Mitochondrial Uncoupling Proteins In Mammals And Plants

Uncoupling proteins (UCPs) belong to a distinct cluster of the mitochondrial anion carrier family. Up to five different uncoupling protein types were found in mitochondria of mammals and plants, and recently in fishes, fungi and protozoa. They exhibit a significantly conserved structure with several motifs specific to either the whole cluster or protein type. Uncoupling proteins, as well as the whole mitochondrial anion carrier gene family, probably emerged in evolution before the separation of animal, fungi, and plant kingdoms and originate from an anion/nucleotide or anion/anion transporter ancestor. Mammalian UCP1, UCP2, UCP3, and plant uncoupling proteins pUCP1 and pUCP2 are similar and seem to form one subgroup, whereas UCP4 and BMCP1 belong to a different group. Molecular, biochemical, and phylogenic data suggest that UCP2 could be considered as an UCP-prototype. UCP1 plays its biological role mainly in the non-shivering thermogenesis while the role of the other types is unknown. However, hypotheses have suggested that they are involved in the general balance of basic energy expenditure, protection from reactive oxygen species, and, in plants, in fruit ripening and seed ontogeny.212201212Kuan, J., Saier M.H., Jr., (1993) Res. Microbiol., 144, pp. 671-672Kuan, J., Saier M.H., Jr., (1993) Crit. Rev. Biochem. Mol. Biol., 28, pp. 209-233El Moualij, B., Duyckaerts, C., Lamotte-Brasseur, J., Sluse, F.E., (1997) Yeast, 13, pp. 573-581Palmieri, F., Indiveri, C., Bisaccia, F., Krämer, R., (1993) J. Bioenerg. Biomembr., 25, pp. 525-535Ježek, P., Garlid, K.D., (1990) J. Biol. Chem., 265, pp. 19303-19311Ricquier, D., Kader, J.-C., (1976) Biochem. Biophys. Res. Commun., 73, pp. 577-583Nedergaard, J., Cannon, B., (1992) New Comprehensive Biochemistry: Molecular Mechanisms in Bioenergetics, 23, pp. 385-420. , Ernster, L., ed., Elsevier Science Publishers, AmsterdamKlingenberg, M., (1990) TIBS, 15, pp. 108-112Garlid, K.D., Orosz, D.E., Modrianský, M., Vassanelli, S., Ježek, P., (1996) J. Biol. Chem., 271, pp. 2615-2620Skulachev, V.P., (1991) FEBS Lett., 294, pp. 158-162Fleury, C., (1997) Nature Genetics, 15, pp. 269-272Boss, O., (1997) FEBS Lett., 408, pp. 39-42Mao, W., (1999) FEBS Lett., 443, pp. 326-330Sanchis, D., (1998) J. Biol. Chem., 273, pp. 34611-34615Jaburek, M., (1999) J. Biol. Chem., 274, pp. 26003-26007Ježek, P., Garlid, K.D., (1998) Int. J. Biochem. Cell Biol., 30, pp. 1163-1168Négre-Salvayre, A., (1997) FASEB J., 11, pp. 809-815Solanes, G., Vidal-Puig, A., Grujic, D., Flier, J.S., Lowell, B.B., (1997) J. Biol. Chem., 272, pp. 25433-25436Gong, D.W., He, Y., Karas, M., Reitman, M., (1997) J. Biol. Chem., 272, pp. 24129-24132Zhou, I.-T., (1997) Proc. Nat. Acad. Sci. USA, 94, pp. 6386-6390Vercesi, A.E., Martins, I.S., Silva, M.A.P., Leite, H.M.F., Cuccovia, I.M., Chaimovich, H., (1995) Nature, 375, p. 24Laloi, M., (1997) Nature, 389, pp. 135-136Maia, I.G., Benedetti, C.E., Leite, A., Turcinelli, S.R., Vercesi, A.E., Arruda, P., (1998) FEBS Lett., 429 (3), pp. 403-406Laloi, M., (1997), Accession AJ001264, GeneBankWatanabe, A., Nakazono, M., Tsutsumi, N., Hirai, A., (1999) Plant Cell Physiol., 40, pp. 1160-1166Ito, K., (1999) Plant. Sci., 149, pp. 167-173Borecký, J., (2000) J. Biol. Chem., , unpublished observationJežek, P., Garlid, K.D., (1998) Int. J. Biochem. Cell Biol., 30, pp. 1163-1168Jaburek, M., (1999) J. Biol. Chem., 274, pp. 26003-26007Ježek, P., Costa, A.D.T., Vercesi, A.E., (1996) J. Biol. Chem., 271, pp. 32743-32748Ježek, P., Costa, A.D.T., Vercesi, A.E., (1997) J. Biol. Chem., 272, pp. 24272-24278Costa, A.D.T., Nantes, I.L., Ježek, P., Leite, A., Arruda, P., Vercesi, A.E., (1999) J. Bioenerg. Biomembr., 31, pp. 527-533Vercesi, A.E., Chaimovich, H., Cuccovia, I.M., (1997) Recent Res. Devel. Plant Physiol., 1, pp. 85-91Ježek, P., (1998) Biochim. Biophys. Acta, 1365, pp. 319-327Vercesi, A.E., Ježek, P., Costa, A.D.T., Kowaltowski, A.J., Maia, I.G., Arruda, P., (1998) Plant