HIV Antigen Incorporation within Adenovirus Hexon Hypervariable 2 for a Novel HIV Vaccine Approach

Adenoviral (Ad) vectors have been used for a variety of vaccine applications including cancer and infectious diseases. Traditionally, Ad-based vaccines are designed to express antigens through transgene expression of a given antigen. However, in some cases these conventional Ad-based vaccines have had sub-optimal clinical results. These sub-optimal results are attributed in part to pre-existing Ad serotype 5 (Ad5) immunity. In order to circumvent the need for antigen expression via transgene incorporation, the “antigen capsid-incorporation” strategy has been developed and used for Ad-based vaccine development in the context of a few diseases. This strategy embodies the incorporation of antigenic peptides within the capsid structure of viral vectors. The major capsid protein hexon has been utilized for these capsid incorporation strategies due to hexon's natural role in the generation of anti-Ad immune response and its numerical representation within the Ad virion. Using this strategy, we have developed the means to incorporate heterologous peptide epitopes specifically within the major surface-exposed domains of the Ad capsid protein hexon. Our study herein focuses on generation of multivalent vaccine vectors presenting HIV antigens within the Ad capsid protein hexon, as well as expressing an HIV antigen as a transgene. These novel vectors utilize HVR2 as an incorporation site for a twenty-four amino acid region of the HIV membrane proximal ectodomain region (MPER), derived from HIV glycoprotein gp41 (gp41). Our study herein illustrates that our multivalent anti-HIV vectors elicit a cellular anti-HIV response. Furthermore, vaccinations with these vectors, which present HIV antigens at HVR2, elicit a HIV epitope-specific humoral immune response

Nová strategie pro aproximaci rozptýlených dat s využitím radiálních bázových funkcí respektující body inflexe

Aproximace rozptýlených dat je známá technika v počítačové vědě. Navrhujeme novou strategii pro umístění radiálních základních funkcí s ohledem na inflexní body. Umístění radiální základní funkce má velký vliv na kvalitu aproximace. Z tohoto důvodu navrhujeme novou strategii pro umístění radiálních základních funkcí s ohledem na vlastnosti aproximované funkce, včetně extrémních a inflexních bodů. Naše experimentální výsledky prokázaly vysokou kvalitu navrhovaného přístupu a vysokou kvalitu konečné aproximace.The approximation of scattered data is known technique in computer science. We propose a new strategy for the placement of radial basis functions respecting points of inflection. The placement of radial basis functions has a great impact on the approximation quality. Due to this fact we propose a new strategy for the placement of radial basis functions with respect to the properties of approximated function, including the extreme and the inflection points. Our experimental results proved high quality of the proposed approach and high quality of the final approximation