Lessons learned from the past: forestry initiatives for effective carbon stocking in Southern Italy

Calabria (Italy) is a particularly interesting region of the Mediterranean basin from the perspective of forest management due to the extension of reforestation activities aimed at soil conservation. According to international agreements, these reforestation activities fulfill other functions as well, including carbon storage. Thus, Calabria was selected as a representative area for a study on the different typologies of forest plantations to verify the effects of these functions. Results showed a significant increment in carbon stock compared to the previous land use (i.e. arable land and pastures) and how the average carbon stock per hectare varies in relation to the species considered at the above- and below-ground levels. Carbon stock was higher in conifers (Calabrian pine, Douglas fir) and lower in broad-leaved trees (Turkey oak, European chestnut). The study analyses demonstrate how, based on different intensities of thinning, the carbon eliminated by trees is reconstituted over time in quantities larger than those eliminated by cutting. This latter aspect is relevant, as forest management allows the partial removal of biomass produced without negatively affecting carbon stock. Consequently, reforestation and sustainable forms of forest management are powerful strategies for mitigating the effects of climate change

Prep1 Controls Insulin Glucoregulatory Function in Liver by Transcriptional Targeting of SHP1 Tyrosine Phosphatase

AbstractObjective. We have investigated the function of the Prep1 gene in insulin-dependent glucose homeostasis in liver. Research design and methods. Prep1 action on insulin glucoregulatory function has been analyzed in liver of Prep1-hypomorphic mice (Prep1(i/i)), which express 2 to 3% of Prep1 mRNA. Results. Based on euglycemic hyperinsulinemic clamp studies, pyruvate tolerance tests and measurement of glycogen content, livers from Prep1(i/i) mice feature increased sensitivity to insulin. Tyrosine phosphorylation of both insulin receptor (IR) and IRS1/2 was significantly enhanced in Prep1(i/i) livers accompanied by a specific down-regulation of the SYP and SHP1 tyrosine phosphatases. Prep1 overexpression in HepG2 liver cells upregulated SYP and SHP1 and inhibited insulin-induced IR and IRS1/2 phosphorylation and was accompanied by reduced glycogen content. Consistently, overexpression of the Prep1 partner Pbx1 but not of p160MBP, mimicked Prep1 effects on tyrosine phosphorylations, glycogen content and on SYP and SHP1 expression. In Prep1 overexpressing cells, antisense silencing of SHP1, but not that of SYP, rescued insulin-dependent IR phosphorylation and glycogen accumulation. Both Prep1 and Pbx1 bind SHP1 promoter at a site located between nt -2113 and -1778. This fragment features enhancer activity and induces luciferase function by 7, 6 and 30-fold, respectively, in response to Prep1, Pbx1 or both. Conclusions. SHP1, a known silencer of insulin signal, is a transcriptional target of Prep1. In liver, transcriptional activation of SHP1 gene by Prep1 attenuates insulin signal transduction and reduces glucose storage

Tagging genes with cassette-exchange sites

In an effort to make transgenesis more flexible and reproducible, we developed a system based on novel 5′ and 3′ ‘gene trap’ vectors containing heterospecific Flp recognition target sites and the corresponding ‘exchange’ vectors allowing the insertion of any DNA sequence of interest into the trapped locus. Flp-recombinase-mediated cassette exchange was demonstrated to be highly efficient in our system, even in the absence of locus-specific selection. The feasibility of constructing a library of ES cell clones using our gene trap vectors was tested and a thousand insertion sites were characterized, following electroporation in ES cells, by RACE–PCR and sequencing. We validated the system in vivo for two trapped loci in transgenic mice and demonstrated that the reporter transgenes inserted into the trapped loci have an expression pattern identical to the endogenous genes. We believe that this system will facilitate in vivo studies of gene function and large-scale generation of mouse models of human diseases, caused by not only loss but also gain of function alleles

Dinamiche dei popolamenti del pino d'Aleppo negli alvei delle fiumare dell'Alto Ionio cosentino

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A relocatable ocean modelling platform for downscaling to shelf-coastal areas to support disaster risk reduction

