Detecting bacterial endophytes in tropical grasses of the Brachiaria genus and determining their role in improving plant growth

Plant-growth-promoting (PGP) bacteria include a diverse group of soil bacteria thought to stimulate plant growth by various mechanisms. Brachiaria forage grasses, of African origin, are perennials that often grow under low-input conditions and are likely to harbour unique populations of PGP bacteria. Three bacterial strains that tested positive for nitrogenase reductase gene sequences (nifH) were isolated from Brachiaria hybrid CIAT 36062 and introduced into Brachiaria hybrid cv. Mulato, which also had indigenous endophytic bacteria testing positive for nifH gene sequences. Under conditions of nutrient deficiency, inoculated Mulato plants had significantly higher biomass production, chlorophyll and total nitrogen contents in leaves than do control plants and were darker green. Strains ofendophytic bacteria were then artificially introduced into Brachiaria  brizantha CIAT 6294, which does not have indigenous endophytic bacteria. Results were consistent with those obtained with artificially inoculated Mulato plants, suggesting that these endophytic bacteria do benefit plant growth. DNA sequence analysis demonstrated that the nifH gene sequences were highly similar to those from Klebsiella pneumoniae and other N2-fixing organisms and that the nif genes had consensus  sequences identical to those of other N2-fixing bacteria.Key words: Bacterial endophytes, green fluorescent protein, nitrogenase reductase, plant growth-promoting bacteria, Brachiaria

The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes.

Burkholderia species play an important ecological role related to xenobiosis, the promotion of plant growth, the biocontrol of agricultural diseases, and symbiotic and non-symbiotic biological nitrogen fixation. Here, we highlight our study as providing the first complete genome of a symbiotic strain of B. phenoliruptrix, BR3459a (=CLA1), which was originally isolated in Brazil from nodules of Mimosa flocculosa and is effective in fixing nitrogen in association with this leguminous species. Genomic comparisons with other pathogenic and non-pathogenic Burkholderia strains grouped B. phenoliruptrix BR3459a with plant-associated beneficial and environmental species, although it shares a high percentage of its gene repertoire with species of the B. cepacia complex (Bcc) and "pseudomallei" group. The genomic analyses showed that the bce genes involved in exopolysaccharide production are clustered together in the same genomic region, constituting part of the Group III cluster of non-pathogenic bacteria. Regarding environmental stresses, we highlight genes that might be relevant in responses to osmotic, heat, cold and general stresses. Furthermore, a number of particularly interesting genes involved in the machinery of the T1SS, T2SS, T3SS, T4ASS and T6SS secretion systems were identified. The xenobiotic properties of strain BR3459a were also investigated, and some enzymes involved in the degradation of styrene, nitrotoluene, dioxin, chlorocyclohexane, chlorobenzene and caprolactam were identified. The genomic analyses also revealed a large number of antibiotic-related genes, the most important of which were correlated with streptomycin and novobiocin. The symbiotic plasmid showed high sequence identity with the symbiotic plasmid of B. phymatum. Additionally, comparative analysis of 545 housekeeping genes among pathogenic and non-pathogenic Burkholderia species strongly supports the definition of a new genus for the second branch, which would include BR3459a. The analyses of B. phenoliruptrix BR3459a showed key property of fixing nitrogen that together with genes for high tolerance to environmental stresses might explain a successful strategy of symbiosis in the tropics. The strain also harbours interesting sets of genes with biotechnological potential. However, the resemblance of certain genes to those of pathogenic Burkholderia raise concerns about large-scale applications in agriculture or for bioremediation

Complete genome sequence of Burkholderia phenoliruptrix BR3459a (CLA1), a heat-tolerant, nitrogen-fixing symbiont of mimosa flocculosa.

