A biophysical perspective on receptor-mediated virus entry with a focus on HIV

As part of their entry and infection strategy, viruses interact with specific receptor molecules expressed on the surface of target cells. The efficiency and kinetics of the virus-receptor interactions required for a virus to productively infect a cell is determined by the biophysical properties of the receptors, which are in turn influenced by the receptors' plasma membrane (PM) environments. Currently, little is known about the biophysical properties of these receptor molecules or their engagement during virus binding and entry. Here we review virus-receptor interactions focusing on the human immunodeficiency virus type 1 (HIV), the etiological agent of acquired immunodeficiency syndrome (AIDS), as a model system. HIV is one of the best characterised enveloped viruses, with the identity, roles and structure of the key molecules required for infection well established. We review current knowledge of receptor-mediated HIV entry, addressing the properties of the HIV cell-surface receptors, the techniques used to measure these properties, and the macromolecular interactions and events required for virus entry. We discuss some of the key biophysical principles underlying receptor-mediated virus entry and attempt to interpret the available data in the context of biophysical mechanisms. We also highlight crucial outstanding questions and consider how new tools might be applied to advance understanding of the biophysical properties of viral receptors and the dynamic events leading to virus entry

Un método integrado de actualización de modelos de elementos finitos utilizando datos procedentes del análisis modal experimental

En este artículo se presenta una técnica completa de actualización de modelos de elementos finitos de acuerdo con los resultados obtenidos con el análisis modal experimental, incidiéndose en la integración del proceso y en la necesidad de calidad de los datos de partida.In this article, a complete updating technique for finite element models on the basis of results from experimental modal analysis is presented, with attention focused on the integration of the process and on the necessity for quality in the basic data.Peer Reviewe

Decoherence reduction via continuous dynamical decoupling: Analytical study of the role of the noise spectrum

We analyze the robust character against non-static noise of clock transitions implemented via a method of continuous dynamical decoupling (CDD) in a hyperfine Zeeman multiplet in ^{87}\textrm{Rb}. The emergence of features specific to the quadratic corrections to the linear Zeeman effect is evaluated. Our analytical approach, which combines methods of stochastic analysis with time-dependent perturbation theory, allows tracing the decoherence process for generic noise sources. Working first with a basic CDD scheme, it is shown that the amplitude and frequency of the (driving) field of control can be appropriately chosen to force the non-static random input to have a (time-dependent) perturbative character. Moreover, in the dressed-state picture, the effect of noise is described in terms of an operative random variable whose properties, dependent on the driving field, can be analytically characterized. In this framework, the relevance of the spectral density of the fluctuations to the performance of the CDD technique is precisely assessed. In particular, the range of noise correlation times where the method of decoherence reduction is still efficient is identified. The results obtained in the basic CDD framework are extrapolated to concatenated schemes. The generality of our approach allows its applicability beyond the specific atomic system considered

Experiments on a videotape atom chip: fragmentation and transport studies

This paper reports on experiments with ultra-cold rubidium atoms confined in microscopic magnetic traps created using a piece of periodically-magnetized videotape mounted on an atom chip. The roughness of the confining potential is studied with atomic clouds at temperatures of a few microKelvin and at distances between 30 and 80 microns from the videotape-chip surface. The inhomogeneities in the magnetic field created by the magnetized videotape close to the central region of the chip are characterized in this way. In addition, we demonstrate a novel transport mechanism whereby we convey cold atoms confined in arrays of videotape magnetic micro-traps over distances as large as ~ 1 cm parallel to the chip surface. This conveying mechanism enables us to survey the surface of the chip and observe potential-roughness effects across different regions.Comment: 29 pages, 22 figures

