Post‐budburst hand finishing of winter spur pruning can delay technological ripening without altering phenolic maturity of Merlot berries

Background and Aims Global warming can lead to technological ripening occurring in advance of phenolic maturity for red wine cultivars. This study evaluated the effect of post‐budburst winter pruning on the phenology, yield components, berry composition and phenolic maturity in Vitis vinifera L. cv. Merlot. Methods and Results Mechanically pre‐pruned vines followed up with hand pruning during winter (Control) were compared to vines that were mechanically pre‐pruned and followed up with hand pruning after budburst when distal buds developed shoots with either three unfolded leaves (DF3) or eight unfolded leaves (DF8). Late pruning delayed budburst, flowering and, to a lesser extent, veraison. The delays were greater for DF8 than DF3 treatments. Yield decreased by about 40 and 71% in DF3 and DF8 vines, respectively, while sugar accumulation and reduction of TA were delayed by both pruning treatments. The concentration of anthocyanin and tannin and of extractable anthocyanin and tannin (of skin and seeds analysed separately) were not influenced by the DF3 treatment while tannin concentration increased in DF8 berries. Conclusions Delaying hand pruning of mechanically pre‐pruned vines until after budburst of distal nodes can delay technological ripening without affecting the concentration of anthocyanin and tannin of berries. Yield, however, is substantially reduced. Significance of the Study We verified the feasibility of a cost‐effective technique that can be adopted to counteract the hastening of sugar accumulation and organic acid decline caused by global warming on valuable black grapes

Vine Physiology, Yield Parameters and Berry Composition of Sangiovese Grape under Two Different Canopy Shapes and Irrigation Regimes

Vitis vinifera L. adapts well to a scarce availability of water in the soil. However, in recent decades, the combination of thermal stress with prolonged water scarcity could have dramatic consequences on the vine’s physiological status. In this paper, we evaluated the effects of two canopy shapes and two irrigation regimes at veraison on vine physiology, yield parameters and grape composition through biochemical and molecular approaches. The water shortage strongly influenced the physiology of Sangiovese only when the stress was moderate to severe. Neither the water stress limited to veraison nor the canopy shape were able to influence the yield parameters and sugar content, and a strong induction of the expression of the genes involved in the biosynthesis of anthocyanins was recorded only in conditions of moderate-to-severe stress. This phenomenon led to an increase in the anthocyanin content in berry skins until the end of veraison. Conversely, no significant effects occurred in terms of biochemical and molecular performance after re-watering and at harvest. Though the shape of the canopy could play a role only under elevated temperature and prolonged drought, severe water stress can affect the vine physiology and berry ripening during the veraison stag

Late trimming delays sugar accumulation in grapes

Background and Aims Climate change can alter the synchronous accumulation of sugar and other main berry compounds during ripening. The aim of this study was to determine whether post-veraison trimming could delay sugar accumulation and influence the production of anthocyanins and seed tannins in Sangiovese grapes. Methods and Results Shoots were trimmed in 2009, 2010 and 2011 when the berry total soluble solids (TSS) reached 15–17°Brix, leaving eight nodes on each main shoot. The accumulation of TSS, anthocyanins and seed tannins was measured during ripening, and yield parameters were recorded at harvest. Grapes from trimmed vines contained a lower TSS in 2009 and 2010, but there was no impact on the concentration of anthocyanins and seed tannins. In 2011, leaf area limitation was insufficient to reduce TSS accumulation, because yield constraints were observed and the leaf area/yield ratio was within the optimal range. Conclusions The lower rate of TSS accumulation in berries had no impact on the concentration of anthocyanins and seed tannins, suggesting that this approach could produce grapes with a lower TSS at harvest or delay harvest. In low-vigour vines suffering from water deficit, the post-veraison trimming repeated over the years could reduce yield, which may be responsible for the lack of TSS reduction compared with that of control vines. Significance of the Study Our study provides insight into the relationship between TSS accumulation and the production of anthocyanins and seed tannins in the berry in response to post-veraison leaf area reduction

Lung involvement in "stable" undifferentiated connective tissue diseases: a rheumatology perspective

