Effects of intake of heated mixtures of glucose-lysine and glucose-methionine on bone calcium

During food thermal treatment Maillard's reaction may occur, which implicates mainly the carbonyl groups of reductory sugars and amino protein groups. Maillard's reaction products (MRP) may interfere with mineral bioavailability because of modifications of their physical-chemical moiety in the food or the lumen, disrupting the absorption process or its normal metabolism. In this study, we sought to investigate MRP influence on issues related to Ca bioavailability in vitro and in vivo. Equimolar mixtures of glucose-lysine (GL) and glucose-methionine (GM) (40% moisture) were heated at 150º C for 90 minutes (samples GL90 and GM90, respectively). In vitro solubility was measured by additioning each samples to a 3.75 mM Ca solution at intestinal pH and ionic strength; after shaking and centrifugation, soluble and insoluble calcium was determined. Three percent of GL90 and GM90 were individually added to the AIN93-G diet to obtain D-GL90 and D-GM90 diets. Three Wistar rats groups were fed for 21 days with both diets and with AIN93-G as control, carrying out calcium balance during last week and extirpating various organs after sacrifice. GM90 did not affect calcium solubility; GL90 reduced it slightly, remaining in both cases more than 94% soluble. D-GL90 and D-GM90 did not modify calcium bioavailability, with as effective usage as with the control diet (57.6 ± 1.3%, 57.8 ± 2.3% and 63.9 ± 2.6% in control diet, D-GL90 and D-GM90, respectively). MRP intake produced, however, metabolic changes that decreased bone calcium, accumulating compensatorily in other organs.Peer reviewe

Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers

A physiological approach to assessing the antioxidant potential of Arbequina EVOO from different zones of Brazil and Spain was performed, applying a combined model of simulated digestion and cell cultures, using the Caco-2 cell line. Our results showed an increasing of total phenolic content (TPC) and antioxidant properties promoted by the in vitro digestion. Preincubating Caco-2 cells with bioaccessible fractions of oils counteracted the cytotoxic effect promoted by an oxidising agent (t-BOOH), preserving cell viability and reducing the generation of reactive oxygen species (ROS). The protective effect on ROS production was associated with the antioxidant activity (DPPH assay), but no relation with the TPC of the digested samples was found. Differences in the parameters evaluated were observed among the samples, which were related to climatic characteristics of the production zones. It was concluded that transformations during the digestive process are important for establishing the antioxidant potential of the oils.We are grateful to the CAPES Foundation, (Ministry of Education, Brazil), for scholarship support provided to the first author (Grant number 6073/13-1). This work is part of the doctoral thesis of Thays Helena Borges, Ph.D. student from the Official Doctoral Program ‘‘Nutrition and Food Sciences” of the Granada University. The authors also would like to thank Consejo Regulador de Denominación de Origen (DOP) Estepa and DOP Les Garrigues; Casas Hualdo, Castillo Canena, Cortijo de Jara, Quaryat Dillara, EPAMIG and Olivas do Sul for the donation of samples. We would like to dedicate to the memory of Carmen Cabrera-Vique.info:eu-repo/semantics/publishedVersio

Application of a potentiometric electronic tongue for assessing phenolic and volatile profiles of Arbequina extra virgin olive oils

The capability of determining the phenolic and volatile profiles of olive oils is of major relevance since these compounds are known to greatly influence the gustatory and olfactory positive attributes of olive oils. An electronic tongue with multiple linear regression models was used to evaluate both profiles based on olive oils potentiometric data generated during a single assay. The proposed electronic tongue-chemometric procedure enabled the quantification of flavonoids, phenolic acids and phenol alcohols of Arbequina extra-virgin olive oils with a similar accuracy of UPLC-MS (0.93 ± 0.03≤R2≤0.98 ± 0.08 for the repeated K-fold cross-validation procedure). Also, it was verified that the potentiometric device should not be applied to evaluate volatile compounds in solution (0.80 ± 0.14≤R2≤0.94 ± 0.05 for the repeated K-fold cross-validation procedure), showing a lower accuracy than HS-SPME-GS-MS. The overall satisfactory results showed that electronic tongue could be used as a practical sensing instrument to generate a chemical profile of the compounds known to influence the positive sensory attributes of olive oils.The authors would like to thank Consejo Regulador de Denominación de Origen (DOP) Estepa and DOP Les Garrigues; Casas Hualdo, Castillo Canena, Cortijo de Jara, Quaryat Dilar, EPAMIG and Olivas do Sul for the donation of samples. We would like to dedicate this work to the memory of Carmen Cabrera-Vique. We are grateful to the CAPES Foundation, (Ministry of Education, Brazil), for scholarship support provided to Thays Helena Borges (grant number 6073/13-1), being presently a Ph.D. student from the Official Doctoral Program ‘‘Nutrition and Food Sciences” of the Granada University. This work was also financially supported by Project POCI-01–0145-FEDER- 006984–Associate Laboratory LSRE-LCM, strategic project PEst-OE/ AGR/UI0690/2014 –CIMO all funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT - Fundação para a Ciência e a Tecnologia, Portugal.info:eu-repo/semantics/publishedVersio

