Detecção do complexo Mycobacterium tuberculosis por nested polymerase chain reaction em espécimes pulmonares e extrapulmonares

Submitted by Kamylla Nascimento ([email protected]) on 2017-12-07T12:48:00Z No. of bitstreams: 2 art. Detection of Mycobacterium - furini.pdf: 173341 bytes, checksum: 86c36f0c01dedf73d85219a942bfb783 (MD5) art. Detection of Mycobacterium - furini port..pdf: 183111 bytes, checksum: 258c0179c4efe0f597ec9b3eb2292291 (MD5)Approved for entry into archive by Kamylla Nascimento ([email protected]) on 2017-12-07T13:07:17Z (GMT) No. of bitstreams: 2 art. Detection of Mycobacterium - furini.pdf: 173341 bytes, checksum: 86c36f0c01dedf73d85219a942bfb783 (MD5) art. Detection of Mycobacterium - furini port..pdf: 183111 bytes, checksum: 258c0179c4efe0f597ec9b3eb2292291 (MD5)Made available in DSpace on 2017-12-07T13:07:17Z (GMT). No. of bitstreams: 2 art. Detection of Mycobacterium - furini.pdf: 173341 bytes, checksum: 86c36f0c01dedf73d85219a942bfb783 (MD5) art. Detection of Mycobacterium - furini port..pdf: 183111 bytes, checksum: 258c0179c4efe0f597ec9b3eb2292291 (MD5) Previous issue date: 2013São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.Adolfo Lutz Institute. Department of Mycobacteria. São José do Rio Preto, SP, Brazil.Rio Preto University Center. São José do Rio Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Evandro Chagas Institute. Belém, PA, Brazil.Regional Foundation School of Medicine. São José do Rio Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Lauro de Souza Lima Institute. Bauru, SP, Brazil.Fluminense Federal University. Niterói, RJ, Brazil.Comparar o desempenho da técnica nested polymerase chain reaction (NPCR) com aquele de culturas na detecção do complexo Mycobacterium tuberculosis em espécimes pulmonares e extrapulmonares.To compare the performance of nested polymerase chain reaction (NPCR) with that of cultures in the detection of the Mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens

Leprosy survey among rural communities and wild armadillos from Amazonas state, Northern Brazil.

There is evidence that in southern US, leprosy is a zoonosis infecting wild Dasypus novemcinctus armadillos but the extent of this finding is unknown. This ecological study investigated leprosy in rural communities and in wild armadillos from the Brazilian Amazon. The study area was the Mamiá Lake of Coari municipality, Amazonas State, Northern region, a hyper endemic leprosy area where residents live on subsistence farming, fishing and armadillo hunting and its meat intake are frequent. The leprosy survey was conducted in sixteen communities by a visiting team of specialists. Local partakers provided wild armadillos to investigate M. leprae infection. Volunteers had complete dermato-neurological examination by a dermatologist with expertise in leprosy diagnosis, suspect skin lesions were biopsied for histopathology (Hematoxylin-eosin/HE, Fite-Faraco/FF staining); slit skin smears were collected. Armadillos' tissue fragments (skins, spleens, livers, lymph nodes, adrenal glands, others) were prepared for histopathology (HE/FF) and for M. leprae repetitive element-RLEP-qPCR. Among 176 volunteers, six new indeterminate leprosy cases were identified (incidence = 3.4%). Suspect skin sections and slit skin smears were negative for bacilli. Twelve wild D. novemcinctus were investigated (48 specimens/96 slides) and histopathological features of M. leprae infection were not found, except for one skin presenting unspecific inflammatory infiltrate suggestive of indeterminate leprosy. Possible traumatic neuroma, granuloma with epithelioid and Langhans cells, foreign-body granuloma were also identified. Granulomatous/non-granulomatous dermatitides were periodic-acid-Schiff/PAS negative for fungus. M. leprae-RLEP-qPCR was negative in all armadillos' tissues; no bacillus was found in histopathology. Our survey in rural communities confirmed the high endemicity for leprosy while one armadillo was compatible with paucibacillary M. leprae infection. At least in the highly endemic rural area of Coari, in the Brazilian Amazon region where infectious sources from untreated multibacillary leprosy are abundant, M. leprae infected armadillos may not represent a major source of infection nor a significant public health concern

Toll-like receptor 1 N248S single-nucleotide polymorphism is associated with leprosy risk and regulates immune activation during mycobacterial infection

