Characterization of the primary matabolome of Brachystegia boehmii and Colophospermum mopane under different fire regimes in Miombo and Mopane African woodlands

Original ResearchMiombo and Mopane are ecological and economic important woodlands from Africa, highly affected by a combination of climate change factors, and anthropogenic fires. Although most species of these ecosystems are fire tolerant, the mechanisms that lead to adaptive responses (metabolic reconfiguration) are unknown. In this context, the aim of this study was to characterize the primary metabolite composition of typical legume trees from these ecosystems, namely, Brachystegia boehmii (Miombo) and Colophospermum mopane (Mopane) subjected to different fire regimes. Fresh leaves from each species were collected in management units and landscapes across varied fire frequencies in the Niassa National Reserve (NNR) and Limpopo National Park (LNP) in Mozambique. Primary metabolites were extracted and analyzed with a well-established gas chromatography time-of-flight mass spectrometry metabolomics platform (GC-TOF-MS). In B. boehmii, 39 primary metabolites were identified from which seven amino acids, two organic acids and two sugars increased significantly, whereas in C. mopane, 41 primary metabolites were identified from which eight amino acids, one sugar and two organic acids significantly increased with increasing fire frequency. The observed changes in the pool of metabolites of C. mopane might be related to high glycolytic and tricarboxylic acid (TCA) rate, which provided increased levels of amino acids and energy yield. In B. boehmii, the high levels of amino acids might be due to inhibition of protein biosynthesis. The osmoprotectant and reactive oxygen species (ROS) scavenging properties of accumulated metabolites in parallel with a high-energy yield might support plants survival under fire stressinfo:eu-repo/semantics/publishedVersio

Intensificação ecológica da agricultura: uma opção para a preservação ambiental com lucratividade.

As perspectivas futuras sugerem que uma verdadeira revolução nos processos de produção agrícola terá que acontecer. Se por um lado o modelo produtivista, oriundo da revolução verde, tem mostrado seus limites, principalmente no que diz respeito ao uso insustentável de recursos naturais e nos impactos negativos que causam ao meio ambiente, por outro, estima-se que a população mundial deverá chegar a 9 bilhões de pessoas em 2050, aumentando assim a demanda por alimentos, fibras, madeiras e, junte-se a essa lista: biocombustíveis. Esse aumento na demanda será ainda maior do que uma simples progressão do aumento populacional, já que uma substancial melhora na qualidade de vida das populações menos favorecidas é esperada. Essa melhora na qualidade de vida terá que passar, inevitavelmente, por um maior uso per capta de produtos provenientes da agricultura.bitstream/item/123090/1/Intensificacao-Ecologica.pd

Quality Indicators Compliance and Survival Outcomes in Breast Cancer according to Age in a Certified Center

Simple Summary In this study, we examined how age impacts the outcomes of breast cancer by comparing three age groups: patients 45 years old or younger, patients between 46 and 69 years old, and patients 70 years old or older. Despite similar cancer staging and tumor characteristics among the age groups, the study found that older patients were prone to suboptimal treatment. Older patients also had a lower overall survival rate, but this was not related to cancer itself. Instead, we found that undertreatment was a factor that negatively impacted survival for older women with breast cancer. This study suggests that tumor characteristics and treatment compliance are more important predictors of survival than chronological age. Age as a breast cancer (BC) prognostic factor remains debatable. Several studies have investigated clinicopathological features at different ages, but few make an age group direct comparison. The European Society of Breast Cancer Specialists quality indicators (EUSOMA-QIs) allow a standardized quality assurance of BC diagnosis, treatment, and follow-up. Our objective was to compare clinicopathological features, compliance to EUSOMA-QIs and BC outcomes in three age groups (= 70 years). Data from 1580 patients with staged 0-IV BC from 2015 to 2019 were analyzed. The minimum standard and desirable target on 19 mandatory and 7 recommended QIs were studied. The 5-year relapse rate, overall survival (OS), and BC-specific survival (BCSS) were also evaluated. No meaningful differences in TNM staging and molecular subtyping classification between age groups were found. On the contrary, disparities in QIs compliance were observed: 73.1% in = 70 years women. Despite a unique exception-more invasive G3 tumors in younger patients-no age-specific differences in BC biology impacting outcome were found. Although increased noncompliance in older women, no outcome correlation was observed with QIs noncompliance in any age group. Clinicopathological features and differences in multimodal treatment (not the chronological age) are predictors of lower BCSS.The article publication was supported by National Funds through FCT-Fundacao para a Ciencia e a Tecnologia, I.P., within CINTESIS, R & D Unit (reference UIDB/4255/2020) and within the scope of the project RISE, Associated Laboratory (reference LA/P/0053/2020). ASB, acknowledges FCT-supported funds from UnIC@RISE (UIDB/00051/2020 & UIDP/00051/2020)

SOPHIE velocimetry of Kepler transit candidates XIV. A joint photometric, spectroscopic, and dynamical analysis of the Kepler-117 system

As part of our follow-up campaign of Kepler planets, we observed Kepler-117 with the SOPHIE spectrograph at the Observatoire de Haute-Provence. This F8-type star hosts two transiting planets in non-resonant orbits. The planets, Kepler-117 b and c, have orbital periods $\simeq 18.8$ and $\simeq 50.8$ days, and show transit-timing variations (TTVs) of several minutes. We performed a combined Markov chain Monte Carlo (MCMC) fit on transits, radial velocities, and stellar parameters to constrain the characteristics of the system. We included the fit of the TTVs in the MCMC by modeling them with dynamical simulations. In this way, consistent posterior distributions were drawn for the system parameters. According to our analysis, planets b and c have notably different masses ($0.094 \pm 0.033$ and $1.84 \pm 0.18$ M$_{\rm J}$) and low orbital eccentricities ($0.0493 \pm 0.0062$ and $0.0323 \pm 0.0033$). The uncertainties on the derived parameters are strongly reduced if the fit of the TTVs is included in the combined MCMC. The TTVs allow measuring the mass of planet b, although its radial velocity amplitude is poorly constrained. Finally, we checked that the best solution is dynamically stable.Comment: 16 pages, of whom 5 of online material.12 figures, of whom 2 in the online material. 7 tables, of whom 4 in the online material. Published in A&

Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom.

