Soluble adenylyl cyclase mediates hydrogen peroxide-induced changes in epithelial barrier function

BACKGROUND: Elevated H(2)O(2) levels are associated with inflammatory diseases and H(2)O(2) exposure is known to disrupt epithelial barrier function, leading to increased permeability and decreased electrical resistance. In normal human bronchial epithelial (NHBE) cells, fully differentiated at the air liquid interface (ALI), H(2)O(2) activates an autocrine prostaglandin pathway that stimulates transmembrane adenylyl cyclase (tmAC) as well as soluble adenylyl cyclase (sAC), but the role of this autocrine pathway in H(2)O(2)-mediated barrier disruption is not entirely clear. METHODS: To further characterize the mechanism of H(2)O(2)-induced barrier disruption, NHBE cultures were treated with H(2)O(2) and evaluated for changes in transepithelial resistance and mannitol permeability using agonist and inhibitors to dissect the pathway. RESULTS: A short (<10 min) H(2)O(2) treatment was sufficient to induce resistance and permeability changes that occurred 40 min to 1 h later and the changes were partially sensitive to EP1 but not EP4 receptor antagonists. EP1 receptors were localized to the apical compartment of NHBE. Resistance and permeability changes were sensitive to inhibition of sAC but not tmAC and were partially blocked by PKA inhibition. Pretreatment with a PLC inhibitor or an IP3 receptor antagonist reduced changes in resistance and permeability suggesting activation of sAC occurred through increased intracellular calcium. CONCLUSION: The data support an important role for prostaglandin activation of sAC and PKA in H(2)O(2)-induced barrier disruption. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12931-016-0329-4) contains supplementary material, which is available to authorized users

Didactic strategy for the training of skills in clinical trials of Stomatology residents

Introduction: Primary Health Care is the first point of contact that specialists in stomatological sciences provide to the population. Objective: to develop a didactic strategy for training skills in clinical trials for dentistry residents. Method: descriptive, cross-sectional study on the Isle of Youth, 2022-2023. Intentional non-probabilistic sampling was used, with the sample consisting of 39 residents, 20 academic committee professors, 17 provincial coordinators and 7 research methodology professors. Results: a survey was applied to explore knowledge on the topic and it was found that 89,7 % had a low level. The main difficulties identified were in the questions related to knowledge about the researcher\u27s portfolio and the elements necessary to start a clinical trial, with percentages of 7,7 % and 12,8 %, respectively. The proposed strategy was submitted to expert judgment, with scores predominating: very appropriate and moderately appropriate, with values ​​of 64,8 % and 25,6 %, respectively, which represented 90,3 % for the evaluated criteria. The strategy was made up of four stages and actions structured in a coherent manner for its application, which can be enriched from the teaching reflection and the practice of the academic committee of each specialty. Conclusions: the diagnosis of the current situation of the training of Stomatology specialists on the subject of clinical trials revealed potentialities and shortcomings in the conception, the process and the current level of its developmen

Comprehensive care strategy at El Guayabo Penitentiary Center

Introduction: this research is based on the framework of comprehensive oral health care for people deprived of liberty, to ensure that health services ensure their continuity of care, with a quality of care similar to that accessed by the population does not have this limitation. Objective: to develop a comprehensive oral health care strategy at the “El Guayabo” Penitentiary Center on the Isla de la Juventud from October 2021 to October 2022. Methods: an intervention study was carried out on 227 inmates, matching the universe and the sample. The variables were taken into account: age, oral diseases, level of oral health knowledge, oral health knowledge survey and results of the strategy. With prior informed consent, a survey of knowledge of oral health, Stomatological Clinical History, curative and rehabilitative treatment was carried out on each of the patients who were part of the research. Results: before the intervention was applied, dental caries and poor level of knowledge predominated in 71,3 % and 66,9 % of the inmates respectively; managing to reduce the prevalence of dental caries and improve the level of knowledge to be regulated in the majority of inmates after applying the educational strategy; In addition, 47 % of the sample was cured. Conclusions: the implementation of the comprehensive oral health care strategy had a significant impact on the reduction of oral diseases among inmate

Program for the development of digital competencies in teachers of the Stomatology career. Isla de la Juventud

Introduction: information and communication technologies are important tools for training and communication. To facilitate their work in the educational system as a requirement for change in the profile of the education professional, teachers must develop suitable digital skills to implement in their teaching-learning process. Objective: design a program for the development of digital skills in teachers of the Stomatology career of the Faculty of Medical Sciences of the Isla de la Juventud, 2022. Methods: a descriptive study was carried out, from a methodological perspective based on the qualitative paradigm. The universe was made up of 150 teachers, of which 40 made up the sample. The theoretical level research methods were used: analytical-synthetic, inductive-deductive and historical-logical analysis; at the empirical level: documentary analysis, survey and specialist criteria. Results: the need to train the teaching staff and guide the preparation of teachers in the appropriate use of computer techniques and resources, as well as in the preparation and development of virtual courses using the Moodle platform, was confirmed. Conclusions: information and communication technologies are fundamental in the teaching-learning process. The development of digital competencies in teachers shows a low level of performance; the expressed limitations focus mainly on knowledge and domains in content creation, communication and collaboration

Program for the development of digital competencies in teachers of the Stomatology career. Isla de la Juventud

