Concentration and localization of zinc during seed development and germination in wheat

In a field experiment, the effect of foliar Zn applications on the concentration of Zn in seeds of a bread wheat cultivar (Triticum aestivum L. cv. Balatilla) was studied during different stages of seed development. In addition, a staining method using dithizone (DTZ: diphenyl thiocarbazone) was applied to (1) study the localization of Zn in seeds, (2) follow the remobilization of Zn during germination, and (3) develop a rapid visual Zn screening method for seed and flour samples. In all seed development stages, foliar Zn treatments were effective in increasing seed Zn concentration. The highest Zn concentration in the seeds was found in the first stage of seed development (around the early milk stage); after this, seed Zn concentration gradually decreased until maturity. When reacting with Zn, DTZ forms a redcolored complex. The DTZ staining of seed samples revealed that Zn is predominantly located in the embryo and aleurone parts of the seeds. After 36 h of germination, the coleoptile and roots that emerged from seeds showed very intensive red color formation and had Zn concentrations up to 200 mg kg1, indicating a substantial remobilization of Zn from seed pools into the developing roots (radicle) and coleoptile. The DTZ staining method seems to be useful in ranking flour samples for their Zn concentrations. There was a close relationship between the seed Zn concentrations and spectral absorbance of the methanol extracts of the flour samples stained with DTZ. The results suggest that (1) accumulation of Zn in seeds is particularly high during early seed development, (2) Zn is concentrated in the embryo and aleurone parts, and (3) the DTZ staining method can be used as a rapid, semiquantitative method to estimate Zn concentrations of flour and seed samples and to screen genotypes for their Zn concentrations in seeds

On the role of initial coherence in the spin phase-space entropy production rate

Recent studies have pointed out the intrinsic dependence of figures of merit of thermodynamic relevance—such as work, heat and entropy production—on the amount of quantum coherences that is made available to a system. However, whether coherences hinder or enhance the value taken by such quantifiers of thermodynamic performance is yet to be ascertained. We show that, when considering entropy production generated in a process taking a finite-size bipartite quantum system out of equilibrium through local non-unitary channels, no general monotonicity relationship exists between the entropy production and degree of quantum coherence in the state of the system. A direct correspondence between such quantities can be retrieved when considering specific forms of open-system dynamics applied to suitably chosen initial states. Our results call for a systematic study of the role of genuine quantum features in the non-equilibrium thermodynamics of quantum processes

Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations

Micronutrient malnutrition afflicts over three billion peopleworldwide and the numbers are continuously increasing. Developing genetically micronutrientenriched cereals, which are the predominant source of human dietary, is essential to alleviate malnutrition worldwide. Wheat chromosome 6B derived from wild emmerwheat [Triticum turgidum ssp. dicoccoides (Körn.) Thell] was previously reported to be a source for high Zn concentration in the grain. In the present study, recombinant chromosome substitution lines (RSLs), previously constructed for genetic and physical maps of Gpc-B1 (a 250-kb locus affecting grain protein concentration), were used to identify the effects of the Gpc-B1 locus on grain micronutrient concentrations. RSLs carrying the Gpc-B1 allele of T. dicoccoides accumulated on average 12% higher concentration of Zn, 18% higher concentration of Fe, 29% higher concentration of Mn and 38% higher concentration of protein in the grain as compared with RSLs carrying the allele from cultivated wheat (Triticum durum). Furthermore, the high grain Zn, Fe and Mn concentrations were consistently expressed in five different environments with an absence of genotype by environment interaction. The results obtained in the present study also confirmed the previously reported effect of the wild-type allele of Gpc-B1 on earlier senescence of flag leaves. We suggest that the Gpc-B1 locus is involved in more efficient remobilization of protein, zinc, iron and manganese from leaves to the grains, in addition to its effect on earlier senescence of the green tissues

Leaf-applied sodium chloride promotes cadmium accumulation in durum wheat grain

Cadmium (Cd) accumulation in durum wheat grain is a growing concern. Among the factors affecting Cd accumulation in plants, soil chloride (Cl) concentration plays a critical role. The effect of leaf NaCl application on grain Cd was studied in greenhouse-grown durum wheat (Triticum turgidum L. durum, cv. Balcali-2000) by immersing (10 s) intact flag leaves into Cd and/or NaCl-containing solutions for 14 times during heading and dough stages. Immersing flag leaves in solutions containing increasing amount of Cd resulted in substantial increases in grain Cd concentration. Adding NaCl alone or in combination with the Cd-containing immersion solution promoted accumulation of Cd in the grains, by up to 41%. In contrast, Zn concentrations of grains were not affected or even decreased by the NaCl treatments. This is likely due to the effect of Cl complexing Cd and reducing positive charge on the metal ion, an effect that is much smaller for Zn. Charge reduction or removal (CdCl2 0 species) would increase the diffusivity/lipophilicity of Cd and enhance its capability to penetrate the leaf epidermis and across membranes. Of even more significance to human health was the ability of Cl alone to penetrate leaf tissue and mobilize and enhance shoot Cd transfer to grains, yet reducing or not affecting Zn transfer

Quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat 3 wild emmer wheat RIL population

