6 research outputs found
HIV Antigen Incorporation within Adenovirus Hexon Hypervariable 2 for a Novel HIV Vaccine Approach
Adenoviral (Ad) vectors have been used for a variety of vaccine applications including cancer and infectious diseases. Traditionally, Ad-based vaccines are designed to express antigens through transgene expression of a given antigen. However, in some cases these conventional Ad-based vaccines have had sub-optimal clinical results. These sub-optimal results are attributed in part to pre-existing Ad serotype 5 (Ad5) immunity. In order to circumvent the need for antigen expression via transgene incorporation, the âantigen capsid-incorporationâ strategy has been developed and used for Ad-based vaccine development in the context of a few diseases. This strategy embodies the incorporation of antigenic peptides within the capsid structure of viral vectors. The major capsid protein hexon has been utilized for these capsid incorporation strategies due to hexon's natural role in the generation of anti-Ad immune response and its numerical representation within the Ad virion. Using this strategy, we have developed the means to incorporate heterologous peptide epitopes specifically within the major surface-exposed domains of the Ad capsid protein hexon. Our study herein focuses on generation of multivalent vaccine vectors presenting HIV antigens within the Ad capsid protein hexon, as well as expressing an HIV antigen as a transgene. These novel vectors utilize HVR2 as an incorporation site for a twenty-four amino acid region of the HIV membrane proximal ectodomain region (MPER), derived from HIV glycoprotein gp41 (gp41). Our study herein illustrates that our multivalent anti-HIV vectors elicit a cellular anti-HIV response. Furthermore, vaccinations with these vectors, which present HIV antigens at HVR2, elicit a HIV epitope-specific humoral immune response
NovĂĄ strategie pro aproximaci rozptĂœlenĂœch dat s vyuĆŸitĂm radiĂĄlnĂch bĂĄzovĂœch funkcĂ respektujĂcĂ body inflexe
Aproximace rozptĂœlenĂœch dat je znĂĄmĂĄ technika v poÄĂtaÄovĂ© vÄdÄ. Navrhujeme novou strategii pro umĂstÄnĂ radiĂĄlnĂch zĂĄkladnĂch funkcĂ s ohledem na inflexnĂ body. UmĂstÄnĂ radiĂĄlnĂ zĂĄkladnĂ funkce mĂĄ velkĂœ vliv na kvalitu aproximace. Z tohoto dĆŻvodu navrhujeme novou strategii pro umĂstÄnĂ radiĂĄlnĂch zĂĄkladnĂch funkcĂ s ohledem na vlastnosti aproximovanĂ© funkce, vÄetnÄ extrĂ©mnĂch a inflexnĂch bodĆŻ. NaĆĄe experimentĂĄlnĂ vĂœsledky prokĂĄzaly vysokou kvalitu navrhovanĂ©ho pĆĂstupu a vysokou kvalitu koneÄnĂ© aproximace.The approximation of scattered data is known technique in computer science. We propose a new strategy for the placement of radial basis functions respecting points of inflection. The placement of radial basis functions has a great impact on the approximation quality. Due to this fact we propose a new strategy for the placement of radial basis functions with respect to the properties of approximated function, including the extreme and the inflection points. Our experimental results proved high quality of the proposed approach and high quality of the final approximation