Introduction: information and communication technologies are important tools for training and communication. To facilitate their work in the educational system as a requirement for change in the profile of the education professional, teachers must develop suitable digital skills to implement in their teaching-learning process. Objective: design a program for the development of digital skills in teachers of the Stomatology career of the Faculty of Medical Sciences of the Isla de la Juventud, 2022. Methods: a descriptive study was carried out, from a methodological perspective based on the qualitative paradigm. The universe was made up of 150 teachers, of which 40 made up the sample. The theoretical level research methods were used: analytical-synthetic, inductive-deductive and historical-logical analysis; at the empirical level: documentary analysis, survey and specialist criteria. Results: the need to train the teaching staff and guide the preparation of teachers in the appropriate use of computer techniques and resources, as well as in the preparation and development of virtual courses using the Moodle platform, was confirmed. Conclusions: information and communication technologies are fundamental in the teaching-learning process. The development of digital competencies in teachers shows a low level of performance; the expressed limitations focus mainly on knowledge and domains in content creation, communication and collaboratio

Teenage pregnancy: a social problem for science

Teenage pregnancy has been a social, medical, and educational concern for decades. This phenomenon affects not only the teenager who becomes pregnant, but also her family, her community, and society in general. Over the years, teenage pregnancy rates have varied, but in many countries they remain considerably high, making this problem an urgent social issue. A qualitative study was conducted with the aim of analysing teenage pregnancy as a social problem in science, its repercussions, and the solutions proposed to mitigate its incidence. Empirical (documentary analysis) and theoretical (analytical-synthetic, inductive-deductive, historical-logical analysis, systems approach) methods were used to carry out the research. Teenage pregnancy is a complex social problem involving multiple dimensions, from physical and mental health to education and economics. It is imperative to address this phenomenon with an approach that includes comprehensive sex education, social support, and public health policies tailored to the specific needs of adolescent girls. The educational strategy designed must be multidisciplinary, culturally sensitive, inclusive, and focused on strengthening personal and social capacities for the effective prevention of teenage pregnancy. The future of adolescent girls facing unplanned pregnancies depends on collaboration between governments, educators, health professionals, and communities

Educación superior de calidad: trascendencia e impactos con perspectiva de innovación y sostenibilidad

Higher Education Institutions in countries take on the challenge of continuing to advance in the management of their main university training processes. This could not materialize if they move away from innovative perspectives, approaches and conceptions that promote a culture of quality in university communities as a result of such work. Universities currently focus their management to achieve greater emphasis in their processes and substantive functions, as well as to promote the required impact. Society values the prevailing need for institutions to be accountable for their management, taking into account the processes and contributions they make, as well as the services they provide with the highest quality standards required. The use of theoretical, empirical and statistical-mathematical methods made it possible to systematize learning and good evaluation practices. The objective is to reveal the main results obtained in quality management for the year 2024 as an expression of this culture at Universidad de Oriente. This research reveals the leading role of this university of Excellence, which allows it to fulfill its social mandate, from increasing the levels of quality certification, and in this way, contributes to strengthening a more relevant, prosperous and sustainable Higher Education.La Instituciones de Educación Superior de los países asumen el reto de continuar avanzando en la gestión de sus principales procesos formativos universitarios. Ello no podría materializarse si estos se alejan de perspectivas innovadoras, enfoques y concepciones que fomentan la cultura de calidad en las comunidades universitarias como resultado de dicha labor. Las universidades en la actualidad enfocan su gestión, para lograr mayor realce, en sus procesos y funciones sustantivas, así como propiciar el impacto requerido. La sociedad valora la necesidad imperante de que las instituciones rindan cuenta de su gestión, teniendo en cuenta de los procesos y aportes que estas realizan, así como de los servicios que prestan con los mayores estándares de calidad exigidos. La utilización de métodos teóricos, empíricos y estadísticos-matemáticos posibilitaron la sistematización de los aprendizajes y buenas prácticas evaluativas. Se define como objetivo revelar los principales resultados obtenidos en la gestión de la calidad del año 2024 como expresión de dicha cultura en la Universidad de Oriente. Esta investigación revela el rol protagónico de esta universidad de Excelencia, lo cual permite cumplir su encargo social, a partir de incrementar los niveles de certificación de la calidad, y de este modo, se contribuye a fortalecer una Educación Superior más pertinente, próspera y sostenible

Evaluación de equipo ozonizador doméstico para tratamiento de agua

Los ozonizadores domésticos son equipos de reducido tamaño que pueden ser utilizados para obtener pequeños volúmenes de agua potable de elevada calidad, lo que permite obviar la necesidad de hervir el agua o consumir agua embotellada. En el Centro de Investigaciones del Ozono de Cuba, se diseñó, construyó y evaluó una línea de ozonizadores domésticos que permiten dar una solución local al tratamiento del agua que se va a consumir. En este trabajo, se realizó la evaluación de los principales parámetros que caracterizan el funcionamiento de estos equipos, durante dos etapas de estudio (invierno y verano), para considerar las variaciones de temperatura y humedad propios del clima tropical de Cuba. Esta evaluación incluyó el control de sus condiciones de operación: presión de agua de trabajo, flujos de agua y de gas, tiempo óptimo de funcionamiento continuo y concentraciones de ozono en el gas y en el líquido como parámetros fundamentales, así como la calidad microbiológica del agua obtenida, lo que permitió recomendar las mejores condiciones de operación de estos equipos, que resultaron: presión óptima de agua de entrada al equipo: 1 kg/cm2, flujos de agua: 1 L/min, concentraciones de ozono disueltas en el agua: superior a 0,3 mg/L, tiempo óptimo de funcionamiento continuo: 10 min . Bajo estas condiciones se puede obtener un volumen de 10 L de agua ozonizada, antes de volver a conectar equipo es menester darle 15 min de reposo