67 research outputs found

    Development of a global ~90m water body map using multi-temporal Landsat images

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    This paper describes the development of a Global 3 arc-second Water Body Map (G3WBM), using an automated algorithm to process multi-temporal Landsat images from the Global Land Survey (GLS) database. We used 33,890 scenes from 4 GLS epochs in order to delineate a seamless water body map, without cloud and ice/snow gaps. Permanent water bodies were distinguished from temporal water-covered areas by calculating the frequency of water body existence from overlapping, multi-temporal, Landsat scenes. By analyzing the frequency of water body existence at 3 arc-second resolution, the G3WBM separates river channels and floodplains more clearly than previous studies. This suggests that the use of multi-temporal images is as important as analysis at a higher resolution for global water body mapping. The global totals of delineated permanent water body area and temporal water-covered area are 3.25 and 0.49 million km2 respectively, which highlights the importance of river-floodplain separation using multi-temporal images. The accuracy of the water body classification was validated in Hokkaido (Japan) and in the contiguous United States using an existing water body databases. There was almost no commission error, and about 70% of lakes > 1 km2 shows relative water area error < 25%. Though smaller water bodies (< 1 km2) were underestimated mainly due to omission of shoreline pixels, the overall accuracy of the G3WBM should be adequate for larger scale research in hydrology, biogeochemistry, and climate systems and importantly includes a quantification of the temporal nature of global water bodies

    Central odontogenic fibroma: a case report with long-term follow-up

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    An osteolytic tumour of the mandible with prominent expansive growth on the alveolar ridge and displacement of the involved teeth is described in a 28-year-old man. The lesion was diagnosed as a central odontogenic fibroma, an uncommon benign neoplasm derived from dental apparatus, and was removed by curettage. The patient remains asymptomatic after thirteen years of follow-up, which supports the claimed indolent behavior of this poorly documented disease and the adequacy of a conservative surgical treatment

    Drosophila neuroblasts retain the daughter centrosome

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    During asymmetric mitosis, both in male Drosophila germline stem cells and in mouse embryo neural progenitors, the mother centrosome is retained by the self-renewed cell; hence suggesting that mother centrosome inheritance might contribute to stemness. We test this hypothesis in Drosophila neuroblasts (NBs) tracing photo converted centrioles and a daughter-centriole-specific marker generated by cloning the Drosophila homologue of human Centrobin. Here we show that upon asymmetric mitosis, the mother centrosome is inherited by the differentiating daughter cell. Our results demonstrate maturation-dependent centrosome fate in Drosophila NBs and that the stemness properties of these cells are not linked to mother centrosome inheritance

    G-protein signaling: back to the future

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    Heterotrimeric G-proteins are intracellular partners of G-protein-coupled receptors (GPCRs). GPCRs act on inactive Gα·GDP/Gβγ heterotrimers to promote GDP release and GTP binding, resulting in liberation of Gα from Gβγ. Gα·GTP and Gβγ target effectors including adenylyl cyclases, phospholipases and ion channels. Signaling is terminated by intrinsic GTPase activity of Gα and heterotrimer reformation — a cycle accelerated by ‘regulators of G-protein signaling’ (RGS proteins). Recent studies have identified several unconventional G-protein signaling pathways that diverge from this standard model. Whereas phospholipase C (PLC) β is activated by Gαq and Gβγ, novel PLC isoforms are regulated by both heterotrimeric and Ras-superfamily G-proteins. An Arabidopsis protein has been discovered containing both GPCR and RGS domains within the same protein. Most surprisingly, a receptor-independent Gα nucleotide cycle that regulates cell division has been delineated in both Caenorhabditis elegans and Drosophila melanogaster. Here, we revisit classical heterotrimeric G-protein signaling and explore these new, non-canonical G-protein signaling pathways

    Parameters in panoramic radiography for differentiation of radiolucent lesions

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    OBJECTIVE: The aims of this study were to establish parameters in panoramic radiography for interpretation of unilocular radiolucent lesions, and to compare the accuracy of diagnoses given by examiners before and after using these parameters. MATERIAL AND METHODS: In Part I, 12 specialists analyzed 24 images and the diagnostic criteria used by each examiner to make correct diagnoses were used to build a list of basic radiographic parameters for each pathology (ameloblastoma, keratocystic odontogenic tumor, dentigerous cyst, and idiopathic bone cavity). In Part II, this list was used by 6 undergraduate students (Un), 8 recently graduated dentists (D), 3 oral pathologists, 3 stomatologists, 3 oral radiologists, and 3 oral surgeons to diagnose the corresponding pathologies in the other set of 24 panoramic radiographs (T2). The same analysis occurred without using this list (T1). The method of generalized estimating equations (GEE) was used in order to estimate the probability of making a correct diagnosis depending on the specialty of the examiner, type of lesion, and moment of the evaluation, T1 or T2 (before or after they had access to the list of parameters, respectively). RESULTS: Higher values were obtained for the probability (GEE) of making a correct diagnosis on T2; the group Un presented the highest improvement (14.6 %); no differences between the probabilities were observed either between Un and D, or among the different groups of specialists. CONCLUSIONS: The use of panoramic radiographic parameters did allow improving the diagnostic accuracy for all groups of examiners
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