Previous studies of the occurrence of interstitial lung disease (ILD) in undifferentiated connective tissue diseases (UCTD) were conducted in patients admitted to Respiratory Medicine Units. The aim of the present prospective study was to investigate lung involvement in UCTD patients admitted to a Rheumatology Unit. Eighty-one consecutive UCTD patients were enrolled in the study. Each patient underwent history and physical examination, routine laboratory investigations, antinuclear antibody (ANA) profiling, B-mode echocardiography, and lung function study according to previously reported methods. Lung high resolution computed tomography (HRCT) was performed in patients who provided informed consent. Six patients (7.4%) had a history of grade II dyspnea. Three of them had a DLCO ranging from 42 to 55% of the predicted value; and a HRCT-documented ILD with a non-specific interstitial pneumonia (NSIP) pattern. Symptoms in the other three patients were due to cardiac disease. None of the 75 asymptomatic patients, had relevant findings at physical examination, 26/75 had a DLCO <80% (<70% in 10 cases). Of these, 3 of the 30 patients who underwent lung HRCT were affected by NSIP-ILD. Six of the 81 enrolled were affected by ILD, which was symptomatic in three patients. A higher percentage of patients had a reduced DLCO. The latter finding may reflect a preradiographic ILD or a preechocardiographic pulmonary vascular disease

Determinants of Noninvasive Ventilation Outcomes during an Episode of Acute Hypercapnic Respiratory Failure in Chronic Obstructive Pulmonary Disease: The Effects of Comorbidities and Causes of Respiratory Failure

Objectives. To investigate the effect of the cause of acute respiratory failure and the role of comorbidities both acute and chronic on the outcome of COPD patients admitted to Respiratory Intensive Care Unit (RICU) with acute respiratory failure and treated with NIV. Design. Observational prospective study. Patients and Methods. 176 COPD patients consecutively admitted to our RICU over a period of 3 years and treated with NIV were evaluated. In all patients demographic, clinical, and functional parameters were recorded including the cause of acute respiratory failure, SAPS II score, Charlson comorbidity index, and further comorbidities not listed in the Charlson index. NIV success was defined as clinical improvement leading to discharge to regular ward, while exitus or need for endotracheal intubation was considered failure. Results. NIV outcome was successful in 134 patients while 42 underwent failure. Univariate analysis showed significantly higher SAP II score, Charlson index, prevalence of pneumonia, and lower serum albumin level in the failure group. Multivariate analysis confirmed a significant predictive value for pneumonia and albumin. Conclusions. The most important determinants of NIV outcome in COPD patients are the presence of pneumonia and the level of serum albumin as an indicator of the patient nutritional status

Climate change and vine training systems: the influence different spatial distribution of shoots may have on sugar accumulation in Sangiovese grapevines.

The choice of training system may influence vineyard efficiency in terms of light interception and water consumption, particularly in the current context of climate change. On this basis, during the 2017 season, Sangiovese potted vines were grown outdoors using two different training systems: guyot vertical shoot positioned system (C) and V-shaped open canopy (A). From the end of June until September, vine transpiration was continuously monitored by the gravimetric approach and at different times in the season, the leaf area, light interception, photosynthetic activity and stem water potential were measured. Grape yield and fruit composition were recorded at harvest. C plants did not differ from A in terms of leaf area during the entire season. Light interception was higher in C vines during the early hours of the morning and lower in the central part of the day and the transpiration loss was higher, as was the net photosynthesis detected on some days in August. No differences were detected in terms of yield but a significant increase in soluble solids was found in C compared to A. The open canopy compared to a closed one, in a particularly hot year like 2017, resulted in negative consequences on the accumulation of soluble solids, probably as a result of the radiative stress suffered by the A vines in the middle hours of the day