Comparative analysis of minor bioactive constituents (CoQ10, tocopherols and phenolic compounds) in Arbequina extra virgin olive oils from Brazil and Spain

There is currently an emerging production of olive oil in Brazil but it is still poorly characterized. In this study, we performed a comparative analysis of minor bioactive constituents (CoQ 10 tocopherols and phenolic compounds) in extra virgin olive oil from different regions of Brazil and Spain, of Arbequina cultivar. Significant variations (P < 0.05) in the concentration of the compounds analyzed were observed among oils from the different growing areas, not only between Spanish and Brazilian samples but also within zones of the same country. All the oils analyzed showed a high content of CoQ 10 , which ranged from 48 to 85 mg/L. The α − tocopherol was the major isomer quantified and three main groups of phenolic compounds were identified: flavonoids (apigenin, luteolin), phenolic acids (naringenin, p-coumaric acid, vanillic acid) and phenolic alcohols (hydroxytyrosol). Climatic and geographic factors of the production zones greatly influenced the minor fraction composition; positive relationships between altitude and the level of CoQ 10 , tocopherols and phenolic compounds of the oils were observed, whereas a negative correlation with rainfalls was found. Chemometric analyses demonstrated that oils were differentiated by the chemical composition and origin area and that polyphenols (particularly hydroxytyrosol) held the major weight in the oil classification.We are grateful to the CAPES Foundation, (Ministry of Education) Brazil, for scholarship support provided to the first author. LCL is supported by the “Ramón y Cajal” National Programme (RYC-2011- 07643) and the grants SAF2013-47761-R and SAF2015-65786-R, Ministerio de Economía y Competitividad, Spain, and the European Regional Development Fund (ERDF). This work is part of the doctoral thesis of Thays Helena Borges, Ph.D. student from the Official Doctoral Program “Nutrition and Food Sciences” of the Granada University. The authors also would like to thank Consejo Regulador de Denominación de Origen (DOP) Estepa and DOP Les Garrigues; Casas Hualdo, Castillo Canena, Cortijo de Jara, Quaryat Dilar and Olivas do Sul for the donation of samples. We would like to dedicate to the memory of Carmen Cabrera-Vique.info:eu-repo/semantics/publishedVersio

