Immunocytochemical demonstration of PTHrP protein in neoplastic tissue of HTLV-1 positive human adult T cell leukaemia/lymphoma: implications for the mechanism of hypercalcaemia.

The infiltrated tissues from seven West Indian patients with HTLV-1 positive adult T cell lymphoma/leukaemia (ATLL) have been analysed by immunocytochemical techniques for the presence of immunoreactive parathyroid hormone-related protein (PTHrP), a hormonal mediator of humoral hypercalcaemia of malignancy. Six of the seven were hypercalcaemic at some stage of the course of their disease. Four of the six evaluable patients showed evidence of specific cellular and extracellular expression of PTHrP protein in neoplastic tissues. This finding suggests that PTHrP may be involved in the production of hypercalcaemia in at least some cases of T cell lymphoma - proof of a causal relationship however must await the demonstration of tissue release of PTHrP resulting in raised circulating hormone levels

Superflares on Ordinary Solar-Type Stars

Short duration flares are well known to occur on cool main-sequence stars as well as on many types of `exotic' stars. Ordinary main-sequence stars are usually pictured as being static on time scales of millions or billions of years. Our sun has occasional flares involving up to $\sim 10^{31}$ ergs which produce optical brightenings too small in amplitude to be detected in disk-integrated brightness. However, we identify nine cases of superflares involving $10^{33}$ to $10^{38}$ ergs on normal solar-type stars. That is, these stars are on or near the main-sequence, are of spectral class from F8 to G8, are single (or in very wide binaries), are not rapid rotators, and are not exceedingly young in age. This class of stars includes many those recently discovered to have planets as well as our own Sun, and the consequences for any life on surrounding planets could be profound. For the case of the Sun, historical records suggest that no superflares have occurred in the last two millennia.Comment: 16 pages, accepted for publication in Ap

The evolution of plasticity of dauer larva developmental arrest in the nematode Caenorhabditis elegans.

Organisms can end up in unfavourable conditions and to survive this they have evolved various strategies. Some organisms, including nematodes, survive unfavourable conditions by undergoing developmental arrest. The model nematode Caenorhabditis elegans has a developmental choice between two larval forms, and it chooses to develop into the arrested dauer larva form in unfavourable conditions (specifically, a lack of food and high population density, indicated by the concentration of a pheromone). Wild C. elegans isolates vary extensively in their dauer larva arrest phenotypes, and this prompts the question of what selective pressures maintain such phenotypic diversity? To investigate this we grew C. elegans in four different environments, consisting of different combinations of cues that can induce dauer larva development: two combinations of food concentration (high and low) in the presence or absence of a dauer larva-inducing pheromone. Five generations of artificial selection of dauer larvae resulted in an overall increase in dauer larva formation in most selection regimes. The presence of pheromone in the environment selected for twice the number of dauer larvae, compared with environments not containing pheromone. Further, only a high food concentration environment containing pheromone increased the plasticity of dauer larva formation. These evolutionary responses also affected the timing of the worms' reproduction. Overall, these results give an insight into the environments that can select for different plasticities of C. elegans dauer larva arrest phenotypes, suggesting that different combinations of environmental cues can select for the diversity of phenotypically plastic responses seen in C. elegans.We would like to thank Henrique Teotonio for the gift of the G140.A population, Louise Hughes and Laura Weldon for technical help, two anonymous reviewers for their comments, and NERC for funding.This is the final published version of the article. It was originally published in Ecology and Evolution (Diaz SA, Viney M, Ecology and Evolution 2015, 5(6), 1343–1353, doi:10.1002/ece3.1436) http://dx.doi.org/10.1002/ece3.143

High Velocity Line Emission in the NLR of NGC 4151

Narrow-band imaging of the nuclear region of NGC 4151 with the Hubble Space Telescope is presented. The filter bandpasses isolate line emission in various high velocity ranges in several ions. Slitless and long-slit spectra of the region with the Space Telescope Imaging Spectrograph also indicate the locations of high velocity gas. These emission regions are faint and are interspersed among the bright emission clouds seen in direct images. They have radial velocities up to 1400 km/s relative to the nucleus, and are found in both approach and recession on both sides of the nucleus. This contrasts strongly with the bright emission line clouds which have been discussed previously as showing bidirectional outflow with velocities within 400 km/s of the nucleus. We discuss the possible connections of the high velocity material with the radio jet and the nuclear radiation.Comment: 12 pages plus 6 figures, to be published in A

Flourescent liquid pyrene derivative-in-water mircoemulsions

A fluorescent liquid pyrene derivative with a high fluorescence quantum yield (65%) in the bulk state is reported. With this as the sole oil phase, stable luminescent oil-in-water microemulsions have been prepared. Increasing the loading of liquid pyrene swells the droplets, as detected by small-angle neutron scattering. These larger droplets have a greater proportion of pyrene excimer emission contribution in their photoluminescence spectra, which leads to a red shift in the chromaticity of the emission

STIS Echelle Observations of the Seyfert Galaxy NGC 4151: Physical Conditions in the Ultraviolet Absorbers