Simple and fast method for fabrication of endoscopic implantable sensor arrays

Here we have developed a simple method for the fabrication of disposable implantable all-solid-state ion-selective electrodes (ISE) in an array format without using complex fabrication equipment or clean room facilities. The electrodes were designed in a needle shape instead of planar electrodes for a full contact with the tissue. The needle-shape platform comprises 12 metallic pins which were functionalized with conductive inks and ISE membranes. The modified microelectrodes were characterized with cyclic voltammetry, scanning electron microscope (SEM), and optical interferometry. The surface area and roughness factor of each microelectrode were determined and reproducible values were obtained for all the microelectrodes on the array. In this work, the microelectrodes were modified with membranes for the detection of pH and nitrate ions to prove the reliability of the fabricated sensor array platform adapted to an endoscope

Management of Virtual Machines on Globus Grids Using GridWay

Virtual machines are a promising technology to over-come some of the problems found in current Grid infras-tructures, like heterogeneity, performance partitioning or application isolation. In this work, we present an straight-forward deployment of virtual machines in Globus Grids. This solution is based on standard services and does not re-quire additional middleware to be installed. Also, we assess the suitability of this deployment in the execution of a high throughput scientific application, the XMM-Newton Scien-tific Analysis System

Coexistence of antiferro- and ferrimagnetism in the spinel ZnFe2O4 with an inversion degree d lower than 0.3

Samples with inversion parameter values (d) ranging from 0.27 to 0.14 while maintaining the crystallite size value have been successfully fabricated from commercially available powders by mechanical grinding and thermal annealing treatments at temperatures ranging between 400 and 600 °C. Detailed characterization studies of these samples using X-ray, neutron diffraction and magnetic measurements have confirmed for the first time the simultaneous coexistence at 2 K of short range antiferromagnetic and ferrimagnetic ordering for a wide range of the inversion parameter. The magnetic phase diagram obtained is different from the one previously reported, which shows at 2 K the coexistence of long range antiferromagnetic order and short range order for values of inversion parameters less than 0.1 and the presence of a ferrimagnetic order only for values of d > 0.2. At room temperature, the Rietveld analysis of NPD patterns and the magnetization curves showed a paramagnetic behavior in the samples with d = 0.1. For the samples with higher cationic inversion, typical hysteresis curves of ferrimagnetic materials were observed and the saturation magnetization values obtained agree quite well with the net magnetic moment obtained from the Rietveld refinement of the neutron diffraction patterns. © 202

Single-Molecule Super-Resolution Imaging of T-Cell Plasma Membrane CD4 Redistribution upon HIV-1 Binding

The first step of cellular entry for the human immunodeficiency virus type-1 (HIV-1) occurs through the binding of its envelope protein (Env) with the plasma membrane receptor CD4 and co-receptor CCR5 or CXCR4 on susceptible cells, primarily CD4+ T cells and macrophages. Although there is considerable knowledge of the molecular interactions between Env and host cell receptors that lead to successful fusion, the precise way in which HIV-1 receptors redistribute to sites of virus binding at the nanoscale remains unknown. Here, we quantitatively examine changes in the nanoscale organisation of CD4 on the surface of CD4+ T cells following HIV-1 binding. Using singlemolecule super-resolution imaging, we show that CD4 molecules are distributed mostly as either individual molecules or small clusters of up to 4 molecules. Following virus binding, we observe a local 3-to-10-fold increase in cluster diameter and molecule number for virus-associated CD4 clusters. Moreover, a similar but smaller magnitude reorganisation of CD4 was also observed with recombinant gp120. For one of the first times, our results quantify the nanoscale CD4 reorganisation triggered by HIV-1 on host CD4+ T cells. Our quantitative approach provides a robust methodology for characterising the nanoscale organisation of plasma membrane receptors in general with the potential to link spatial organisation to function

Atom chip for BEC interferometry

We have fabricated and tested an atom chip that operates as a matter wave interferometer. In this communication we describe the fabrication of the chip by ion-beam milling of gold evaporated onto a silicon substrate. We present data on the quality of the wires, on the current density that can be reached in the wires and on the smoothness of the magnetic traps that are formed. We demonstrate the operation of the interferometer, showing that we can coherently split and recombine a Bose–Einstein condensate with good phase stability