Sodium intake in the diet of a group of adolescents

Los cambios en los hábitos alimentarios de la población han conducido a un incremento en la ingesta de sodio, debido, fundamentalmente, al aumento del consumo de alimentos de fabricación industrial, en detrimento de los preparados en el hogar. Estos cambios se acentúan especialmente en el colectivo adolescente, ya que frecuentemente incluyen en su dieta refrescos, snacks o fast-food, con elevado contenido de sal o aditivos con sodio. Es conocida la relación directa entre la ingesta dietética de sodio y la presión sanguínea, asociándose una ingesta elevada a hipertensión y a enfermedades cardiovasculares. Además, el sodio puede incrementar la eliminación de calcio en la orina, potenciando las pérdidas óseas, por lo que puede ser un factor de riesgo importante para el desarrollo de osteoporosis. En este estudio se realizó una encuesta alimentaria a 21 adolescentes varones de 11- 14 años, con el fin de estimar la ingesta de sodio en su dieta habitual y la contribución de los distintos grupos de alimentos. Para ello se evaluó la ingesta de tres días, mediante recordatorio de 24 horas y registro de ingesta de dos días. Los datos del consumo de alimentos fueron transformados en valores de energía y nutrientes mediante tablas de composición de los alimentos. Los adolescentes consumieron una media de 4558 mg de sodio/día, superando en gran medida las recomendaciones actuales del micronutriente. Las conservas y precocinados fueron los alimentos que proporcionaron el mayor porcentaje del elemento en la dieta (23,5%). Debido al elevado consumo de sodio entre los adolescentes, se recomienda disminuir su ingesta, evitando especialmente un consumo excesivo de alimentos procesados, ricos en sodio. Con esto se pretende prevenir posibles problemas de salud en el futuro, como hipertensión u osteoporosis.Changes in the dietary habits of the population have led to an increase of sodium intake, mainly due to the great intake of manufactured products and the low consumption of homemade foods. These changes are especially important among adolescents, since they frequently include soft drinks, snacks or fast-food in their diets, foods with a high salt content or including sodium-rich additives. It is known the strong relationship between dietary sodium intake and blood pressure: a high sodium intake is related with hypertension and also with cardiovascular diseases. Moreover, sodium intake is associated with increased urinary calcium, therefore increasing bone losses. This fact might be an important risk factor for osteoporosis development. In the present study a dietary questionnaire was realized to 21 male adolescents aged 11-14 years, in order to calculate the sodium intake under their habitual diet and the contribution of the different food sources. The food intake was monitored during a 3-day period, by combining a 24-hour diet recall and two-days weighed dietary record. Data of food consumption were transformed into energy and nutrient vales using tables of food composition. Adolescents consumed an average of 4558 mg/day of sodium, overcoming in a great amount the current recommendations for this micronutrient. Canned and precooked foods contributed the highest percentage of the element in the diet (23.5%). According to these data, it would be extremely advisable to reduce sodium intake among adolescents, avoiding especially excessive processed food consumption, rich in sodium. These actions would be aimed to preventing possible diseases in adulthood such as hypertension and osteoporosis

Composition and Antioxidant Properties of Spanish Extra Virgin Olive Oil Regarding Cultivar, Harvest Year and Crop Stage

The health benefits of extra virgin olive oil (EVOO) are related to its chemical composition and the presence of bioactive compounds with antioxidant properties. The aim of this study was to evaluate antioxidant compounds (pigments, coenzyme Q10 (CoQ10) and phenolic compounds) and antioxidant properties of EVOO from the same region comparing different cultivars (Hojiblanca and Arbequina), harvest year and crop stage. Antioxidant properties of oils were studied before and after a gastrointestinal digestion process, by in vitro assays (DPPH, ABTS and FRAP) and antioxidant markers in Caco-2 cells (reactive oxygen species production). The content of bioactive compounds measured was significantly affected by cultivar and harvest year (except for carotenoids) and by the crop stage (except for coenzyme Q10). Higher amounts of coenzyme Q10 were observed in Hojiblanca than in Arbequina EVOO. Total phenol content and antioxidant properties were also different depending on cultivar and harvest year and the in vitro digestion process strongly improved antioxidant marker values. Antioxidant potential in bioaccessible fractions was mainly related to the content of coenzyme Q10 and phenolic compounds in EVOO. Chemometric analysis showed that the oils were clearly classified by cultivars, harvest and crop stage, according to the chemical composition and antioxidant activity analyzed in the present study

In Vitro Digestion Assays Using Dynamic Models for Essential Minerals in Brazilian Goat Cheeses