We have examined the physical conditions in intrinsic UV-absorbing gas in the Seyfert galaxy NGC 4151, using echelle spectra obtained with the Space Telescope Imaging Spectrograph (STIS). We confirm the presence of the kinematic components detected in earlier GHRS observations as well as a new broad absorption feature at a radial velocity of -1680 km/s. The UV continuum of NGC 4151 decreased by a factor of 4 over the previous two years, and we argue the changes in the column density of the low ionization absorption lines associated with the broad component at -490 km/s reflect the decrease in the ionizing flux. Most of the strong absorption lines (e.g., N V, C IV, Si IV) from this component are saturated, but show substantial residual flux in their cores, indicating that the absorber does not fully cover the source of emission. Our interpretation is that the unocculted light is due to scattering by free electrons from an extended region, which reflects continuum, emission lines, and absorption lines. We have been able to constrain the densities for the kinematic components based on absorption lines from metastable states of C III and Fe II, and/or the ratios of ground and fine structure lines of O I,C II, and Si II. We have generated a set of photoionization models which match the ionic column densities for each component during the present low flux state and those seen in previous high flux states with the GHRS and STIS, confirming that the absorbers are photoionized and respond to the changes in the continuum flux. We have been able to map the relative radial positions of the absorbers, and find that the gas decreases in density with distance. None of the UV absorbers is of sufficiently large column density or high enough ionization state to account for the X-ray absorption.Comment: 46 pages (Latex), 14 figures (postscript), plus a landscape table (Latex), to appear in the Astrophysical Journa

Observations of H3+ in the Diffuse Interstellar Medium

Surprisingly large column densities of H3+ have been detected using infrared absorption spectroscopy in seven diffuse cloud sightlines (Cygnus OB2 12, Cygnus OB2 5, HD 183143, HD 20041, WR 104, WR 118, and WR 121), demonstrating that H3+ is ubiquitous in the diffuse interstellar medium. Using the standard model of diffuse cloud chemistry, our H3+ column densities imply unreasonably long path lengths (~1 kpc) and low densities (~3 cm^-3). Complimentary millimeter-wave, infrared, and visible observations of related species suggest that the chemical model is incorrect and that the number density of H3+ must be increased by one to two orders of magnitude. Possible solutions include a reduced electron fraction, an enhanced rate of H2 ionization, and/or a smaller value of the H3+ dissociative recombination rate constant than implied by laboratory experiments.Comment: To be published in Astrophysical Journal, March 200

Cloud Structure and Physical Conditions in Star-Forming Regions from Optical Observations. II. Analysis

To complement the optical absorption-line survey of diffuse molecular gas in Paper I, we obtained and analyzed far ultraviolet H$_2$ and CO data on lines of sight toward stars in Cep OB2 and Cep OB3. Possible correlations between column densities of different species for individual velocity components, not total columns along a line of sight as in the past, were examined and were interpreted in terms of cloud structure. The analysis reveals that there are two kinds of CH in diffuse molecular gas: CN-like CH and CH$^+$-like CH. Evidence is provided that CO is also associated with CN in diffuse molecular clouds. Different species are distributed according to gas density in the diffuse molecular gas. Both calcium and potassium may be depleted onto grains in high density gas, but with different dependences on local gas density. Gas densities for components where CN was detected were inferred from a chemical model. Analysis of cloud structure indicates that our data are generally consistent with the large-scale structure suggested by maps of CO millimeter-wave emission. On small scales, the gas density is seen to vary by factors greater than 5.0 over scales of $\sim$ 10,000 AU. The relationships between column densities of CO and CH with that of H$_2$ along a line of sight show similar slopes for the gas toward Cep OB2 and OB3, but the CO/H$_2$ and CH/H$_2$ ratios tend to differ which we ascribe to variation in average density along the line of sight.Comment: 49 pages, 9 figures, accepted by Ap

Evidence of a Supermassive Black Hole in the Galaxy NGC 1023 from the Nuclear Stellar Dynamics

We analyze the nuclear stellar dynamics of the SB0 galaxy NGC 1023, utilizing observational data both from the Space Telescope Imaging Spectrograph aboard the Hubble Space Telescope and from the ground. The stellar kinematics measured from these long-slit spectra show rapid rotation (V = 70 km/s at a distance of 0.1 arcsec = 4.9 pc from the nucleus) and increasing velocity dispersion toward the nucleus (where sigma = 295 +/- 30 km/s). We model the observed stellar kinematics assuming an axisymmetric mass distribution with both two and three integrals of motion. Both modeling techniques point to the presence of a central dark compact mass (which presumably is a supermassive black hole) with confidence > 99%. The isotropic two-integral models yield a best-fitting black hole mass of (6.0 +/- 1.4) x 10^7 M_sun and mass-to-light ratio (M/L_V) of 5.38 +/- 0.08, and the goodness-of-fit (chi^2) is insensitive to reasonable values for the galaxy's inclination. The three-integral models, which non-parametrically fit the observed line-of-sight velocity distribution as a function of position in the galaxy, suggest a black hole mass of (3.9 +/- 0.4) x 10^7 M_sun and M/L_V of 5.56 +/- 0.02 (internal errors), and the edge-on models are vastly superior fits over models at other inclinations. The internal dynamics in NGC 1023 as suggested by our best-fit three-integral model shows that the velocity distribution function at the nucleus is tangentially anisotropic, suggesting the presence of a nuclear stellar disk. The nuclear line of sight velocity distribution has enhanced wings at velocities >= 600 km/s from systemic, suggesting that perhaps we have detected a group of stars very close to the central dark mass.Comment: 21 pages, 12 figures, accepted in the Astrophysical Journa