ANTEPARTUM DISTRESS DURING COVID-19 PANDEMIC: AN OBSERVATIONAL STUDY

Background: The present study investigates the impact of the Coronavirus diseases 2019 (Covid-19) pandemic on the subjective experience of pregnant women, as well as the impact of the pandemic on this population in terms of psychopathological correlates. Subjects and methods: Pregnant women referring to the Section of Obstetrics and Gynecology of the General Hospital of Perugia, Italy, were recruited from 1st May, 2021 to 15th June, 2021. Socio-demographic and clinical data was collected, as well as information regarding the Covid-19 pandemic impact on the subjective experience of pregnancy. Psychopathology was evaluated by means of the State-Trait Anxiety Inventory Form Y (STAI-Y), the Symptom Checklist-90 (SCL-90) and the Prenatal Distress Measure (Pre-DM). Descriptive analyses were performed. Significant associations between distress symptoms and the collected sociodemographic and clinical variables were assessed by using the Pearson correlation (p<0.05). Results: 25 women were included in the study. Among these, 18 (72%) reported that the Covid-19 pandemic negatively impacted their experience of pregnancy. Were detected an average Pre-DM total score of 7.28±4.33 and an average state anxiety scale value of 35.56+-9.21 and an average trait anxiety scale value of 34.04+-7.44 at the STAI-Y. A global severity index > 1 at SCL-90 was detected in 8.3% of the sample. Conclusions: The identification of antepartum distress and the early treatment of perinatal psychopathology represent a priority during the Covid-19 pandemic era

ANTEPARTUM DISTRESS DURING COVID-19 PANDEMIC: AN OBSERVATIONAL STUDY

Background: The present study investigates the impact of the Coronavirus diseases 2019 (Covid-19) pandemic on the subjective experience of pregnant women, as well as the impact of the pandemic on this population in terms of psychopathological correlates. Subjects and methods: Pregnant women referring to the Section of Obstetrics and Gynecology of the General Hospital of Perugia, Italy, were recruited from 1st May, 2021 to 15th June, 2021. Socio-demographic and clinical data was collected, as well as information regarding the Covid-19 pandemic impact on the subjective experience of pregnancy. Psychopathology was evaluated by means of the State-Trait Anxiety Inventory Form Y (STAI-Y), the Symptom Checklist-90 (SCL-90) and the Prenatal Distress Measure (Pre-DM). Descriptive analyses were performed. Significant associations between distress symptoms and the collected sociodemographic and clinical variables were assessed by using the Pearson correlation (p<0.05). Results: 25 women were included in the study. Among these, 18 (72%) reported that the Covid-19 pandemic negatively impacted their experience of pregnancy. Were detected an average Pre-DM total score of 7.28±4.33 and an average state anxiety scale value of 35.56+-9.21 and an average trait anxiety scale value of 34.04+-7.44 at the STAI-Y. A global severity index > 1 at SCL-90 was detected in 8.3% of the sample. Conclusions: The identification of antepartum distress and the early treatment of perinatal psychopathology represent a priority during the Covid-19 pandemic era

Whole Plant Temperature Manipulation Affects Flavonoid Metabolism and the Transcriptome of Grapevine Berries

Among environmental factors, temperature is the one that poses serious threats to viticulture in the present and future scenarios of global climate change. In this work, we evaluated the effects on berry ripening of two thermal regimes, imposed from veraison to harvest. Potted vines were grown in two air-conditioned greenhouses with High Temperature (HT) and Low Temperature (LT) regimes characterized by 26 and 21\ub0C as average and 42 and 35\ub0C as maximum air daily temperature, respectively. We conducted analyses of the main berry compositional parameters, berry skin flavonoids and berry skin transcriptome on HT and LT berries sampled during ripening. The two thermal conditions strongly differentiated the berries. HT regime increased sugar accumulation at the beginning of ripening, but not at harvest, when HT treatment contributed to a slight total acidity reduction and pH increase. Conversely, growing temperatures greatly impacted on anthocyanin and flavonol concentrations, which resulted as strongly reduced, while no effects were found on skin tannins accumulation. Berry transcriptome was analyzed with several approaches in order to identify genes with different expression profile in berries ripened under HT or LT conditions. The analysis of whole transcriptome showed that the main differences emerging from this approach appeared to be more due to a shift in the ripening process, rather than to a strong rearrangement at transcriptional level, revealing that the LT temperature regime could delay berry ripening, at least in the early stages. Moreover, the results of the in-depth screening of genes differentially expressed in HT and LT did not highlight differences in the expression of transcripts involved in the biosynthesis of flavonoids (with the exception of PAL and STS) despite the enzymatic activities of PALs and UFGT being significantly higher in LT than HT. This suggests only a partial correlation between molecular and biochemical data in our conditions and the putative existence of post-transcriptional and post-translational mechanisms playing significant roles in the regulation of flavonoid metabolic pathways and in particular of anthocyanins