Goat cheeses have important nutritional properties, with an emphasis on proteins, lipids (high digestibility), and essential minerals. This study analyzes the bioavailability of Ca, Mg, and Zn in Brazilian cheeses using an in vitro dynamic digestion method. Two self-produced fresh cheeses, cow and goat Minas frescal cheese, and two commercial matured goat cheeses, Blue and Pyramid, were analyzed. Brazilian goat cheeses are potential sources of essential minerals (Ca, Mg, and Zn). Variations of 103–598 mg/100 g for Ca, 13.62–41.64 mg/100 g for Mg, and 9.79–13.23 mg/100 g for Zn were observed in the studied samples. The pH concentration, enzyme performance, and protein and lipid content of Brazilian cheeses affected the solubility of essential minerals in the intestinal fraction. The percentages of minerals found in the permeate stream, equivalent to absorption of Ca and Zn, were lower in Minas frescal goat cheese than Minas frescal cow cheese, whereas that of Mg was higher. Pyramid and Minas frescal goat cheeses had the higher values of Mg and Zn bioavailability, respectively. This study supports, for the first time, the usefulness of the dynamic simulation of the human gastrointestinal tract for the study of mineral bioavailability in cheeses.The authors would like to thank the São Paulo Research Foundation (FAPESP) for the scholarship grant of PhD student José Teixeira, and for the research internship abroad (regular scholarship n° 2018/08864–8 and BEPE—n° 2019/13600–2). Juliana Azevedo Lima Pallone would like to thank the financial support of Brazil (FAPESP 2018/09759–3). The Coordination for the Improvement of Higher Education Personnel (CAPES) (Financial Code 001)

Comparison of body fat percentage assessments by bioelectrical impedance analysis, anthropometrical prediction equations, and dual-energy X-ray absorptiometry in older women

Background: Individuals with high body fat have a higher risk of mortality. Numerous anthropometric-based predictive equations are available for body composition assessments; furthermore, bioelectrical impedance analysis (BIA) estimates are available. However, in older adults, the validity of body fat estimates requires further investigation. Objective: To assess the agreement between percentage body fat (BF%) estimates by BIA and five predictive equations based on anthropometric characteristics using dual X-ray absorptiometry (DXA) as reference method. A secondary objective was to identify whether excluding short-stature women improves the agreement of BF% estimates in a group of community-dwelling, older Mexican women. Methods: A concordance analysis of BF% was performed. A total of 121 older women participated in the study. Anthropometric information, BIA, and DXA body composition estimates were obtained. Five equations using anthropometric data were evaluated in order to determine body fat percentage (BF%) using DXA as reference method. Paired t-test comparisons and standard error of estimates (SEE) were obtained. The Bland-Altman plot with 95% limits of agreement and the concordance correlation coefficient (CCC) were used to evaluate the BF% prediction equations and BIA estimates. Results: The mean age of the study participants was 73.7 ( 5.8) years old. BIA and the anthropometric based equations examined showed mean significant differences when tested in the entire sample. For the taller women (height > 145 cm), no significant difference in the paired comparison was found between DXA and BIA of BF% estimates. The mean BF% was 40.3 ( 4.8) and 40.7 ( 6.2) for DXA and BIA, respectively. The concordance between methods was good (CCC 0.814), (SEE 2.62). Also, in the taller women subset, the Woolcott equation using waist-to-height ratio presented no significant difference in the paired comparison; however, the error of the estimates was high (SEE 3.37) and the concordance was moderate (CCC 0.693). Conclusion: This study found that BIA yielded good results in the estimation of BF% among women with heights over 145 cm. Also, in this group, theWoolcott predictive equation based on waist circumference and height ratio showed no significant differences compared to DXA in the paired comparison; however, the large error of estimates observed may limit its application. In older women, short stature may impact the validity of the body fat percentage estimates of anthropometric-based predictive equations

Growth Potential of Immune- and Surgically Castrated Iberian Pigs Fed Diets of Different Protein Concentratio

Immunocastration is effective to prevent sexual development and boar taint in pigs of conventional genotypes. Other studies pointed out that immunocastrated (IC) pigs show better performance than surgically castrated (SC) pigs. Apart from animal welfare aspects, this fact could be of relevance for Iberian and other fatty pig types with low lean deposition capacity. The purpose of this study was to examine the effects of immunocastration on Iberian pig performance and some carcass traits under different dietary protein concentrations. Fifty-four pure Iberian pigs were used (3 sexes: IC males, IC females, SC males; 3 isoenergetic diets: 160, 140 and 120 g CP/kg DM, 6 pigs/treatment combination). Vaccination against gonadotropin releasing factor was at 4.3 (40 kg) and 6 months of age (70-80 kg). Pigs were individually housed consuming the experimental diets from 40 to 105 kg-BW. The results indicate greater growth rate and feed conversion efficiency for IC males compared to SC males and IC females, and no relevant differences in growth related to dietary protein. Further results will help to elucidate possible effects of immunocastration on lean tissue accretion in Iberian pigs