Blood cytokine profile quantitative characteristics evaluation for identifying infection risk group in pigs

Diseases in newborn animals cause significant damage to animal husbandry. This is a complex problem, in which, along with such factors as the environment and the pathogen, an important role is played by the reaction of the body of newborns and their close connection with the mother's body. The study of enzyme relationships in the functional system «mother-fetus-newborn» can make a significant contribution to solving the problem of improving the safety of the population of newborn animals. Newborn animals have different degrees of functional maturity. Functional capacity of some organs and the system of the newborn, in comparison with the parent individuals, can be determined both genetically and by the conditions of intrauterine development. Currently, a sufficient number of facts have been accumulated that any deviations or violations of homeostasis parameters the mother's body affects the fetus and vice versa. The main role in compensating for impaired functions belongs to the mother's body, but the fetus is also able to participate in these reactions to a certain extent. Functional integration of fetal and maternal homologous systems when performing homeostatic functions concerns the activity of the blood enzyme component. The aim of our research was to study quantitative and qualitative changes in the activity of blood enzymes in animals aged from 27 to 204 days

The Incidence of Cervical Disease in Women of Different Age Groups in the Republic of Sakha (Yakutia)

The purpose of this study was to investigate the incidence of cervical disease in women of different age groups in the Republic of Sakha (Yakutia). Materials and Methods: The cytological material of the cervix of 7,600 women aged between 18 and 88 years was analyzed in the laboratory of pathomorphology, histology and cytology. The material of the cytological study consisted of smears of cervical mucosa and the cervical canal, stained according to the method of Romanovsky-Giemsa. The study was conducted with subjects grouped according to age: Group 1 (18-29), Group 2 (30-44), Group 3 (45-59), and Group 4 (60-74). Results: According to the results of cytological analysis, inflammatory diseases of the cervix uteri were diagnosed in 4,629/61% cases. Among age groups, the highest rate of inflammatory diseases of the cervix uteri was registered in Group 1 and Group 2. Benign cervical lesions were found in 563/7.4% cases with the highest incidence in Groups 1 and 2. The most frequently diagnosed pathology was squamous cell metaplasia with maximum frequency in Group 2 and Group 1. Cervical intraepithelial neoplasia (or dysplasia) (CIN) was detected in 359/4.7% cases. CIN I, CIN II and CIN III were registered in 220/61.3%, 84/24.5%, and 38/10.6% women, respectively. At the same time, the maximum frequency of dysplasia was noted in Group 1 and Group 2. Thus, results obtained indicate a high incidence of cervical disease in women of reproductive age

A Neuron-Glial Perspective for Computational Neuroscience

International audienceThere is growing excitement around glial cells, as compelling evidence point to new, previously unimaginable roles for these cells in information processing of the brain, with the potential to affect behavior and higher cognitive functions. Among their many possible functions, glial cells could be involved in practically every aspect of the brain physiology in health and disease. As a result, many investigators in the field welcome the notion of a Neuron-Glial paradigm of brain function, as opposed to Ramon y Cayal's more classical neuronal doctrine which identifies neurons as the prominent, if not the only, cells capable of a signaling role in the brain. The demonstration of a brain-wide Neuron-Glial paradigm however remains elusive and so does the notion of what neuron-glial interactions could be functionally relevant for the brain computational tasks. In this perspective, we present a selection of arguments inspired by available experimental and modeling studies with the aim to provide a biophysical and conceptual platform to computational neuroscience no longer as a mere prerogative of neuronal signaling but rather as the outcome of a complex interaction between neurons and glial cells

Book Review: Niczyporuk P. Źałova i Powtórne Małźeństwo Wdowy w Prawie Rzymskim. Białystok, 2002. 163 ss.

Рецензируемая монография профессора П. Ничипорука посвящена обязанностям траура вдов и проблеме их возможных повторных браков в римском предклассическом и классическом праве. The monograph of Professor P. Niczyporuk deals with duties of mourning of widows and issue of theirs possible remarriages in the Roman preclassical and classical law

Book Review: Zablocka M. Romanistyka polska po II wojnie swiatowej. Warszawa, 2002. 200 ss.

Рецензия на монографию М. Заблоцкой, содержащей краткий обзор польской литературы по римскому праву, вышедшей в свет в 1945-2001 гг. Review of the monograph M. Zablotskaya, which gives an overview of Polish literature about Roman law, which was published in 1945-2001 years

Excitatory synaptic activity is associated with a rapid structural plasticity of inhibitory synapses on hippocampal CA1 pyramidal cells