Mineral nutrient malnutrition, and particularly deficiency in zinc and iron, afflicts over 3 billion people worldwide. Wild emmer wheat, Triticum turgidum ssp. dicoccoides, genepool harbors a rich allelic repertoire for mineral nutrients in the grain. The genetic and physiological basis of grain protein, micronutrients (zinc, iron, copper and manganese) and macronutrients (calcium, magnesium, potassium, phosphorus and sulfur) concentration was studied in tetraploid wheat population of 152 recombinant inbred lines (RILs), derived from a cross between durum wheat (cv. Langdon) and wild emmer (accession G18-16). Wide genetic variation was found among the RILs for all grain minerals, with considerable transgressive effect. A total of 82 QTLs were mapped for 10 minerals with LOD score range of 3.2–16.7. Most QTLs were in favor of the wild allele (50 QTLs). Fourteen pairs of QTLs for the same trait were mapped to seemingly homoeologous positions, reflecting synteny between the A and B genomes. Significant positive correlation was found between grain protein concentration (GPC), Zn, Fe and Cu, which was supported by significant overlap between the respective QTLs, suggesting common physiological and/or genetic factors controlling the concentrations of these mineral nutrients. Few genomic regions (chromosomes 2A, 5A, 6B and 7A) were found to harbor clusters of QTLs for GPC and other nutrients. These identified QTLs may facilitate the use of wild alleles for improving grain nutritional quality of elite wheat cultivars, especially in terms of protein, Zn and Fe

Quantitative trait loci analysis of zinc efficiency and grain zinc concentration in wheat using whole genome average interval mapping

Zinc (Zn) deficiency is a widespread problem which reduces yield and grain nutritive value in many cereal growing regions of the world. While there is considerable genetic variation in tolerance to Zn deficiency (also known as Zn efficiency), phenotypic selection is difficult and would benefit from the development of molecular markers. A doubled haploid population derived from a cross between the Zn inefficient genotype RAC875-2 and the moderately efficient genotype Cascades was screened in three experiments to identify QTL linked to growth under low Zn and with the concentrations of Zn and iron (Fe) in leaf tissue and in the grain. Two experiments were conducted under controlled conditions while the third examined the response to Zn in the field. QTL were identified using an improved method of analysis, whole genome average interval mapping. Shoot biomass and shoot Zn and Fe concentrations showed significant negative correlations, while there were significant genetic correlations between grain Zn and Fe concentrations. Shoot biomass, tissue and grain Zn concentrations were controlled by a number of genes, many with a minor effect. Depending on the traits and the site, the QTL accounted for 12–81% of the genetic variation. Most of the QTL linked to seedling growth under Zn deficiency and to Zn and Fe concentrations were associated with height genes with greater seedling biomass associated with lower Zn and Fe concentrations. Four QTL for grain Zn concentration and a single QTL for grain Fe concentration were also identified. A cluster of adjacent QTL related to the severity of symptoms of Zn deficiency, shoot Zn concentration and kernel weight was found on chromosome 4A and a cluster of QTL associated with shoot and grain Fe concentrations and kernel weight was found on chromosome 3D. These two regions appear promising areas for further work to develop markers for enhanced growth under low Zn and for Zn and Fe uptake. Although there was no significant difference between the parents, the grain Zn concentration ranged from 29 to 43 mg kg−1 within the population and four QTL associated with grain Zn concentration were identified. These were located on chromosomes 3D, 4B, 6B and 7A and they described 92% of the genetic variation. Each QTL had a relatively small effect on grain Zn concentration but combining the four high Zn alleles increased the grain Zn by 23%. While this illustrates the potential for pyramiding genes to improve grain Zn, breeding for increased grain Zn concentration requires identification of individual QTL with large effects, which in turn requires construction and testing of new mapping populations in the future

Early time-locked gamma response and gender specificity

Cataloged from PDF version of article.The aim was to investigate whether gender is a causative factor in the gamma status according to which some individuals respond with time-locked, early gamma response, G+, while the others do not show this response, G-. The sample consisted of 42 volunteer participants (between 19 and 37 years of age with at least 9 years of education). There were 22 females and 20 males. Data were collected under the oddball paradigm. Auditory stimulation (10 ms r/f time, 50 ms duration, 65 dB SPL) consisted of target (2000 Hz; p = .20) stimuli that occurred randomly within a series of standard stimuli (1000 Hz; p = .80). Gamma responses were studied in the amplitude frequency characteristics, in the digitally filtered event-related potentials (f-ERPs) and in the distributions which were obtained using the recently developed time-frequency component analysis (TFCA) technique. Participants were classified into G+ and G- groups with a criterion of full agreement between the results of an automated gamma detection technique and expert opinion. The 2 × 2 × 2 ANOVA on f-ERPs and 2 × 2 × 2 multivariate ANOVA on TFCA distributions showed the main effect of gamma status and gender as significant, and the interaction between gamma status and gender as nonsignificant. Accordingly, individual difference in gamma status is a reliable phenomenon, but this does not depend on gender. There are conflicting findings in the literature concerning the effect of gender on ERP components (N100, P300). The present study showed that if the gamma status is not included in research designs, it may produce a confounding effect on ERP parameters. © 2005 Elsevier B.V. All rights reserved