Investments in oil field development by the example of Tomsk oblast

The article describes the geologic structure of the formation located not far from Strezhevoy Tomsk Oblast. The formation has been poorly studied by seismic methods. The reserves categories С[1] and С[2] as well as hydrocarbon potential are presented. 4 exploratory and 39 production wells are designed to be drilled depending on geologic knowledge and formation conditions. The article deals with the investment plan including development, oil export expenditures and implementing cost calculation

Synthesis and Research of Alumina Ceramics Properties

The article describes in detail alumina powder synthesis by different methods at varying parameters. The technique of obtaining ceramics and the research of the optical properties for determining the materials with the maximum luminescence efficiency is presented. The concentration of the luminescence intrinsic centers and various defects differ for ceramics synthesized by different methods. It is determined that ceramics based on the powder synthesized by a sol-gel method has the maximum thermoluminescence intensity in the F-center peak, whereas for the peak of 360 °C it is obtained with the powder prepared by precipitation of aluminum nitrate with a PEG‑20000 stabilizer

Variability in the relative quantity of human DNA resulted from metagenomic analysis of gut microbiota

We conducted the comparative study of seven different methods of total DNA extraction from human feces. All these methods are recommended in protocols for metagenomic analysis of human gut microbiota. We studied the relative quantity of human DNA calculated from shotgun sequencing on a SOLiD 4 genetic analyzer of metagenomic samples. It was shown that either initial amount of feces or a method applied for total DNA extraction do not affect on final relative human DNA abundance, which is less than 1% in healthy people. Invariance of this parameter allows to consider increased abundance of human DNA in metagenomic samples as a potential marker of inflammatory bowel diseases. | Abstract available from the publisher

Amperometric detection of caffeine and paracetamol on a dual screen printed electrode modified with mixed-valence ruthenium and cobalt oxides in flow-injection analysis

It is found that the mixed-valence ruthenium and cobalt oxides (CoOx-RuOx) composite electrodeposited on screen printed electrode exhibits catalytic activity during caffeine and paracetamol electrooxidation. These compounds are oxidized at different potentials which provides selective voltammetric determination at simultaneous presence. A method of amperometric determination of caffeine and paracetamol on a dual electrode modified with CoOx-RuOx composite in a flow injection analysis is developed. The linear relationship between an analytical signal and analyte concentration is found in the range from 5×10-7 mol/l to 5×10-3 mol/l. Using a modified dual screen printed electrode as an amperometric detector in flow injection analysis allows to determine up to 360 samples/hour. The developed method has been tested in the analysis of some pharmaceuticals

Interval approach to processing the noised thermophysical data

The paper deals with application of numerical methods to processing the experimental data on the thermophysical properties of several chemical substances and their compounds (cryolites, rare earth compounds, and alkali halides). The main aim of investigations is in estimating the parameters of dependencies between the heat of fusion and the melting temperature of these chemical substances. The data are corrupted by the measuring errors. Procession is implemented under conditions of uncertainty: there is no any information on probabilistic properties of the corrupting factors, samples of measurements are short, and only approximate functions are known that describes mentioned dependencies. Under such conditions, the standard statistical methods can be applied formally. To obtain guarantied results in parameters estimation, the interval analysis methods and procedures are used. © 2020 American Institute of Physics Inc.. All rights reserved.The work was supported by the RFBR grants, projects nos. 18-01-00410 and 18-03-00785 А

[Changing the intestinal microbiota of chickens in ontogenesis] Изменение микробиоты кишечника кур в онтогенезе

