Uji Aktivitas Ekstrak Kencur terhadap Pengendalian Pertumbuhan Fusarium Oxysporum dan Implementasinya dalam Pembuatan Flipbook

This research aims to determine the activity of kencur extract against Fusarium oxysporum control fungal growth and the effective concentration and determine the validity flipbook as a medium of learning materials. The research consisted of two phases. First, test the activity of the kencur extract on the growth of Fusarium oxysporum using experimental methods with 10 treatments and 3 replications were analyzed by one-way ANOVA test. Second, to test the validity of flipbook by 7 validator validated and analyzed based on the amount of the average score of all aspects. The results of the research kencur extract has the ability to inhibit the growth of Fusarium oxysporum is characterized by increasingly shrinking diameter fungi and effective concentration of 0,5%. The results of the validation analysis flipbook media obtained with an average score of 3,7 and classified as invalid so it fit for use in the learning process at the sub material environmental pollution in first grade of senior high school

Efek Aplikasi Topikal Gel Ekstrak Pandan Wangi terhadap Penyembuhan Luka Gingiva

The Effect of topical application pandan extract gel on gingival wound. Post-gingivectomy wound is usually covered by periodontal dressing, which generally contains some chemical compounds to protect the wound. However, it can provide allergic effect on some patients. Pandan leaves (Pandanus amaryllifolius Roxb.) contain a number of active substances that have anti-inflammatory, antioxidant and antibacterial effect and play a role in wound healing. This study aims to determine the effect of topical application of Pandan leaf extract gel on gingival wound healing. Gingivectomy model was carried out on mandibular incisive gingival using the 2.5-mm punch biopsy. Thirty-six rats were randomly divided into 3 groups of treatment: negative control (CMC-Na), positive control (Aloclair), and 50% of pandan extract gel. The gels on each group were applied (twice in a day) to the wound area after gingivectomy. The observation of the wound healing process was also carried out on day 1, 3, 7, and 14 by making the histological preparations of gingival wound area. The number of blood vessels was observed using microscope and data was analysed using Two- Way Anova and LSD. The result showed that number of blood vessel increased on day 3 and the peak was on day 7. Anova and LSD test showed several significant differences comparison the number blood vessel between treatment and control. In conclusion, topical application Pandan leaves extract gel could accelerate gingival wound healing

Looking to the future of zebrafish as a model to understand the genetic basis of eye disease

In this brief commentary, we provide some of our thoughts and opinions on the current and future use of zebrafish to model human eye disease, dissect pathological progression and advance in our understanding of the genetic bases of microphthalmia, andophthalmia and coloboma (MAC) in humans. We provide some background on eye formation in fish and conservation and divergence across vertebrates in this process, discuss different approaches for manipulating gene function and speculate on future research areas where we think research using fish may prove to be particularly effective

Pengaturan Hak Pekerja Perempuan di Indonesia dalam Perspektif Convention On The Elimination Of All Forms Discrimination Againts Women (CEDAW)

Penelitian ini mengkaji tentang pengaturan hak pekerja perempuanyang diatur dalam Convention On The Elimination Of All Forms Discrimination Againts Women (CEDAW) dan Undang- Undang  Nomor 13 Tahun 2003 tentang Ketenagakerjaan beserta peraturan Perundang-undangan pelaksananya. Dikarenakan pekerja perempuan rentan akan kasus diskrimanasi di tempat kerja Rumusan masalah penelitian ini adalah bagaimana kesesuaian antara Convention On The Elimination Of All Forms Discrimination Againts Women (CEDAW) dengan Undang- Undang  Nomor 13 Tahun 2003 tentang Ketenagakerjaan dalam mengatur hak pekerja perempuan, serta apa urgensi penyesuaiannya diantara keduanaya.Tujuan penelitian ini adalah untuk mengetahui sejauh mana kesesuaian antara Convention On The Elimination Of All Forms Discrimination Againts Women (CEDAW) dengan Undang- Undang  Nomor 13 Tahun 2003 tentang Ketenagakerjaan dalam mengatur hak pekerja perempuan, serta untuk mengetahui urgensi penyesuaian antara keduanya. Metode penelitian yang digunakan dalam penelitian ini yaitu yuridis normatif, yang menggunakan pendekatan Perundang-undangan dan pendekatan kasus yang telah berkekuatan hukum tetap. Adapun hasil penelitian ini bahwa Dari segi penggolongan hak pekerja perempuan dinilai Undang-Undang Nomor 13 Tahun 2003 tentang Ketenagakerjaan telah sesuai dengan Convention on the Elimination of All Forms Discrimination Againts Women (CEDAW) dalam mengatur hak pekerja perempuan di Indonesia

Penyuluhan dan Pelatihan Kader Posyandu sebagai Upaya Peningkatan Wawasan Pelayanan Gizi Bagi Masyarakat

