Performance of In Vitro Cassava (Manihot esculenta Crantz) Plantlets Weaned with Locally Sourced Substrates

The performance of in vitro cassava plantlets weaned on different locally sourced substrates was evaluated. Nodal cuttings were excised from healthy six weeks old OG 001cassava variety in the culture room of tissue culture laboratory of National Root Crops Research Institute, Umudike, Abia State, Nigeria. The explants were washed, sterilized and cultured in vitro. The resulting plantlets were weaned on the following substrates - top soil (TS), river sand (RS), saw dust (SD), rice hull waste (RH), 2:1 top soil plus river sand (TS +RS), 2:1 river sand plus saw dust (RS + SD), 2:1 river sand plus rice husk (RS + RH), 2:1 top soil plus saw dust (TP + SD), 2:1 top soil plus rice husk (TP + RH), 2:1 saw dust plus rice husk (SD + RH) and 2:1 peat pellet plus vermiculite (PP + VE), which served as the control. Completely randomized design was used with ten replications. Results showed that plantlets weaned on PP + VE performed better than the other treatments at the end of the weaning period with significantly (P 0.05) highest survival rate (98%), plant vigour (2.6), number of leaves (5) and number of nodes (8). This was closely followed by RS with survival rate, plant vigour, number of leaves and nodes of 63%, 1.4, 1.7 and 3.5, respectively. Plantlets weaned on the other substrates performed poorly. Although plantlets weaned on peat pellet + vermiculite mixture out-performed the other substrates, river sand if properly handled could be a potential substitute for the conventional substrate in weaning cassava plantlets

Time of introducing component crop influences productivity of intercropping system

Field experiment was conducted at National Horticultural Research Institute (NIHORT), Mbato Sub-station, Okigwe, Imo State, South-eastern Nigeria in the 2012 and 2013 cropping seasons to establish the most appropriate time to introduce component crops in cocoyam/cowpea mixture. Five different planting schemes (two and four weeks before, two and four weeks after and same day) and two cowpea genotypes (climbing Akidienu and erect IT97K-499-35) were used. The component crops were grown in monocultures to assess the productivity of the systems. The experimental design used was a completely randomized design with three replicates. Growth and yield of cocoyam and the cowpea genotypes increased significantly (P<0.05) when either of the component crops was planted earlier than the other. Intercropping reduced significantly (P<0.05) cocoyam yield by 0.7 − 74% in IT97K-499-35 and 22 − 80% in Akidienu. Sowing the cowpea genotypes the same day or before cocoyam resulted in over-yielding of cowpea, whereas sowing Akidienu and IT97K-499-35 after cocoyam caused pod yield reductions of 64% − 73% and 32% − 59% on average, respectively. Cocoyam planted two weeks before IT97K-499-35 produced more satisfactory yields of the intercrops than the other planting schedules with LER, LEC and ATER of 2.15, 1.03 and 1.57, respectively

Vacuum-assisted breast biopsy: A comparison of 11-gauge and 8-gauge needles in benign breast disease

© 2008 Hahn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

COVID-19 Transmission due to Mass Mobility Before and After the Largest Festival in Bangladesh : An Epidemiologic Study

Festivals traditionally result in mass public mobility from large cities to rural or semi-urban areas in low- and middle-Income Countries (LMIC), which are inadequately prepared for tackling the consequences of the COVID-19 pandemic. This study aimed to explore the trend of COVID-19 infection in a peripheral region of Bangladesh during one of the largest festivals to develop an evidence-based hypothesis for its influence on the transmission rate of COVID-19. This study conducted a quantitative analysis of secondary data on COVID-19 collected from the Directorate General of Health Services Bangladesh (DGHS) and divisional director’s office in the Mymensingh division. To explore the influence of one of the biggest festivals (Eid-ul-Adha) on the trend of COVID-19 infection, we analyzed data from a week before the festival to 2 weeks following the festival. The infection rate (positive cases per million of the population) and the test positivity rate (positive cases among the total number of conducted diagnostic tests) of each day during this period were calculated both for the Mymensingh region and national level. Both the test positivity rate (TPR) and infection rates in the Mymensingh region demonstrated an increasing trend. The mean test positivity rate of the Mymensingh region on the week before the festival was 9.5%. It increased to a mean test positivity rate of 13% in the following week and further rose to a rate of 17% in the next week. The infection rate of Mymensingh also increased more than 2 folds from the day of the festival (2.0-5.3 cases per million) within the next 2 weeks. The TPR and infection rate on the national level remained similar throughout the study period. Mass mobility during Eid-ul-Adha influences the increased transmission of COVID-19 among the peripheral regions of Bangladesh from the central capital city Dhaka. The findings will help policymakers plan and implement travel restrictions during festivals during the pandemic in LMICs.

Intestinal volvulus in cetaceans

Intestinal volvulus was recognized as the cause of death in 18 cetaceans, including 8 species of toothed whales (suborder Odontoceti). Cases originated from 11 institutions from around the world and included both captive (n = 9) and free-ranging (n = 9) animals. When the clinical history was available (n = 9), animals consistently demonstrated acute dullness 1 to 5 days prior to death. In 3 of these animals (33%), there was a history of chronic gastrointestinal illness. The pathological findings were similar to those described in other animal species and humans, and consisted of intestinal volvulus and a well-demarcated segment of distended, congested, and edematous intestine with gas and bloody fluid contents. Associated lesions included congested and edematous mesentery and mesenteric lymph nodes, and often serofibrinous or hemorrhagic abdominal effusion. The volvulus involved the cranial part of the intestines in 85% (11 of 13). Potential predisposing causes were recognized in most cases (13 of 18, 72%) but were variable. Further studies investigating predisposing factors are necessary to help prevent occurrence and enhance early clinical diagnosis and management of the condition