This paper presents the results of a molecular genetic analysis of the changes in the composition of the microbiota of the blind processes of the intestine of the hens of the industrial loam "Lohmann Brown" during ontogeny. According to the results of the analysis of taxonomic affiliation it is established that over 70% of the phylotypes belong to the three phylums - Firmicutes, Bacteroidetes and Proteobacteria, less represented were Actinobacteria, Tenericutes and Fusobacteria, and a significant number of unidentified bacteria was detected. During ontogenesis, birds exhibited marked changes in the ratio of the number of phylotypes and taxonomic groups of the intestinal microbiota. At the age of 20-40 weeks, the birds showed a significant increase in the representatives of the Clostridia class involved in the metabolism of carbohydrates, acid-utilizing bacteria of the order Negativicutes and bacteria with high antagonistic properties (Bifidobacteriales, Bacillus), as well as a significant decrease in the content of a number of opportunistic and pathogenic taxa - family Enterobacteriaceae, the order of Pseudomonadales, phylum Tenericutes. The greatest homogeneity of the bacterial community of the blind processes of the gastrointestinal tract in laying hens was revealed at the age of 20 weeks, which is confirmed by the estimation of biodiversity by means of ecological indices. В настоящем исследовании представлены результаты молекулярно-генетического анализа изменения состава микробиоты слепых отростков кишечника кур промышленного кросса «Ломанн Браун» в течение онтогенеза. По результатам анализа таксономической принадлежности установлено, что свыше 70% филотипов относится к трем филумам - Firmicutes, Bacteroidetes и Proteobacteria, менее представлены были Actinobacteria, Tenericutes и Fusobacteria, а также выявлено присутствие значительного количества неидентифицированных бактерий. В течение онтогенеза у птиц наблюдались заметные изменения соотношения количества филотипов и таксономических групп микробиоты кишечника. В возрасте 20-40 недель у птиц отмечено достоверное увеличение представителей класса Clostridia, участвующих в метаболизме углеводов, кислот-утилизирующих бактерий порядка Negativicutes и бактерий с высокими антагонистическими свойствами (Bifidobacteriales, Bacillus), а также достоверное снижение содержания ряда условно-патогенных и патогенных таксонов – семейства Enterobacteriaceae, порядка Pseudomonadales, филума Tenericutes. Наибольшая однородность бактериального сообщества слепых отростков ЖКТ у несушек выявлена в 20-недельном возрасте, что подтверждается оценкой биоразнообразия при помощи экологических индексов

Atomic Ordering in Cubic Bismuth Telluride Alloy Phases at High Pressure

Pressure-induced transitions from ordered intermetallic phases to substitutional alloys to semi-ordered phases were studied in a series of bismuth tellurides. Using angle-dispersive x-ray diffraction, the compounds Bi4Te5, BiTe, and Bi2Te were observed to form alloys with the disordered body-centered cubic (bcc) crystal structure upon compression to above 14--19 GPa at room temperature. The BiTe and Bi2Te alloys and the previously discovered high-pressure alloys of Bi2Te3 and Bi4Te3 were all found to show atomic ordering after gentle annealing at very moderate temperatures of ~100{\deg}C. Upon annealing, BiTe transforms from the bcc to the B2 (CsCl) crystal structure type, and the other phases adopt semi-disordered variants thereof, featuring substitutional disorder on one of the two crystallographic sites. The transition pressures and atomic volumes of the alloy phases show systematic variations across the Bi_mTe_n series including the end members Bi and Te. First-principles calculations were performed to characterize the electronic structure and chemical bonding properties of B2-type BiTe and to identify the driving forces of the ordering transition. The calculated Fermi surface of B2-type BiTe has an intricate structure and is predicted to undergo three topological changes between 20 and 60 GPa.Comment: 8 pages, 11 figures, accepted for publication in Phys. Rev.

