Temperature sensitivity as a microbial trait

2017 Summer.Includes bibliographical references.Reaction rates in biological systems are strongly controlled by temperature, yet the degree to which temperature sensitivity varies for different enzymes and microorganisms is being largely reformulated. The Arrhenius equation is the most commonly used model over the last century that predicts reaction rate response with temperature. However, the Arrhenius equation does not account for large heat capacities associated with enzymes in biological reactions, thus creating significant deviations from predicted reaction rates. A relatively new model, Macromolecular Rate Theory (MMRT), modifies the Arrhenius equation by accounting for the temperature dependence of these large heat capacities found in biological reactions. Using the MMRT model I have developed a novel framework to assess temperature sensitivity as a biological trait through a series of experiments. This work provides evidence that microbes and enzymes can have distinct heat capacities, and thus distinct temperature sensitivities, independent of their external environment. I first assessed temperature sensitivity of soil CO2 production from different soil microbial communities and then worked with pure cultures to examine temperature sensitivity of enzyme activities from soil microbial isolates. From these experiments I determined that temperature sensitivity varies based on genetic variation of the microbe and substrate type as well as examined the importance of using MMRT over the Arrhenius equation. Finally, I used a meta-analysis to analyze the distribution of temperature sensitivity traits to look across a variety of biological systems (e.g., the food industry, wastewater treatment, soils). I found that temperature sensitivity traits vary with organism type, environment, process type, and biodiversity. Exploring temperature sensitivity as a trait allows for new insights of soil microbes from an ecological perspective as well has the potential to inform ecosystem climate models

Measurement of quasi-elastic 12C(p,2p) scattering at high momentum transfer

We measured the high-momentum quasi-elastic 12C(p,2p) reaction (at center of mass angle near 90 degrees) for 6 and 7.5 GeV/c incident protons. The three-momentum components of both final state protons were measured and the missing energy and momentum of the target proton in the nucleus were determined. The validity of the quasi-elastic picture was verified up to Fermi momenta of about 450 MeV/c, where it might be questionable. Transverse and longitudinal Fermi momentum distributions of the target proton were measured and compared to independent particle models which do not reproduce the large momentum tails. We also observed that the transverse Fermi distribution gets wider as the longitudinal component increases in the beam direction, in contrast to a simple Fermi gas model.Comment: 4 pages including 3 figure

Longitudinal Momentum Fraction X_L for Two High P_t Protons in pp->ppX Reaction

We present an analysis of new data from Experiment E850 at BNL. We have characterized the inclusive cross section near the endpoint for pp exclusive scattering in Hydrogen and in Carbon with incident beam energy of 6 GeV. We select events with a pair of back-to-back hadrons at large transverse momentum. These cross sections are parameterized with a form $\frac{d \sigma}{d X_{L}}$ $\sim(1-X_{L})^{p}$, where ${X_{L}}$ is the ratio of the longitudinal momentum of the observed pair to the total incident beam momentum. Small value of $p$ may suggest that the number of partons participating in the reaction is large and reaction has a strong dependence on the center-of-mass energy. We also discuss nuclear effects observed in our kinematic region.Comment: 4 pages, 2 figures, to be published in Proceedings of CIPANP2000, Quebec, May 22-28, 2000, requires aipproc.sty(included

European Dermatology Forum guidelines on topical photodynamic therapy 2019 Part 2: emerging indications – field cancerization, photorejuvenation and inflammatory/infective dermatoses

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Energy Dependence of Nuclear Transparency in C(p,2p) Scattering

The transparency of carbon for (p,2p) quasi-elastic events was measured at beam energies ranging from 6 to 14.5 GeV at 90 degrees c.m. The four momentum transfer squared q*q ranged from 4.8 to 16.9 (GeV/c)**2. We present the observed energy dependence of the ratio of the carbon to hydrogen cross sections. We also apply a model for the nuclear momentum distribution of carbon to normalize this transparency ratio. We find a sharp rise in transparency as the beam energy is increased to 9 GeV and a reduction to approximately the Glauber level at higher energies.Comment: 4 pages, 2figures, submitted to PR

The Molecular Diversity of Freshwater Picoeukaryotes Reveals High Occurrence of Putative Parasitoids in the Plankton

Eukaryotic microorganisms have been undersampled in biodiversity studies in freshwater environments. We present an original 18S rDNA survey of freshwater picoeukaryotes sampled during spring/summer 2005, complementing an earlier study conducted in autumn 2004 in Lake Pavin (France). These studies were designed to detect the small unidentified heterotrophic flagellates (HF, 0.6–5 µm) which are considered the main bacterivores in aquatic systems. Alveolates, Fungi and Stramenopiles represented 65% of the total diversity and differed from the dominant groups known from microscopic studies. Fungi and Telonemia taxa were restricted to the oxic zone which displayed two fold more operational taxonomic units (OTUs) than the oxycline. Temporal forcing also appeared as a driving force in the diversification within targeted organisms. Several sequences were not similar to those in databases and were considered as new or unsampled taxa, some of which may be typical of freshwater environments. Two taxa known from marine systems, the genera Telonema and Amoebophrya, were retrieved for the first time in our freshwater study. The analysis of potential trophic strategies displayed among the targeted HF highlighted the dominance of parasites and saprotrophs, and provided indications that these organisms have probably been wrongfully regarded as bacterivores in previous studies. A theoretical exercise based on a new ‘parasite/saprotroph-dominated HF hypothesis’ demonstrates that the inclusion of parasites and saprotrophs may increase the functional role of the microbial loop as a link for carbon flows in pelagic ecosystems. New interesting perspectives in aquatic microbial ecology are thus opened