High-impact ocean weather events and climate extremes can have devastating effects on coastal zones and small islands. Marine Disaster Risk Reduction (DRR) is a systematic approach to such events, through which the risk of disaster can be identified, assessed and reduced. This can be done by improving ocean and atmosphere prediction models, data assimilation for better initial conditions and developing an efficient and sustainable impact forecasting methodology for Early Warnings Systems. A common user request during disaster remediation actions is for high-resolution information, which can be derived from easily deployable numerical models nested into operational larger-scale ocean models. The Structured and Unstructured Relocatable Ocean Model for Forecasting (SURF) enables users to rapidly deploy a nested high-resolution numerical model into larger-scale ocean forecasts. Rapidly downscaling the currents, sea level, temperature, and salinity fields is critical in supporting emergency responses to extreme events and natural hazards in the world’s oceans. The most important requirement in a relocatable model is to ensure that the interpolation of low-resolution ocean model fields (analyses and reanalyses) and atmospheric forcing is tested for different model domains. The provision of continuous ocean circulation forecasts through the Copernicus Marine Environment Monitoring Service (CMEMS) enables this testing. High-resolution SURF ocean circulation forecasts can be provided to specific application models such as oil spill fate and transport models, search and rescue trajectory models, and ship routing models requiring knowledge of meteooceanographic conditions. SURF was used to downscale CMEMS circulation analyses in four world ocean regions, and the high-resolution currents it can simulate for specific applications are examined. The SURF downscaled circulation fields show that the marine current resolutions affect the quality of the application models to be used for assessing disaster risks, particularly near coastal areas where the coastline geometry must be resolved through a numerical grid, and high-frequency coastal currents must be accurately simulated

STAND STRUCTURE OF A CALABRIAN PINE OLD-GROWTH FOREST: INDICATIONS FOR FOREST MANAGEMENT AND LANDSCAPE CONSERVATION

Calabrian pine forests are the peculiar feature of the landscape of many Italian mountain areas: Etna, Aspromonte and, especially, Sila. Sila landscape conservation is important not only for cultural and historical heritage value, but also for environmental and socioeconomic aspects. This is particularly relevant as most Calabrian pine forests are now included in the Sila National Park. The authors analyze the structure of an old growth pine forest. The main structural characters are compared with those of small group selection pine forests. Results show that small group selection felling, a tradidional silvicultural system peculiar to this area for Calabrian pine, is the best silvicultural approach for the conservation of Calabrian pine forests

Gestione forestale e prevenzione del dissesto idrogeologico in territori ad elevata vulnerabilità in Campania

This paper focuses on the compatibility between forest management and soil conservation with reference to chesnut (Castanea sativa Mill.), holm oak (Quercus ilex L.) and especially Manna Ash (Fraxinus ornus L.) mixed hop Hornbeam (Ostrya carpinifolia Scop.) coppices stands, located on slopes of Campania and characterized by intensely pedogenised pyroclastic coverage, but unstable when facing intense rainy events. Based on the Corine Land Cover system (2012), we divided the areas into the three physiognomic types; thus, the proportion of surfaces falling under the pyroclastic coverage, which are the more instable, were obtained by overlapping the land systems and soil map produced by the Campania region (2014). For each type of coppice, we examined all the different structural conditions (traditional coppices, coppices past traditional rotation age and coppice in conversion to high forest) and underlined the most important critical issues due to clearcutting, We summarized the different types of silvicultural interventions to improve the forest management sustainability, especially in those areas which are vulnerable to hydrogeological instability. We also stressed that forest planning is the essential tool for sustainable management of coppices, together with the catchment area and the fire-fighting planning

Phorbol Esters Induce Intracellular Accumulation of the Anti-apoptotic Protein PED/PEA-15 by Preventing Ubiquitinylation and Proteasomal Degradation

Phosphoprotein enriched in diabetes/phosphoprotein enriched in astrocytes (PED/PEA)-15 is an anti-apoptotic protein whose expression is increased in several cancer cells and following experimental skin carcinogenesis. Exposure of untransfected C5N keratinocytes and transfected HEK293 cells to phorbol esters (12-O-tetradecanoylphorbol-13-acetate (TPA)) increased PED/PEA-15 cellular content and enhanced its phosphorylation at serine 116 in a time-dependent fashion. Ser-116 --> Gly (PED(S116G)) but not Ser-104 --> Gly (PED(S104G)) substitution almost completely abolished TPA regulation of PED/PEA-15 expression. TPA effect was also prevented by antisense inhibition of protein kinase C (PKC)-zeta and by the expression of a dominant-negative PKC-zeta mutant cDNA in HEK293 cells. Similar to long term TPA treatment, overexpression of wild-type PKC-zeta increased cellular content and phosphorylation of WT-PED/PEA-15 and PED(S104G) but not of PED(S116G). These events were accompanied by the activation of Ca2+-calmodulin kinase (CaMK) II and prevented by the CaMK blocker, KN-93. At variance, the proteasome inhibitor lactacystin mimicked TPA action on PED/PEA-15 intracellular accumulation and reverted the effects of PKC-zeta and CaMK inhibition. Moreover, we show that PED/PEA-15 bound ubiquitin in intact cells. PED/PEA-15 ubiquitinylation was reduced by TPA and PKC-zeta overexpression and increased by KN-93 and PKC-zeta block. Furthermore, in HEK293 cells expressing PED(S116G), TPA failed to prevent ubiquitin-dependent degradation of the protein. Accordingly, in the same cells, TPA-mediated protection from apoptosis was blunted. Taken together, our results indicate that TPA increases PED/PEA-15 expression at the post-translational level by inducing phosphorylation at serine 116 and preventing ubiquitinylation and proteosomal degradation