A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence

Rabbit antibodies against actin and tubulin were used in an indirect immunofluorescence study of the structure of the mitotic spindle of PtK1 cells after lysis under conditions that preserve anaphase chromosome movement. During early prophase there is no antiactin staining associated with the mitotic centers, but by late prophase, as the spindle is beginning to form, a small ball of actin antigenicity is found beside the nucleus; After nuclear envelope breakdown, the actiactin stains the region around each mitotic center, and becomes organized into fibers that run between the chromosomes and the poles. Colchicine blocks this organization, but does not disrupt the staining at the poles. At metaphase the antiactin reveals a halo of ill-defined radius around each spindle pole and fibers that run from the poles to the metaphase plate. Antitubulin shows astral rays, fibers running from chromosomes to poles, and some fibers that run across the metaphase plate. At anaphase, there is a shortening of the antiactin-stained fibers, leaving a zone which is essentially free of actin-staining fluorescence between the separating chromosomes. Antitubulin stains the region between chromosomes and poles, but also reveals substantial fibers running through the zone between separating chromosomes. Cells fixed during cytokinesis show actin in the region of the cleavage furrow, while antitubulin reveals the fibrous spindle remnant that runs between daughter cells. These results suggest that actin is a component of the mammalian mitotic spindle, that the distribution of actin differs from that of tubulin and that the distributions of these two fibrous proteins change in different ways during anaphase

Studies of the marine crustal magnetization at intermediate wavelengths

The data can be filtered at intermediate wavelengths to provde a data set which complements the satellite fields of MAGSAT, TSS and GRM. The filtered marine data set provides a high resolution data set which is closer to the source bodies than satellite survey data. However, the GRM and TSS could provide the necessary resolution to match the filtered sea surface field. The added resolution determines the nature of crustal magnetizations which give rise to the intermediate wavelength field. It is found that remanent magnetization is an important component over the oceans. Crustal deformation and plate motions result in magnetization vectors which differ significantly from the present day field directions. Induced magnetization or GRM are important components over the oceanic plateaus and spreading centers

Extension in the western Ross Sea region-links between Adare Basin and Victoria Land Basin

Spreading in the Adare Basin off north-western Ross Sea (43–26 Ma) and extension in the Victoria Land Basin (VLB, > 36 Ma) are used to constrain the pole of rotation for the Adare Basin, providing a rifting model for the region for the past 45 Ma. The offset from Northern Basin to VLB at about 74°S coincides with the linear Polar-3 magnetic anomaly, inferred to be caused by a major 48 - 34 Ma igneous intrusion. The style of extension apparently changed at about 34 Ma, with the end of intrusion at the Polar-3 anomaly, a change from highly asymmetric extension in Adare Basin, and the onset of major subsidence on the flanks of VLB. Ductile lower crustal and lithospheric flow is proposed as the cause of the inferred thick crust underlying southern Adare Basin, and a result of the constraining of extension to the adjacent contiguous Northern Basin

Pre-eclampsia rates in the United States, 1980-2010: age-period-cohort analysis

Objective: To estimate the contributions of biological aging, historical trends, and birth cohort effects on trends in pre-eclampsia in the United States. Design: Population based retrospective study. Setting: National hospital discharge survey datasets, 1980-2010, United States. Participants: 120 million women admitted to hospital for delivery. Main outcome measures: Temporal changes in rates of mild and severe pre-eclampsia in relation to maternal age, year of delivery, and birth cohorts. Poisson regression as well as multilevel age-period-cohort models with adjustment for obesity and smoking were incorporated. Results: The rate of pre-eclampsia was 3.4%. The age-period-cohort analysis showed a strong age effect, with women at the extremes of maternal age having the greatest risk of pre-eclampsia. In comparison with women delivering in 1980, those delivering in 2003 were at 6.7-fold (95% confidence interval 5.6-fold to 8.0-fold) increased risk of severe pre-eclampsia. Period effects declined after 2003. Trends for severe pre-eclampsia also showed a modest birth cohort effect, with women born in the 1970s at increased risk. Compared with women born in 1955, the risk ratio for women born in 1970 was 1.2 (95% confidence interval 1.1 to 1.3). Similar patterns were also evident for mild pre-eclampsia, although attenuated. Changes in the population prevalence of obesity and smoking were associated with period and cohort trends in pre-eclampsia but did not explain the trends. Conclusions Rates of severe pre-eclampsia have been increasing in the United States and age-period-cohort effects all contribute to these trends. Although smoking and obesity have driven these trends, changes in the diagnostic criteria may have also contributed to the age-period-cohort effects. Health consequences of rising obesity rates in the United States underscore that efforts to reduce obesity may be beneficial to maternal and perinatal health

