Sarcomas primarios de hueso: estudio por citometría estática mediante análisis digital de imagen

Se presenta un estudio morfométrico y densitométrico mediante análisis digital de imagen de una serie de 50 tumores óseos malignos (32 osteosarcomas, 12 condrosarcomas y 6 histiocitomas fibrosos malignos de hueso), con el fin de evaluar la utilidad de la técnica para establecer el grado y el pronóstico de estas neoplasias. Las variables morfométricas y la disposición de la cromatina guardan una estrecha relación con el grado histológico (Spearman; p < 0,05) y muestran diferencias estadísticamente significativas entre los tumores considerados de alto y bajo grado en base a su presentación y evolución clínica. La ploidía estimada por densitometría óptica se comporta como un factor pronóstico independiente en el análisis multivariante de la serie de tumores de alto grado (COX; p < 0,001). La citometría estática, aunque más lenta e imprecisa que la citometría de flujo en la determinación de los picos de ploidía, permite analizar el modelo de cromatina y contribuye objetivamente al establecimiento del grado biológico de los tumores óseos.A densitometry and morphometric assessment by static image cytometry of 50 malignant bone tumors (32 osteosarcomas, 12 chondrosarcomas, and 6 malignant fibrous histiocytomas of bone) was performed in order to assess the validity of the technic in the pathobiological and prognostic evaluation of these neoplasms. A strong correlation between the morphometric features, cromatine distribution and histologic grade was found (Spearman; p < 0.05). These parameters are also statistically different for hight and low grade tumors in clinical staging. The stimation of DNA ploidy by optic densitometry is a significant prognostic factor in the multivariant analysis for the high grade tumors (COX; p < 0.001). Although Flow cytometry is a better procedure to evaluate DNA ploidy peaks, our study reveals static image cytometry as a useful technic for determine the cromatine model and objective assessment of the biological grade of bone neoplasms

Fox-Fordyce Disease

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Electromiograph Parameters in Thedysfunctional Patient with Open Bite

INTRODUCTION: Open bite is an occlusal problem that appears in 0.5% of the population, and is more frequent in women. There is correlation between open bite and dysfunctional syndrome with TMJ disorders. AIM OF THE STUDY: We attempted to analyze muscular parameters in patients with open bite treated with an occlusal splint by EMG recordings. MATERIAL AND METHODS: Myotronics K6 electromiograph can provide information in the following situations: maximum force bite, lateral and protrusion movements, with and without an occlusal splint. RESULTS: In maximum force bite with the occlusal splint more activity was found in the elevator muscles with a lack of dygastric activity. There was less EMG activity in protrusive and lateral movements with occlusal splint. CONCLUSION: With better support between the dental arches (occlusal splint) more activity was obtained in the elevators when biting, and when interferences were eliminated activity in the contralateral muscles was reduced

New therapeutic options in SAPHO syndrome: anti - TNF - α

El síndrome SAPHO se caracteriza por la presencia de manifestaciones ostoarticulares junto con lesiones cutáneas diversas como acné, psoriasis pustulosa y pustulosis palmoplantar. Se han empleado diversos fármacos para el control de la enfermedad con resultados variados, recientemente se han introducido los anti-TNF-á, con buena respuesta de las manifestaciones cutáneas y osteoarticulares. Se discute la utilidad de estos nuevos fármacos para el tratamiento de esta patología.SAPHO syndrome is characterised by the presence of osteoarticular symptoms along with a variety of cutaneous lesions such as acne, pustular psoriasis and palmoplantar pustulosis. Several agents have been used in order to control this disorder, with different levels of success. Recently, TNF-á blocking agents have been introduced in the treatment of SAPHO syndrome, usually with good response of both skin and osteoarticular manifestations. The usefulness of these new drugs is discussed in the present manuscript

Poly[ADP-Ribose] Polymerase-1 Expression Is Related To Cold Ischemia, Acute Tubular Necrosis, and Delayed Renal Function In Kidney Transplantation

Cold ischemia time especially impacts on outcomes of expanded-criteria donor (ECD) transplantation. Ischemia-reperfusion (IR) injury produces excessive poly[ADP-Ribose] Polymerase-1 (PARP-1) activation. The present study explored the hypothesis that increased tubular expression of PARP-1 contributes to delayed renal function in suboptimal ECD kidney allografts and in non-ECD allografts that develop posttransplant acute tubular necrosis (ATN)