-west tendency of GW variance of up to 20 % during summer and a weaker north-south tendency of 0–5 % during winter. This is contrary to the expected tendency of GW directions in the winter stratosphere below, which is a strong suggestion of secondary GW (2GW) observations in the MLT. Lastly, comparison of radar winds to a climatological Whole Atmosphere Community Climate Model (WACCM) simulation reveals a simulated summertime mesopause and zonal wind shear that occur at altitudes around 10 km lower than observed, and southward winds during winter above 90 km altitude in the model that are not seen in observations. Further, wintertime zonal winds above 85 km altitude are eastward in radar observations but in WACCM they are found to weaken and reverse to westward. Recent studies have linked this discrepancy to the impact of 2GWs on the residual circulation which are not included in WACCM. These measurements therefore provide vital constraints that can guide the development of GCMs as they extend upwards into this important region of the atmosphere

Suppression of Chlorella vulgaris Growth by Cadmium, Lead, and Copper Stress and Its Restoration by Endogenous Brassinolide

Brassinosteroids play a significant role in the amelioration of various abiotic and biotic stresses. In order to elaborate their roles in plants subjected to heavy metals stress, Chlorella vulgaris cultures treated with 10−8 M brassinolide (BL) were exposed to 10−6–10−4 M heavy metals (cadmium, lead and copper) application. Under heavy metals stress, the growth and chemical composition (chlorophyll, monosaccharides, and protein content) have been decreased during the first 48 h of cultivation. The inhibitory effect of heavy metals on C. vulgaris cultures was arranged in the following order: copper > lead > cadmium. C. vulgaris cultures treated with BL in the absence or presence of heavy metals showed no differences in the endogenous level of BL. On the other hand, treatment with heavy metals results in BL level very similar to that of control cell cultures. These results suggest that the activation of brassinosteroids biosynthesis, via an increase of endogenous BL, is not essential for the growth and development of C. vulgaris cells in response to heavy metals stress. Simultaneously, BL enhanced the content of indole-3-acetic acid, zeatin, and abscisic acid in cultures treated with heavy metals. Levels per cell of chlorophylls, protein, and monosaccharides are all increased by BL treatment when compared to nontreated control cells. Application of BL to C. vulgaris cultures reduced the accumulation of heavy metals stress on growth, prevented chlorophyll, monosaccharides, and protein loss, and increased phytochelatins content. The arrested growth of C. vulgaris cells treated with heavy metals was restored by the coapplication of BL. It suggested that BL overcame the inhibitory effect of heavy metals. From these results, it can be concluded that BL plays the positive role in the alleviation of heavy metals stress

Exclusion and reappropriation: Experiences of contemporary enclosure among children in three East Anglian schools

Transformations of the landscapes which children inhabit have significant impacts on their lives; yet, due to the limited economic visibility of children’s relationships with place, they have little stake in those transformations. Their experience, therefore, illustrates in an acute way the experience of contemporary enclosure as a mode of subordination. Following fieldwork in three primary schools in South Cambridgeshire, UK, we offer an ethnographic account of children’s experiences of socio-spatial exclusion. Yet, we suggest that such exclusion is by no means an end-point in children’s relationships with place. Challenging assumptions that children are disconnected from nature, we argue that through play and imaginative exploration of their environments, children find ways to rebuild relationships with places from which they find themselves excluded. This is the author accepted manuscript. The final version is available from SAGE via http://dx.doi.org/10.1177/026377581664194

Bioavailability in soils

The consumption of locally-produced vegetables by humans may be an important exposure pathway for soil contaminants in many urban settings and for agricultural land use. Hence, prediction of metal and metalloid uptake by vegetables from contaminated soils is an important part of the Human Health Risk Assessment procedure. The behaviour of metals (cadmium, chromium, cobalt, copper, mercury, molybdenum, nickel, lead and zinc) and metalloids (arsenic, boron and selenium) in contaminated soils depends to a large extent on the intrinsic charge, valence and speciation of the contaminant ion, and soil properties such as pH, redox status and contents of clay and/or organic matter. However, chemistry and behaviour of the contaminant in soil alone cannot predict soil-to-plant transfer. Root uptake, root selectivity, ion interactions, rhizosphere processes, leaf uptake from the atmosphere, and plant partitioning are important processes that ultimately govern the accumulation ofmetals and metalloids in edible vegetable tissues. Mechanistic models to accurately describe all these processes have not yet been developed, let alone validated under field conditions. Hence, to estimate risks by vegetable consumption, empirical models have been used to correlate concentrations of metals and metalloids in contaminated soils, soil physico-chemical characteristics, and concentrations of elements in vegetable tissues. These models should only be used within the bounds of their calibration, and often need to be re-calibrated or validated using local soil and environmental conditions on a regional or site-specific basis.Mike J. McLaughlin, Erik Smolders, Fien Degryse, and Rene Rietr