Mjerenje raspodjele m-ksilena u tkivima štakora plinskokromatografskom analizom para iznad uzorka (head space gas chromatography)

An automated head space-gas chromatography (HS-GC) method was developed and evaluated for reliability in measurement of m-xylene in rat tissues. For tissue samples spiked with m-xylene (n=2), the analytical precision was better than 12% relative standard deviation (RSD) over the concentration range of 0.1 to cca 100 µg/g for liver and kidney, 0.1 to 170 µg/g for brain, 1.2 to 250 µg/g for fat, and 0.006 to 50 µg/mL for blood. For rats sacrificed immediately after an acute exposure to 1100 ppm of m-xylene, the relative tissue m-xylene concentrations were in the ascending order as follows: brain ≤ blood ≤kidney ≤ liver « fat. A precision of < 13% RSD was generally obtained for duplicale tissue samples from exposed animals, with m-xylene concentrations of about 10 µg/g of tissue.Razvijena je automatizirana metoda plinska head space kromatografije (izvorno: KS-GC), pouzdanost koje je provjerena mjerenjem m-ksilena u tkivima štakora. Analitička preciznost za uzorke tkiva (n-2) kojima je dodan m-ksilensneii u raspunu koncentracija od 0,1 do gotovo 100 µg/g za jetru i bubreg, uu 0,1 do 170 µg/g za mozak, od 1,2 do 250 µg/g za masno tkivo te od 0,006 do 50 µg/g za krv, pokazala se boljom od 12-postotne relativne standardne devijacije (RS0). Relativne koncentracije m-ksilena u tkivu štakora žrtvovanih odmah nakon akutne izloženosti m-ksilenu od 1100 ppm kretale su se ovim uzlaznim slijedom: mozak ≤ krv ≤ bubrcg < jetra « masno tkivo. Sveukupna preciznost iznosila je < 13% USD u paralelnim uzorcima izloženih životinja kod koncentracije m-ksilena u tkivu od oko 10 µg/g

Bending loss improvement and twisting loss studies of flexible multimode polymer waveguides

© 2020 SPIE. Multimode polymer waveguides have attracted considerable interest for use in high-speed on-board communication links as they provide low loss (40 GHz×m) and can be cost-effectively integrated onto standard PCBs. The fabrication of such waveguides on flexible substrates can provide additional advantages: shape flexibility, lightweight and reduced thickness which are particularly important in the aviation and automotive industries. Such flexible and lightweight optical connections will play an important role in next-generation airplanes and driverless cars connecting the multitude of peripheral sensors with the central processing unit at high speed and low latency. However, in such applications, flexible polymer waveguides are required to be bent to meet their stringent space requirements and twisted or stretched when connecting movable parts. Under sharp flexure, the bending or twisting loss dominates the waveguide loss limiting their practical use. In this work therefore, we present a new waveguide design for flexible polymer waveguides with improved bending performance and derive useful layout rules for minimizing twisting losses in such samples. The proposed waveguide structure only requires one additional fabrication step and achieves bending losses below 0.5 dB for a 3 mm bend. In comparison, the conventional waveguide design yields a 2 dB loss under the same bending radius and launch condition. Additionally, useful equations relating the maximum allowed number of twisting turns for low excess loss with sample thickness and width are proposed. Bending and twisting measurements on flexible waveguide samples are presented validating these methods and demonstrating the potential of this technology

High-Speed Data Transmission Over Flexible Multimode Polymer Waveguides Under Flexure

Polymer multimode waveguides on flexible substrates enable the formation of bendable low-cost optical interconnects that can be deployed in a wide range of applications. However, the highly-multimoded nature of such guides in combination with the stress and mode mixing induced due to sample bending raise important concerns about the effect that sample flexure has on their bandwidth performance and potential to support high-speed data transmission. In this work therefore, we present data transmission studies on a 1 m long flexible spiral waveguide when flexure is applied. The flexible polymer sample is bent 180° around a cylindrical mandrel and the loss and frequency response of the waveguide are obtained for radii of curvature down to 4 mm and are compared with the performance obtained when no flexure is applied. The BER performance of the respective optical link is also recorded at data rates up to 40 Gb/s. A flat frequency response up to at least 30 GHz is demonstrated for all bending radii applied and error-free (BER<10-12) data transmission is achieved for all data rates studied up to 40 Gb/s. The results clearly demonstrate that sample flexing does not result in any significant transmission impairments in such links and highlight the strong potential of this technology for use in high-speed board-level interconnections.CAPE OIC Future project, CAPE LEASA Projec

