Different Approaches For Protein Engineering In Industrial Biotechnology

Protein engineering is the novel field which has wide applications from pharmaceutics, industry, commercial, laundry and research. It may apply rational design or non rational design or both. Site directed mutagenesis is a classical approach involving the protein folding principles and as such different techniques involving multidisciplinary research and broad knowledge is required involving biocomputing of complex data obtained from various sequencing projects and prediction of the future protein structure either chemically or genetically modified. Non rational mutagenesis or directed evolution involves random mutations in the gene encoding protein or shuffling the genes encoding different domains producing a random set of numerous large libraries of mutant proteins, using advanced technology the desired protein can be selected but the exact structure or changes may remain unnoticed

Molecular Bio-imprinting of Biocatalysts

Energy conservation is the cry of the day. Attempts are made all over the world to occupy and use energy reserves. Increased industrialization and mechanization has led to the depletion of natural energy reserves. Its unavoidable to search for renewable sources of energy, which may be not used now but can be used by future generations. We are using the expertise of our ancestors. Thus exploiting the nature and newer techniques in this area would yield the best results. Bio-imprinting is one of those techniques whereby chemical modification is done in order to achieve highly expressed protein which can be stored in its highly active form in the specific solvent

In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production

According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity, fidelity and generality of the enzyme

Understanding the Qur'an : a reflection

The Qur'an represents the last revelation from on high. It addresses the entire humanity, Muslims and non-Muslims, Jews and Christians, Arabs and non-Arabs, the sincere and the hypocrite, the friends and the opponents, the ypung and the old, the male and the female, the rich and the poor, the educated and the illiterate, the master and the slave, the king and the subject. It seeks to lead them to a blistful life in both the mundane world and in the hereafter. This relationship between the Qur'an and man entails the latter's visiting and understanding the former. But today the man is in a dilemma, knowing not how to grasp the original message of the Qur'an revealed fifteen centuries ago. He finds before him multitudes of Qur'anic commentaries that are, at times mutually conflicting with one another. He is very much keen to take a step forward towards the Qur'an but feels shy rather hesitant to do that due to his lack of confidence to identify a sound methodology of understanding the Qur'an. This book may be deemed as a modest attempts towards the quest for that sound methodology the application of which may ensure true understanding of the Qur'an

Robust vehicle suspension system by converting active and passive control of a vehicle to semi-active control ystem analytically

This research article deals with a simplified translational model of an automotive suspension system which is constructed by considering the translation motion of one wheel of a car. Passive Vehicle Suspension System is converted into Semi Active Vehicle System. Major advantage achieved by this system is that it adjusts the damping of the suspension system without the application of any actuator by using MATLAB® simulations. The semi-active control is found to control the vibration of suspension system very well

Interval-valued fuzzy ideals generated by an interval-valued fuzzy subset in ordered semigroups

In this paper, we de ne the concept of interval-valued fuzzy left (right, two sided, interior, bi-) ideal in ordered semigroups. We show that the interval- valued fuzzy subset J is an interval-valued fuzzy left (right, two sided, interior, bi-) ideal generated by an interval-valued fuzzy subset A i J and J + are fuzzy left (right, two sided, interior, bi-) ideals generated by A and A + respectivelyPeer Reviewe

Study of Compact Stars with Buchdahl Potential in 5-D Einstein-Gauss-Bonnet Gravity

In this paper, we have presented a compact object model in the framework of Einstein-Gauss-Bonnet gravity (EGB) with a linear equation of state considering a metric potential proposed for Buchdahl (1959). The new obtained models satisfy all physical requirements of a physically reasonable stellar object. We analyzed the effect of the Gauss-Bonnet coupling constant {\alpha} on the main physical characteristics of the model. We checked that the radial pressure, energy density and anisotropy are well defined and are regular in the interior of the star and are dependent of the values of the coupling constant.Comment: 22 pages, 14 figure