The genus Burkholderia represents a challenge to the fields of taxonomy and phylogeny and, especially, to the understanding of the contrasting roles as either opportunistic pathogens or bacteria with biotechnological potential. Few genomes of nonpathogenic strains, especially of diazotrophic symbiotic bacteria, have been sequenced to improve understanding of the genus. Here, we contribute with the complete genome sequence of Burkholderia phenoliruptrix strain BR3459a (CLA1), an effective diazotrophic symbiont of the leguminous tree Mimosa flocculosa Burkart, which is endemic to South America

Characterizing planetary systems with SPIRou: M-dwarf planet-search survey and the multiplanet systems GJ 876 and GJ 1148

SPIRou is a near-infrared spectropolarimeter and a high-precision velocimeter. The SPIRou Legacy Survey collected data from February 2019 to June 2022, half of the time devoted to a blind search for exoplanets around nearby cool stars. The aim of this paper is to present this program and an overview of its properties, and to revisit the radial velocity (RV) data of two multiplanet systems, including new visits with SPIRou. From SPIRou data, we can extract precise RVs using efficient telluric correction and line-by-line measurement techniques, and we can reconstruct stellar magnetic fields from the collection of polarized spectra using the Zeeman-Doppler imaging method. The stellar sample of our blind search in the solar neighborhood, the observing strategy, the RV noise estimates, chromatic behavior, and current limitations of SPIRou RV measurements on bright M dwarfs are described. In addition, SPIRou data over a 2.5-year time span allow us to revisit the known multiplanet systems GJ~876 and GJ~1148. For GJ~876, the new dynamical analysis including the four planets is consistent with previous models and confirms that this system is deep in the Laplace resonance and likely chaotic. The large-scale magnetic map of GJ~876 over two consecutive observing seasons is obtained and shows a dominant dipolar field with a polar strength of 30~G, which defines the magnetic environment in which the inner planet with a period of 1.94~d is embedded. For GJ~1148, we refine the known two-planet model.Comment: accepted in A&

Perfiles cromatográficos preliminares por gc-ms de algunas especies de plantas melastomatáceas

En este trabajo se presenta la determinación de los perfiles quimitaxonómicos de 11 especies de plantas melastomatáceas en fase orgánica. Las fracciones fueron analizadas por cromatografía de gases acoplada a espectrometría de masas por ionización electrónica (CG-EM/IE), puntualizando compuestos como terpenoides, hidrocarburos saturados e insaturados, compuestos oxigenados y esteroides de valor quimitaxonómico, con el fin de clasificar estas especies según sus metabolitos secundarios

Perfiles cromatográficos preliminares por gc-ms de algunas especies de plantas melastomatáceas

En este trabajo se presenta la determinación de los perfiles quimitaxonómicos de 11 especies de plantas melastomatáceas en fase orgánica. Las fracciones fueron analizadas por cromatografía de gases acoplada a espectrometría de masas por ionización electrónica (CG-EM/IE), puntualizando compuestos como terpenoides, hidrocarburos saturados e insaturados, compuestos oxigenados y esteroides de valor quimitaxonómico, con el fin de clasificar estas especies según sus metabolitos secundarios

The SPIRou Legacy Survey Rotation period of quiet M dwarfs from circular polarization in near-infrared spectral lines: I. The SPIRou APERO analysis

Context. The rotation period of stars is an important parameter along with mass, radius, effective temperature. It is an essential parameter for any radial velocity monitoring, as stellar activity can mimic the presence of a planet at the stellar rotation period. Several methods exist to measure it, including long sequences of photometric measurements or temporal series of stellar activity indicators. Aims. Here, we use the circular polarization in near-infrared spectral lines for a sample of 43 quiet M dwarfs and compare the measured rotation periods to those obtained with other methods. Methods. From Stokes V spectropolarimetric sequences observed with SPIRou at CFHT and the data processed with the APERO pipeline, we compute the least squares deconvolution profiles using different masks of atomic stellar lines with known Land\'e factor appropriate to the effective temperature of the star. We derive the longitudinal magnetic field to examine its possible variation along the 50 to 200 observations of each star. For determining the stellar rotation period, we apply a Gaussian process regression enabling us to determine the rotation period of stars with evolving longitudinal field. Results. Among the 43 stars of our sample, we were able to measure a rotation period for 27 stars. For 8 stars, the rotation period was previously unknown. We find a good agreement of our rotation periods with periods found in the literature based on photometry and activity indicators and confirm that near-infrared spectropolarimetry is an important tool to measure rotation periods, even for magnetically quiet stars. Furthermore, we compute ages for 20 stars of our sample using gyrochronology

