Some applications of DNA databanks as an investigative tool for solving criminal cases

The introduction of molecular markers, as STR (Short Tandem Repeats) was a fundamental event in Forensic Genetics. It was possible to obtain individual genetic profiles that allowed to properly identifying individuals with small probability of error. Furthermore, police officers soon discovered the investigative value provided by DNA databanks, defined as a collection of individual DNA profiles usually used to investigate crimes and identify suspects. In this article, the authors present the most common use of these police databanks, comment their drawbacks and how to overcome them. The usage and value of police DNA databanks in special criminal situations are also explained: cold cases, familial searches and dragnets. These three particular situations are described and commented in this article. In summary, although several technical, ethical - legal and international aspects have to be improved, police DNA databanks are really powerful investigation tools that allow solving a large number of criminal cases

Inbreeding and thermal adaptation in Drosophila subobscura

Using a well-adapted Drosophila subobscura population (Avala, Serbia), a drastic experiment of inbreeding was carried out to assess whether the expected level of homozygosity could be reached or if other evolutionary forces affected the process. In general, no significant changes of inversion (or arrangement) frequencies were detected after 12 brother sister mating generations. Furthermore, no significant differences were obtained between observed and expected (under the inbreeding model) karyotypic frequencies. Thus, these results seemed to indicate that the main evolutionary factor in the experiment was inbreeding. However, in the G12 generation, complete chromosomal fixation was reached only in two out of the eight final inbred lines. In these lines, the chromosomal compositions were difficult to interpret, but they could be likely a consequence of adaptation to particular laboratory conditions (constant 18 °C, food, light period, etc.). Finally, in a second experiment, the inbred lines presented higher fertility at 18 °C than at 13 °C. Also, there was a significant line effect on fertility: inbred line number 6 (A1, J1, U1+2; U1+2+6, E8, and O3+4+7) presented the highest values, which maybe the result of an adaptation to laboratory conditions. Thus, the results obtained in our experiments reflect the adaptive potential of D. subobscura inversions

The adaptive value of chromosomal inversions and climatic change. Studies on the natural populations of Drosophila subobscura from the Balkans

The adaptive value of the Drosophila subobscura chromosomal inversion polymorphism with regard to environmental effects is well-known. However, the specific details of the inversion adaptations to the global warming scenario deserve to be analyzed. Toward this aim, polymorphism and karyotypes were studied in 574 individuals from Petnica (Serbia) in annual samples taken in June for the period 2019-2022. Comparing the results of Petnica (Cfa: humid subtropical climate) with those from Avala (Serbia: Cfb, temperate oceanic climate) and Font Groga (Barcelona, Spain; Csa: hot-summer Mediterranean climate), significant differences were observed for their chromosomal polymorphism. In Petnica, inversions from U and E chromosomes mainly reacted significantly with regard to temperature, humidity, and rainfall. Moreover, the inversion polymorphism from Petnica (2019-2022) was compared with that from 1995. In this period, a significant increase in mean and maximum temperature was observed. However, to properly explain the observed variations of inversions over time, it was necessary to carefully analyze annual seasonal changes and particular heat wave episodes. Interestingly, yearly fluctuations of U chromosome 'warm'-adapted inversions corresponded with opposite changes in 'non-thermal' inversions. Perhaps these types of inversions were not correctly defined with regard to thermal adaptation, or these fluctuations were also due to adaptations to other physical and/or biological variables. Finally, a joint study of chromosomal inversion polymorphism from many Balkan populations of D. subobscura indicated that different climatic regions presented distinct composition, including thermal-adapted inversions

Medium-term changes in Drosophila subobscura chromosomal inversion polymorphism: a possible relation with global warming?