Synaptic activity, such as long-term potentiation (LTP), has been shown to induce morphological plasticity of excitatory synapses on dendritic spines through the spine head and postsynaptic density (PSD) enlargement and reorganization. Much less, however, is known about activity-induced morphological modifications of inhibitory synapses. Using an in vitro model of rat organotypic hippocampal slice cultures and electron microscopy, we studied activity-related morphological changes of somatic inhibitory inputs triggered by a brief oxygen-glucose deprivation (OGD) episode, a condition associated with a synaptic enhancement referred to as anoxic LTP and a structural remodeling of excitatory synapses. Three-dimensional reconstruction of inhibitory axo-somatic synapses at different times before and after brief OGD revealed important morphological changes. The PSD area significantly and markedly increased at synapses with large and complex PSDs, but not at synapses with simple, macular PSDs. Activity-related changes of PSD size and presynaptic bouton volume developed in a strongly correlated manner. Analyses of single and serial sections further showed that the density of inhibitory synaptic contacts on the cell soma did not change within 1 h after OGD. In contrast, the proportion of the cell surface covered with inhibitory PSDs, as well as the complexity of these PSDs significantly increased, with less macular PSDs and more complex, segmented shapes. Together, these data reveal a rapid activity-related restructuring of somatic inhibitory synapses characterized by an enlargement and increased complexity of inhibitory PSDs, providing a new mechanism for a quick adjustment of the excitatory-inhibitory balance. This article is part of a Special Issue entitled 'Synaptic Plasticity & Interneurons'

Synaptic potentiation induces increased glial coverage of excitatory synapses in CA1 hippocampus

Patterns of activity that induce synaptic plasticity at excitatory synapses, such as long-term potentiation, result in structural remodeling of the postsynaptic spine, comprising an enlargement of the spine head and reorganization of the postsynaptic density (PSD). Furthermore, spine synapses represent complex functional units in which interaction between the presynaptic varicosity and the postsynaptic spine is also modulated by surrounding astroglial processes. To investigate how activity patterns could affect the morphological interplay between these three partners, we used an electron microscopic (EM) approach and 3D reconstructions of excitatory synapses to study the activity-related morphological changes underlying induction of synaptic potentiation by theta burst stimulation or brief oxygen/glucose deprivation episodes in hippocampal organotypic slice cultures. EM analyses demonstrated that the typical glia-synapse organization described in in vivo rat hippocampus is perfectly preserved and comparable in organotypic slice cultures. Three-dimensional reconstructions of synapses, classified as simple or complex depending upon PSD organization, showed significant changes following induction of synaptic potentiation using both protocols. The spine head volume and the area of the PSD significantly enlarged 30 min and 1 h after stimulation, particularly in large synapses with complex PSD, an effect that was associated with a concomitant enlargement of presynaptic terminals. Furthermore, synaptic activity induced a pronounced increase of the glial coverage of both pre- and postsynaptic structures, these changes being prevented by application of the NMDA receptor antagonist D-2-amino-5-phosphonopentanoic acid. These data reveal dynamic, activity-dependent interactions between glial processes and pre- and postsynaptic partners and suggest that glia can participate in activity-induced structural synapse remodeling

Ischemia-induced modifications in hippocampal CA1 stratum radiatum excitatory synapses

Relatively mild ischemic episode can initiate a chain of events resulting in delayed cell death and significant lesions in the affected brain regions. We studied early synaptic modifications after brief ischemia modeled in rats by transient vessels' occlusion in vivo or oxygen-glucose deprivation in vitro and resulting in delayed death of hippocampal CA1 pyramidal cells. Electron microscopic analysis of excitatory spine synapses in CA1 stratum radiatum revealed a rapid increase of the postsynaptic density (PSD) thickness and length, as well as formation of concave synapses with perforated PSD during the first 24 h after ischemic episode, followed at the long term by degeneration of 80% of synaptic contacts. In presynaptic terminals, ischemia induced a depletion of synaptic vesicles and changes in their spatial arrangement: they became more distant from active zones and had larger intervesicle spacing compared to controls. These rapid structural synaptic changes could be implicated in the mechanisms of cell death or adaptive plasticity. Comparison of the in vivo and in vitro model systems used in the study demonstrated a general similarity of these early morphological changes, confirming the validity of the in vitro model for studying synaptic structural plasticity

Blood cytokine profile quantitative characteristics evaluation for identifying infection risk group in pigs

Diseases in newborn animals cause significant damage to animal husbandry. This is a complex problem, in which, along with such factors as the environment and the pathogen, an important role is played by the reaction of the body of newborns and their close connection with the mother's body. The study of enzyme relationships in the functional system «mother-fetus-newborn» can make a significant contribution to solving the problem of improving the safety of the population of newborn animals. Newborn animals have different degrees of functional maturity. Functional capacity of some organs and the system of the newborn, in comparison with the parent individuals, can be determined both genetically and by the conditions of intrauterine development. Currently, a sufficient number of facts have been accumulated that any deviations or violations of homeostasis parameters the mother's body affects the fetus and vice versa. The main role in compensating for impaired functions belongs to the mother's body, but the fetus is also able to participate in these reactions to a certain extent. Functional integration of fetal and maternal homologous systems when performing homeostatic functions concerns the activity of the blood enzyme component. The aim of our research was to study quantitative and qualitative changes in the activity of blood enzymes in animals aged from 27 to 204 days