Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii

Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period of 12 months when stored at −70°C using dimethyl sulfoxide (DMSO; 5 or 10%) as cryopreservant. As A. castellanii are naturally capable of encystment, studies were undertaken to determine whether induced encystment might improve the viability of organisms under a number of storage conditions. A. castellanii cysts stored in the presence of Mg2+ at 4°C remained viable over the study period, although time to confluence was increased from approximately 8 days to approximately 24 days over the 12-month period. Storage of cysts at −70°C with DMSO (5 or 10%) or 40% glycerol, but not 80% glycerol as cryopreservants increased their viability over the 12-month study period compared with those stored at room temperature. Continued presence of Mg2+ in medium during storage had no adverse effects and generally improved recovery of viable organisms. The present study demonstrates that A. castellanii can be stored as a non-multiplicative form inexpensively, without a need for cryopreservation, for at least 12 months, but viability is increased by storage at −70°C

Rural water collection patterns: combining smart meter data with user experiences in Tanzania (article)

This is the final version. Available on open access from MDPI via the DOI in this recordThe dataset associated with this article is available in ORE at: https://doi.org/10.24378/exe.2283Water collection from piped water distribution systems (PWSs) in rural communities in sub-Saharan Africa is not understood in much detail. Here, distances travelled to distribution points (DPs), volumes collected, times of day of collection, and the relationship between location of DP and volume dispensed are investigated in a rural community in Tanzania using a combination of novel smart pre-payment meters and results from surveys and interviews. Continuous availability of water through pre-payment smart meters is shown to eliminate queue time, freeing time for farming or schooling, and enhance revenue collection for service provision. 97% of users use DPs as a main source of drinking water as opposed to unimproved alternative sources, and 42% live further than 400 meters from a DP. Collection occurs across daylight with greater volumes dispensed in the afternoon-evening due to free time from economic activities and children returning from school. A low mean daily collection of 47 litres per household from DPs is measured. Volume dispensed across different DPs over time shows no clear pattern. However, the volume dispensed is indicated by the number of households that use each DP. Increasing PWS capacity can accommodate for variable collection patterns, and help improve sustainability of rural water supply.Africa Water EnterprisesEngineering and Physical Sciences Research Council (EPSRC

New techniques for imaging and analyzing lung tissue.

The recent technological revolution in the field of imaging techniques has provided pathologists and toxicologists with an expanding repertoire of analytical techniques for studying the interaction between the lung and the various exogenous materials to which it is exposed. Analytical problems requiring elemental sensitivity or specificity beyond the range of that offered by conventional scanning electron microscopy and energy dispersive X-ray analysis are particularly appropriate for the application of these newer techniques. Electron energy loss spectrometry, Auger electron spectroscopy, secondary ion mass spectrometry, and laser microprobe mass analysis each offer unique advantages in this regard, but also possess their own limitations and disadvantages. Diffraction techniques provide crystalline structural information available through no other means. Bulk chemical techniques provide useful cross-checks on the data obtained by microanalytical approaches. It is the purpose of this review to summarize the methodology of these techniques, acknowledge situations in which they have been used in addressing problems in pulmonary toxicology, and comment on the relative advantages and disadvantages of each approach. It is necessary for an investigator to weigh each of these factors when deciding which technique is best suited for any given analytical problem; often it is useful to employ a combination of two or more of the techniques discussed. It is anticipated that there will be increasing utilization of these technologies for problems in pulmonary toxicology in the decades to come

Temperature controlled material irradiation in the advanced test reactor

The Advanced Test Reactor (ATR) is located at the Idaho National Engineering Laboratory (INEL) near Idaho Falls, Idaho, USA and is owned and regulated by the U.S. Department of Energy (US DOE). The ATR is operated for the US DOE by Lockheed Martin Idaho Technologies. In recent years, prime irradiation space in the ATR has been made available for use by customers having irradiation service needs in addition to the reactor`s principal user, the U.S. Naval Nuclear Propulsion Program. To enhance the reactor`s capabilities, the US DOE has initiated the development of an Irradiation Test Vehicle (ITV) capable of providing neutron spectral tailoring and temperature control for up to 28 experiments. The ATR-ITV will have the flexibility to simultaneously support a variety of experiments requiring fast, thermal or mixed spectrum neutron environments. Temperature control is accomplished by varying the thermal conductivity across a gas gap established between the experiment specimen capsule wall and the experiment `in-pile tube (IPT)` inside diameter. Thermal conductivity is adjusted by alternating the control gas mixture ratio of two gases with different thermal conductivities