Drosophila subobscura is a species with a rich chromosomal polymorphism for inversions. Evidence demonstrates that it is adaptive. In the present research, we studied whether it is possible to detect changes in the inversion chromosomal polymorphism of D. subobscura in a medium-term period of time. The Serbian population of Avala was selected and its inversion composition in 2004 and 2011 (a seven year period) was compared. Significant variation was found in the U chromosome. This result was due to a significant increase of U1+2 (warm) and a decrease of Ust (cold) and U1+2+6. Further, minimum, maximum and mean temperatures increased (although not significantly). Thus, U chromosome seems to be able to react in a medium-term to temperature changes in the way expected by the global warming

Com s'adapten els organismes al canvi climàtic? Les inversions cromosòmiques de Drosophila subobscura: el cas de les poblacions de Sèrbia

És conegut el fet que les inversions cromosòmiques de les poblacions naturals de l'espècie Drosophila subobscura són adaptatives respecte a les variacions de l'ambient. S'ha observat que les inversions canvien en freqüència al llarg del temps segons la predicció de l'escalfament global del nostre planeta. Aquests resultats es posen de manifest en els nostres estudis realitzats a poblacions sèrbies de D. subobscura. Així, el polimorfisme cromosòmic per inversions canvia segons les estacions de l'any i també a llarg termini (períodes de 10-15 anys) d'acord amb el que s'esperaria segons l'escalfament global. Per tant, les inversions cromosòmiques d'aquesta espècie són uns bons indicadors del canvi climàtic i també són útils per estudiar com els organismes s'hi poden adaptar

How do organisms adapt to climate change?

It is well known that chromosomal inversions in natural populations of the species Drosophila subobscura are adaptations to changes in the environment. It has been observed that inversions change in frequency over time and that they are so far following the pattern expected given the global warming of our planet. These results have been brought to light by our research on Serbian populations of D. subobscura. We found that inversion-related changes in chromosomal polymorphisms depended on the season of the year and in the long term (10-15 year periods) they followed what we would expect given global warming. Therefore, chromosomal inversions in this species are good indicators of climate change and can help us to study how organisms adapt to it

Chromosomal polymorphism of D. subobscura: no differences between wild males and sons of wild females

When analyzing the chromosomal polymorphism of D. subobscura natural populations it is assumed that the information provided by wild males and sons of wild females is equivalent. Thus, using both in the analysis it is possible to increase the sample size. However, it is important to verify whether there are significant differences between both groups or not. The aim of this research has been to statistically compare the results of chromosomal polymorphism of both groups. We have used data from Avala Mountain (Serbia) where D. subobscura flies were collected from the 30th May to the 5th June 2011. Avala is located 18 km south of Belgrade and the trapping place is a forest with polydominant communities of Fagetum submontanum Table 1. Number and percentage of adult flies collected in Font Groga (Barcelona, Spain) on 9th October 2013. Males and sons of wild females were crossed with virgin females of the Küsnacht strain. Third instar larvae from F1 were dissected to obtain the salivary glands and the polytene chromosomes were stained and squashed in aceto-orcein solution. No significant differences were observed for any chromosome of the karyotype: A (p-value = 0.485), J (p-value = 0.230), U (p-value =0.572), E (p-value = 0.536), and O (p-value = 0.338). Thus, it seems that the two groups can be grouped together to obtain the chromosomal polymorphism of the population

Chromosomal Thermal Index: a comprehensive way to integrate the thermal adaptation of Drosophila subobscura whole karyotype

Drosophila has demonstrated to be an excellent model to study the adaptation of organisms to global warming, with inversion chromosomal polymorphism having a key role in this adaptation. Here, we introduce a new index (Chromosomal Thermal Index or CTI) to quantify the thermal adaptation of a population according to its composition of 'warm' and 'cold' adapted inversions. This index is intuitive, has good statistical properties, and can be used to hypothesis on the effect of global warming on natural populations. We show the usefulness of CTI using data from European populations of D. subobscura, sampled in different years. Out of 15 comparisons over time, nine showed significant increase of CTI, in accordance with global warming expectations. Although large regions of the genome outside inversions contain thermal adaptation genes, our results show that the total amount of warm or cold inversions in populations seems to be directly involved in thermal adaptation, whereas the interactions between the inversions content of homologous and non-homologous chromosomes are not relevant