Mitochondria: From Gene to Function, pp. 435-440. , (Moller, I. M., Gardeström, P., Glimelius, K., and Glaser, E., eds.), Backhuys Publishers, Leiden, The NetherlandsKowaltowski, A.J., Costa, A.D.T., Vercesi, A.E., (1998) FEBS Lett., 425, pp. 213-216Nantes, I.L., Fagian, M.M., Catisti, R., Arruda, P., Maia, I.G., Vercesi, A.E., (1999) FEBS Lett., 457, pp. 103-106Sluse, F.E., Almeida, A.M., Jarmuszkiewicz, W., Vercesi, A.E., (1998) FEBS Lett., 433, pp. 237-240Jarmuszkiewicz, W., Almeida, A.M., Sluse-Goffart, C., Sluse, F.E., Vercesi, A.E., (1998) J. Biol. Chem., 273, pp. 34882-34886Almeida, A.M., Jarmuszkiewicz, W., Khomsi, H., Arruda, P., Vercesi, A.E., Sluse, F.E., (1999) Plant Physiol., 119, pp. 1324-1329Sluse, F.E., Almeida, A.M., Jarmuszkiewicz, W., Vercesi, A.E., (1998) FEBS Lett., 433, pp. 237-240Nicholls, D.G., Locke, R.M., (1984) Physiol. Rev., 64, pp. 1-64Cannon, B., Nedergaard, J., (1985) Essays Biochem., 20, pp. 110-164Himms-Hagen, J., (1990) FASEB J., 4, pp. 2890-2898Klaus, S., Casteilla, L., Bouillaud, F., Ricquier, D., (1991) Int. J. Biochem., 23, pp. 791-801Ricquier, D., Bouillaud, F., (1997) Prog. Nucleic Acid Res. Mol. Biol., 56, pp. 83-108Ricquier, D., Casteilla, L., Bouillaud, F., (1991) FASEB J., 5, pp. 2237-2242Silva, J.E., Rabelo, R., (1997) Eur. J. Endocrinol., 136, pp. 251-264Himms-Hagen, J., Ricquier, D., (1998) Handbook of Obesity, pp. 415-441. , (Bray, G., Bouchard, C., and James, W., eds), Marcel Dekker, New York, Basel, Hong KongBoss, O., Muzzin, P., Giacobino, J.P., (1998) Eur. J. Endocrinol., 139, pp. 1-9Rial, E., Gonzalez-Barroso, M.M., Fleury, C., Bouillaud, F., (1998) Biofactors, 8, pp. 209-219Freake, H.C., (1998) Nutr. Rev., 56, pp. 185-189Klingenberg, M., Huang, S.G., (1999) Biochim. Biophys. Acta, 1415, pp. 271-296Schrauwen, P., Walder, K., Ravussin, E., (1999) Obes. Res., 7, pp. 97-105Ježek, P., Garlid, K.D., (1998) Int. J. Biochem. Cell Biol., 30, pp. 1163-1168Ricquier, D., (1999) J. Intern. Med., 245, pp. 637-642Bailey, T.L., Elkan, C., (1994) Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology, pp. 28-36. , AAAI Press, Menlo Park, CaliforniaBailey, T.L., Gribskov, M., (1998) Bioinformatics, 14, pp. 48-54Cassard, A.M., (1990) J. Cell. Biochem., 43, pp. 255-264Kozak, L.P., Britton, J.H., Kozak, U.C., Wells, J.M., (1988) J. Biol. Chem., 263, pp. 12274-12277Hilbert, P., (1991) Nature, 353, pp. 521-529Otsen, M., Den Bieman, M., Van Zutphen, L.F., (1993) J. Hered., 84, pp. 149-151Szpirer, C., (1998) Mamm. Genome, 9, pp. 721-734Pecqueur, C., (1999) Biochem. Biophys. Res. Commun., 255, pp. 40-46Gong, D.W., He, Y., Reitman, M.L., (1999) Biochem. Biophys. Res. Commun., 256, pp. 27-32Surwit, R.S., (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 4061-4065Fleury, C., (1997) Nat. Genet., 15, pp. 269-272Kaisaki, P.J., Woon, P.Y., Wallis, R.H., Monaco, A.P., Lathrop, M., Gauguier, D., (1998) Mamm. Genome, 9, pp. 910-912Gimeno, R.E., (1997) Diabetes, 46, pp. 900-906Ricquier, D., Bouillaud, F., (2000) Biochem. J., 345, pp. 161-179Solanes, G., Vidal-Puig, A., Grujic, D., Flier, J.S., Lowell, B.B., (1997) J. Biol. Chem., 272, pp. 25433-25436Laloi, M., Klein, M., Sanchis, D., Fleury, C., (1999) Arabidopsis thaliana ucp gene, exons 1-9, , GeneBank record, ACCESSION Y18291Stuart, J.A., Harper, J.A., Brindle, K.M., Brand, M.D., (1999) Biochim. Biophys. Acta, 1413, pp. 50-54Jarmuszkiewicz, W., Sluse-Goffart, C.M., Hryniewiecka, L., Sluse, F.E., (1999) J. Biol. Chem., 274, pp. 23198-23202Jarmuszkiewicz, W., Milani, G., Fortes, F., Schreiber, A.Z., Sluse, F.E., Vercesi, A., (2000) FEBS Lett., 467, pp. 145-149Uemura, S.A., Luo, S., Moreno, S.N.J., Docampo, R., (2000) J. Biol. Chem., 275, pp. 9709-9715Aquila, H., Link, T., Klingenberg, M., (1985) EMBO J., 4, pp. 2369-2376Winkler, E., Klingenberg, M., (1994) J. Biol. Chem., 269, pp. 2508-2515Haupts, U., Tittor, J., Bamberg, E., Oesterheld, D., (1997) Biochemistry, 36, pp. 2-7Rial, E., (1999) EMBO J., 18, pp. 5827-583