We have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57Bl/6 > A/Sn > BALB/c > C3H/HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 microg) were injected i.p. with 50 microg of venom produced IL-6, IL-10, INF-gamma, TNF-alpha and NO in the serum. In vitro the cells from the mice injected and challenged with the venom only released IL-10 while peritoneal macrophages released IL-10, INF-gamma and less amounts of IL-6; (c) establishment of local inflammation and necrosis induced by the venom, coincides with the peaks of TNF-alpha, IFN-gamma and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor. These results suggest that susceptibility to Bothrops atrox venom is genetically dependent but MHC independent; that IL-6, IL-10, TNF-alpha, IFN-gamma and NO can be involved in the mediation of tissue damage; and that the major venom component inducers of the lesions are haemorrhagins

Calagem melhora a disponibilidade de cálcio no solo e a produtividade de Ilex paraguariensis St. Hil.

Avaliou-se a influência da calagem na disponibilidade de Ca2+ no solo, produtividade e teor foliar de Ca e Al em erva-mate. Instalaram-se experimentos em três locais, avaliando-se cinco doses superficiais de calcário dolomítico para atingir 50 % das seguintes saturações por bases: 0, 30, 60, 90 e 120 %. Após 18 meses, avaliou-se: teor de Ca2+ no solo em três profundidades; teor foliar de Ca e Al e produtividade de erva-mate comercial (ECOM). A calagem, não alterou o teor foliar de Ca, mas, reduziu o de Al. A calagem, aumentou o teor de Ca 2+ no solo e, a produtividade de ECOM entre 21 e 27 %. Em solos com baixa disponibilidade natural de Ca 2+, a calagem deve disponibilizá-lo entre 4,0-4,5 cmol c dm-3 na profundidade de 0-5 cm, já em solos com alta disponibilidade de Ca 2+, a calagem deve disponibilizar o nutriente até 6,0 cmolc dm-3 para o cultivo da erva-mate

SOPHIE velocimetry of Kepler transit candidates. XV. KOI-614b, KOI-206b, and KOI-680b: a massive warm Jupiter orbiting a G0 metallic dwarf and two highly inflated planets with a distant companion around evolved F-type stars

We report the validation and characterization of three new transiting exoplanets using SOPHIE radial velocities: KOI-614b, KOI-206b, and KOI-680b. KOI-614b has a mass of $2.86\pm0.35~{\rm M_{Jup}}$ and a radius of $1.13^{+0.26}_{-0.18}~{\rm R_{Jup}}$, and it orbits a G0, metallic ([Fe/H]=$0.35\pm0.15$) dwarf in 12.9 days. Its mass and radius are familiar and compatible with standard planetary evolution models, so it is one of the few known transiting planets in this mass range to have an orbital period over ten days. With an equilibrium temperature of $T_{eq}=1000 \pm 45$ K, this places KOI-614b at the transition between what is usually referred to as "hot" and "warm" Jupiters. KOI-206b has a mass of $2.82\pm 0.52~{\rm M_{Jup}}$ and a radius of $1.45\pm0.16~{\rm R_{Jup}}$, and it orbits a slightly evolved F7-type star in a 5.3-day orbit. It is a massive inflated hot Jupiter that is particularly challenging for planetary models because it requires unusually large amounts of additional dissipated energy in the planet. On the other hand, KOI-680b has a much lower mass of $0.84\pm0.15~{\rm M_{Jup}}$ and requires less extra-dissipation to explain its uncommonly large radius of $1.99\pm0.18~{\rm R_{Jup}}$. It is one of the biggest transiting planets characterized so far, and it orbits a subgiant F9-star well on its way to the red giant stage, with an orbital period of 8.6 days. With host stars of masses of $1.46\pm0.17~M_{\odot}$ and $1.54 \pm 0.09~M_{\odot}$, respectively, KOI-206b, and KOI-680b are interesting objects for theories of formation and survival of short-period planets around stars more massive than the Sun. For those two targets, we also find signs of a possible distant additional companion in the system

Adubação potássica e intervalos de colheita influenciam o potássio do solo e a produtividade de cultivos de erva-mate.

O trabalho objetivou avaliar a adubação potássica e intervalos de colheita na disponibilidade de potássio, produtividade e distribuição do conteúdo de potássio na massa colhida da erva-mate. Avaliaram-se doses de 0, 20, 40, 80, 160 e 320 kg ha-1 de K2O para colheitas com intervalos de 12, 18 e 24 meses. Quantificou-se a disponibilidade de potássio em duas profundidades do solo e a produtividade de erva-mate comercial (ECOM) e galho grosso (GG) e distribuição do conteúdo de K nesses componentes. A adubação potássica aumentou a disponibilidade de potássio no solo e aumentou a produtividade da erva-mate. Para cultivos em solos com disponibilidade em nível baixo de potássio, recomenda-se doses próximas a 190, 270 e 320 kg ha-1 de K2O, respectivamente, para colheitas com intervalos de 12, 18 e 24 meses. Na retirada do GG da área, requer adubação de reposição do potássio adicional entre 20 a 30 %