Morphology and Mechanical Properties of Li-In Alloy as a Promising Anode Material in Fully Solid-State Lithium Batteries

In this work, the phase composition and morphology of Li-In alloys have been studied. The distribution map of the Young's modulus over the surface of an alloy with 18 at % lithium was obtained using the AFM method. The distribution map of indium in alloys containing 10 at% and 18 at% lithium was obt

Факторы риска развития тяжелой дыхательной недостаточности у новорожденных c гестационным возрастом 37 недель

The aim was to determine the risk factors for severe respiratory failure in newborns of 37-week gestational age.Methods. The study was designed as retrospective, controlled, non-randomized, and single-center. 640 patients corresponded the criteria of inclusion and exclusion. 7 (1.1%) children died. Depending on the gestational age (GA), patients were divided into two groups. The main group (n = 279) included newborns with GA of 37 weeks. The comparison group (n = 361) consisted of newborns with GA of 34‒36 weeks. Both groups of children received comparable critical care and had similar settings of initial artificial pulmonary ventilation. The software of BioStat was used for statistical processing. Results. Patients of the main group had initially higher pressure in the right ventricle, they were in need of noninvasive ventilator more often (χ2 = 4.23; p = 0.05), and pulmonary hemorrhage were also more frequent in them (χ2 = 9.608; p = 0.02). It was mostly newborns delivered by cesarean section who developed respiratory problems. A uterine scar in a pregnant woman made a significant risk factor for severe respiratory failure in patients of the main group (OR ‒ 1.99).Conclusion. Severe respiratory failure in newborns with gestational age at 37 weeks develops with intrauterine growth retardation, the main risk factor of which is the presence of a scar on the uterus. Цель: определение факторов риска развития тяжелой дыхательной недостаточности у новорожденных c гестационным возрастом (ГВ) 37 нед.Методы. Дизайн исследования – ретроспективное, контролируемое, нерандомизированное, одноцентровое. Критериям включения и исключения соответствовало 640 пациентов. Умерло 7 (1,1%) детей. Пациенты в зависимости от ГВ разделены на две группы. Основная (n = 279) ‒ новорожденные с ГВ 37 нед. Группа сравнения (n = 361) ‒ новорожденные с ГВ 34‒36 нед. У детей обеих групп были сопоставимая интенсивная терапия и параметры стартовой искусственной вентиляции легких (ИВЛ). Статистическую обработку проводили на основе статистической программы BioStat.Результаты. У пациентов основной группы имелось исходно более высокое давление в правом желудочке, чаще использовалась неинвазивная ИВЛ (χ2 = 4,23; p = 0,05), была более высокой частота легочного кровотечения (χ2 = 9,608; p = 0,02). Респираторные проблемы развивались в основном у новорожденных, извлеченных путем кесарева сечения. Значимым фактором риска развития тяжелой дыхательной недостаточности у пациентов основной группы явилось наличие рубца на матке у беременной (ОШ ‒ 1,99).Вывод. Тяжелая дыхательная недостаточность у новорожденных с ГВ 37 нед. развивается при задержке внутриутробного развития, основным фактором риска которой является наличие рубца на матке