The 100 most eminent psychologists of the 20th century.

A rank-ordered list was constructed that reports the first 99 of the 100 most eminent psychologists of the 20th century. Eminence was measured by scores on 3 quantitative variables and 3 qualitative variables. The quantitative variables were journal citation frequency, introductory psychology textbook citation frequency, and survey response frequency. The qualitative variables were National Academy of Sciences membership, election as American Psychological Association (APA) president or receipt of the APA Distinguished Scientific Contributions Award, and surname used as an eponym. The qualitative variables were quantified and combined with the other 3 quantitative variables to produce a composite score that was then used to construct a rank-ordered list of the most eminent psychologists of the 20th century. The discipline of psychology underwent a remarkable transformation during the 20th cen-tury, a transformation that included a shift away from the European-influenced philosophical psychology of the late 19th century to th

Sequence Similarities Of Protein Kinase Substrates And Inhibitors With Immunoglobulins And Model Immunoglobulin Homologue: Cell Adhesion Molecule From The Living Fossil Sponge Geodia Cydonium. Mapping Of Coherent Database Similarities And Implications For Evolution Of Cdr1 And Hypermutation

Sequences of immunoglobulin (Ig) domains of adhesive molecule GSAMS from the living fossil sponge Geodia cydonium were compared with the important motif of peptide protein kinase substrates and inhibitors (PKSI), detail PKSI sequences, and a common template sequence, derived from structures determined previously. We found the site-restricted sequence similarities to these peptide sequences predominantly in the GSAM Ig1 domain of GSAMS in the domain region related to corresponding Ig similarities detected earlier. Additional sequence block-related analysis revealed the presence of CDR1-like segments within PKSI-related regions and resulted in the detection of increased numbers of hypermutation motifs just in the CDR1-like segment of GSAM Ig1 (GSAM(cdr1.1)). In the following database searches with PKSI-related regions and GSAM(cdr1.1) we looked for: (i) peptide similarities present in the context of Ig domains or related structures in a large range of species from Archaea to Vertebrata, and (ii) some special nucleotide similarities. © 2004 Institute of Microbiology, Academy of Sciences of the Czech Republic.49321924

Management and Management Science in 15 Years: A Vision of the Future under the Influence of New Information Technologies

Uncoupling mitochondrial proteins (UCPs) belong to a discrete family within the mitochondrial anion carrier superfamily. Several uncoupling protein types have been found in mitochondria from mammals and plants, as well as in fishes, fungi, and protozoa. Mammalian UCPs and plant uncoupling proteins (PUMPs) form five distinct subfamilies. Only subfamily III contains both plant and animal uncoupling proteins, as well as UCPs from primitive eukaryotic organisms, which suggest that this group may represent an ancestral cluster from which other UCPs/PUMPs may have evolved. Genetic data indicate that UCPs/PUMPs are regulated at the transcriptional, post-transcriptional, and translational levels. Tissue/organ- and stress-specific gene expression suggests that UCPs/PUMPs are involved in the general balance of basic energy expenditure, protection against reactive oxygen species, and thermogenesis. Finally, the simultaneous occurrence of PUMP and alternative oxidase, another energy-dissipating system in plant mitochondria, raises the question of their response to biotic and abiotic stress at the transcriptional and functional levels.254173220922

Prevention of spontaneous autoimmunity to dna in nzb/swiss mice by treatment with natural double-stranded rna.

