16,975 research outputs found
Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase
The stability of eukaryotic mRNAs is dependent on a ribonucleoprotein (RNP) complex of poly(A)-binding proteins (PABPC1/Pab1) organized on the poly(A) tail. This poly(A) RNP not only protects mRNAs from premature degradation but also stimulates the Pan2-Pan3 deadenylase complex to catalyze the first step of poly(A) tail shortening. We reconstituted this process in vitro using recombinant proteins and show that Pan2-Pan3 associates with and degrades poly(A) RNPs containing two or more Pab1 molecules. The cryo-EM structure of Pan2-Pan3 in complex with a poly(A) RNP composed of 90 adenosines and three Pab1 protomers shows how the oligomerization interfaces of Pab1 are recognized by conserved features of the deadenylase and thread the poly(A) RNA substrate into the nuclease active site. The structure reveals the basis for the periodic repeating architecture at the 3' end of cytoplasmic mRNAs. This illustrates mechanistically how RNA-bound Pab1 oligomers act as rulers for poly(A) tail length over the mRNAs' lifetime.We would like to thank ... the MPIB cryo-EM, and core facilities ..
A steerable UV laser system for the calibration of liquid argon time projection chambers
A number of liquid argon time projection chambers (LAr TPC's) are being build
or are proposed for neutrino experiments on long- and short baseline beams. For
these detectors a distortion in the drift field due to geometrical or physics
reasons can affect the reconstruction of the events. Depending on the TPC
geometry and electric drift field intensity this distortion could be of the
same magnitude as the drift field itself. Recently, we presented a method to
calibrate the drift field and correct for these possible distortions. While
straight cosmic ray muon tracks could be used for calibration, multiple coulomb
scattering and momentum uncertainties allow only a limited resolution. A UV
laser instead can create straight ionization tracks in liquid argon, and allows
one to map the drift field along different paths in the TPC inner volume. Here
we present a UV laser feed-through design with a steerable UV mirror immersed
in liquid argon that can point the laser beam at many locations through the
TPC. The straight ionization paths are sensitive to drift field distortions, a
fit of these distortion to the linear optical path allows to extract the drift
field, by using these laser tracks along the whole TPC volume one can obtain a
3D drift field map. The UV laser feed-through assembly is a prototype of the
system that will be used for the MicroBooNE experiment at the Fermi National
Accelerator Laboratory (FNAL)
High resolution Ge/Li/ spectrometer reduces rate-dependent distortions at high counting rates
Modified spectrometer system with a low-noise preamplifier reduces rate-dependent distortions at high counting rates, 25,000 counts per second. Pole-zero cancellation minimizes pulse undershoots due to multiple time constants, baseline restoration improves resolution and prevents spectral shifts
Measurement of the drift field in the ARGONTUBE LAr TPC with 266~nm pulsed laser beams
ARGONTUBE is a liquid argon time projection chamber (LAr TPC) with a drift
field generated in-situ by a Greinacher voltage multiplier circuit. We present
results on the measurement of the drift-field distribution inside ARGONTUBE
using straight ionization tracks generated by an intense UV laser beam. Our
analysis is based on a simplified model of the charging of a multi-stage
Greinacher circuit to describe the voltages on the field cage rings
A method to suppress dielectric breakdowns in liquid argon ionization detectors for cathode to ground distances of several millimeters
We present a method to reach electric field intensity as high as 400 kV/cm in
liquid argon for cathode-ground distances of several millimeters. This can be
achieved by suppressing field emission from the cathode, overcoming limitations
that we reported earlier
On the Electric Breakdown in Liquid Argon at Centimeter Scale
We present a study on the dependence of electric breakdown discharge
properties on electrode geometry and the breakdown field in liquid argon near
its boiling point. The measurements were performed with a spherical cathode and
a planar anode at distances ranging from 0.1 mm to 10.0 mm. A detailed study of
the time evolution of the breakdown volt-ampere characteristics was performed
for the first time. It revealed a slow streamer development phase in the
discharge. The results of a spectroscopic study of the visible light emission
of the breakdowns complement the measurements. The light emission from the
initial phase of the discharge is attributed to electro-luminescence of liquid
argon following a current of drifting electrons. These results contribute to
set benchmarks for breakdown-safe design of ionization detectors, such as
Liquid Argon Time Projection Chambers (LAr TPC).Comment: Minor revision according to editor report. 17 pages, 15 figures, 2