Reconstruction Control of Magnetic Properties during Epitaxial Growth of Ferromagnetic Mn_3-δGa on Wurtzite GaN(0001)

Binary ferromagnetic Mn_3-δGa (1.2<3-δ≤1.5) crystalline thin films have been epitaxially grown on wurtzite GaN(0001) surfaces using rf N-plasma molecular beam epitaxy. The film structure is face-centered tetragonal with CuAu type-I (L1_0) ordering with (111) orientation. The in-plane epitaxial relationship to GaN is nearly ideal with [11̅ 0]_MnGa∥[11̅ 00]_GaN and [112̅ ]_MnGa∥[112̅ 0]_GaN. We observe magnetic anisotropy along both the in-plane and out-of-plane directions. The magnetic moments are found to depend on the Mn/(Mn+Ga) flux ratio and can be controlled by observation of the surface reconstruction during growth, which varies from 1×1 to 2×2 with increasing Mn stoichiometry

Hungry for change: the Sydney Food Fairness Alliance

The Sydney Food&nbsp;Fairness&nbsp;Alliance is one of a growing number of nascent food movements in Australia to have emerged out of concern for the country’s food future, as well as the deleterious effect the present food system is having on its citizens’ health and the continent’s fragile environment. The Alliance’s structure and activities clearly position it as a new social movement (NSM) engaged in collective action on a specific issue, in this instance, food security/justice, and operating outside the political sphere while aiming to influence and affect societal change.&nbsp;Food security&nbsp;as a human right lies at the heart of the Alliance’s philosophy, and equitable, sustainable food policies for&nbsp;New South Wales&nbsp;are a core focus of its advocacy work. The authors argue that the Alliance is a distinctive food movement in that it positions itself as an \u27umbrella\u27 organization representing a wide range of stakeholders in the food system. This chapter reflects on the values, achievements, issues of concern, strengths and weaknesses, and future of the Sydney Food&nbsp;Fairness Alliance. This resource is Chapter 8 in \u27Food Security in Australia:&nbsp;Challenges and Prospects for the Future\u27 published by Springer in 2013

Health Orientation, Beliefs, and Use of Health Services Among Minority, High-risk Expectant Mothers

This article reports on initial findings of a continuing longitudinal study investigating the relationships of health beliefs as conceptualized by the health belief model and the use of well-baby services among first-time black mothers. The health beliefs of mothers about their babies were measured before the babies were born and during their use of the services at the baby's first and sixth-month visits. Mothers in the sample who became nonusers of the well-baby services were also interviewed. This report describes the results of the first interview of the 662 females who composed the sample for the study, including the following characteristics of a minority, high-risk population: health orientation, health beliefs about their unborn babies, and use of health services. These findings are discussed with implications for community health nursing practice with maternal clients.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73774/1/j.1525-1446.1988.tb00553.x.pd

Mesoporous stilbene-based lanthanide metal organic frameworks: synthesis, photoluminescence and radioluminescence characteristics

Mesoporous non-interpenetrating stilbene-based lanthanide metal organic frameworks exhibits photo and radioluminescence behavior.</p

Non exponential relaxation in fully frustrated models

We study the dynamical properties of the fully frustrated Ising model. Due to the absence of disorder the model, contrary to spin glass, does not exhibit any Griffiths phase, which has been associated to non-exponential relaxation dynamics. Nevertheless we find numerically that the model exhibits a stretched exponential behavior below a temperature T_p corresponding to the percolation transition of the Kasteleyn-Fortuin clusters. We have also found that the critical behavior of this clusters for a fully frustrated q-state spin model at the percolation threshold is strongly affected by frustration. In fact while in absence of frustration the q=1 limit gives random percolation, in presence of frustration the critical behavior is in the same universality class of the ferromagnetic q=1/2-state Potts model.Comment: 7 pages, RevTeX, 11 figs, to appear on Physical Review