Data are presented demonstrating the prevention of spontaneous autoimmune disease in NZB/Swiss mice treated natural double-stranded RNA. The successful treatment of animals was followed both by improvement in clinical manifestations and by the migration inhibition test performed with DNA as antigen. The reasons for the successful employment of natural double-stranded RNA are thought to be in its physicochemical properties

Mutational analysis of Arabidopsis thaliana plant uncoupling mitochondrial protein

In this study, point mutations were introduced in plant uncoupling mitochondrial protein AtUCP1, a typical member of the plant uncoupling protein (UCP) gene subfamily, in amino acid residues Lys147, Arg155 and Tyr269, located inside the so-called UCP-signatures, and in two more residues, Cys28 and His83, specific for plant UCPs. The effects of amino acid replacements on AtUCP1 biochemical properties were examined using reconstituted proteoliposomes. Residue Arg155 appears to be crucial for AtUCP1 affinity to linoleic acid (LA) whereas His83 plays an important role in AtUCP1 transport activity. Residues Cys28, Lys147, and also Tyr269 are probably essential for correct protein function, as their substitutions affected either the AtUCP1 affinity to LA and its transport activity, or sensitivity to inhibitors (purine nucleotides). Interestingly, Cys28 substitution reduced ATP inhibitory effect on AtUCP1, while Tyr269Phe mutant exhibited 2.8-fold increase in sensitivity to ATP, in accordance with the reverse mutation Phe267Tyr of mammalian UCP1. (C) 2007 Elsevier B.V. All fights reserved.1767121412141

ZmPUMP encodes a fully functional monocot plant uncoupling mitochondrial protein whose affinity to fatty acid is increased with the introduction of a His pair at the second matrix loop

Uncoupling proteins (UCPs) are specialized mitochondrial transporter proteins that uncouple respiration from ATP synthesis. In this study, cDNA encoding maize uncoupling protein (ZmPUMP) was expressed in Eycherichia coli and recombinant ZmPUMP reconstituted in liposomes. ZmPUMP activity was associated with a linoleic acid (LA)-mediated H+ efflux with K-m of 56.36 +/- 0.27 mu M and V-max of 66.9 mu mol H+ min(-1) (mg prot)(-1). LA-mediated H+ fluxes were sensitive to ATP inhibition with K-i of 2.61 +/- 0.36 mM (at pH 7.2), a value similar to those for dicot UCPs. ZmPUMP was also used to investigate the importance of a histidine pair present in the second matrix loop of mammalian UCP1 and absent in plant UCPs. ZmPUMP with introduced His pair (Lys155His and Ala157His) displayed a 1.55-fold increase in LA-affinity while its activity remained unchanged. Our data indicate conserved properties of plant UCPs and suggest an enhancing but not essential role of the histidine pair in proton transport mechanism. (c) 2006 Elsevier Inc. All rights reserved.344119419

Psychological distress and streamlined BreastScreen follow-up assessment versus standard assessment

Objectives: To establish whether altered protocol characteristics of streamlined StepDown breast assessment clinics heightened or reduced the psychological distress of women in attendance compared with standard assessment. Willingness to attend future screening was also compared between the assessment groups. Design: Observational, prospective study of women attending either a mammogram-only StepDown or a standard breast assessment clinic. Women completed questionnaires on the day of assessment and 1 month later. Participants and setting: Women attending StepDown (136 women) or standard assessment clinics (148 women) at a BreastScreen centre between 10 November 2009 and 7 August 2010. Main outcome measures: Breast cancer worries; positive and negative psychological consequences of assessment (Psychological Consequences Questionnaire); breast cancer-related intrusion and avoidance (Impact of Event Scale); and willingness to attend, and uneasiness about, future screening. Results: At 1-month follow-up, no group differences were evident between those attending standard and StepDown clinics on breast cancer worries (P=0.44), positive (P =0.88) and negative (P =0.65) consequences, intrusion (P=0.64), and avoidance (P =0.87). Willingness to return for future mammograms was high, and did not differ between groups (P =0.16), although higher levels of unease were associated with lessened willingness to rescreen (P=0.04). Conclusions: There was no evidence that attending streamlined StepDown assessments had different outcomes in terms of distress than attending standard assessment clinics for women with a BreastScreen-detected abnormality. However, unease about attending future screening was generally associated with less willingness to do so in both groups; thus, there is a role for psycho-educational intervention to address these concerns.5 page(s