Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout Oncorhynchus mykiss spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?

Background: In rainbow trout (Oncorhynchus mykiss), the endocrine control of spermiation is not fully understood. Besides IIketotestosterone (IIKT) and 17alpha, 20beta-dihydroxyprogesterone (MIS), the potential physiological ligand of the mineralocorticoid receptor (MR) II-deoxycorticosterone (DOC), is a credible candidate in O. mykiss spermiation regulation as spermiation is accompanied with changes in aqueous and ionic flows. Methods: In this study, we investigated potential roles of DOC during spermiation 1) by describing changes in blood plasma DOC level, MR mRNA abundance during the reproductive cycle and MR localization in the reproductive tract 2) by investigating and comparing the effects of DOC (10 mg/kg) and MIS (5 mg/kg) supplementations on sperm parameters 3) by measuring the in vitro effect of DOC on testis MIS production. Results: The plasma concentration of DOC increased rapidly at the end of the reproductive cycle to reach levels that were 10-50 fold higher in mature males than in immature fish. MR mRNA relative abundance was lower in maturing testes when compared to immature testes, but increased rapidly during the spermiation period, immediately after the plasma rise in DOC. At this stage, immunohistochemistry localized MR protein to cells situated at the periphery of the seminiferous tubules and in the efferent ducts. Neither DOC nor MIS had significant effects on the mean sperm volume, although MIS treatment significantly increased the percentage of males producing milt. However, a significant reduction in the spermatocrit was observed when DOC and MIS were administrated together. Finally, we detected an inhibitory effect of DOC on testis MIS production in vitro. Conclusion: These results are in agreement with potential roles of DOC and MR during spermiation and support the hypothesis that DOC and MIS mechanisms of action are linked during this reproductive stage, maybe controlling milt fluidity. They also confirm that in O. mykiss MIS is involved in spermiation induction

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Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?-3

T different times post treatment. Means+ SD (n= 48–66). (B) Daily decomposition of this treatment effect. Means+ SD (n = 6–18). Different letters indicate significant differences between treatments, p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?"</p><p>http://www.rbej.com/content/6/1/19</p><p>Reproductive biology and endocrinology : RB&E 2008;6():19-19.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2430966.</p><p></p

Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?-0

(pg/ml) (B) and in testis mineralocorticoid receptor (rtMR) mRNA abundance (C) according to time of year and stage of sexual maturation. Means+ SD (n = 3–5). Mature males were only studied until December. with from a spring spawning strain. Kinetics of spermiation in a group of mature males, assessed by the spermiation index (D). Coincident changes in blood plasma DOC concentrations (pg/ml) (E) and in blood plasma MIS (ng/ml) (F) according to time of year. Means+ SD (n = 8). Different letters indicate significant differences between months. * Significant difference between immature and maturing/mature fish, p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?"</p><p>http://www.rbej.com/content/6/1/19</p><p>Reproductive biology and endocrinology : RB&E 2008;6():19-19.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2430966.</p><p></p

Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?-5

(pg/ml) (B) and in testis mineralocorticoid receptor (rtMR) mRNA abundance (C) according to time of year and stage of sexual maturation. Means+ SD (n = 3–5). Mature males were only studied until December. with from a spring spawning strain. Kinetics of spermiation in a group of mature males, assessed by the spermiation index (D). Coincident changes in blood plasma DOC concentrations (pg/ml) (E) and in blood plasma MIS (ng/ml) (F) according to time of year. Means+ SD (n = 8). Different letters indicate significant differences between months. * Significant difference between immature and maturing/mature fish, p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?"</p><p>http://www.rbej.com/content/6/1/19</p><p>Reproductive biology and endocrinology : RB&E 2008;6():19-19.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2430966.</p><p></p

Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?-1

III; F: vas deferens) followed by secondary antibody incubations and microscopy. Magnification: 400×.<p><b>Copyright information:</b></p><p>Taken from "Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?"</p><p>http://www.rbej.com/content/6/1/19</p><p>Reproductive biology and endocrinology : RB&E 2008;6():19-19.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2430966.</p><p></p

Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?-2

Ndicate significant differences between treatments, p < 0.05.<p><b>Copyright information:</b></p><p>Taken from "Plasma 11-deoxycorticosterone (DOC) and mineralocorticoid receptor testicular expression during rainbow trout spermiation: implication with 17alpha, 20beta-dihydroxyprogesterone on the milt fluidity?"</p><p>http://www.rbej.com/content/6/1/19</p><p>Reproductive biology and endocrinology : RB&E 2008;6():19-19.</p><p>Published online 19 May 2008</p><p>PMCID:PMC2430966.</p><p></p