Improving water productivity, reducing poverty and enhancing equity in mixed crop-livestock systems in the Indo-Gangetic Basin: CPWF project report 68

Farming systems / Mixed farming / Water productivity / Feed production / Livestock / Energy consumption / Gender / Poverty / River basins / Case studies / India / Indo-Gangetic Basin / West Bengal / Haryana / Uttar Pradesh

Human knockouts and phenotypic analysis in a cohort with a high rate of consanguinity

A major goal of biomedicine is to understand the function of every gene in the human genome. Loss-of-function mutations can disrupt both copies of a given gene in humans and phenotypic analysis of such 'human knockouts' can provide insight into gene function. Consanguineous unions are more likely to result in offspring carrying homozygous loss-of-function mutations. In Pakistan, consanguinity rates are notably high. Here we sequence the protein-coding regions of 10,503 adult participants in the Pakistan Risk of Myocardial Infarction Study (PROMIS), designed to understand the determinants of cardiometabolic diseases in individuals from South Asia. We identified individuals carrying homozygous predicted loss-of-function (pLoF) mutations, and performed phenotypic analysis involving more than 200 biochemical and disease traits. We enumerated 49,138 rare (<1% minor allele frequency) pLoF mutations. These pLoF mutations are estimated to knock out 1,317 genes, each in at least one participant. Homozygosity for pLoF mutations at PLA2G7 was associated with absent enzymatic activity of soluble lipoprotein-associated phospholipase A2; at CYP2F1, with higher plasma interleukin-8 concentrations; at TREH, with lower concentrations of apoB-containing lipoprotein subfractions; at either A3GALT2 or NRG4, with markedly reduced plasma insulin C-peptide concentrations; and at SLC9A3R1, with mediators of calcium and phosphate signalling. Heterozygous deficiency of APOC3 has been shown to protect against coronary heart disease; we identified APOC3 homozygous pLoF carriers in our cohort. We recruited these human knockouts and challenged them with an oral fat load. Compared with family members lacking the mutation, individuals with APOC3 knocked out displayed marked blunting of the usual post-prandial rise in plasma triglycerides. Overall, these observations provide a roadmap for a 'human knockout project', a systematic effort to understand the phenotypic consequences of complete disruption of genes in humans.D.S. is supported by grants from the National Institutes of Health, the Fogarty International, the Wellcome Trust, the British Heart Foundation, and Pfizer. P.N. is supported by the John S. LaDue Memorial Fellowship in Cardiology from Harvard Medical School. H.-H.W. is supported by a grant from the Samsung Medical Center, Korea (SMO116163). S.K. is supported by the Ofer and Shelly Nemirovsky MGH Research Scholar Award and by grants from the National Institutes of Health (R01HL107816), the Donovan Family Foundation, and Fondation Leducq. Exome sequencing was supported by a grant from the NHGRI (5U54HG003067-11) to S.G. and E.S.L. D.G.M. is supported by a grant from the National Institutes of Health (R01GM104371). J.D. holds a British Heart Foundation Chair, European Research Council Senior Investigator Award, and NIHR Senior Investigator Award. The Cardiovascular Epidemiology Unit at the University of Cambridge, which supported the field work and genotyping of PROMIS, is funded by the UK Medical Research Council, British Heart Foundation, and NIHR Cambridge Biomedical Research Centre ... Fieldwork in the PROMIS study has been supported through funds available to investigators at the Center for Non-Communicable Diseases, Pakistan and the University of Cambridge, UK

Small and Medium Enterprises' (SMEs) Access to Islamic Banking Finance in Pakistan

This study investigates Small and Medium Enterprises’ (SMEs) access to Islamic banking finance in Pakistan. Despite the significance of SMEs in the economic development of Pakistan, this sector is not shielded from the unavailability of adequate financing facilities. Banks are considered the primary source of financing SMEs all over the globe. However, banks in Pakistan lend to SMEs selectively and, at times, reluctantly. Islamic banks in Pakistan represent only 13% of the whole banking industry lending out of which just 3.7% is attributed to SMEs. It reflects Islamic banks’ unexhausted capabilities to meet a significant part of SMEs’ funding needs in Pakistan. Strong structuration theory was drawn upon through every stage of the research. This study adopts a theoretically informed circular design which consists of the following interlinking parts: a clear objective to investigate the specific research problem, prior theory, theoretically informed research questions, interview questions based on theoretically specific data requirements and thematic data analysis which link the findings to the prior theory (Makrygiannakis and Jack, 2018). The empirical evidence was collected from thirty-five semi-structured interviews with SME owners/managers, Islamic bank managers and a Sharia board member. The findings indicate that significant independent influences are exerted on the activities of SMEs by the external structures. These include Islamic banks, the government of Pakistan, the Federal Board of Revenue (FBR), academic institutions, the State Bank of Pakistan (SBP), Sharia boards and media. SMEs’ internal structures also play a vital role in shaping their practices while accessing Islamic banking finance. These include SME owner/manager’s characteristics, views on Sharia compliance of Islamic banks, preference for different sources of financing, knowledge of Islamic financial products, knowledge of accounting and financial reporting and motives to use Islamic banking finance. These internal and external structures of SMEs seeking to acquire Islamic banking finance interact with each other. As a result of active agency, the on-going patterns of structuration led to the outcomes that incorporate structural reproductions or structural transformations

Enhancing water productivity for poverty alleviation: role of capitals and institutions in the Ganga Basin

The concept of water productivity (WP) or 'more crop per drop' has been revived recently in international water debates. Its application has notably been extended from single crops to mixed farming systems, integrating both crops and livestock, with the wider objective of reducing poverty. Using evidence from the Ganga Basin, India, we discuss the relevance of this concept as a tool to guide interventions for livelihood improvement and poverty alleviation. We argue that WP studies would benefit from greater attention to the role of capitals, inequities and institutions. Firstly, it is crucial to acknowledge the heterogeneity of capitals and capabilities of farmers to make changes in their farming systems and practices and avoid one-fix-all interventions. Identifying pre-existing inequities in water access within and among communities will support better targeting of poor communities. WP interventions can either reinforce or reduce inter-household inequities within communities. We stress the need for assessment of institutional impacts of WP interventions on water access and development

Improving water productivity, reducing poverty and enhancing equity in mixed crop-livestock systems in the Indo-Gangetic Basin: CPWF project report 68

This CGIAR-CPWF Project was designed and conducted by the International Water Management Institute (IWMI), in partnership with international and national partners, to address the relative neglect of livestock water needs of crop-livestock farming systems. The primary objective of this project was to optimize the productive use of water in the crop-livestock farming systems of semi-arid areas to enhance livelihoods, reduce poverty, contribute to gender equity, and protect the environment. This was addressed through an integrated approach led by a multi-disciplinary team across three States of the Ganga Basin

Improving water productivity, reducing poverty and enhancing equity in mixed crop-livestock systems in the Indo-Gangetic Basin

This CGIAR-CPWF Project was designed and conducted by the International Water Management Institute (IWMI), in partnership with international and national partners, to address the relative neglect of livestock water needs of crop-livestock farming systems. The primary objective of this project was to optimize the productive use of water in the crop-livestock farming systems of semi-arid areas to enhance livelihoods, reduce poverty, contribute to gender equity, and protect the environment. This was addressed through an integrated approach led by a multi-disciplinary team across three States of the Ganga Basin