Комплексный подход в лечении и реабилитации больных с миофаcциальным болевым синдромом лица

Determination the tactics of the treatment and rehabilitation of the patients with myofascial face pain syndrome (MFFPS). The complex of clinical and radiologic diagnostic methods to 83 patients with myofascial face pain syndrome and mandible movement disorder have performed. Patient examination have made it possible for to define succession and efforts, to reduce pain syndrome intensity, to normalize mandible movements. MFFPS treatment requires the participation of stomatologist, neurologist, roentgenologist, psychologist, therapeutist.Проведено исследование, посвященное определению тактики лечения и реабилитации пациентов с миофаcциальным болевым синдромом лица (МФБСЛ). Выполнен комплекс клинических и лучевых методов диагностики 83 пациентам с болевыми синдромами лица, сопровождающимися нарушением движений нижней челюсти. Обследование пациентов позволило определить последовательность и объем терапии, уменьшить интенсивность болевого синдрома, нормализовать движения нижней челюсти. Лечение МФБСЛ требует участия стоматологов, неврологов, рентгенологов, психологов и терапевтов-интернистов

Analysis of the dihydrofolate reductase-thymidylate synthase gene sequences in Plasmodium vivax field isolates that failed chloroquine treatment

<p>Abstract</p> <p>Background</p> <p>To use pyrimethamine as an alternative anti-malarial drug for chloroquine-resistant malaria parasites, it was necessary to determine the enzyme's genetic variation in dihydrofolate reductase-thymidylate syntase (DHFR-TS) among Korean strains.</p> <p>Methods</p> <p>Genetic variation of <it>dhfr-ts </it>genes of <it>Plasmodium vivax </it>clinical isolates from patients who did not respond to drug treatment (<it>n </it>= 11) in Korea were analysed. The genes were amplified using the polymerase chain reaction (PCR) with genomic DNA as a template.</p> <p>Results</p> <p>Sequence analysis showed that the open reading frame (ORF) of 1,857 nucleotides encoded a deduced protein of 618 amino acids (aa). Alignment with the DHFR-TS genes of other malaria parasites showed that a 231-residue DHFR domain and a 286-residue TS domain were seperated by a 101-aa linker region. This ORF shows 98.7% homology with the <it>P. vivax </it>Sal I strain (XM001615032) in the DHFR domain, 100% in the linker region and 99% in the TS domain. Comparison of the DHFR sequences from pyrimethamine-sensitive and pyrimethamine-resistant <it>P. vivax </it>isolates revealed that nine isolates belonged to the sensitive strain, whereas two isolates met the criteria for resistance. In these two isolates, the amino acid at position 117 is changed from serine to asparagine (S117N). Additionally, all Korean isolates showed a deletion mutant of THGGDN in short tandem repetitive sequences between 88 and 106 amino acid.</p> <p>Conclusions</p> <p>These results suggest that sequence variations in the DHFR-TS represent the prevalence of antifolate-resistant <it>P. vivax </it>in Korea. Two of 11 isolates have the Ser to Asn mutation in codon 117, which is the major determinant of pyrimethamine resistance in <it>P. vivax</it>. Therefore, the introduction of pyrimethamine for the treatment of chloroquine-resistant vivax malaria as alternative drug in Korea should be seriously considered.</p

FMR1 premutation and full mutation molecular mechanisms related to autism

Fragile X syndrome (FXS) is caused by an expanded CGG repeat (>200 repeats) in the 5′ un-translated portion of the fragile X mental retardation 1 gene (FMR1) leading to a deficiency or absence of the FMR1 protein (FMRP). FMRP is an RNA-binding protein that regulates the translation of a number of other genes that are important for synaptic development and plasticity. Furthermore, many of these genes, when mutated, have been linked to autism in the general population, which may explain the high comorbidity that exists between FXS and autism spectrum disorders (ASD). Additionally, premutation repeat expansions (55 to 200 CGG repeats) may also give rise to ASD through a different molecular mechanism that involves a direct toxic effect of FMR1 mRNA. It is believed that RNA toxicity underlies much of the premutation-related involvement, including developmental concerns like autism, as well as neurodegenerative issues with aging such as the fragile X-associated tremor ataxia syndrome (FXTAS). RNA toxicity can also lead to mitochondrial dysfunction, which is common in older premutation carriers both with and without FXTAS. Many of the problems with cellular dysregulation in both premutation and full mutation neurons also parallel the cellular abnormalities that have been documented in idiopathic autism. Research regarding dysregulation of neurotransmitter systems caused by the lack of FMRP in FXS, including metabotropic glutamate receptor 1/5 (mGluR1/5) pathway and GABA pathways, has led to new targeted treatments for FXS. Preliminary evidence suggests that these new targeted treatments will also be beneficial in non-fragile X forms of autism