Реслизумаб в лечении больных тяжелой бронхиальной астмой эозинофильного фенотипа

It is known that patients with severe asthma often fail to achieve disease control. Excessive airways eosinophilic inflammation is one of the key causes of severe uncontrolled asthma in this case. The occurrence of eosinophilic phenotype of inflammation is quite high in severe uncontrolled asthma. Thus, about 55% of patients have eosinophil level in induced sputum ≥ 3%. Eosinophilic phenotype of asthma is associated with greater severity of symptoms, presence of atopy, late onset of the disease, and lack of response to inhaled glucocorticosteroids. Numerous studies confirmed the relationship between elevated eosinophils in the airways and more frequent and severe asthma exacerbations, as well as reduced lung function, increased administration of steroids and other medications, and more frequent use of healthcare services. Severe eosinophilic asthma is characterized mainly by late onset of the disease, persistent eosinophilia in the airways and peripheral blood. It is associated with frequent exacerbations, chronic or intermittent need to the use of systemic corticosteroids to achieve better control of the disease, and unfavorable prognosis of the natural course. Predominantly eosinophilic type of airway inflammation is a characteristic manifestation of T2 endotype of asthma, that is implemented due to the domination of Th2-lymphocyte response (allergic asthma) and/or due to high activity of type 2 innate lymphoid cells (ILC2) involved in the development of both non-allergic and allergic asthma. Th2 and ILC2 cells increase IL-5 level, which plays an important role in the formation of uncontrolled eosinophilic inflammation in the airways in patients suffering from T2 endotype of severe asthma, by stimulating eosinophil precursor maturation in the bone marrow, mobilization of eosinophils and precursors from the bone marrow, accumulation of eosinophils in the blood, eosinophilic infiltration of lung tissue, and eosinophil migration in the area of inflammation. The novel medication reslizumab (Cinqair) is the first anti-IL-5 immunological biologic drug registered in Russia for the treatment of severe asthma with eosinophilic airway inflammation. As a humanized monoclonal antibody (IgG4k) with high affinity for IL-5, reslizumab specifically binds to IL-5 and inhibits its interaction with IL-5 receptor on the cell surface, thus disrupting the underlying pathophysiology of bronchial inflammation in asthma, including maturation and survival of eosinophils, inflammation and remodeling of the airways. Clinical effects of reslizumab are manifested as decreased asthma exacerbation rate, improved lung function, and disease control.Известно, что у больных тяжелой бронхиальной астмой (БА) часто не достигается контроль над заболеванием. При этом избыточное эозинофильное воспаление дыхательных путей является одной из ключевых причин тяжелого неконтролируемого течения БА. В структуре тяжелой неконтролируемой БА частота эозинофильного фенотипа воспаления достаточно высока. Так, около 55 % пациентов характеризуются уровнем эозинофилов в индуцированной мокроте ≥ 3 %. Эозинофильный фенотип БА ассоциируется с большей выраженностью симптомов, аллергией, нередко с поздним развитием заболевания и неполным ответом на лечение ингаляционными глюкокортикостероидами (иГКС). В многочисленных исследованиях подтверждена связь между повышенным уровнем эозинофилов в дыхательных путях и более частыми и тяжелыми обострениями БА, а также сниженной функцией легких, повышенным приемом ГКС и других лекарственных препаратов, более частым использованием услуг здравоохранения. Тяжелая эозинофильная БА характеризуется преимущественно поздним началом заболевания, персистирующей эозинофилией в дыхательных путях и периферической крови. Ей свойственны частые обострения, постоянная или эпизодическая зависимость от применения системных ГКС для достижения лучшего контроля над заболеванием, а также неблагоприятный прогноз естественного течения. Преимущественно эозинофильный тип воспаления дыхательных путей является характерным проявлением Т2-эндотипа БА, который реализуется за счет доминирования Th2-лимфоцитарного ответа (аллергическая БА) и / или высокой активности врожденных лимфоидных клеток 2-го типа – ILC2-клеток, участвующих в развитии как неаллергической, так и аллергической БА. Как Th2-, так и ILC2-клетки увеличивают уровни интерлейкина-5 (IL-5), который играет важную роль в формировании неконтролируемого эозинофильного воспаления в бронхолегочном регионе у больных, страдающих Т2-эндотипом тяжелой БА, стимулируя созревание предшественников эозинофилов в костном мозге, мобилизацию эозинофилов и предшественников из костного мозга, накопление эозинофилов в крови, эозинофильную инфильтрацию ткани легких и миграцию эозинофилов в очаг воспаления. Новый препарат Реслизумаб (Синкейро) является первым зарегистрированным в России анти-IL-5 иммунологическим биопрепаратом для лечения тяжелой БА с эозинофильным типом воспаления дыхательных путей. Как гуманизированное моноклональное антитело (IgG4k), высокоафинное к IL-5, Реслизумаб специфически связывается с ним и препятствует взаимодействию IL-5 с его рецептором на поверхности клеток, нарушая процесс, лежащий в основе патофизиологии бронхиального воспаления при БА, включая созревание и выживаемость эозинофилов, воспаление и ремоделирование дыхательных путей. Клинические эффекты Реслизумаба проявляются снижением частоты обострений БА, улучшением функции легких и контроля над заболеванием