'Asexual isn't who I am': the politics of asexuality

Some literature on asexuality has claimed that it is inherently radical and contains the potential for resistance. Unfortunately, this literature has tended to be unempirical, has imagined asexuality as a disembodied entity, and has marginalised the multiple identities held by asexual people. This article, inspired by Plummer’s critical humanist approach, seeks to explore how individuals understand their asexuality to encourage forms of political action in the areas of identity, activism, online spaces, and LGBT politics. What we found was a plurality of experiences and attitudes with most adopting a pragmatic position in response to their social situation which saw large-scale political action as irrelevant. We conclude by reflecting on what these results mean for those who see asexuality as potentially radical

Subchronic toxicity of Baltic herring oil and its fractions in the rat II: Clinical observations and toxicological parameters

This study aimed to increase the knowledge about the toxicity of fish-derived organohalogen pollutants in mammals. The strategy chosen was to separate organohalogen pollutants derived from Baltic herring (Clupea harengus) fillet, in order to obtain fractions with differing proportions of identified and unidentified halogenated pollutants, and to perform a subchronic toxicity study in rats, essentially according to the OECD guidelines, at three dose levels. Nordic Sea Iodda (Mallotus villosus) oil, with low levels of persistent organohalogen pollutants, was used as an additional control diet. The toxicological examination showed that exposure to Baltic herring oil and its fractions at dose levels corresponding to a human intake in the range of 1.6 to 34.4 kg Baltic herring per week resulted in minimal effects. The spectrum of effects was similar to that, which is observed after low-level exposure to pollutants such as chlorinated dibenzo-p-dioxins and dibenzofurans (CDD/F) and chlorinated biphenyls, despite the fact that these contaminants contribute to a minor part of the extractable organically bound chlorine (EOC1). The study confirmed previous findings that induction of hepatic ethoxyresorufin deethylase (EROD) activity takes place at daily intake levels 0.15 ng fish-derived CDD/F-TEQs/kg body weight. The study also demonstrated that hepatic vitamin A reduction takes place at somewhat higher daily exposure levels, i.e. 0.16-0.30 ng fish-derived CDD/F-TEQs/kg body weight. Halogenated fatty acids, the major component of EOC1, could not be linked to any of the measured effects. From a risk management point of view, the study provides important new information of effect levels for Ah-receptor mediated responses following low level exposure to organohalogen compounds from a matrix relevant for human exposure

Establishing a nationwide emergency department-based syndromic surveillance system for better public health responses in Taiwan

Background. With international concern over emerging infectious diseases (EID) and bioterrorist attacks, public health is being required to have early outbreak detection systems. A disease surveillance team was organized to establish a hospital emergency department-based syndromic surveillance system (ED-SSS) capable of automatically transmitting patient data electronically from the hospitals responsible for emergency care throughout the country to the Centers for Disease Control in Taiwan (Taiwan-CDC) starting March, 2004. This report describes the challenges and steps involved in developing ED-SSS and the timely information it provides to improve in public health decision-making. Methods. Between June 2003 and March 2004, after comparing various surveillance systems used around the world and consulting with ED physicians, pediatricians and internal medicine physicians involved in infectious disease control, the Syndromic Surveillance Research Team in Taiwan worked with the Real-time Outbreak and Disease Surveillance (RODS) Laboratory at the University of Pittsburgh to create Taiwan's ED-SSS. The system was evaluated by analyzing daily electronic ED data received in real-time from the 189 hospitals participating in this system between April 1, 2004 and March 31, 2005. Results. Taiwan's ED-SSS identified winter and summer spikes in two syndrome groups: influenza-like illnesses and respiratory syndrome illnesses, while total numbers of ED visits were significantly higher on weekends, national holidays and the days of Chinese lunar new year than weekdays (p < 0.001). It also identified increases in the upper, lower, and total gastrointestinal (GI) syndrome groups starting in November 2004 and two clear spikes in enterovirus-like infections coinciding with the two school semesters. Using ED-SSS for surveillance of influenza-like illnesses and enteroviruses-related infections has improved Taiwan's pandemic flu preparedness and disease control capabilities. Conclusion. Taiwan's ED-SSS represents the first nationwide real-time syndromic surveillance system ever established in Asia. The experiences reported herein can encourage other countries to develop their own surveillance systems. The system can be adapted to other cultural and language environments for better global surveillance of infectious diseases and international collaboration. © 2008 Wu et al; licensee BioMed Central Ltd