Complex approach in the treatment and rehabilitation of the patients with myofascial face pain syndrome

Determination the tactics of the treatment and rehabilitation of the patients with myofascial face pain syndrome (MFFPS). The complex of clinical and radiologic diagnostic methods to 83 patients with myofascial face pain syndrome and mandible movement disorder have performed. Patient examination have made it possible for to define succession and efforts, to reduce pain syndrome intensity, to normalize mandible movements. MFFPS treatment requires the participation of stomatologist, neurologist, roentgenologist, psychologist, therapeutist

CLINICAL AND FUNCTIONAL SUBSTANTIATION FOR COMPLEX TREATMENT STAGING IN PATIENTS WITH THE TEMPOROMANDIBULAR JOINT DISK DISPLACEMENT WITH REDUCTION OF BITE PATHOLOGY AND THE HYPERTONICITY OF THE MASTICATORY MUSCLES

Aim of investigation: substantiation of the complex treatment phasing of patients with the temporomandibular joint disk displacement with reduction in case of bite pathology and hypertonicity of masticatory muscles. Methods. The clinical evaluation, radiological, electromyographic, and gnathological examination of 74 patients with the reduced temporomandibular joint (TMJ) disk displacement in case of occlusion pathology and chewing muscles hypertonicity have been carried out in the treatment planning and implementation from 2016 to 2018. In order to normalize the function of masticatory muscles at the first stage of treatment, relaxing splint and drug therapy were applied. The effectiveness of the chewing muscles hypertonicity correction was evaluated under electromyography control. The installation of braces for correction of malocclusion was performed after the electromyography signs normalization, reducing the frequency, severity of clicks in the TMJ and the absence of subjective complaints of discomfort or pain. Results and discussion. After the improvement of anatomical and functional relationships, planned orthodontic treatment was carried out, during which no disorders of the temporomandibular joints were revealed. The inclusion of relaxation splint for malocclusion and masticatory muscles hypertonicity, combined with the reduction of the joint disc displacement of the temporomandibular joint in the complex therapy is aimed at preventing the development of secondary osteoarthritis and blockage of the articular disc of the TMJ

Duration of spermatogenesis and identification of spermatogonial stem cell markers in a Neotropical catfish, Jundiá (Rhamdia quelen)

Spermatogenesis is a process driven by stem cell, where germ cell cycle is under the control of a specific genotype species. Considering that Jundiá (Rhamdia quelen) is a Neotropical catfish with great economical importance and useful experimental model, little information is available on basic aspects of its reproductive biology, especially on spermatogenesis. As a result, this study aimed to characterize the male germ cells, estimate the duration of spermatogenesis and evaluate the expression of selected stem cell genes in Jundiá testis. Similar to other fish species, our results showed a remarkable decrease of germ cell nuclear volume during Jundiá spermatogenesis, particularly from type A undifferentiated to late type B spermatogonia and from diplotene to late spermatids. Using a S-phase marker, bromodeoxyuridine (BrdU), the combined duration of meiotic and spermiogenic phases in this species was estimated in approximately 7 days. This is considered very short when compared to mammals, where spermatogenesis last from 30 to 74 days. Selected stem cell genes were partially sequenced and characterized in Jundiá testis. Expression analysis showed higher plzf and pou5f3 mRNA levels in the cell fractions enriched by type A undifferentiated spermatogonia. These results were further confirmed by in situ hybridization that showed strong signal of plzf and pou5f3 mRNA in type A undifferentiated spermatogonia. Altogether, these information will expand our knowledge of the reproductive biology of this species, contributing to improve its production and management, and also for biotechnological applications, such as germ cell transplantation. © 201

Y-chromosome specific YCAII, DYS19 and YAP polymorphisms in human populations: a comparative study

Two hypervariable Y-specific markers, the YCAII and DYS19 STRs, and the more stable Y Alu Polymorphism (YAP) have been analysed in about 1400 individuals of 21 different populations, mainly from Europe but also from the Middle East, Africa and Asia. On the basis of the frequency distributions of these three Y-markers we compare, using different statistical analyses, their power in detecting population genetic structure and in distinguishing closely related groups. The pattern of populations' genetic affinities inferred from the three markers considered altogether suggests a strong genetic structure that, with a few exceptions, broadly corresponds to the linguistic relatedness and/or geographic location of the sampled populations