Optical and near-infrared stellar activity characterization of the early M dwarf Gl~205 with SOPHIE and SPIRou

The stellar activity of M dwarfs is the main limitation for discovering and characterizing exoplanets orbiting them since it induces quasi-periodic RV variations. We aim to characterize the magnetic field and stellar activity of the early, moderately active, M dwarf Gl205 in the optical and nIR domains. We obtained high-precision quasi-simultaneous spectra in the optical and nIR with the SOPHIE spectrograph and SPIRou spectropolarimeter between 2019 and 2022. We computed the RVs from both instruments and the SPIRou Stokes V profiles. We used ZDI to map the large-scale magnetic field over the time span of the observations. We studied the temporal behavior of optical and nIR RVs and activity indicators with the Lomb-Scargle periodogram and a quasi-periodic GP regression. In the nIR, we studied the equivalent width of Al I, Ti I, K I, Fe I, and He I. We modeled the activity-induced RV jitter using a multi-dimensional GP regression with activity indicators as ancillary time series. The optical and nIR RVs have similar scatter but nIR shows a more complex temporal evolution. We observe an evolution of the magnetic field topology from a poloidal dipolar field in 2019 to a dominantly toroidal field in 2022. We measured a stellar rotation period of Prot=34.4$\pm$0.5 d in the longitudinal magnetic field. Using ZDI we measure the amount of latitudinal differential rotation (DR) shearing the stellar surface yielding rotation periods of Peq=32.0$\pm$1.8 d at the stellar equator and Ppol=45.5$\pm$0.3 d at the poles. We observed inconsistencies in the activity indicators' periodicities that could be explained by these DR values. The multi-dimensional GP modeling yields an RMS of the RV residuals down to the noise level of 3 m/s for both instruments, using as ancillary time series H$\alpha$ and the BIS in the optical, and the FWHM in the nIR.Comment: 41 pages, 24 figures. Accepted for publication in A&A. Improved quality of figures and reduced size of Appendi

Near-IR and optical radial velocities of the active M-dwarf star Gl 388 (AD Leo) with SPIRou at CFHT and SOPHIE at OHP

Context: The search for extrasolar planets around the nearest M-dwarfs is a crucial step towards identifying the nearest Earth-like planets. One of the main challenges in this search is that M-dwarfs can be magnetically active and stellar activity can produce radial velocity (RV) signals that could mimic those of a planet. Aims: We aim to investigate whether the 2.2 day period observed in optical RVs of the nearby active M-dwarf star Gl 388 (AD Leo) is due to stellar activity or to a planet which co-rotates with the star as suggested in the past. Methods: We obtained quasi-simultaneous optical RVs of Gl 388 from 2019 to 2021 with SOPHIE (R$\sim$75k) at the OHP in France, and near-IR RV and Stokes V measurements with SPIRou at the CFHT (R$\sim$70k). Results: The SOPHIE RV time-series displays a periodic signal with 2.23$\pm$0.01 days period and 23.6$\pm$0.5 m/s amplitude, which is consistent with previous HARPS observations obtained in 2005-2006. The SPIRou RV time-series is flat at 5 m/s rms and displays no periodic signals. RV signals of amplitude higher than 5.3 m/s at a period of 2.23 days can be excluded with a confidence level higher than 99%. Using the modulation of the longitudinal magnetic field (Bl) measured with SPIRou, we derive a stellar rotation period of 2.2305$\pm$0.0016 days. Conclusions: SPIRou RV measurements provide solid evidence that the periodic variability of the optical RVs of Gl 388 is due to stellar activity rather than to a co-rotating planet. The magnetic activity nature of the optical RV signal is further confirmed by the modulation of Bl with the same period. The SPIRou campaign on Gl 388 demonstrates the power of near-IR RV to confirm or infirm planet candidates discovered in the optical around active stars. SPIRou observations reiterate how effective spectropolarimetry is at determining the stellar rotation period.Comment: 25 pages, 23 figures, Accepted by Astronomy and Astrophysic