tables. Turboencabulato
Experimental study of electric breakdowns in liquid argon at centimeter scale
In this paper we present results on measurements of the dielectric strength
of liquid argon near its boiling point and cathode-anode distances in the range
of 0.1 mm to 40 mm with spherical cathode and plane anode. We show that at such
distances the applied electric field at which breakdowns occur is as low as 40
kV/cm. Flash-overs across the ribbed dielectric of the high voltage
feed-through are observed for a length of 300 mm starting from a voltage of 55
kV. These results contribute to set reference for the breakdown-free design of
ionization detectors, such as Liquid Argon Time Projection Chambers (LAr TPC)
Excitation Thresholds for Nonlinear Localized Modes on Lattices
Breathers are spatially localized and time periodic solutions of extended
Hamiltonian dynamical systems. In this paper we study excitation thresholds for
(nonlinearly dynamically stable) ground state breather or standing wave
solutions for networks of coupled nonlinear oscillators and wave equations of
nonlinear Schr\"odinger (NLS) type. Excitation thresholds are rigorously
characterized by variational methods. The excitation threshold is related to
the optimal (best) constant in a class of discr ete interpolation inequalities
related to the Hamiltonian energy. We establish a precise connection among ,
the dimensionality of the lattice, , the degree of the nonlinearity
and the existence of an excitation threshold for discrete nonlinear
Schr\"odinger systems (DNLS).
We prove that if , then ground state standing waves exist if
and only if the total power is larger than some strictly positive threshold,
. This proves a conjecture of Flach, Kaldko& MacKay in
the context of DNLS. We also discuss upper and lower bounds for excitation
thresholds for ground states of coupled systems of NLS equations, which arise
in the modeling of pulse propagation in coupled arrays of optical fibers.Comment: To appear in Nonlinearit
Testing the Hubble Law with the IRAS 1.2 Jy Redshift Survey
We test and reject the claim of Segal et al. (1993) that the correlation of
redshifts and flux densities in a complete sample of IRAS galaxies favors a
quadratic redshift-distance relation over the linear Hubble law. This is done,
in effect, by treating the entire galaxy luminosity function as derived from
the 60 micron 1.2 Jy IRAS redshift survey of Fisher et al. (1995) as a distance
indicator; equivalently, we compare the flux density distribution of galaxies
as a function of redshift with predictions under different redshift-distance
cosmologies, under the assumption of a universal luminosity function. This
method does not assume a uniform distribution of galaxies in space. We find
that this test has rather weak discriminatory power, as argued by Petrosian
(1993), and the differences between models are not as stark as one might expect
a priori. Even so, we find that the Hubble law is indeed more strongly
supported by the analysis than is the quadratic redshift-distance relation. We
identify a bias in the the Segal et al. determination of the luminosity
function, which could lead one to mistakenly favor the quadratic
redshift-distance law. We also present several complementary analyses of the
density field of the sample; the galaxy density field is found to be close to
homogeneous on large scales if the Hubble law is assumed, while this is not the
case with the quadratic redshift-distance relation.Comment: 27 pages Latex (w/figures), ApJ, in press. Uses AAS macros,
postscript also available at
http://www.astro.princeton.edu/~library/preprints/pop682.ps.g
Quantitizing findings from qualitative studies for integration in mixed studies reviewing
In mixed methods reviewing, data from quantitative and qualitative studies are combined at the review level. One possible way to combine findings of quantitative and qualitative studies is to quantitize qualitative findings prior to their incorporation in a quantitative review. There are only a few examples of the quantification of qualitative findings within this context. This study adds to current research on mixed methods review methodology by reporting the pilot implementation of a new four‐step quantitizing approach. We report how we extract and quantitize the strength of relationships found in qualitative studies by assigning correlations to vague quantifiers in text fragments. This article describes (a) how the analysis is prepared; (b) how vague quantifiers in text fragments are organized and transformed to numerical values; (c) how qualitative studies as a whole are assigned effect sizes; and (d) how the overall mean effects size and variance can be calculated. The pilot implementation shows how findings from 26 primary qualitative studies are transformed into mean effect sizes and corresponding variances
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