Concomitant multiple anomalies of renal vessels and collecting system

Although anomalies of renal vessels and collecting system are relatively frequent, their concomitant occurrence is a rare event. During dissection of a 75-year-old male formalin-embalmed cadaver, we found multiple variations in the renal vessels and renal collecting system. Both kidneys were normal in size and anteriorly malrotated, with duplex collecting system and duplex ureter. One ureter drained the upper part of the kidney and the second ureter drained the lower part of the kidney. Superior and inferior collecting systems were separated by renal parenchyma. The right kidney had two renal arteries, the first renal artery (main renal artery) originating from the abdominal aorta, passing behind the inferior vena cava (IVC) and entering the kidney through the superior and inferior renal hilum. The second artery was the inferior polar artery. In addition, the right kidney had two renal veins as well. Three renal tributaries emerged from the upper and lower portion of the right renal hilum, and they joined to form the main renal vein which drained into the IVC. The lower renal vein was the inferior polar vein. The left kidney had four renal arteries (two hilar arteries and two polar arteries). The main left renal vein emerged from both superior and inferior left renal hilum, passed in front of the abdominal aorta and drained into the IVC. The left kidney also had the inferior polar vein which was divided behind the aorta (retro aortic vein) into two venous trunks. These venous trunks drained separately into posteromedialaspect of the IVC. Finally, the right testicular vein was formed by two tributaries and drained into the IVC, whereas the two left testicular veins drained separately into the left main renal vein

Metagenome-based diversity analyses suggest a significant contribution of non-cyanobacterial lineages to carbonate precipitation in modern microbialites

Frontiers in Microbiology 6 (2015): 797 This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permissionCyanobacteria are thought to play a key role in carbonate formation due to their metabolic activity, but other organisms carrying out oxygenic photosynthesis (photosynthetic eukaryotes) or other metabolisms (e.g., anoxygenic photosynthesis, sulfate reduction), may also contribute to carbonate formation. To obtain more quantitative information than that provided by more classical PCR-dependent methods, we studied the microbial diversity of microbialites from the Alchichica crater lake (Mexico) by mining for 16S/18S rRNA genes in metagenomes obtained by direct sequencing of environmental DNA. We studied samples collected at the Western (AL-W) and Northern (AL-N) shores of the lake and, at the latter site, along a depth gradient (1, 5, 10, and 15 m depth). The associated microbial communities were mainly composed of bacteria, most of which seemed heterotrophic, whereas archaea were negligible. Eukaryotes composed a relatively minor fraction dominated by photosynthetic lineages, diatoms in AL-W, influenced by Si-rich seepage waters, and green algae in AL-N samples. Members of the Gammaproteobacteria and Alphaproteobacteria classes of Proteobacteria, Cyanobacteria, and Bacteroidetes were the most abundant bacterial taxa, followed by Planctomycetes, Deltaproteobacteria (Proteobacteria), Verrucomicrobia, Actinobacteria, Firmicutes, and Chloroflexi. Community composition varied among sites and with depth. Although cyanobacteria were the most important bacterial group contributing to the carbonate precipitation potential, photosynthetic eukaryotes, anoxygenic photosynthesizers and sulfate reducers were also very abundant. Cyanobacteria affiliated to Pleurocapsales largely increased with depth. Scanning electron microscopy (SEM) observations showed considerable areas of aragonite-encrusted Pleurocapsa-like cyanobacteria at microscale. Multivariate statistical analyses showed a strong positive correlation of Pleurocapsales and Chroococcales with aragonite formation at macroscale, and suggest a potential causal link. Despite the previous identification of intracellularly calcifying cyanobacteria in Alchichica microbialites, most carbonate precipitation seems extracellular in this systemWe are grateful to Eleonor Cortés for help and good company during the field trip and to Eberto Novelo for helpful discussions at the UNAM lab. This research was funded by the European Research Council Grants ProtistWorld (PI PL-G., Grant Agreement no. 322669) and CALCYAN (PI KB, Grant Agreement no. 307110) under the European Union’s Seventh Framework Program and the RTP Génomique environnementale of the CNRS (project MetaStrom, PI DM