Direct Generation of Neurosphere-Like Cells from Human Dermal Fibroblasts

Neural stem cell (NSC) transplantation replaces damaged brain cells and provides disease-modifying effects in many neurological disorders. However, there has been no efficient way to obtain autologous NSCs in patients. Given that ectopic factors can reprogram somatic cells to be pluripotent, we attempted to generate human NSC-like cells by reprograming human fibroblasts. Fibroblasts were transfected with NSC line-derived cellular extracts and grown in neurosphere culture conditions. The cells were then analyzed for NSC characteristics, including neurosphere formation, gene expression patterns, and ability to differentiate. The obtained induced neurosphere-like cells (iNS), which formed daughter neurospheres after serial passaging, expressed neural stem cell markers, and had demethylated SOX2 regulatory regions, all characteristics of human NSCs. The iNS had gene expression patterns that were a combination of the patterns of NSCs and fibroblasts, but they could be differentiated to express neuroglial markers and neuronal sodium channels. These results show for the first time that iNS can be directly generated from human fibroblasts. Further studies on their application in neurological diseases are warranted

The pharmacological regulation of cellular mitophagy

Small molecules are pharmacological tools of considerable value for dissecting complex biological processes and identifying potential therapeutic interventions. Recently, the cellular quality-control process of mitophagy has attracted considerable research interest; however, the limited availability of suitable chemical probes has restricted our understanding of the molecular mechanisms involved. Current approaches to initiate mitophagy include acute dissipation of the mitochondrial membrane potential (ΔΨm) by mitochondrial uncouplers (for example, FCCP/CCCP) and the use of antimycin A and oligomycin to impair respiration. Both approaches impair mitochondrial homeostasis and therefore limit the scope for dissection of subtle, bioenergy-related regulatory phenomena. Recently, novel mitophagy activators acting independently of the respiration collapse have been reported, offering new opportunities to understand the process and potential for therapeutic exploitation. We have summarized the current status of mitophagy modulators and analyzed the available chemical tools, commenting on their advantages, limitations and current applications

Phospholipase iPLA2β averts ferroptosis by eliminating a redox lipid death signal

Ferroptosis, triggered by discoordination of iron, thiols and lipids, leads to the accumulation of 15-hydroperoxy (Hp)-arachidonoyl-phosphatidylethanolamine (15-HpETE-PE), generated by complexes of 15-lipoxygenase (15-LOX) and a scaffold protein, phosphatidylethanolamine (PE)-binding protein (PEBP)1. As the Ca^{2+} -independent phospholipase A2β (iPLA2β, PLA2G6 or PNPLA9 gene) can preferentially hydrolyze peroxidized phospholipids, it may eliminate the ferroptotic 15-HpETE-PE death signal. Here, we demonstrate that by hydrolyzing 15-HpETE-PE, iPLA_{2}β averts ferroptosis, whereas its genetic or pharmacological inactivation sensitizes cells to ferroptosis. Given that PLA2G6 mutations relate to neurodegeneration, we examined fibroblasts from a patient with a Parkinson’s disease (PD)-associated mutation (fPD^{R747W}) and found selectively decreased 15-HpETE-PE-hydrolyzing activity, 15-HpETE-PE accumulation and elevated sensitivity to ferroptosis. CRISPR-Cas9-engineered Pnpla9^{R748W/R748W} mice exhibited progressive parkinsonian motor deficits and 15-HpETE-PE accumulation. Elevated 15-HpETE-PE levels were also detected in midbrains of rotenone-infused parkinsonian rats and α-synuclein-mutant Snca^{A53T} mice, with decreased iPLA2β expression and a PD-relevant phenotype. Thus, iPLA_{2}β is a new ferroptosis regulator, and its mutations may be implicated in PD pathogenesis

Acute kidney disease and renal recovery : consensus report of the Acute Disease Quality Initiative (ADQI) 16 Workgroup

Consensus definitions have been reached for both acute kidney injury (AKI) and chronic kidney disease (CKD) and these definitions are now routinely used in research and clinical practice. The KDIGO guideline defines AKI as an abrupt decrease in kidney function occurring over 7 days or less, whereas CKD is defined by the persistence of kidney disease for a period of > 90 days. AKI and CKD are increasingly recognized as related entities and in some instances probably represent a continuum of the disease process. For patients in whom pathophysiologic processes are ongoing, the term acute kidney disease (AKD) has been proposed to define the course of disease after AKI; however, definitions of AKD and strategies for the management of patients with AKD are not currently available. In this consensus statement, the Acute Disease Quality Initiative (ADQI) proposes definitions, staging criteria for AKD, and strategies for the management of affected patients. We also make recommendations for areas of future research, which aim to improve understanding of the underlying processes and improve outcomes for patients with AKD