T-cadherin upregulation correlates with cell-cycle progression and promotes proliferation of vascular cells

Objective: In vascular tissue, T-cadherin (T-cad) levels correlate with the progression of atherosclerosis, restenosis and tumour neovascularization. This study investigates whether T-cad influences proliferation of vascular cells. Methods and Results: Cultures of human umbilical vein endothelial cells (HUVEC) and rat and human aortic smooth muscle cells (rSMC, hSMC) were used. T-cad was overexpressed in HUVEC and hSMC using an adenoviral expression system. In cultures released from G1/G0 synchrony parallel immunoblot analysis of T-cad and cell cycle phase specific markers (p27Kip1, cyclin D1, E2F1, PCNA, cyclin B) showed increased T-cad protein levels subsequent to entry into early S-phase with sustained elevation through S-and M-phases. T-cad was increased in G2/M-phase (colchicine) synchronized cultures. In FACS-sorted cell populations, expression of T-cad in S-and G2/M-phase was higher than G1/G0-phase. Compared with empty-and LacZ-vector infected controls, HUVEC and hSMC overexpressing T-cad exhibited increased proliferation as assessed in enumeration and DNA synthesis assays. Additionally, following release from G1/G0 synchrony, HUVEC and hSMC overexpressing T-cad enter S-phase more rapidly. Flow cytometry after BrdU/propidium labelling confirmed increased cell cycle progression in T-cad overexpressing cells. Conclusion: In vascular cells, T-cad is dynamically regulated during the cell cycle and its expression functions in the promotion of proliferation. T-cad may facilitate progression of proliferative vascular disorders such as atherosclerosis, restenosis and tumour angiogenesi

Polarisation of T-cadherin to the leading edge of migrating vascular cells in vitro: a function in vascular cell motility?

Both histological and in vitro studies indicate a relationship between T-cadherin levels and acquisition of a modulated, migratory phenotype by vascular cells. This study further examines a role for T-cadherin in relation to cell migration and adhesion. Fluorescence microscopic examination of T-cadherin localisation in confluent cultures of human umbilical vein endothelial cells (HUVEC), human aortic smooth muscle cells and the human carcinoma cell line ECV-304 revealed global distribution over the entire cell body, and with only slight enrichment at cell borders. This contrasts with restricted cell-cell junction localisation of classical cadherin (for example, VE-cadherin in HUVEC). In wounded cultures, T-cadherin polarised to the leading edge of cells migrating into the wound area, again contrasting with classical VE-cadherin, which was undetectable in this region. Confocal microscopy demonstrated that potential signalling functions of T-cadherin at the leading edge are unrelated to physical interactions with caveolin. Adherence of HUVEC onto a monolayer of T-cadherin-transfected L929 cells is significantly reduced compared with adhesion onto control (T-cadherin-negative) L929. Thus T-cadherin is not required for maintenance of intercellular adhesion, but may rather function as a signalling molecule involved in cell-cell recognition and sensing of the environment in processes where cell detachment occur

Metadata for a long-term climate series from the Russian meteorological station “Pyramiden” (1948-1957) at Svalbard ( Short Communication)

Soviet weather station "Pyramiden" was located in the same mining settlement on the northern shore of the Mimer bukta (Billefjorden, West Spitsbergen Island). Research station operated from 1948 to 1957, as a branch of Barentsburg research observatory (Grnfjorden, West Spitsbergen Island). It was the only station that held regular meteorological observations in the inland areas of the archipelago. Observational data (in the form of handwritten books and tables) are preserved in state fond of Arctic and Antarctic Research Institute (AARI, St. Petersburg, Russia). So far, these data have not been digitized and with their help not conducted any climate researches. Fruitful scientific collaboration between MET-Norway (Norwegian Meteorological Institute, Oslo) and AARI helped preserve these unique data to the polar scientific community. Now specialists-climatologists of both institutions complete joint climate researches that in the near future will be available to scientists, studying modern climate changes in the polar regions of the Earth

Sea ice metadata for Billefjorden and Grnfjorden, Svalbard

Description of sea ice conditions in the fjords of Svalbard is crucial for sea transport as well as studies of local climate and climate change. Old observations from the Russian Hydrometeorological stations in the mining settlements Barentsburg (Grnfjorden) and Pyramiden (Billefjorden) have now been digitized. These visual and instrumental observations are archived in the State Archive of Arctic and Antarctic Research Institute (AARI) and Murmansk Branch of the Russian Hydrometeorological Service. In this paper, we bring an overview of the sea ice metadata with few examples of yearly changes in sea ice extent