New Constraints on Plate Tectonic Puzzle of the SW Pacific

A long-standing problem in the tectonics of the southwest Pacific has been the lack of closure of the plate circuit linking the Antarctic, Australia, Lord Howe Rise, and Pacific plates in late Cretaceous and early Tertiary time [Molnar et al., 1975]. Avoiding unacceptable overlaps and underlaps in reconstructions of these plates requires invoking relative motion on one or more nebulous plate boundaries somewhere along the plate circuit, such as between East and West Antarctica, within West Antarctica [Stock and Molnar, 1987], or perhaps between the Lord Howe Rise and Challenger Plateau in the Tasman Sea [Lawver and Gahagan, 1994]. This problem is of more than mere local interest since the motion of the Pacific plate relative to the rest of the globe is constrained through its connection with West Antarctica

Revised Eocene-Oligocene kinematics for the West Antarctic rift system

Past plate motion between East and West Antarctica along the West Antarctic rift system had important regional and global implications. Although extensively studied, the kinematics of the rift during Eocene-Oligocene time still remains elusive. Based on a recent detailed aeromagnetic survey from the Adare and Northern Basins, located in the northwestern Ross Sea, we present the first well-constrained kinematic model with four rotations for Anomalies 12o, 13o, 16y, and 18o (26.5–40.13 Ma). These rotation poles form a cluster suggesting a stable sense of motion during that period of time. The poles are located close to the central part of the rift implying that the local motion varied from extension in the western Ross Sea sector (Adare Basin, Northern Basin, and Victoria Land Basin) to dextral transcurrent motion in the Ross Ice Shelf and to oblique convergence in the eastern end of the rift zone. The results confirm previous estimates of 95 km of extension in the Victoria Land Basin

Morphology and tectonics of the Mid-Atlantic Ridge, 7°–12°S

We present swath bathymetric, gravity, and magnetic data from the Mid-Atlantic Ridge between the Ascension and the Bode Verde fracture zones, where significant ridge–hot spot interaction has been inferred. The ridge axis in this region may be divided into four segments. The central two segments exhibit rifted axial highs, while the northernmost and southernmost segments have deep rift valleys typical of slow-spreading mid-ocean ridges. Bathymetric and magnetic data indicate that both central segments have experienced ridge jumps since ~1 Ma. Mantle Bouguer anomalies (MBAs) derived from shipboard free air gravity and swath bathymetric data show deep subcircular lows centered on the new ridge axes, suggesting that mantle flow has been established beneath the new spreading centers for at least ~1 Myr. Inversion of gravity data indicates that crustal thicknesses vary by ~4 km along axis, with the thickest crust occurring beneath a large axial volcanic edifice. Once the effects of lithospheric aging have been removed, a model in which gravity variations are attributed entirely to crustal thickness variations is more consistent with data from an axis-parallel seismic line than a model that includes additional along-axis variations in mantle temperature. Both geophysical and geochemical data from the region may be explained by the melting of small (<200 km) mantle chemical heterogeneities rather than elevated temperatures. Therefore, there may be no Ascension/Circe plume

A framework for studying behavioral evolution by reconstructing ancestral repertoires

Although extensive behavioral changes often exist between closely related animal species, our understanding of the genetic basis underlying the evolution of behavior has remained limited. Here, we propose a new framework to study behavioral evolution by computational estimation of ancestral behavioral repertoires. We measured the behaviors of individuals from six species of fruit flies using unsupervised techniques and identified suites of stereotyped movements exhibited by each species. We then fit a Generalized Linear Mixed Model to estimate the suites of behaviors exhibited by ancestral species, as well as the intra- and inter-species behavioral covariances. We found that much of intraspecific behavioral variation is explained by differences between individuals in the status of their behavioral hidden states, what might be called their "mood." Lastly, we propose a method to identify groups of behaviors that appear to have evolved together, illustrating how sets of behaviors, rather than individual behaviors, likely evolved. Our approach provides a new framework for identifying co-evolving behaviors and may provide new opportunities to study the genetic basis of behavioral evolution