Peran aktif kader kesehatan dipelayanan gizi sangat penting untuk meningkatkan kualitas dan status gizi masyarakat terutama kesehatan ibu dan anak. Aktif tidaknya kader tersebut tergantung dari pengetahuan kader Posyandu. Kegiatan ini bertujuan untuk melakukan penyuluhan dan pelatihan kader posyandu agar lebih meningkatkan wawasan tentang pelayanan gizi di posyandu. Kegiatan pengabdian dilakukan melalui beberapa tahap yaitu tahap persiapan (observasi), tahap pelaksanaan, dan tahap evaluasi. Tahap awal dilakukan pengumpulan data melalui wawancara dan kuesioner (pre-test). Tahap inti dilakukan penyuluhan tentang 5 meja posyandu, MP ASI, PMT penyuluhan dan pemulihan, GTM, kemudian pelatihan pengisian KMS dan cara membaca grafik KMS, serta cara melakukan pengukuran status gizi yang baik dan benar. Tahap akhir dilakukan evaluasi melalui tanya jawab dan post-test untuk mengetahui tingkat pemahaman kader posyandu setelah penyuluhan. Hasil pre-test rata-rata pengetahuan kader sebesar 57.8 % dan post-test sebesar 73.3%. Hasil tersebut dapat disimpulkan bahwa pengetahuan kader meningkat setelah diberikan penyuluhan dan pelatihan

Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells.

Apical radial glia (aRG), the stem cells in developing neocortex, are unique bipolar epithelial cells, extending an apical process to the ventricle and a basal process to the basal lamina. Here, we report novel features of the Golgi apparatus, a central organelle for cell polarity, in mouse aRGs. The Golgi was confined to the apical process but not associated with apical centrosome(s). In contrast, in aRG-derived, delaminating basal progenitors that lose apical polarity, the Golgi became pericentrosomal. The aRG Golgi underwent evolutionarily conserved, accordion-like compression and extension concomitant with cell cycle-dependent nuclear migration. Importantly, in line with endoplasmic reticulum but not Golgi being present in the aRG basal process, its plasma membrane contained glycans lacking Golgi processing, consistent with direct ER-to-cell surface membrane traffic. Our study reveals hitherto unknown complexity of neural stem cell polarity, differential Golgi contribution to their specific architecture, and fundamental Golgi re-organization upon cell fate change

Antimalarial Properties of Isoquinoline Derivative from Streptomyces hygroscopicus subsp. Hygroscopicus: An In Silico Approach

Malaria is one of the life-threatening diseases in the world. The spread of resistance to antimalarial drugs is a major challenge, and resistance to artemisinin has been reported in the Southeast Asian region. In the previous study, the active compound of Streptomyces hygroscopicus subsp. Hygroscopicus (S. hygroscopicus), eponemycin, has been shown to have antimalarial effects. To further analyze the effects of other active compounds on the Plasmodium parasite, identifying and analyzing the effectiveness of compounds contained in S. hygroscopicus through instrumentation of liquid chromatography/mass spectrometry (LC/MS) and in silico studies were very useful. This study aimed at identifying other derivative compounds from S. hygroscopicus and screening the antimalarial activity of the compound by assessing the binding affinity, pharmacokinetic profile, and bond interaction. The derivative compounds were identified using LC/MS. Protein targets for derivative compounds were found through literature studies, and the results of identification of compounds and protein targets were reconstructed into three-dimensional models. Prediction of pharmacokinetic profiles was carried out using Swiss ADME. Screening of protein targets for the derivative compound was carried out using the reverse molecular docking method. Analyzing bond interaction was done by LigPlot. One compound from S. hygroscopicus, i.e., 6,7-dinitro-2-[1, 2, 4]triazole-4-yl-benzo[de]isoquinoline-1,3-dione, was successfully identified using LC/MS. This compound was an isoquinoline derivative compound. Through literature studies with inclusion criteria, thirteen protein targets were obtained for reverse molecular docking. This isoquinoline derivative had the potential to bind to each protein target. The pharmacokinetic profile showed that this compound had the drug-likeness criteria. Conclusion. 6,7-Dinitro-2-[1, 2, 4]triazole-4-yl-benzo[de]isoquinoline-1,3-dione has antimalarial activity as shown by reverse molecular docking studies and pharmacokinetic profiles. The best inhibitory ability of compounds based on bond affinity is with adenylosuccinate synthetase

The duration of response to intra-articular steroid injections in patients with osteoarthritis of the knee: a single centre's experience

Background: The burden of Osteoarthritis (OA) is huge, with a sizable proportion of patients that have failed the analgesic treatment and are not candidates for surgery or have refused surgery. Intraarticular corticosteroid injections (IASI) are considered standard of care for pain relief and control of local inflammation in this category of OA patients. However, there is a wide variation in the duration of response to steroid injections. This study was designed to determine the duration of response to IASI in patients with osteoarthritis of the knee in our environment. Methods: Fifty-four patients aged between 30 and 80 years who have been diagnosed with osteoarthritis using the American College of Rheumatology criteria and have met the inclusion criteria were enrolled in the study and were given intra-articular steroid injections to the knee. Their responses were assessed using visual analogue scale (VAS) score, Western Ontario, and McMaster Universities Osteoarthritis Index (WOMAC) score at 2, 4 and 12 weeks. The patients were classified as responders if there was a fifty percent improvement in the WOMAC scores and a fifty percent reduction in the VAS score and non-responders if there is less than fifty percent improvement in the WOMAC score and less than fifty percent reduction in the VAS score. Results: Fifty-two patients completed the study; 78.4% and 100% of these had good WOMAC and VAS responses respectively at 2 weeks but this proportion gradually reduced to 47.9% and 56.3% for WOMAC and VAS respectively at 3 months. Conclusion: Intra-articular steroid injections provide sustained response in patients with osteoarthritis of the knee who have failed analgesic therapy and are not candidates for total knee replacement for up to three months and the response decreases with advancing age