Theoretical description of hyperpolarization formation in the SABRE-relay method

SABRE (Signal Amplification By Reversible Exchange) has become a widely used method for hyper-polarizing nuclear spins, thereby enhancing their Nuclear Magnetic Resonance (NMR) signals by orders of magnitude. In SABRE experiments, the non-equilibrium spin order is transferred from parahydrogen to a substrate in a transient organometallic complex. The applicability of SABRE is expanded by the methodology of SABRE-relay in which polarization can be relayed to a second substrate either by direct chemical exchange of hyperpolarized nuclei or by polarization transfer between two substrates in a second organometallic complex. To understand the mechanism of the polarization transfer and study the transfer efficiency, we propose a theoretical approach to SABRE-relay, which can treat both spin dynamics and chemical kinetics as well as the interplay between them. The approach is based on a set of equations for the spin density matrices of the spin systems involved (i.e., SABRE substrates and complexes), which can be solved numerically. Using this method, we perform a detailed study of polarization formation and analyze in detail the dependence of the attainable polarization level on various chemical kinetic and spin dynamic parameters. We foresee the applications of the present approach for optimizing SABRE-relay experiments with the ultimate goal of achieving maximal NMR signal enhancements for substrates of interest

THE CONCEPT OF PERSONNEL MANAGEMENT OF THE BANK

Эффективное управление человеческими ресурсами является одним из важнейших условий выживания в конкурентном мире. Ни одна организация не может достичь своих целей без надлежащего управления человеческими ресурсами. Разработка концепции управления персоналом организации ведется с учетом текущих потребностей. Ее составлением занимаются как штатные специалисты, так и привлеченные сторонние эксперты.Целью настоящей работы является определение инструментов и методов стимулирования банковских сотрудников в контексте управления персоналом.Методы исследования: использованы данные интернет-источников, анализ литературы, системный подход и обобщение.Результатом данной работы является определение широкого спектра средств стимулирования сотрудников банковского учреждения в разрезе концепции управления персонала; требований и ориентиров, которым должна соответствовать система управления персоналом в организации.Область применения результатов: полученные результаты могут быть применены в области управления персоналом в банковской сфере. Effective human resource management is one of the most important conditions for survival in a competitive world. No organization can achieve its goals without proper human resource management. The organization’s personnel management concept is being developed taking into account current needs. Both in-house specialists and engaged third-party experts compile it.The purpose of this paper is to study the concept of personnel management on the example of a Bank, to consider the requirements for Bank personnel, and to study methods of stimulating Bank employees in the context of personnel management.Research methods: theoretical analysis and synthesis-the material is taken from the site, a systematic approach and generalization.The result of this work is the definition of a wide range of incentives for employees of a banking institution in the context of the concept of personnel management; requirements and guidelines that should meet the personnel management system in the organizationScope of the results: the results obtained can be applied in the field of personnel management in the banking sector

Features of hemostasis in patients with non-ST-elevation myocardial infarction

Background: Coronary thrombosis is the key pathogenic mechanism of acute heart attack, including non-ST segment elevation (NSTEMI). Given that, the detection of reliable markers of hemostasis disorders is important in the process of optimizing the diagnosis of NSTEMI. Material and methods: The study was conducted on 54 patients with NSTEMI (average age 69.7±1.5 years). In 60% of cases, 3-vessel disease was noted; 56% of patients had ejection fraction >50%, and Killip class I of heart failure was revealed in 78% of patients. With the help of the STA-Liatest (France) equipment, the blood tests determined the following hemostasis markers: fibrin monomers (FM), thrombotic complex activity of factors II, VII and X. Additional markers like Procoag, the coagulation indicator dependent on circulating phospholipids or SPA, D-dimers, as well as factors C, S and antithrombin III were appreciated. The values of these markers determined by the same method in 20 healthy persons (control group) were used as normal values. Results: Circulating level of FM on admission was increased twice, while the values of Procoag and SPA were significantly decreased by 35.3% compared to the control. Factors C, S and antithrombin III were 54-80% of the control value range, and D-dimers were within the permissible values. In the acute phase of the heart attack, a deterioration of hemostasis indicators was noted, excepting the D-dimers. The levels of FM determined 24 and 72 hours after revascularization were consistently increased (up to 3.8 times) compared to the control, while Procoag and SPA decreased by 54-57%. Further reduction of factors C, S and antithrombin III accounted for 42-54% of normal indicators. After 5 days, an improvement in hemostasis markers was observed, but a significant difference still remained comparing to the control group. Conclusions: The hemostasis particularities discovered in patients with NSTEMI indicate the features of an activated prothrombotic status, and FM could be an important diagnostic marker of NSTEMI, due to its most significant deviation from the normal value (>100%). It can reliably reflect the thrombin level, which triggers the last enzymatic phase of thrombus formation