Conflicting findings about the association between leprosy and TLR1 variants N248S and I602S have been reported. Here, we performed case-control and family based studies, followed by replication in 2 case-control populations from Brazil, involving 3162 individuals. Results indicated an association between TLR1 248S and leprosy in the case-control study (SS genotype odds ratio [OR], 1.81; P = .004) and the family based study (z = 2.02; P = .05). This association was consistently replicated in other populations (combined OR, 1.51; P < .001), corroborating the finding that 248S is a susceptibility factor for leprosy. Additionally, we demonstrated that peripheral blood mononuclear cells (PBMCs) carrying 248S produce a lower tumor necrosis factor/interleukin-10 ratio when stimulated with Mycobacterium leprae but not with lipopolysaccharide or PAM3cysK4. The same effect was observed after infection of PBMCs with the Moreau strain of bacillus Calmette-Guerin but not after infection with other strains. Finally, molecular dynamics simulations indicated that the Toll-like receptor 1 structure containing 248S amino acid is different from the structure containing 248N. Our results suggest that TLR1 248S is associated with an increased risk for leprosy, consistent with its hypoimmune regulatory function.Fundação Oswaldo Cruz/Programa Estratégico de Apoio à Pesquisa em SaúdeFundação Oswaldo Cruz/Programa Estratégico de Apoio à Pesquisa em SaúdeInstituto Oswaldo Cruz/FIOCRUZ (internal funds)Instituto Oswaldo Cruz/FIOCRUZ (internal funds)Fundação de Amparo a Pesquisa do Estado do Rio de JaneiroFundacao do Amparo a Pesquisa do Estado do Rio de JaneiroFundacao de Amparo a Pesquisa do Estado de Sao PauloFundação de Amparo a Pesquisa do Estado de São PauloConselho Nacional de Pesquisa e Desenvolvimento TecnológicoConselho Nacional de Pesquisa e Desenvolvimento Tecnológic

Toll-like receptor 1 (TLR1) N248S single nucleotide polymorphism is associated with leprosy risk and regulates immune activation during mycobacterial infection

Submitted by sandra infurna ([email protected]) on 2016-01-21T12:52:44Z No. of bitstreams: 1 carolinnes_marques_etal_IOC_2013.pdf: 681352 bytes, checksum: 93595bbcfadc6e1fc928835a67a3c3e2 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-01-21T13:14:09Z (GMT) No. of bitstreams: 1 carolinnes_marques_etal_IOC_2013.pdf: 681352 bytes, checksum: 93595bbcfadc6e1fc928835a67a3c3e2 (MD5)Made available in DSpace on 2016-01-21T13:14:09Z (GMT). No. of bitstreams: 1 carolinnes_marques_etal_IOC_2013.pdf: 681352 bytes, checksum: 93595bbcfadc6e1fc928835a67a3c3e2 (MD5) Previous issue date: 2013Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica (PROCC). Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Departamento de Enfermagem Materno-Infantil e Saúde Pública. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Universidade de São Paulo. Escola de Odontologia de Bauru. Departamento de Ciências Biológicas. Bauru, SP, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Católica do Paraná. Escola de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Curitiba, PR, Brasil.Universidade Federal de Minas Gerais. Departamento de Enfermagem Materno-Infantil e Saúde Pública. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica (PROCC). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica (PROCC). Rio de Janeiro, RJ, Brasil.Instituto Lauro de Souza Lima. Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.TLR1 variants N248S and I602S have been described associated with leprosy, but different results were found. Here, we performed both a case-control and family-based studies followed by replication in two case-control samples from Brazil, enrolling 3,162 individuals. Results indicated risk association between TLR1 248S and leprosy in case-control (OR SS genotype= 1.81, p=0.004) and familial study (z=2.02, p=0.05). This association was consistently replicated in other populations (ORCombined=1.51, p-value<0.001), corroborating 248S as a susceptibility factor for leprosy. Additionally, we demonstrated that PBMCs carrying 248S produce lower log(TNF/IL-10) when stimulated with M. leprae, but not with LPS or PAM3cysK4. The same effect was observed after infection of PBMCs with BCG Moreau, but not other strains. Finally, molecular dynamics simulations indicated that 248S-TLR1 structure is different than 248N-TLR1. Our results suggest that TLR1 248S is associated with leprosy risk, consistent with its hypo-immune regulatory function