Substructures in the core of Abell 2319

We analysed the deep archival Chandra observations of the high-temperature galaxy cluster Abell 2319 to investigate the prominent cold front in its core. The main sharp arc of the front shows wiggles, or variations of the radius of the density jump along the arc. At the southern end of the arc is a feature that resembles a Kelvin-Helmholtz (KH) eddy, beyond which the sharp front dissolves. These features suggest that KH instabilities develop at the front. Under this assumption, we can place an upper limit on the ICM viscosity that is several times below the isotropic Spitzer value. Other features include a split of the cold front at its northern edge, which may be another KH eddy. There is a small pocket of hot, less-dense gas inside the cold front, which may indicate a `hole' in the front's magnetic insulation layer that lets the heat from the outer gas to penetrate inside the front. Finally, a large concave brightness feature southwest of the cluster core can be caused by the gasdynamic instabilities. We speculate that it can also be an inner boundary of a giant AGN bubble, similar to that in Ophiuchus. If the latter interpretation is supported by better radio data, this could be a remnant of another extremely powerful AGN outburst.Comment: Accepted for publication in MNRA

Pretransplant serum ferritin and C-reactive protein as predictive factors for early bacterial infection after allogeneic hematopoietic cell transplantation.

Although fluoroquinolones or other antibiotics are commonly used to prevent bacterial infections after hematopoietic cell transplantation (HCT), because of the growing presence of multidrug-resistant microorganisms, it is important to identify patients who are more likely to benefit from antibacterial prophylaxis. To evaluate risk factors for early bacterial infection after allogeneic HCT, we retrospectively analyzed clinical data for 112 consecutive adult patients with hematological malignancies who received transplants without any antibacterial prophylaxis. The cumulative incidence of bacterial infection at 30 days after transplantation was 16%. Among various pre-transplant factors, only high serum ferritin (>700 ng/mL, 47 patients) and high C-reactive protein (CRP) (>0.3 mg/dL, 28 patients) levels were significantly associated with the development of bacterial infection in a multivariate analysis (hazard ratio (95% confidence interval): ferritin, 4.00 (1.32-12.17); CRP, 3.64 (1.44-9.20)). In addition, septic shock and sepsis with organ failure were exclusively observed in patients who had high ferritin and/or high CRP levels. These results suggest that pretransplant serum ferritin and CRP levels can be useful markers for predicting the risk of early bacterial infection after allogeneic HCT. It may be prudent to limit antibacterial prophylaxis to patients with predefined risk factors to ensure the safety of HCT with the use of fewer antibiotics

Self-renewal capability of hepatocytic parental progenitor cells derived from adult rat liver is maintained long term when cultured on laminin 111 in serum-free medium

ラット小型肝細胞の前駆細胞は，Laminin(LN)111上で自己複製能と肝細胞としての基本機能を維持しながら継代培養可能であることがわかった．また，継代培養した前駆細胞はMatrigel積層培養で成熟化する

CAMELOT: Design and performance verification of the detector concept and localization capability

A fleet of nanosatellites using precise timing synchronization provided by the Global Positioning System is a new concept for monitoring the gamma-ray sky that can achieve both all-sky coverage and good localization accuracy. We are proposing this new concept for the mission CubeSats Applied for MEasuring and LOcalising Transients (CAMELOT). The differences in photon arrival times at each satellite are to be used for source localization. Detectors with good photon statistics and the development of a localization algorithm capable of handling a large number of satellites are both essential for this mission. Large, thin CsI scintillator plates are the current candidates for the detectors because of their high light yields. It is challenging to maximize the light-collection efficiency and to understand the position dependence of such thin plates. We have found a multi-channel readout that uses the coincidence technique to be very effective in increasing the light output while keeping a similar noise level to that of a single channel readout. Based on such a detector design, we have developed a localization algorithm for this mission and have found that we can achieve a localization accuracy better than 20 arc minutes and a rate of about 10 short gamma-ray bursts per year

Human annexin A6 interacts with influenza a virus protein M2 and negatively modulates infection

Copyright © 2012, American Society for Microbiology. All Rights ReservedThe influenza A virus M2 ion channel protein has the longest cytoplasmic tail (CT) among the three viral envelope proteins and is well conserved between different viral strains. It is accessible to the host cellular machinery after fusion with the endosomal membrane and during the trafficking, assembly, and budding processes. We hypothesized that identification of host cellular interactants of M2 CT could help us to better understand the molecular mechanisms regulating the M2-dependent stages of the virus life cycle. Using yeast two-hybrid screening with M2 CT as bait, a novel interaction with the human annexin A6 (AnxA6) protein was identified, and their physical interaction was confirmed by coimmunoprecipitation assay and a colocalization study of virus-infected human cells. We found that small interfering RNA (siRNA)-mediated knockdown of AnxA6 expression significantly increased virus production, while its overexpression could reduce the titer of virus progeny, suggesting a negative regulatory role for AnxA6 during influenza A virus infection. Further characterization revealed that AnxA6 depletion or overexpression had no effect on the early stages of the virus life cycle or on viral RNA replication but impaired the release of progeny virus, as suggested by delayed or defective budding events observed at the plasma membrane of virus-infected cells by transmission electron microscopy. Collectively, this work identifies AnxA6 as a novel cellular regulator that targets and impairs the virus budding and release stages of the influenza A virus life cycle.This work was supported by the Research Fund for the Control of Infectious Disease (project 09080892) of the Hong Kong Government, the Area of Excellence Scheme of the University Grants Committee (grant AoE/M-12/-06 of the Hong Kong Special Administrative Region, China), the French Ministry of Health, the RESPARI Pasteur Network

CAMELOT: Cubesats Applied for MEasuring and LOcalising Transients mission overview

We propose a fleet of nanosatellites to perform an all-sky monitoring and timing based localisation of gamma-ray transients. The fleet of at least nine 3U cubesats shall be equipped with large and thin CsI(Tl) scintillator based soft gamma-ray detectors read out by multi-pixel photon counters. For bright short gamma-ray bursts (GRBs), by cross-correlating their light curves, the fleet shall be able to determine the time difference of the arriving GRB signal between the satellites and thus determine the source position with an accuracy of similar to 10'. This requirement demands precise time synchronization and accurate time stamping of the detected gamma-ray photons, which will be achieved by using on-board GPS receivers. Rapid follow up observations at other wavelengths require the capability for fast, nearly simultaneous downlink of data using a global inter-satellite communication network. In terms of all-sky coverage, the proposed fleet will outperform all GRB monitoring missions